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Literature summary for 1.1.1.1 extracted from

  • Pennacchio, A.; Giordano, A.; Pucci, B.; Rossi, M.; Raia, C.A.
    Biochemical characterization of a recombinant short-chain NAD(H)-dependent dehydrogenase/reductase from Sulfolobus acidocaldarius (2010), Extremophiles, 14, 193-204.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1,4-dioxane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
2-mercaptoethanol 5 mM, 112% of initial activity Sulfolobus acidocaldarius
2-propanol 120–125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
acetonitrile 120–125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
n-heptane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
n-hexane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
TBME significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius

Application

Application Comment Organism
synthesis enzyme catalyses the reduction of alpha-methyl and alpha-ethyl benzoylformate, and methyl o-chlorobenzoylformate with 100% conversion to methyl (S)-mandelate [17% enantiomeric excess (ee)], ethyl (R)-mandelate (50% ee), and methyl (R)-o-chloromandelate (72% ee), respectively, with an efficient in situ NADH-recycling system which involves glucose and a thermophilic glucose dehydrogenase Sulfolobus acidocaldarius

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus acidocaldarius
overexpressed in Escherichia coli Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
1,4-dioxane 30%, 5–30% inactivation of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
2-propanol 120–125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
acetonitrile 120–125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
CoSO4 1 mM, 11% inhibition Sulfolobus acidocaldarius
Cu2+ 1 mM, 89% of initial activity Sulfolobus acidocaldarius
EDTA 1 mM, 93% of initial activity Sulfolobus acidocaldarius
FeSO4 1 mM, 20% inhibition Sulfolobus acidocaldarius
Hg(CH3COO)2 1 mM, 37% inhibition Sulfolobus acidocaldarius
Hg2+ 1 mM, 63% of initial activity Sulfolobus acidocaldarius
iodoacetate 1 mM, 12% inhibition Sulfolobus acidocaldarius
additional information the chlorides of Li+, Na+, K+, Ca2+, Mg2+, and Mn2+ do not affect the activity of the enzyme, whereas the sulfate of heavy metal ions such as Fe2+, Co2+, and Cu2+ cause a slight inactivation Sulfolobus acidocaldarius
NaCl 1 mM, 12% inhibition Sulfolobus acidocaldarius
Zn2+ 1 mM, 91% of initial activity Sulfolobus acidocaldarius
ZnSO4 1 mM, 9% inhibition Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
NADH pH 5.5, 65°C Sulfolobus acidocaldarius
0.16
-
NADH 65°C, pH 5.5 Sulfolobus acidocaldarius
0.44
-
NAD+ pH 8.0, 65°C Sulfolobus acidocaldarius
0.44
-
NAD+ 65°C, pH 8.0 Sulfolobus acidocaldarius
0.71
-
isatin pH 5.5, 65°C Sulfolobus acidocaldarius
0.71
-
isatin 65°C, pH 5.5 Sulfolobus acidocaldarius
1.05
-
(S)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.05
-
(S)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.28
-
(R)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.3
-
(R)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
2.4
-
(R)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
2.4
-
(R)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
2.8
-
methyl o-chlorobenzoylformate pH 5.5, 65°C Sulfolobus acidocaldarius
2.8
-
methyl o-chlorobenzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
4.2
-
cis-decahydro-1-naphthol 65°C, pH 8.0 Sulfolobus acidocaldarius
5.3
-
ethyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
6.4
-
(S)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
6.4
-
(S)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
6.5
-
methyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
9.3
-
cycloheptanol pH 8.0, 65°C Sulfolobus acidocaldarius
9.3
-
cycloheptanol 65°C, pH 8.0 Sulfolobus acidocaldarius
9.9
-
3-Methylcyclohexanol 65°C, pH 8.0 Sulfolobus acidocaldarius
12.1
-
1-phenyl-1,2-propanedione 65°C, pH 5.5 Sulfolobus acidocaldarius
23.5
-
2,2,2-trifluoroacetophenone 65°C, pH 5.5 Sulfolobus acidocaldarius
66
-
3-methylcyclohexanone 65°C, pH 5.5 Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ 1 mM, 106% of initial activity Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28978
-
4 * 28978, calculated from sequence Sulfolobus acidocaldarius
28978
-
4 * 28978, calculated, 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
30000
-
4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
30000
-
4 * 28978, calculated, 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
65000
-
gel filtration Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sulfolobus acidocaldarius the physiological direction of the catalytic reaction is reduction rather than oxidation ?
-
?
additional information Sulfolobus acidocaldarius DSM 639 the physiological direction of the catalytic reaction is reduction rather than oxidation ?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
1,4-dioxane 30%, 5–30% inactivation of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1,4-dioxane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
