Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli RB791 cells | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
N249Y | mutant shows increased thermal stability and increased catalytic activity compared to the wild type enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADH | wild type enzyme, at 65°C, in the presence of benzaldehyde | Saccharolobus solfataricus | |
0.024 | - |
4-methoxybenzaldehyde | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
0.03 | - |
benzaldehyde | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
0.03 | - |
Cyclohexanol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
0.21 | - |
NADH | mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde | Saccharolobus solfataricus | |
0.23 | - |
4-methoxybenzyl alcohol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
0.26 | - |
benzaldehyde | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
0.26 | - |
benzyl alcohol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
0.5 | - |
NAD+ | wild type enzyme, at 65°C, in the presence of benzyl alcohol | Saccharolobus solfataricus | |
0.6 | - |
1-propanol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
0.86 | - |
4-methoxybenzaldehyde | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
1.24 | - |
4-methoxybenzyl alcohol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
1.5 | - |
benzyl alcohol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
1.7 | - |
1-propanol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
1.9 | - |
Cyclohexanol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
12.4 | - |
NAD+ | mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37585 | - |
4 * 37585, electrospray mass spectrometry | Saccharolobus solfataricus |
150300 | - |
electrospray mass spectrometry | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose Fast Flow column chromatography and G75 gel filtration | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.1 | - |
wild type enzyme, at 65°C | Saccharolobus solfataricus |
26.1 | - |
mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-propanol + NAD+ | - |
Saccharolobus solfataricus | propanal + NADH + H+ | - |
? | |
4-methoxybenzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | 4-methoxybenzaldehyde + NADH + H+ | - |
r | |
benzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | benzaldehyde + NADH + H+ | - |
r | |
cyclohexanol + NAD+ | - |
Saccharolobus solfataricus | cyclohexanone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 37585, electrospray mass spectrometry | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | wild type and mutant enzyme N249Y are stable at 70°C, the mutant enzyme seems more thermoresistant than the wild type enzyme up to a temperature of 80°C, after which its activity decreases abruptly | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
Cyclohexanol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
2.6 | - |
1-propanol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
3.3 | - |
4-methoxybenzaldehyde | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
3.3 | - |
4-methoxybenzyl alcohol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
3.4 | - |
NAD+ | wild type enzyme, at 65°C, in the presence of benzyl alcohol | Saccharolobus solfataricus | |
3.6 | - |
benzyl alcohol | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
10.3 | - |
NADH | wild type enzyme, at 65°C, in the presence of benzaldehyde | Saccharolobus solfataricus | |
11.3 | - |
benzaldehyde | wild type enzyme, at 65°C | Saccharolobus solfataricus | |
15.9 | - |
Cyclohexanol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
16.6 | - |
1-propanol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
20.5 | - |
4-methoxybenzaldehyde | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
21.7 | - |
benzyl alcohol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
25.3 | - |
NADH | mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde | Saccharolobus solfataricus | |
25.5 | - |
benzaldehyde | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
25.5 | - |
4-methoxybenzyl alcohol | mutant enzyme N249Y, at 65°C | Saccharolobus solfataricus | |
50 | - |
NAD+ | mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.5 | apparent optimal pH for the benzaldehyde reduction | Saccharolobus solfataricus |
8.5 | - |
mutant enzyme N249Y | Saccharolobus solfataricus |
9 | - |
wild type enzyme | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent | Saccharolobus solfataricus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.7 | - |
wild type enzyme | Saccharolobus solfataricus | guanidine hydrochloride | |
2.2 | - |
mutant enzyme N249Y | Saccharolobus solfataricus | guanidine hydrochloride |