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nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
additional information
?
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the pyridine N and carboxyl groups are important for interaction with the enzyme
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
in the presence of Mg2+ and inorganic phosphate
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
lack of the de novo pathway for NAD+ synthesis from tryptophan, utilizes both nicotinic acid and nicotinamide as NAD+ precursors
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
NAPRT is essential for nicotinic acid to increase cellular NAD levels and thus to prevent oxidative stress of the cells
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
+ whole cell lysates, pH 7.5, 37°C, 10 mM MgCl2, 2.5 mM dithiothreitol, 1 mM ATP, 25 microgram bovine serum albumin, with or without NAD+
termination by boiling for 60 seconds, analysis by thin layer chromatography (radiolabeled nicotinic acid) or ESI-MS, no NaMN detectable in Hep-G2 cells
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
essential for the NAD salvage pathway
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
mutants in the His-219 residue do catalyze the slow formation of nicotinic acid mononucleotide in the absence of ATP similarly to the wild type enzymes
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
no phosphoribosylation of nicotinamide, quinolic acid, adenine or hypoxanthine
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
5-phospho-alpha-D-ribose 1-diphosphate in complex with Mg
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
?
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
?
additional information
?
-
nicotinamide is not substrate
-
-
?
additional information
?
-
-
nicotinamide is not substrate
-
-
?
additional information
?
-
quinolinic acid is not substrate
-
-
?
additional information
?
-
-
quinolinic acid is not substrate
-
-
?
additional information
?
-
-
enzyme is able to generate adenosine 5'-tetraphosphate using substrate ATP and product phosphate, reaction of EC 3.6.1.14. Accompanying a typically irreversible hydrolysis of ATP to ADP and phosphate, is a fully reversible phosphate transfer reaction between ATP and adenosine 5'-tetraphosphate
-
-
?
additional information
?
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
ATP and phosphoribose diphosphate compete for ATP-binding site
-
-
?
additional information
?
-
-
ATPase activity in the presence of either product and in the absence of phosphoribose diphosphate
-
-
?
additional information
?
-
molecular evolution mechanism
-
-
?
additional information
?
-
-
molecular evolution mechanism
-
-
?
additional information
?
-
-
negligible ATPase activity in the absence of nicotinic acid
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
additional information
?
-
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
lack of the de novo pathway for NAD+ synthesis from tryptophan, utilizes both nicotinic acid and nicotinamide as NAD+ precursors
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
NAPRT is essential for nicotinic acid to increase cellular NAD levels and thus to prevent oxidative stress of the cells
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
essential for the NAD salvage pathway
-
-
r
additional information
?
-
molecular evolution mechanism
-
-
?
additional information
?
-
-
molecular evolution mechanism
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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2-hydroxynicotinic acid
-
3-Pyridylcarbinol
-
weak inhibitor
3-Pyridylcarboxaldehyde
-
-
5-Fluoronicotinic acid
-
-
5-phospho-alpha-D-ribose 1-diphosphate
acetyl-CoA
at 1 mM 40% inhibition of activity (concentrations of substrates 1 mM)
CoA
at 1 mM 70% inhibition of activity (concentrations of substrates 1 mM)
D-fructose 1,6-bisphosphate
at 1 mM 36% inhibition of activity (concentrations of substrates 1 mM)
glutaryl-CoA
at 1 mM 42% inhibition of activity (concentrations of substrates 1 mM)
glyceraldehyde 3-phosphate
at 1 mM 27% inhibition of activity (concentrations of substrates 1 mM)
Methyl 3-pyridylcarbinol
-
weak inhibitor
MgADP-
-
non-competitive to Mg-ATP or Mg-diphosphate
Monoiodoacetic acid
-
1 mM and above
N-ethylmaleimide
-
0.1 mM and above
nicotinate mononucleotide
nucleoside diphosphates
-
e.g. ADP or GDP
phosphate
-
0.1 M and above
phosphoenolpyruvate
at 1 mM 33% inhibition of activity (concentrations of substrates 1 mM)
Pyrazine 2-carboxylic acid
-
-
Pyridazine-4,5-dicarboxylic acid
-
weak, 10% less activity
succinyl-CoA
at 1 mM 53% inhibition of activity (concentrations of substrates 1 mM)
Urea
-
competitive to nicotinate mononucleotide
5-phospho-alpha-D-ribose 1-diphosphate
-
at high concentrations
5-phospho-alpha-D-ribose 1-diphosphate
-
at high concentrations, when Mg2+ below 0.03 mM