2-propanol 120–125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
acetonitrile 120–125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
dioxane 17%, 160% of initial activity Sulfolobus acidocaldarius
Ethyl acetate significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
Ethyl acetate 30%, 180% of initial activity Sulfolobus acidocaldarius
methyl-tert-butylether significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
n-heptane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
n-heptane 30%, 180% of initial activity Sulfolobus acidocaldarius
n-hexane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
tert-butylmethyl ether 30%, 210% of initial activity Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J702
-
-
Sulfolobus acidocaldarius Q4J9F2
-
-
Sulfolobus acidocaldarius DSM 639 Q4J702
-
-
Sulfolobus acidocaldarius DSM 639 Q4J9F2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.6
-
pH 5.5, 65°C Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-indanol + NAD+ 62% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
?
(R)-1-indanol + NAD+
-
Sulfolobus acidocaldarius 1-indanone + NADH + H+
-
r
(R)-alpha-tetralol + NAD+ 58% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
?
(R)-alpha-tetralol + NAD+
-
Sulfolobus acidocaldarius alpha-tetralone + NADH + H+
-
r
(S)-1-indanol + NAD+
-
Sulfolobus acidocaldarius ?
-
?
(S)-1-indanol + NAD+
-
Sulfolobus acidocaldarius 1-indanone + NADH + H+
-
r
(S)-2-pentanol + NAD+ 11% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
?
(S)-alpha-tetralol + NAD+
-
Sulfolobus acidocaldarius alpha-tetralone + NADH + H+
-
r
(S)-alpha-tetralol + NAD+
-
Sulfolobus acidocaldarius DSM 639 alpha-tetralone + NADH + H+
-
r
(S)-alpha-tetralol + NAD+ 12% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
?
1-indanone + NADH + H+
-
Sulfolobus acidocaldarius (S)-1-indanol + NAD+ 6 h, 50°C, 6% conversion, 25% enantiomeric excess r
1-indanone + NADH + H+
-
Sulfolobus acidocaldarius DSM 639 (S)-1-indanol + NAD+ 6 h, 50°C, 6% conversion, 25% enantiomeric excess r
1-phenyl-1,2-propanedione + NADH about 25% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
?
2,2,2-trifluoroacetophenone + NADH about 35% of the activity compared to isatin Sulfolobus acidocaldarius 2,2,2-trifluoro-1-phenylethanol + NAD+
-
?
3-methylcyclohexanol + NAD+ 14% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius 3-methylcyclohexanone + NADH + H+
-
?
3-methylcyclohexanone + NADH about 10% of the activity compared to isatin Sulfolobus acidocaldarius 3-methylcyclohexanol + NAD+
-
?
3-methylcyclohexanone + NADH about 10% of the activity compared to isatin Sulfolobus acidocaldarius DSM 639 3-methylcyclohexanol + NAD+
-
?
cis-decahydro-1-naphthol + NAD+ 11% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
?
cycloheptanol + NAD+
-
Sulfolobus acidocaldarius cycloheptanone + NADH + H+
-
r
cycloheptanol + NAD+ 37% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius cycloheptanone + NADH + H+
-
?
ethyl benzoylformate + NADH about 30% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
?
ethyl benzoylformate + NADH + H+
-
Sulfolobus acidocaldarius ethyl (R)-mandelate + NAD+ 50% enantiomeric excess r
ethyl benzoylformate + NADH + H+
-
Sulfolobus acidocaldarius DSM 639 ethyl (R)-mandelate + NAD+ 50% enantiomeric excess r
ethyl pyruvate + NADH about 15% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
?
ethyl pyruvate + NADH about 15% of the activity compared to isatin Sulfolobus acidocaldarius DSM 639 ?
-
?
isatin + NADH
-
Sulfolobus acidocaldarius ?
-
?
isatin + NADH + H+
-
Sulfolobus acidocaldarius ? + NAD+
-
r
isatin + NADH + H+
-
Sulfolobus acidocaldarius DSM 639 ? + NAD+
-
r
methyl benzoylformate + NADH about 35% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
?
methyl benzoylformate + NADH about 35% of the activity compared to isatin Sulfolobus acidocaldarius DSM 639 ?
-
?
methyl benzoylformate + NADH + H+
-
Sulfolobus acidocaldarius methyl (S)-mandelate + NAD+ 6 h, 100% conversion, 17% enantiomeric excess r
methyl o-chlorobenzoylformate + NADH about 55% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
?
methyl o-chlorobenzoylformate + NADH + H+
-
Sulfolobus acidocaldarius methyl (R)-o-chloromandelate + NAD+ 6 h, 99% conversion, 72% enantiomeric excess r
additional information the physiological direction of the catalytic reaction is reduction rather than oxidation Sulfolobus acidocaldarius ?
-
?
additional information the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl Sulfolobus acidocaldarius ?
-
?
additional information enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes Sulfolobus acidocaldarius ?
-
?
additional information the physiological direction of the catalytic reaction is reduction rather than oxidation Sulfolobus acidocaldarius DSM 639 ?
-
?
additional information the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl Sulfolobus acidocaldarius DSM 639 ?
-
?
additional information enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes Sulfolobus acidocaldarius DSM 639 ?
-
?

