Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,4-dioxane | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius | |
2-mercaptoethanol | 5 mM, 112% of initial activity | Sulfolobus acidocaldarius | |
2-propanol | 120125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 530% of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius | |
acetonitrile | 120125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 530% of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius | |
n-heptane | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius | |
n-hexane | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius | |
TBME | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius |
Application | Comment | Organism |
---|---|---|
synthesis | enzyme catalyses the reduction of alpha-methyl and alpha-ethyl benzoylformate, and methyl o-chlorobenzoylformate with 100% conversion to methyl (S)-mandelate [17% enantiomeric excess (ee)], ethyl (R)-mandelate (50% ee), and methyl (R)-o-chloromandelate (72% ee), respectively, with an efficient in situ NADH-recycling system which involves glucose and a thermophilic glucose dehydrogenase | Sulfolobus acidocaldarius |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus acidocaldarius |
overexpressed in Escherichia coli | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-dioxane | 30%, 530% inactivation of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius | |
2-propanol | 120125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 530% of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius | |
acetonitrile | 120125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 530% of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius | |
CoSO4 | 1 mM, 11% inhibition | Sulfolobus acidocaldarius | |
Cu2+ | 1 mM, 89% of initial activity | Sulfolobus acidocaldarius | |
EDTA | 1 mM, 93% of initial activity | Sulfolobus acidocaldarius | |
FeSO4 | 1 mM, 20% inhibition | Sulfolobus acidocaldarius | |
Hg(CH3COO)2 | 1 mM, 37% inhibition | Sulfolobus acidocaldarius | |
Hg2+ | 1 mM, 63% of initial activity | Sulfolobus acidocaldarius | |
iodoacetate | 1 mM, 12% inhibition | Sulfolobus acidocaldarius | |
additional information | the chlorides of Li+, Na+, K+, Ca2+, Mg2+, and Mn2+ do not affect the activity of the enzyme, whereas the sulfate of heavy metal ions such as Fe2+, Co2+, and Cu2+ cause a slight inactivation | Sulfolobus acidocaldarius | |
NaCl | 1 mM, 12% inhibition | Sulfolobus acidocaldarius | |
Zn2+ | 1 mM, 91% of initial activity | Sulfolobus acidocaldarius | |
ZnSO4 | 1 mM, 9% inhibition | Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
NADH | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
0.16 | - |
NADH | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
0.44 | - |
NAD+ | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
0.44 | - |
NAD+ | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
0.71 | - |
isatin | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
0.71 | - |
isatin | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
1.05 | - |
(S)-alpha-tetralol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
1.05 | - |
(S)-alpha-tetralol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
1.28 | - |
(R)-alpha-tetralol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
1.3 | - |
(R)-alpha-tetralol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
2.4 | - |
(R)-1-indanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
2.4 | - |
(R)-1-indanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
2.8 | - |
methyl o-chlorobenzoylformate | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
2.8 | - |
methyl o-chlorobenzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
4.2 | - |
cis-decahydro-1-naphthol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
5.3 | - |
ethyl benzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
6.4 | - |
(S)-1-indanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
6.4 | - |
(S)-1-indanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
6.5 | - |
methyl benzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
9.3 | - |
cycloheptanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
9.3 | - |
cycloheptanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
9.9 | - |
3-Methylcyclohexanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