ATP
at high substrate saturation
ATP
-
in the presence of Mn2+
ATP
-
at high concentrations with Mg2+ below 0.03 mM
ATP
-
3 mM, competitive inhibition of nicotinic acid mononucleotide formation in mutants enzymes
ATP
-
in the presence of Mn2+
Mg2+
-
at higher concentrations than ATP, when free Mg2+ ions occur
Mg2+
-
above 10 mM, significant pH alterations
NAD+
-
not
NADP+
-
not
Nicotinate analogues
-
-
-
Nicotinate analogues
-
not
-
nicotinate mononucleotide
-
not
nicotinate mononucleotide
-
competitive to both substrates
nicotinate mononucleotide
-
-
nicotinate mononucleotide
-
competitive to MgATP2-; non-competitive to nicotinate
nicotinate mononucleotide
-
competitive to nicotinate and noncompetitive to phosphoribobose diphosphate
additional information
-
no inhibition by nicotinamide; no inhibition by quinolinic acid, AMP, GMP
-
additional information
-
no inhibition by nicotinamide; no inhibition by nicotinate, up to 10 mM
-
additional information
-
no inhibition by 4-hydroxy-nicotinate
-
additional information
not inhibited by up to 1 mM NAD+, lack of feedback inhibition by NAD+
-
additional information
-
not inhibited by up to 1 mM NAD+, lack of feedback inhibition by NAD+
-
additional information
not inhibitory: FK866. NaPRTase acks a tunnel that, in nicotinamide phosphoribosiltransferase EC 2.4.2.12, represents the binding site of inhibitor FK866
-
additional information
-
not inhibitory: FK866. NaPRTase acks a tunnel that, in nicotinamide phosphoribosiltransferase EC 2.4.2.12, represents the binding site of inhibitor FK866
-
additional information
-
no inhibition by antibodies against pig kidney quinolinate phosphoribosyltransferase; no inhibition by nicotinamide mononucleotide, deamino-NAD+
-
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Bone Marrow Failure Disorders
The complex genetic landscape of familial MDS and AML reveals pathogenic germline variants.
Carcinoma
Dependence of tumor cell lines and patient-derived tumors on the NAD salvage pathway renders them sensitive to NAMPT inhibition with GNE-618.
Carcinoma, Non-Small-Cell Lung
Dependence of tumor cell lines and patient-derived tumors on the NAD salvage pathway renders them sensitive to NAMPT inhibition with GNE-618.
Glioblastoma
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Glioblastoma
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Glioma
NAD+ depletion radiosensitizes 2-DG-treated glioma cells by abolishing metabolic adaptation.
Glucose Intolerance
Tissue-specific regulation of sirtuin and nicotinamide adenine dinucleotide biosynthetic pathways identified in C57Bl/6 mice in response to high-fat feeding.
Lymphoma
Expression patterns of nicotinamide phosphoribosyltransferase and nicotinic acid phosphoribosyltransferase in human malignant lymphomas.
Neoplasms
A preclinical study on the rescue of normal tissue by nicotinic acid in high-dose treatment with APO866, a specific nicotinamide phosphoribosyltransferase inhibitor.
Neoplasms
Anti-tumor NAMPT inhibitor, KPT-9274, mediates gender-dependent murine anemia and nephrotoxicity by regulating SIRT3-mediated SOD deacetylation.
Neoplasms
Dependence of tumor cell lines and patient-derived tumors on the NAD salvage pathway renders them sensitive to NAMPT inhibition with GNE-618.
Neoplasms
Dual and Specific Inhibition of NAMPT and PAK4 By KPT-9274 Decreases Kidney Cancer Growth.
Neoplasms
Extensive regulation of nicotinate phosphoribosyltransferase (NAPRT) expression in human tissues and tumors.
Neoplasms
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Neoplasms
NAMPT and NAPRT1: novel polymorphisms and distribution of variants between normal tissues and tumor samples.
Neoplasms
Nicotinic Acid Phosphoribosyltransferase Regulates Cancer Cell Metabolism, Susceptibility to NAMPT Inhibitors, and DNA Repair.
Neoplasms
Supplementation of nicotinic acid with NAMPT inhibitors results in loss of in vivo efficacy in NAPRT1-deficient tumor models.
Neoplasms
Targeting the NAD Salvage Synthesis Pathway as a Novel Therapeutic Strategy for Osteosarcomas with Low NAPRT Expression.
Neoplasms
The complex genetic landscape of familial MDS and AML reveals pathogenic germline variants.
Neoplasms
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Neuroblastoma
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Neuroblastoma
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
nicotinate phosphoribosyltransferase deficiency
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Ovarian Neoplasms
Nicotinic Acid Phosphoribosyltransferase Regulates Cancer Cell Metabolism, Susceptibility to NAMPT Inhibitors, and DNA Repair.
Sarcoma
Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) activity by small molecule GMX1778 regulates reactive oxygen species (ROS)-mediated cytotoxicity in a p53- and nicotinic acid phosphoribosyltransferase1 (NAPRT1)-dependent manner.
