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Literature summary for 1.1.1.1 extracted from
- Rabbit liver alcohol dehydrogenase: purification and properties (1985), J. Biochem., 97, 1163-1172.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against 50 mM Tris-HCl buffer, stable after 5 h, 3% loss of activity after 1 day, 82% loss of activity after 6 days | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Oryctolagus cuniculus |  |
| 2,2'-dipyridyl | - |
Oryctolagus cuniculus | |
| 4-methoxypyrazole | - |
Oryctolagus cuniculus | |
| Ag+ | - |
Oryctolagus cuniculus | |
| Cu2+ | - |
Oryctolagus cuniculus | |
| glutathione | - |
Oryctolagus cuniculus | |
| Hg2+ | - |
Oryctolagus cuniculus | |
| iodoacetic acid | - |
Oryctolagus cuniculus | |
| NEM | - |
Oryctolagus cuniculus | |
| PCMB | - |
Oryctolagus cuniculus | |
| pyrazole | - |
Oryctolagus cuniculus | |
| Zn2+ | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00694 | - |
NAD+ | pH 7.5 | Oryctolagus cuniculus | |
| 0.0531 | - |
NAD+ | pH 10.8 | Oryctolagus cuniculus | |
| 0.45 | - |
ethanol | pH 10.8 | Oryctolagus cuniculus | |
| 3.33 | - |
ethanol | pH 7.5 | Oryctolagus cuniculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | contains 3.8 mol of Zn per mol of protein | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, SDS-PAGE | Oryctolagus cuniculus |
| 72000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.69 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in presence of 1.0 mM dithiothreitol, stable for 10 days | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butanol + NAD+ | - |
Oryctolagus cuniculus | butyraldehyde + NADH | - |
? | |
| ethanol + NAD+ | - |
Oryctolagus cuniculus | acetaldehyde + NADH | - |
? | |
| methanol + NAD+ | no activity at pH 7.5, slight activity at pH 10.8 | Oryctolagus cuniculus | formaldehyde + NADH + H+ | - |
? | |
| pentanol + NAD+ | - |
Oryctolagus cuniculus | n-pentanal + NADH | - |
? | |
| propan-2-ol + NAD+ | - |
Oryctolagus cuniculus | acetone + NADH | - |
? | |
| propanol + NAD+ | - |
Oryctolagus cuniculus | propionaldehyde + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.8 | - |
and a second lower maximum at pH 7.5 | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Oryctolagus cuniculus | |
| NADH | - |
Oryctolagus cuniculus | |
| NADP+ | no activity as coenzyme | Oryctolagus cuniculus | |
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