Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibition of isoenzyme A2 and C2, no inhibition of isoenzyme B2 | Mus musculus | |
pyrazole | inhibition of isoenzyme A2 and C2. Isoenzyme B2 is insensitive to pyrazole inhibition with trans-2-hexen-1-ol as substrate | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | isoenzyme A2 contains 2.7 mol of zinc per mol of enzyme, isoenzyme b2 contains 1.9 mol of zinc per mol of enzyme, isoenzyme C2 contains 3.2 mol of zinc per mol of enzyme. A and C subunits each contain two atoms of zinc, with at least one being involved catalytically, the b subunit probably contains a single non-catalytic zinc atom | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, isoenzyme B2, SDS-PAGE | Mus musculus |
43000 | - |
2 * 43000, isoenzyme A2, SDS-PAGE | Mus musculus |
47000 | - |
2 * 47000, isoenzyme C2, SDS-PAGE | Mus musculus |
79000 | - |
isoenzyme B2, gel filtration | Mus musculus |
83000 | - |
isoenzyme A2, gel filtration | Mus musculus |
85000 | - |
isoenzyme C2, gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + NAD+ | Mus musculus | role of the major liver isoenzyme A2 in ethanol metabolism | acetaldehyde + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isoenzyme A2, B2 and C2 | - |
Purification (Comment) | Organism |
---|---|
liver isoenzyme A2 and B2 and stomach isoenzyme C2 | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | isoenzyme A2 and B2 | Mus musculus | - |
stomach | isoenzyme C2 | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.36 | - |
isoenzyme A2 | Mus musculus |
8.08 | - |
isoenzyme B2 | Mus musculus |
64 | - |
isoenzyme C2 | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-hex-2-en-1-ol + NAD+ | - |
Mus musculus | (E)-hex-2-en-1-one + NADH | - |
? | |
acetaldehyde + NADH + H+ | reduced by isoenzyme A2 and C2, no activity with isoenzyme B2 | Mus musculus | ethanol + NAD+ | - |
? | |
benzaldehyde + NADH | reduced by isoenzyme A2 and C2, no activity with isoenzyme B2 | Mus musculus | benzyl alcohol + NAD+ | - |
r | |
benzyl alcohol + NAD+ | oxidized by isoenzyme A2 and C2 no activity with isoenzyme B2 | Mus musculus | benzaldehyde + NADH | - |
? | |
cyclohexanol + NAD+ | oxidized by isoenzyme A2, no activity with isoenzymes B2 and C2 | Mus musculus | cyclohexanone + NADH | - |
? | |
ethanol + NAD+ | no activity with isoenzyme B2, oxidized by isoenzyme A2 and C2 | Mus musculus | acetaldehyde + NADH | - |
? | |
ethanol + NAD+ | role of the major liver isoenzyme A2 in ethanol metabolism | Mus musculus | acetaldehyde + NADH | - |
? | |
ethylene glycol + NAD+ | oxidized by isoenzyme A2, no activity with isoenzyme B2 and C2 | Mus musculus | ? + NADH | - |
? | |
formaldehyde + NADH + H+ | reduced by isoenzyme A2, no activity with isoenzyme B2 and C2 | Mus musculus | methanol + NAD+ | - |
r | |
hexanol + NAD+ | - |
Mus musculus | n-hexanal + NADH | - |
? | |
methanol + NAD+ | weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2 | Mus musculus | formaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | weak activity with isoenzyme A2, no activity with isoenzyme B2 and C2 | Mus musculus | formaldehyde + NADH + H+ | - |
r | |
octan-1-ol + NAD+ | - |
Mus musculus | n-octanal + NADH | - |
? | |
p-nitrobenzaldehyde + NADH | - |
Mus musculus | p-nitrobenzyl alcohol + NAD+ | - |
? | |
pentanal + NAD+ | oxidized by isoenzyme A2 and B2, no activity with isoenzyme C2 | Mus musculus | pentanone + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, isoenzyme C2, SDS-PAGE | Mus musculus |
dimer | 2 * 39000, isoenzyme B2, SDS-PAGE | Mus musculus |
dimer | 2 * 43000, isoenzyme A2, SDS-PAGE | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus | |
NADH | - |
Mus musculus |