12.1 | - |
1-phenyl-1,2-propanedione | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
23.5 | - |
2,2,2-trifluoroacetophenone | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
66 | - |
3-methylcyclohexanone | 65°C, pH 5.5 | Sulfolobus acidocaldarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | 1 mM, 106% of initial activity | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28978 | - |
4 * 28978, calculated from sequence | Sulfolobus acidocaldarius |
28978 | - |
4 * 28978, calculated, 4 * 30000, SDS-PAGE | Sulfolobus acidocaldarius |
30000 | - |
4 * 30000, SDS-PAGE | Sulfolobus acidocaldarius |
30000 | - |
4 * 28978, calculated, 4 * 30000, SDS-PAGE | Sulfolobus acidocaldarius |
65000 | - |
gel filtration | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sulfolobus acidocaldarius | the physiological direction of the catalytic reaction is reduction rather than oxidation | ? | - |
? | |
additional information | Sulfolobus acidocaldarius DSM 639 | the physiological direction of the catalytic reaction is reduction rather than oxidation | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
1,4-dioxane | 30%, 530% inactivation of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius |
1,4-dioxane | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius |
2-propanol | 120125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 530% of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius |
acetonitrile | 120125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 530% of the initial values following 5 h incubation at 50°C | Sulfolobus acidocaldarius |
dioxane | 17%, 160% of initial activity | Sulfolobus acidocaldarius |
Ethyl acetate | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius |
Ethyl acetate | 30%, 180% of initial activity | Sulfolobus acidocaldarius |
methyl-tert-butylether | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius |
n-heptane | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius |
n-heptane | 30%, 180% of initial activity | Sulfolobus acidocaldarius |
n-hexane | significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% | Sulfolobus acidocaldarius |
tert-butylmethyl ether | 30%, 210% of initial activity | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q4J702 | - |
- |
Sulfolobus acidocaldarius | Q4J9F2 | - |
- |
Sulfolobus acidocaldarius DSM 639 | Q4J702 | - |
- |
Sulfolobus acidocaldarius DSM 639 | Q4J9F2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.6 | - |
pH 5.5, 65°C | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1-indanol + NAD+ | 62% of activity compared to (S)-1-indanol | Sulfolobus acidocaldarius | ? | - |
? | |
(R)-1-indanol + NAD+ | - |
Sulfolobus acidocaldarius | 1-indanone + NADH + H+ | - |
r | |
(R)-alpha-tetralol + NAD+ | 58% of activity compared to (S)-1-indanol | Sulfolobus acidocaldarius | ? | - |
? | |
(R)-alpha-tetralol + NAD+ | - |
Sulfolobus acidocaldarius | alpha-tetralone + NADH + H+ | - |
r | |
(S)-1-indanol + NAD+ | - |
Sulfolobus acidocaldarius | ? | - |
? | |
(S)-1-indanol + NAD+ | - |
Sulfolobus acidocaldarius | 1-indanone + NADH + H+ | - |
r | |
(S)-2-pentanol + NAD+ | 11% of activity compared to (S)-1-indanol | Sulfolobus acidocaldarius | ? | - |
? | |
(S)-alpha-tetralol + NAD+ | - |
Sulfolobus acidocaldarius | alpha-tetralone + NADH + H+ | - |
r | |
(S)-alpha-tetralol + NAD+ | - |
Sulfolobus acidocaldarius DSM 639 | alpha-tetralone + NADH + H+ | - |
r | |
(S)-alpha-tetralol + NAD+ | 12% of activity compared to (S)-1-indanol | Sulfolobus acidocaldarius | ? | - |
? | |
1-indanone + NADH + H+ | - |
Sulfolobus acidocaldarius | (S)-1-indanol + NAD+ | 6 h, 50°C, 6% conversion, 25% enantiomeric excess | r | |
1-indanone + NADH + H+ | - |
Sulfolobus acidocaldarius DSM 639 | (S)-1-indanol + NAD+ | 6 h, 50°C, 6% conversion, 25% enantiomeric excess | r | |
1-phenyl-1,2-propanedione + NADH | about 25% of the activity compared to isatin | Sulfolobus acidocaldarius | ? | - |
? | |
2,2,2-trifluoroacetophenone + NADH | about 35% of the activity compared to isatin | Sulfolobus acidocaldarius | 2,2,2-trifluoro-1-phenylethanol + NAD+ | - |
? | |
3-methylcyclohexanol + NAD+ | 14% of activity compared to (S)-1-indanol | Sulfolobus acidocaldarius | 3-methylcyclohexanone + NADH + H+ | - |
? | |
