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Literature summary for 1.1.1.1 extracted from

  • Ammendola, S.; Raucci, G.; Incani, O.; Mele, A.; Tramontano, A.; Wallace, A.
    Replacing the glutamate ligand in the structural zinc site of Sulfolobus solfataricus alcohol dehydrogenase with a cysteine decreases thermostability. (1995), Protein Eng., 8, 31-37.
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109(DE3) cells Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
E97C shows the same activity but a reduced thermostability with respect to the wild type recombinant protein Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Toyopearl Butyl 650 S column chromatography, Q Sepharose fast flow column chromatography, Superose 12 prep-grade gel filtration, and TosoHaas G 2000 SWXL gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 wild type recombinant enzyme, from crude extract, at 65°C Saccharolobus solfataricus
0.02
-
 mutant recombinant enzyme E97C, from crude extract, at 65°C Saccharolobus solfataricus
4.5
-
 wild type recombinant enzyme, after 450fold purification, at 65°C Saccharolobus solfataricus
5.2
-
 mutant recombinant enzyme E97C, after 260fold purification, at 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl alcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSADH
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the activity of the recombinant wild type enzyme slowly decreases to 50% after 16 h at 65°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharolobus solfataricus