KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
acetaldehyde | - |
Sporotrichum pulverulentum | |
0.021 | - |
NADH | - |
Sporotrichum pulverulentum | |
0.17 | - |
NAD+ | - |
Sporotrichum pulverulentum | |
2.5 | - |
ethanol | - |
Sporotrichum pulverulentum | |
64 | - |
ethanol | - |
Sporotrichum pulverulentum | |
64 | - |
propan-2-ol | - |
Sporotrichum pulverulentum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Sporotrichum pulverulentum |
176000 | - |
non-denaturing PAGE | Sporotrichum pulverulentum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporotrichum pulverulentum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sporotrichum pulverulentum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
196.3 | - |
- |
Sporotrichum pulverulentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ethylhexan-1-ol + NAD+ | weak activity | Sporotrichum pulverulentum | 2-ethylhexanal + NADH | - |
? | |
2-methylpropan-1-ol + NAD+ | - |
Sporotrichum pulverulentum | 2-methyl-propan-1-one + NADH | - |
? | |
acetaldehyde + NADH + H+ | - |
Sporotrichum pulverulentum | ethanol + NAD+ | - |
r | |
butan-2-ol + NAD+ | - |
Sporotrichum pulverulentum | butan-2-one + NADH | - |
? | |
ethanol + NAD+ | - |
Sporotrichum pulverulentum | acetaldehyde + NADH | - |
r | |
hexanol + NAD+ | - |
Sporotrichum pulverulentum | n-hexanal + NADH | - |
? | |
methanol + NAD+ | no activity | Sporotrichum pulverulentum | formaldehyde + NADH + H+ | - |
? | |
octan-1-ol + NAD+ | weak activity | Sporotrichum pulverulentum | n-octanal + NADH | - |
? | |
octan-2-ol + NAD+ | weak activity | Sporotrichum pulverulentum | octan-2-one + NADH | - |
? | |
pentan-2-ol + NAD+ | - |
Sporotrichum pulverulentum | 2-pentanone + NADH | - |
? | |
pentanal + NAD+ | - |
Sporotrichum pulverulentum | pentanone + NADH | - |
? | |
propan-2-ol + NAD+ | - |
Sporotrichum pulverulentum | acetone + NADH | - |
? | |
propanol + NAD+ | - |
Sporotrichum pulverulentum | propionaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Sporotrichum pulverulentum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Sporotrichum pulverulentum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | 10°C: about 65% of maximal activity, 50°C: about 40% of maximal activity | Sporotrichum pulverulentum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | 6.5 | oxidation of ethanol | Sporotrichum pulverulentum |
8 | 8.4 | reduction of aldehyde | Sporotrichum pulverulentum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 6.8 | pH 5.2: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, oxidation of ethanol | Sporotrichum pulverulentum |
7.2 | 9.2 | pH 7.2: about 50% of maximal activity, pH 9.2: about 60% of maximal activity | Sporotrichum pulverulentum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sporotrichum pulverulentum | |
NADH | - |
Sporotrichum pulverulentum |