Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.1 extracted from

  • Palma-Gutierrez, H.; Rodriguez-Zavala, J.; Jasso-Chavez, R.; Moreno-Sanchez, R.; Saavedra, E.
    Gene cloning and biochemical characterization of an alcohol dehydrogenase from Euglena gracilis (2008), J. Eukaryot. Microbiol., 55, 554-561.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21DE3pLysS cells Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NAD+ at pH 9.0 in bis-Tris propane buffer Euglena gracilis
0.39
-
NAD+ at pH 7.0 and 25°C Euglena gracilis
3.1
-
ethanol at pH 9.0 in bis-Tris propane buffer Euglena gracilis
3.2
-
ethanol at pH 7.0 and 25°C Euglena gracilis
20.3
-
Isopropanol at pH 7.0 and 25°C Euglena gracilis
197
-
Isopentanol at pH 7.0 and 25°C Euglena gracilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essential for activity, in the absence of Mg2+ in the kinetic assay no ADH activity is detected Euglena gracilis
Zn2+ 20% of activity is obtained when replacing Mg2+ with 5 mM ZnSO4 Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
SDS-PAGE Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis B8QU18
-
-
Euglena gracilis Z B8QU18
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon Co2+-affinity column chromatography Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
-20°C, in the presence of 50% (v/v) glycerol and at highly concentrated solutions (0.75-1 mg protein/ml), 3 days, 40-50% loss of activity Euglena gracilis
4°C, in 3.2 M ammonium sulfate or 50% glycerol, 1 day, rapid loss of activity Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + NAD+
-
Euglena gracilis acetaldehyde + NADH + H+
-
?
ethanol + NAD+
-
Euglena gracilis Z acetaldehyde + NADH + H+
-
?
isopentanol + NAD+ low affinity Euglena gracilis ? + NADH + H+
-
?
isopentanol + NAD+ low affinity Euglena gracilis Z ? + NADH + H+
-
?
isopropanol + NAD+ low affinity Euglena gracilis acetone + NADH + H+
-
?
isopropanol + NAD+ low affinity Euglena gracilis Z acetone + NADH + H+
-
?
additional information no activity is detected using 1 mM NADP+ Euglena gracilis ?
-
?
additional information no activity is detected using 1 mM NADP+ Euglena gracilis Z ?
-
?

Synonyms

Synonyms Comment Organism
ADH
-
Euglena gracilis
medium-chain NAD+-dependent ADH
-
Euglena gracilis
NAD+-ADH
-
Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 for ethanol oxidation Euglena gracilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 with 100 mM diphosphate buffer at pH 9.0, the enzyme only exhibits 49% of activity, a 50% decrease is obtained using 50 mM bis-Tris propane buffer at pH 7.0-7.5 and 9.0, whereas at pH 8 and 8.5 only a 10% reduction is found Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for NAD+ Euglena gracilis