Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Zea mays | |
Cu2+ | no effect | Zea mays | |
Hg2+ | no effect | Zea mays | |
iodoacetamide | - |
Zea mays | |
NEM | - |
Zea mays | |
p-hydroxymercuribenzoate | - |
Zea mays | |
PCMB | - |
Zea mays | |
PMSF | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | required for activity, tightly bound within the enzyme | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Zea mays |
70000 | - |
gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
3 non-allelic alcohol dehydrogenase isoenzymes | - |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
scutellum | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Zea mays |
Storage Stability | Organism |
---|---|
-20°C, isoenzyme 2 is stable in 50% ethylene glycol for at least 3 months, isoenzyme 1 and 3 maintain less than 50% of their original activity after 2 weeks | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-butene-1-ol + NAD+ | - |
Zea mays | ? + NADH | - |
? | |
2-methylpropan-1-ol + NAD+ | weak activity | Zea mays | 2-methyl-propan-1-one + NADH | - |
? | |
3-butene-1-ol + NAD+ | - |
Zea mays | ? + NADH | - |
? | |
3-phenyl-2-propen-1-ol + NAD+ | - |
Zea mays | ? + NADH | - |
? | |
allyl alcohol + NAD+ | - |
Zea mays | prop-2-en-1-al + NADH | - |
? | |
benzyl alcohol + NAD+ | no activity | Zea mays | benzaldehyde + NADH | - |
? | |
butan-2-ol + NAD+ | weak activity | Zea mays | butan-2-one + NADH | - |
? | |
butanol + NAD+ | - |
Zea mays | butyraldehyde + NADH | - |
? | |
cyclohexanol + NAD+ | no activity | Zea mays | cyclohexanone + NADH | - |
? | |
ethanol + NAD+ | - |
Zea mays | acetaldehyde + NADH | - |
? | |
hexan-1-ol + NAD+ | - |
Zea mays | n-hexanal + NADH | - |
? | |
methanol + NAD+ | no activity | Zea mays | formaldehyde + NADH + H+ | - |
? | |
octan-1-ol + NAD+ | - |
Zea mays | n-octanal + NADH | - |
? | |
pentanol + NAD+ | - |
Zea mays | n-pentanal + NADH | - |
? | |
propanol + NAD+ | - |
Zea mays | propionaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
60 min, 73% loss of activity of enzyme 1, 10% loss of activity of enzyme 2, 21% loss of activity of enzyme 3 | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Zea mays |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | isoenzyme 2 and isoenzyme 3 | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zea mays | |
NADH | - |
Zea mays |