Sarcoma
The small molecule GMX1778 is a potent inhibitor of NAD+ biosynthesis: strategy for enhanced therapy in nicotinic acid phosphoribosyltransferase 1-deficient tumors.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00022 - 48
5-phospho-alpha-D-ribose 1-diphosphate
1.16
ITP
-
good alternative substrate for ATP
0.0127 - 0.0155
nicotinic acid
0.00022
5-phospho-alpha-D-ribose 1-diphosphate
+/-0.07 microM, in presence of 1 mM NAD+, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification
0.00025
5-phospho-alpha-D-ribose 1-diphosphate
+/-0.06 microM, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification
0.013
5-phospho-alpha-D-ribose 1-diphosphate
-
reduced to 0.002 mM in the presence of ATP
0.013
5-phospho-alpha-D-ribose 1-diphosphate
-
reduced to 0.002 mM in the presence of ATP
0.02
5-phospho-alpha-D-ribose 1-diphosphate
-
in the coupled reaction, 200fold lower than in the uncoupled reaction
0.022
5-phospho-alpha-D-ribose 1-diphosphate
-
pncB gene, in the coupled reaction
0.0221
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.5, 37°C
0.024
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.03
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.0382
5-phospho-alpha-D-ribose 1-diphosphate
in the presence of phosphate, pH 7.5, 37°C
0.05
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.06 - 0.1
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.06 - 0.1
5-phospho-alpha-D-ribose 1-diphosphate
-
-
0.1
5-phospho-alpha-D-ribose 1-diphosphate
-
0.005 mM in the presence of ATP
2.3 - 48
5-phospho-alpha-D-ribose 1-diphosphate
-
mutant enzymes
4.5
5-phospho-alpha-D-ribose 1-diphosphate
-
-
4.5
5-phospho-alpha-D-ribose 1-diphosphate
-
pncB gene, in the uncoupled reaction
0.07
ATP
-
-
0.0005
nicotinate
-
in the presence of ATP
0.0008 - 0.0015
nicotinate
-
-
0.0015
nicotinate
-
pncB gene, in the coupled reaction
0.024
nicotinate
-
reduced to 0.0005 mM in the presence of ATP
0.0273
nicotinate
in the presence of phosphate, pH 7.5, 37°C
0.0443
nicotinate
pH 7.5, 37°C
0.22
nicotinate
-
reduced to 0.053 mM in the presence of ATP
0.22
nicotinate
-
reduced to 0.053 mM in the presence of ATP
0.29
nicotinate
-
pncB gene, in the uncoupled reaction
0.33 - 0.4
nicotinate
-
mutant enzymes
0.0127
nicotinic acid
+/-3.6 microM, in presence of 1 mM NAD+, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification
0.0155
nicotinic acid
+/-3.4 microM, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification
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0.0169
+/-0.006 micromol/min/mg, with respect to nicotinic acid, in presence of 1 mM NAD+, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification
0.0186
+/-0.006 micromol/min/mg, with respect to nicotinic acid, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification
0.0226
+/-0.0016 micromol/min/mg, with respect to 5-phospho-alpha-D-ribose 1-diphosphate, in presence of 1 mM NAD+, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification
0.0236
+/-0.0015 micromol/min/mg, with respect to 5-phospho-alpha-D-ribose 1-diphosphate, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification
0.08
-
recombinant enzyme from Escherichia coli strain BA203, pH and temperature not specified in the publication
0.74
-
recombinant enzyme from Escherichia coli strain BA204, pH and temperature not specified in the publication
12.24
-
recombinant enzyme from Escherichia coli strain BA207, pH and temperature not specified in the publication
160.5
-
DEAE-Sephadex fraction
17.31
-
recombinant enzyme from Escherichia coli strain BA206, pH and temperature not specified in the publication
18.74
recombinant enzyme from Escherichia coli strain BA016, pH and temperature not specified in the publication
23.72
-
recombinant enzyme from Escherichia coli strain BA207, pH and temperature not specified in the publication
421.2
-
Sephacryl fraction
457.3
-
phenyl-Sepharose fraction
0.7
-
recombinant enzyme from Escherichia coli strain BA002, pH and temperature not specified in the publication
0.7
recombinant enzyme from Escherichia coli strain BA002, pH and temperature not specified in the publication
1.01
recombinant enzyme from Escherichia coli strain BA015, pH and temperature not specified in the publication
1.01
-
recombinant enzyme from Escherichia coli strain BA206, pH and temperature not specified in the publication
10.54
-
recombinant enzyme from Escherichia coli strain BA014, pH and temperature not specified in the publication
10.54
recombinant enzyme from Escherichia coli strain BA014, pH and temperature not specified in the publication
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