Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 68% of total activity measured | Pseudomonas putida | 5737 | - |
membrane | 32% of total activity measured | Pseudomonas putida | 16020 | - |
additional information | no activity can be localized in the periplasm | Pseudomonas putida | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56700 | - |
deduced from amino acid sequence | Pseudomonas putida |
57000 | - |
SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q76HN6 | probable alcohol dehydrogenase | - |
Pseudomonas putida S-5 | Q76HN6 | probable alcohol dehydrogenase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NAD+ | 30% activity compared to benzyl alcohol | Pseudomonas putida | butanal + NADH + H+ | - |
? | |
1-butanol + NAD+ | 30% activity compared to benzyl alcohol | Pseudomonas putida S-5 | butanal + NADH + H+ | - |
? | |
1-decanol + NAD+ | 13% activity compared to benzyl alcohol | Pseudomonas putida | decanal + NADH + H+ | - |
? | |
1-decanol + NAD+ | 13% activity compared to benzyl alcohol | Pseudomonas putida S-5 | decanal + NADH + H+ | - |
? | |
1-heptanol + NAD+ | 25% activity compared to benzyl alcohol | Pseudomonas putida | heptanal + NADH + H+ | - |
? | |
1-heptanol + NAD+ | 25% activity compared to benzyl alcohol | Pseudomonas putida S-5 | heptanal + NADH + H+ | - |
? | |
1-hexanol + NAD+ | 61% activity compared to benzyl alcohol | Pseudomonas putida | hexanal + NADH + H+ | - |
? | |
1-hexanol + NAD+ | 61% activity compared to benzyl alcohol | Pseudomonas putida S-5 | hexanal + NADH + H+ | - |
? | |
1-nonanol + NAD+ | 12% activity compared to benzyl alcohol | Pseudomonas putida | nonanal + NADH + H+ | - |
? | |
1-octanol + NAD+ | 11% activity compared to benzyl alcohol | Pseudomonas putida | octanal + NADH + H+ | - |
? | |
1-pentanol + NAD+ | 152% activity compared to benzyl alcohol | Pseudomonas putida | pentanal + NADH + H+ | - |
? | |
4-nitrobenzyl alcohol + NAD+ | 94% activity compared to benzyl alcohol | Pseudomonas putida | 4-nitrobenzaldehyde + NADH + H+ | - |
? | |
benzaldehyde + NADH + H+ | 3% activity compared to benzyl alcohol | Pseudomonas putida | benzyl alcohol + NAD+ | - |
r | |
benzyl alcohol + NAD+ | 100% activity | Pseudomonas putida | benzaldehyde + NADH + H+ | - |
r | |
choline + NAD+ | 3% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH + H+ | - |
? | |
decyl aldehyde + NADH + H+ | 21% activity compared to benzyl alcohol | Pseudomonas putida | 1-decanol + NAD+ | - |
? | |
heptaldehyde + NADH + H+ | 33% activity compared to benzyl alcohol | Pseudomonas putida | ? + NAD+ | - |
? | |
hexaldehyde + NADH + H+ | 7% activity compared to benzyl alcohol | Pseudomonas putida | 1-hexanol + NAD+ | - |
? | |
hydrocinnamaldehyde + NADH + H+ | 12% activity compared to benzyl alcohol | Pseudomonas putida | hydrocinnamyl alcohol + NAD+ | - |
r | |
hydrocinnamyl alcohol + NAD+ | 31% activity compared to benzyl alcohol | Pseudomonas putida | hydrocinnamaldehyde + NADH + H+ | - |
r | |
additional information | no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde | Pseudomonas putida | ? | - |
? | |
additional information | no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde | Pseudomonas putida S-5 | ? | - |
? | |
nonyl aldehyde + NADH + H+ | 25% activity compared to benzyl alcohol | Pseudomonas putida | 1-nonanol + NAD+ | - |
? | |
nonylphenol polyethoxylate 9 + NAD+ | 7% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
octyl aldehyde + NADH + H+ | 29% activity compared to benzyl alcohol | Pseudomonas putida | 1-octanol + NAD+ | - |
? | |
octylphenol polyethoxylate 2 + NAD+ | 59% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
octylphenol polyethoxylate 8 + NAD+ | 7% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
polyoxyethylene 8 decyl ether + NAD+ | 12% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
Triton X-100 + NAD+ | 7% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
Triton X-114 + NAD+ | 3% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
Triton X-165 + NAD+ | 24% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
Triton X-35 + NAD+ | 43% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
Triton X-405 + NAD+ | 19% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? | |
Triton X-45 + NAD+ | 7% activity compared to benzyl alcohol | Pseudomonas putida | ? + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH1 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |