KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
NAD+ | wild-type enzyme ADH1-1S | Zea mays | |
0.038 | - |
NAD+ | mutant enzyme ADH1-1S1108 | Zea mays | |
0.042 | - |
NADH | mutant enzyme ADH1-1S1108 | Zea mays | |
0.085 | - |
NADH | wild-type enzyme ADH1-1S | Zea mays | |
22 | - |
ethanol | mutant enzyme ADH1S-1108 | Zea mays | |
24 | - |
ethanol | wild-type enzyme ADH1-1S | Zea mays | |
70 | - |
acetaldehyde | mutant enzyme ADH1-1S1108 | Zea mays | |
85 | - |
acetaldehyde | wild-type enzyme ADH1-1S | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
wild-type enzyme Adh1-1S and mutant enzyme Adh1-1S1108 | - |
Purification (Comment) | Organism |
---|---|
wild-type enzyme Adh1-1S and mutant enzyme Adh1-1S1108 | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kernel | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65 | - |
mutant enzyme Adh1-1S1108 | Zea mays |
70 | - |
wild-type enzyme Adh1-1S | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylpropan-1-ol + NAD+ | - |
Zea mays | 2-methyl-propan-1-one + NADH | - |
? | |
allyl alcohol + NAD+ | - |
Zea mays | prop-2-en-1-al + NADH | - |
? | |
butan-2-ol + NAD+ | no activity | Zea mays | butan-2-one + NADH | - |
? | |
butanol + NAD+ | - |
Zea mays | butyraldehyde + NADH | - |
? | |
ethanol + NAD+ | - |
Zea mays | acetaldehyde + NADH | - |
? | |
hexan-1-ol + NAD+ | - |
Zea mays | n-hexanal + NADH | - |
? | |
propan-2-ol + NAD+ | - |
Zea mays | acetone + NADH | - |
? | |
propanol + NAD+ | - |
Zea mays | propionaldehyde + NADH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
wild-type enzyme Adh1-1S is more stable than mutant enzyme Adh1-1S1108 | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
mutant enzyme ADH1-1S1108 | Zea mays |
8.5 | - |
wild-type enzyme ADH1-1S | Zea mays |
9 | - |
oxidation of ethanol | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zea mays | |
NADH | - |
Zea mays |