3-methylcyclohexanone + NADH | about 10% of the activity compared to isatin | Sulfolobus acidocaldarius | 3-methylcyclohexanol + NAD+ | - |
? | |
3-methylcyclohexanone + NADH | about 10% of the activity compared to isatin | Sulfolobus acidocaldarius DSM 639 | 3-methylcyclohexanol + NAD+ | - |
? | |
cis-decahydro-1-naphthol + NAD+ | 11% of activity compared to (S)-1-indanol | Sulfolobus acidocaldarius | ? | - |
? | |
cycloheptanol + NAD+ | - |
Sulfolobus acidocaldarius | cycloheptanone + NADH + H+ | - |
r | |
cycloheptanol + NAD+ | 37% of activity compared to (S)-1-indanol | Sulfolobus acidocaldarius | cycloheptanone + NADH + H+ | - |
? | |
ethyl benzoylformate + NADH | about 30% of the activity compared to isatin | Sulfolobus acidocaldarius | ? | - |
? | |
ethyl benzoylformate + NADH + H+ | - |
Sulfolobus acidocaldarius | ethyl (R)-mandelate + NAD+ | 50% enantiomeric excess | r | |
ethyl benzoylformate + NADH + H+ | - |
Sulfolobus acidocaldarius DSM 639 | ethyl (R)-mandelate + NAD+ | 50% enantiomeric excess | r | |
ethyl pyruvate + NADH | about 15% of the activity compared to isatin | Sulfolobus acidocaldarius | ? | - |
? | |
ethyl pyruvate + NADH | about 15% of the activity compared to isatin | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
isatin + NADH | - |
Sulfolobus acidocaldarius | ? | - |
? | |
isatin + NADH + H+ | - |
Sulfolobus acidocaldarius | ? + NAD+ | - |
r | |
isatin + NADH + H+ | - |
Sulfolobus acidocaldarius DSM 639 | ? + NAD+ | - |
r | |
methyl benzoylformate + NADH | about 35% of the activity compared to isatin | Sulfolobus acidocaldarius | ? | - |
? | |
methyl benzoylformate + NADH | about 35% of the activity compared to isatin | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
methyl benzoylformate + NADH + H+ | - |
Sulfolobus acidocaldarius | methyl (S)-mandelate + NAD+ | 6 h, 100% conversion, 17% enantiomeric excess | r | |
methyl o-chlorobenzoylformate + NADH | about 55% of the activity compared to isatin | Sulfolobus acidocaldarius | ? | - |
? | |
methyl o-chlorobenzoylformate + NADH + H+ | - |
Sulfolobus acidocaldarius | methyl (R)-o-chloromandelate + NAD+ | 6 h, 99% conversion, 72% enantiomeric excess | r | |
additional information | the physiological direction of the catalytic reaction is reduction rather than oxidation | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | the physiological direction of the catalytic reaction is reduction rather than oxidation | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
additional information | the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
additional information | enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes | Sulfolobus acidocaldarius DSM 639 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | tetramer structure results from chemical crosslinking experiments | Sulfolobus acidocaldarius |
tetramer | 4 * 30000, SDS-PAGE | Sulfolobus acidocaldarius |
tetramer | 4 * 28978, calculated from sequence | Sulfolobus acidocaldarius |
tetramer | 4 * 28978, calculated, 4 * 30000, SDS-PAGE | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
SaADH | - |
Sulfolobus acidocaldarius |
Saci_1232 | - |
Sulfolobus acidocaldarius |
short-chain ADH | - |
Sulfolobus acidocaldarius |
short-chain NAD(H)-dependent dehydrogenase/reductase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Sulfolobus acidocaldarius |
75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | 65°C: about 40% of maximal activity, 85°C: about 35% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
24 h, 109% of initial activity | Sulfolobus acidocaldarius |
70 | - |
pH 9.0, 50 mM Tris-HCl, 24% loss of acrtivity | Sulfolobus acidocaldarius |
70 | - |
24 h, 76% of initial activity | Sulfolobus acidocaldarius |
90 | - |
half-life 30 min | Sulfolobus acidocaldarius |
90 | - |
half-inactivation temperature is 30 min | Sulfolobus acidocaldarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
cis-decahydro-1-naphthol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
2 | 8 | NADH | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
2 | 8 | NADH | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
2.1 | - |
(S)-alpha-tetralol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
2.1 | - |
(S)-alpha-tetralol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
2.35 | - |
