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Information on EC 6.5.1.5 - RNA 3'-terminal-phosphate cyclase (GTP)

for references in articles please use BRENDA:EC6.5.1.5
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EC Tree
IUBMB Comments
The enzyme, which is specific for GTP, was characterized from the archaeon Pyrococcus furiosus. The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in a GTP-dependent reaction. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by GTP, forming a phosphoramide bond between guanylate and a histidine residue. The guanylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]-3'-(5'-diphosphoguanosine). Finally, the enzyme catalyses an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the guanylate. cf. EC 6.5.1.4, RNA-3'-phosphate cyclase (ATP).
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
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GTP
+
[RNA]-3'-(3'-phospho-ribonucleoside)
=
GMP
+
diphosphate
+
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
+
[RNA]-3'-(3'-phospho-ribonucleoside)
=
+
+
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
Synonyms
pf-rtc, pf1549, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside) = [RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine)
show the reaction diagram
(1b)
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-
-
GTP + [RNA 3'-phosphate cyclase]-L-histidine = 5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine + diphosphate
show the reaction diagram
(1a)
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-
-
GTP + [RNA]-3'-(3'-phospho-ribonucleoside) = GMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside
show the reaction diagram
overall reaction
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-
-
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine) = [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + GMP
show the reaction diagram
(1c)
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-
-
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