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Literature summary for 1.1.1.1 extracted from

  • Algar, E.M.; Seeley, T.L.; Holmes, R.S.
    Purification and molecular properties of mouse alcohol dehydrogenase isozymes (1983), Eur. J. Biochem., 137, 139-147.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inhibition of isoenzyme A2 and C2, no inhibition of isoenzyme B2 Mus musculus
pyrazole inhibition of isoenzyme A2 and C2. Isoenzyme B2 is insensitive to pyrazole inhibition with trans-2-hexen-1-ol as substrate Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc isoenzyme A2 contains 2.7 mol of zinc per mol of enzyme, isoenzyme b2 contains 1.9 mol of zinc per mol of enzyme, isoenzyme C2 contains 3.2 mol of zinc per mol of enzyme. A and C subunits each contain two atoms of zinc, with at least one being involved catalytically, the b subunit probably contains a single non-catalytic zinc atom Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, isoenzyme B2, SDS-PAGE Mus musculus
43000
-
2 * 43000, isoenzyme A2, SDS-PAGE Mus musculus
47000
-
2 * 47000, isoenzyme C2, SDS-PAGE Mus musculus
79000
-
isoenzyme B2, gel filtration Mus musculus
83000
-
isoenzyme A2, gel filtration Mus musculus
85000
-
isoenzyme C2, gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + NAD+ Mus musculus role of the major liver isoenzyme A2 in ethanol metabolism acetaldehyde + NADH
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
isoenzyme A2, B2 and C2
-

Purification (Commentary)

Purification (Comment) Organism
liver isoenzyme A2 and B2 and stomach isoenzyme C2 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver isoenzyme A2 and B2 Mus musculus
-
stomach isoenzyme C2 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.36
-
isoenzyme A2 Mus musculus
8.08
-
isoenzyme B2 Mus musculus
64
-
isoenzyme C2 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-hex-2-en-1-ol + NAD+
-
Mus musculus (E)-hex-2-en-1-one + NADH
-
?
acetaldehyde + NADH + H+ reduced by isoenzyme A2 and C2, no activity with isoenzyme B2 Mus musculus ethanol + NAD+
-
?
benzaldehyde + NADH reduced by isoenzyme A2 and C2, no activity with isoenzyme B2 Mus musculus benzyl alcohol + NAD+
-
r
benzyl alcohol + NAD+ oxidized by isoenzyme A2 and C2 no activity with isoenzyme B2 Mus musculus benzaldehyde + NADH
-
?
cyclohexanol + NAD+ oxidized by isoenzyme A2, no activity with isoenzymes B2 and C2 Mus musculus cyclohexanone + NADH
-
?
ethanol + NAD+ no activity with isoenzyme B2, oxidized by isoenzyme A2 and C2 Mus musculus acetaldehyde + NADH
-
?
ethanol + NAD+ role of the major liver isoenzyme A2 in ethanol metabolism Mus musculus acetaldehyde + NADH
-
?
ethylene glycol + NAD+ oxidized by isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus ? + NADH
-
?
formaldehyde + NADH + H+ reduced by isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus methanol + NAD+
-
r
hexanol + NAD+
-
Mus musculus n-hexanal + NADH
-
?
methanol + NAD+ weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus formaldehyde + NADH + H+
-
?
methanol + NAD+ weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2 Mus musculus formaldehyde + NADH + H+
-
r
octan-1-ol + NAD+
-
Mus musculus n-octanal + NADH
-
?
p-nitrobenzaldehyde + NADH
-
Mus musculus p-nitrobenzyl alcohol + NAD+
-
?
pentanal + NAD+ oxidized by isoenzyme A2 and B2, no activity with isoenzyme C2 Mus musculus pentanone + NADH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, isoenzyme C2, SDS-PAGE Mus musculus
dimer 2 * 39000, isoenzyme B2, SDS-PAGE Mus musculus
dimer 2 * 43000, isoenzyme A2, SDS-PAGE Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mus musculus
NADH
-
Mus musculus