ethyl benzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
3.1 | - |
1-phenyl-1,2-propanedione | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
3.7 | - |
NAD+ | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
3.7 | - |
NAD+ | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
3.8 | - |
3-methylcyclohexanone | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
4.4 | - |
cycloheptanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
4.4 | - |
cycloheptanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
6.2 | - |
methyl benzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
7.1 | - |
(R)-1-indanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
7.1 | - |
(R)-1-indanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
9.2 | - |
methyl o-chlorobenzoylformate | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
9.2 | - |
methyl o-chlorobenzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
10 | - |
2,2,2-trifluoroacetophenone | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
13.5 | - |
(R)-alpha-tetralol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
13.5 | - |
(R)-alpha-tetralol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
13.7 | - |
(S)-1-indanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
13.7 | - |
(S)-1-indanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
22 | - |
isatin | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
22 | - |
isatin | 65°C, pH 5.5 | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.1 | - |
reduction reaction | Sulfolobus acidocaldarius |
5.1 | - |
reduction of ketones | Sulfolobus acidocaldarius |
5.5 | - |
assay at | Sulfolobus acidocaldarius |
8.2 | - |
oxidation reaction | Sulfolobus acidocaldarius |
8.2 | - |
oxidation of alcohols | Sulfolobus acidocaldarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.9 | 5.8 | pH 4.9: about 25% of maximal activity, pH 5.8: about 40% of maximal activity, reduction reaction | Sulfolobus acidocaldarius |
6.7 | 8.5 | pH 6.7: about 40% of maximal activity, pH 8.5: about 85% of maximal activity, oxidation reaction | Sulfolobus acidocaldarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme transfers the deuteride to the Si-face of NAD+ | Sulfolobus acidocaldarius | |
NADH | strictly required | Sulfolobus acidocaldarius | |
NADH | the specificity constant value is 21-fold higher for NADH than NAD+. No activity with NADP(H) | Sulfolobus acidocaldarius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
3-methylcyclohexanone | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
0.23 | - |
cis-decahydro-1-naphthol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
0.25 | - |
1-phenyl-1,2-propanedione | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
0.42 | - |
2,2,2-trifluoroacetophenone | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
0.44 | - |
ethyl benzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
0.47 | - |
cycloheptanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
0.47 | - |
cycloheptanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
0.95 | - |
methyl benzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
2 | - |
(S)-alpha-tetralol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
2 | - |
(S)-alpha-tetralol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
2.14 | - |
(S)-1-indanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
2.14 | - |
(S)-1-indanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
2.98 | - |
(R)-1-indanol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
2.98 | - |
(R)-1-indanol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
3.3 | - |
methyl o-chlorobenzoylformate | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
3.3 | - |
methyl o-chlorobenzoylformate | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
8.4 | - |
NAD+ | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
8.4 | - |
NAD+ | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
10.5 | - |
(R)-alpha-tetralol | 65°C, pH 8.0 | Sulfolobus acidocaldarius | |
10.5 | - |
(R)-alpha-tetralol | pH 8.0, 65°C | Sulfolobus acidocaldarius | |
31 | - |
isatin | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
31 | - |
isatin | 65°C, pH 5.5 | Sulfolobus acidocaldarius | |
175 | - |
NADH | pH 5.5, 65°C | Sulfolobus acidocaldarius | |
175 | - |
NADH | 65°C, pH 5.5 | Sulfolobus acidocaldarius |