Protein Variants | Comment | Organism |
---|---|---|
P47A | site-directed mutagenesis, about 100fold increased activity compared to the wild-type enzyme | Mus musculus |
P47H | site-directed mutagenesis, about 100fold increased activity compared to the wild-type enzyme | Mus musculus |
P47Q | site-directed mutagenesis, about 100fold increased activity compared to the wild-type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclohexylformamide | dead-end inhibition pattern | Mus musculus | |
octanoic acid | dead-end inhibition pattern | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | catalytic zinc ion | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9QYY9 | low-activity isozyme ADH2 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a primary alcohol + NAD+ = an aldehyde + NADH + H+ | ordered bibi mechanism, structural and functional implications of amino acid residue 47 | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | - |
Mus musculus | benzaldehyde + NADH | - |
r | |
octanol + NAD+ | - |
Mus musculus | octanal + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH2 | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is strongly pH-dependent | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus | |
NADH | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
octanoic acid | pH 7.5, 25°C, wild-type enzyme, versus NAD+ | Mus musculus | |
0.035 | - |
cyclohexylformamide | pH 7.5, 25°C, wild-type enzyme, versus benzaldehyde | Mus musculus | |
0.037 | - |
octanoic acid | pH 7.5, 25°C, wild-type enzyme, versus octanol | Mus musculus | |
0.072 | - |
cyclohexylformamide | pH 7.5, 25°C, wild-type enzyme, versus NADH | Mus musculus |