Subunits

Subunits Comment Organism
More tetramer structure results from chemical crosslinking experiments Sulfolobus acidocaldarius
tetramer 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
tetramer 4 * 28978, calculated from sequence Sulfolobus acidocaldarius
tetramer 4 * 28978, calculated, 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
SaADH
-
Sulfolobus acidocaldarius
Saci_1232
-
Sulfolobus acidocaldarius
short-chain ADH
-
Sulfolobus acidocaldarius
short-chain NAD(H)-dependent dehydrogenase/reductase
-
Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Sulfolobus acidocaldarius
75
-
-
Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 85 65°C: about 40% of maximal activity, 85°C: about 35% of maximal activity Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
24 h, 109% of initial activity Sulfolobus acidocaldarius
70
-
pH 9.0, 50 mM Tris-HCl, 24% loss of acrtivity Sulfolobus acidocaldarius
70
-
24 h, 76% of initial activity Sulfolobus acidocaldarius
90
-
half-life 30 min Sulfolobus acidocaldarius
90
-
half-inactivation temperature is 30 min Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.98
-
cis-decahydro-1-naphthol 65°C, pH 8.0 Sulfolobus acidocaldarius
2 8 NADH pH 5.5, 65°C Sulfolobus acidocaldarius
2 8 NADH 65°C, pH 5.5 Sulfolobus acidocaldarius
2.1
-
(S)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
2.1
-
(S)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
2.35
-
ethyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
3.1
-
1-phenyl-1,2-propanedione 65°C, pH 5.5 Sulfolobus acidocaldarius
3.7
-
NAD+ pH 8.0, 65°C Sulfolobus acidocaldarius
3.7
-
NAD+ 65°C, pH 8.0 Sulfolobus acidocaldarius
3.8
-
3-methylcyclohexanone 65°C, pH 5.5 Sulfolobus acidocaldarius
4.4
-
cycloheptanol pH 8.0, 65°C Sulfolobus acidocaldarius
4.4
-
cycloheptanol 65°C, pH 8.0 Sulfolobus acidocaldarius
6.2
-
methyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
7.1
-
(R)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
7.1
-
(R)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
9.2
-
methyl o-chlorobenzoylformate pH 5.5, 65°C Sulfolobus acidocaldarius
9.2
-
methyl o-chlorobenzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
10
-
2,2,2-trifluoroacetophenone 65°C, pH 5.5 Sulfolobus acidocaldarius
13.5
-
(R)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
13.5
-
(R)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
13.7
-
(S)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
13.7
-
(S)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
22
-
isatin pH 5.5, 65°C Sulfolobus acidocaldarius
22
-
isatin 65°C, pH 5.5 Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
reduction reaction Sulfolobus acidocaldarius
5.1
-
reduction of ketones Sulfolobus acidocaldarius
5.5
-
assay at Sulfolobus acidocaldarius
8.2
-
oxidation reaction Sulfolobus acidocaldarius
8.2
-
oxidation of alcohols Sulfolobus acidocaldarius

pH Range

pH Minimum pH Maximum Comment Organism
4.9 5.8 pH 4.9: about 25% of maximal activity, pH 5.8: about 40% of maximal activity, reduction reaction Sulfolobus acidocaldarius
6.7 8.5 pH 6.7: about 40% of maximal activity, pH 8.5: about 85% of maximal activity, oxidation reaction Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme transfers the deuteride to the Si-face of NAD+ Sulfolobus acidocaldarius
NADH strictly required Sulfolobus acidocaldarius
NADH the specificity constant value is 21-fold higher for NADH than NAD+. No activity with NADP(H) Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.06
-
3-methylcyclohexanone 65°C, pH 5.5 Sulfolobus acidocaldarius
0.23
-
cis-decahydro-1-naphthol 65°C, pH 8.0 Sulfolobus acidocaldarius
0.25
-
1-phenyl-1,2-propanedione 65°C, pH 5.5 Sulfolobus acidocaldarius
0.42
-
2,2,2-trifluoroacetophenone 65°C, pH 5.5 Sulfolobus acidocaldarius
0.44
-
ethyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
0.47
-
cycloheptanol 65°C, pH 8.0 Sulfolobus acidocaldarius
0.47
-
cycloheptanol pH 8.0, 65°C Sulfolobus acidocaldarius
0.95
-
methyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
2
-
(S)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
2
-
(S)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
2.14
-
(S)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
2.14
-
(S)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
2.98
-
(R)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
2.98
-
(R)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
3.3
-
methyl o-chlorobenzoylformate pH 5.5, 65°C Sulfolobus acidocaldarius
3.3
-
methyl o-chlorobenzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
8.4
-
NAD+ 65°C, pH 8.0 Sulfolobus acidocaldarius
8.4
-
NAD+ pH 8.0, 65°C Sulfolobus acidocaldarius
10.5
-
(R)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
10.5
-
(R)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
31
-
isatin pH 5.5, 65°C Sulfolobus acidocaldarius
31
-
isatin 65°C, pH 5.5 Sulfolobus acidocaldarius
175
-
NADH pH 5.5, 65°C Sulfolobus acidocaldarius
175
-
NADH 65°C, pH 5.5 Sulfolobus acidocaldarius