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Literature summary for 1.1.1.1 extracted from

  • Hensgens, C.M.H.; Vonck, J.; van Beeumen, J.; Bruggen, E.F.J.; Hansen, T.A.
    Purification and characterization of an oxygen-labile, NAD-dependent alcohol dehydrogenase from Desulfovibrio gigas (1993), J. Bacteriol., 175, 2859-2863.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
 Megalodesulfovibrio gigas
Co2+
-
 Megalodesulfovibrio gigas
EDTA
-
 Megalodesulfovibrio gigas
Mn2+
-
 Megalodesulfovibrio gigas
NEM
-
 Megalodesulfovibrio gigas
Ni2+
-
 Megalodesulfovibrio gigas
p-chloromercuribenzene sulfonate
-
 Megalodesulfovibrio gigas
PCMB
-
 Megalodesulfovibrio gigas
Zn2+
-
 Megalodesulfovibrio gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADH reduction of acetaldehyde Megalodesulfovibrio gigas
0.074
-
 NAD+ oxidation of ethanol Megalodesulfovibrio gigas
0.15
-
 ethanol
-
 Megalodesulfovibrio gigas
0.28
-
 propan-1-ol
-
 Megalodesulfovibrio gigas
1.8
-
 acetaldehyde
-
 Megalodesulfovibrio gigas
50
-
 formaldehyde
-
 Megalodesulfovibrio gigas

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc contains 1.2 Zn atom per subunit Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 10 * 43000, SDS-PAGE Megalodesulfovibrio gigas
320000
-
 gel filtration Megalodesulfovibrio gigas

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas
-
-
-

Oxidation Stability

Oxidation Stability Organism
pronounced oxygen lability at pH 9, even at pH 7, 2 h under air, 80% loss of activity Megalodesulfovibrio gigas

Purification (Commentary)

Purification (Comment) Organism
-
 Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.8
-
-
 Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propanediol + NAD+ 7% of the activity with ethanol Megalodesulfovibrio gigas ? + NADH
-
 ?
acetaldehyde + NADH + H+ the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol Megalodesulfovibrio gigas ethanol + NAD+
-
 r
butyraldehyde + NADH
-
 Megalodesulfovibrio gigas n-butanol + NAD+
-
 r
ethanol + NAD+ the reduction of acetaldehyde is 4.9fold faster than the oxidation of ethanol Megalodesulfovibrio gigas acetaldehyde + NADH
-
 r
formaldehyde + NADH + H+
-
 Megalodesulfovibrio gigas methanol + NAD+
-
 ?
propan-1,2-diol + NAD+
-
 Megalodesulfovibrio gigas ? + NADH
-
 ?
propan-2-ol + NAD+ 3% of the activity with ethanol Megalodesulfovibrio gigas acetone + NADH
-
 ?
propanol + NAD+
-
 Megalodesulfovibrio gigas propionaldehyde + NADH + H+
-
 ?
propionaldehyde + NADH
-
 Megalodesulfovibrio gigas propanol + NAD+
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 43000, SDS-PAGE Megalodesulfovibrio gigas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Megalodesulfovibrio gigas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 reduction of acetaldehyde Megalodesulfovibrio gigas
9.5
-
 oxidation of ethanol Megalodesulfovibrio gigas

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Megalodesulfovibrio gigas
NADH
-
 Megalodesulfovibrio gigas
NADP+ at 1.5% of the activity with NAD+ Megalodesulfovibrio gigas
NADPH at 1.6% of the activity with NADH Megalodesulfovibrio gigas