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Literature summary for 1.1.1.1 extracted from

  • Giordano, A.; Cannio, R.; La Cara, F.; Bartolucci, S.; Rossi, M.; Raia, C.A.
    Asn249Tyr substitution at the coenzyme binding domain activates Sulfolobus solfataricus alcohol dehydrogenase and increases its thermal stability. (1999), Biochemistry, 38, 3043-3054.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli RB791 cells Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
N249Y mutant shows increased thermal stability and increased catalytic activity compared to the wild type enzyme Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
NADH wild type enzyme, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
0.024
-
4-methoxybenzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
0.03
-
benzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
0.03
-
Cyclohexanol wild type enzyme, at 65°C Saccharolobus solfataricus
0.21
-
NADH mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
0.23
-
4-methoxybenzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
0.26
-
benzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
0.26
-
benzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
0.5
-
NAD+ wild type enzyme, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus
0.6
-
1-propanol wild type enzyme, at 65°C Saccharolobus solfataricus
0.86
-
4-methoxybenzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.24
-
4-methoxybenzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.5
-
benzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.7
-
1-propanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.9
-
Cyclohexanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
12.4
-
NAD+ mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37585
-
4 * 37585, electrospray mass spectrometry Saccharolobus solfataricus
150300
-
electrospray mass spectrometry Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose Fast Flow column chromatography and G75 gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
wild type enzyme, at 65°C Saccharolobus solfataricus
26.1
-
mutant enzyme N249Y, at 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-propanol + NAD+
-
Saccharolobus solfataricus propanal + NADH + H+
-
?
4-methoxybenzyl alcohol + NAD+
-
Saccharolobus solfataricus 4-methoxybenzaldehyde + NADH + H+
-
r
benzyl alcohol + NAD+
-
Saccharolobus solfataricus benzaldehyde + NADH + H+
-
r
cyclohexanol + NAD+
-
Saccharolobus solfataricus cyclohexanone + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 37585, electrospray mass spectrometry Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
ADH
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 wild type and mutant enzyme N249Y are stable at 70°C, the mutant enzyme seems more thermoresistant than the wild type enzyme up to a temperature of 80°C, after which its activity decreases abruptly Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
Cyclohexanol wild type enzyme, at 65°C Saccharolobus solfataricus
2.6
-
1-propanol wild type enzyme, at 65°C Saccharolobus solfataricus
3.3
-
4-methoxybenzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
3.3
-
4-methoxybenzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
3.4
-
NAD+ wild type enzyme, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus
3.6
-
benzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
10.3
-
NADH wild type enzyme, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
11.3
-
benzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
15.9
-
Cyclohexanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
16.6
-
1-propanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
20.5
-
4-methoxybenzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
21.7
-
benzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
25.3
-
NADH mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
25.5
-
benzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
25.5
-
4-methoxybenzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
50
-
NAD+ mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.5 apparent optimal pH for the benzaldehyde reduction Saccharolobus solfataricus
8.5
-
mutant enzyme N249Y Saccharolobus solfataricus
9
-
wild type enzyme Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent Saccharolobus solfataricus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.7
-
wild type enzyme Saccharolobus solfataricus guanidine hydrochloride
2.2
-
mutant enzyme N249Y Saccharolobus solfataricus guanidine hydrochloride