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ATP + cyanophycin granule polypeptide-L-Asp + L-arginine
?
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-
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-
?
ATP + [L-Asp(4-L-Arg)]3-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]4 + L-Asp
-
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
ATP + [L-Asp(4-L-Arg)]n + L-Asp
[L-Asp(4-L-Arg)]n-L-Asp + ADP + phosphate
-
-
-
?
L-aspartic acid + ATP
poly-L-aspartic acid + ADP + phosphate
-
-
-
-
?
[L-Asp(4-L-Arg)]n + L-Asp + ATP
[L-Asp(4-L-Arg)]n-Asp + ADP + phosphate
-
a small amount of cyanophycin is required as a primer
no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30
-
?
additional information
?
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ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
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-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
study of expression profiles of the genes cphA1 and cphA2 and their dependence on the type of nitrogen supply in the medium
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
normal assay conditions
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
[L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase
no formation of AMP
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
study determined the effect of different light and nutrition conditions on cyanophycin granule formation
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-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
Thermosynechococcus vestitus
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-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
Thermosynechococcus vestitus
-
primer and product analysis using recombinantly expressed Tlr2170 protein, overview
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
[L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase
no formation of AMP
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
[L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase
no formation of AMP
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
?
additional information
?
-
-
without L-arginine 1.1% activity compared to the reaction mixture containing both substrates, no activity using L-lysine instead of L-arginine, no activity without addition of small amounts of cyanophycin as a primer for synthesis
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?
additional information
?
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cyanophycin accumulation is studied, comparison of nitrogen-fixing and non-nitrogen-fixing cyanobacteria
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?
additional information
?
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no activity without L-arginine, with L-lysine instead of L-arginine there is 15% activity compared to the normal conditions
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?
additional information
?
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the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29
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?
additional information
?
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the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29
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-
?
additional information
?
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negligible activities when L-arginine is omitted, no activity when L-arginine is replaced by L-glutamic acid, citrulline, ornithine, arginine amide, agmatine, or norvaline
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-
?
additional information
?
-
Thermosynechococcus vestitus
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CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview
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?
additional information
?
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Thermosynechococcus vestitus
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the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29. Recombinant Tlr2170 protein catalyzes in vitro cyanophycin synthesis in the absence of a primer. The Tlr2170 protein shows strict substrate specificity toward L-Asp and L-Arg
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-
?
additional information
?
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no product is formed if the peptide primer is blocked at the C-terminus or if L-arginine is solely supplied as substrate
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?
additional information
?
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cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers
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?
additional information
?
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the recombinant CphA49 exhibits strict primer dependency and broad substrate specificities. L-Lys and L-Glu can substitute for L-Asp, but with very low catalytic activity. No activity with L-Arg and L-citrulline, L-Arg and L-ornithine, L-Asp and L-citrulline, and L-Asp and L-ornithine
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-
?
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ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
[L-Asp(4-L-Arg)]n + L-Asp + ATP
[L-Asp(4-L-Arg)]n-Asp + ADP + phosphate
-
a small amount of cyanophycin is required as a primer
no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30
-
?
additional information
?
-
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
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-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
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-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
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study of expression profiles of the genes cphA1 and cphA2 and their dependence on the type of nitrogen supply in the medium
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-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
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?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
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-
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?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
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-
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-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
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-
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?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
Thermosynechococcus vestitus
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-
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-
?
ATP + [L-Asp(4-L-Arg)]n + L-Asp
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
-
-
?
additional information
?
-
-
without L-arginine 1.1% activity compared to the reaction mixture containing both substrates, no activity using L-lysine instead of L-arginine, no activity without addition of small amounts of cyanophycin as a primer for synthesis
-
-
?
additional information
?
-
Thermosynechococcus vestitus
-
CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview
-
-
?
additional information
?
-
cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers
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-
?
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engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli
expression in Escherichai coli
expression in Escherichia coli
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expression in Escherichia coli BL21(DE3)
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expression in Escherichia coli DH1 cells
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expression in Escherichia coli DH5alpha
expression in Escherichia coli TOP10 cells
expression in Pseudomonas putida ATCC 4359
expression in the wild-type Sinorhizobium meliloti 1021 and in a phbC-negative mutant
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functional expression in Nicotiana tabacum var. Petit Havana SRI targeted to the chloroplasts using the CaMV 35S promoter and a translocation pathway signal sequence, the phenotypic abnormalities are reduced by this way, cyanophycin accumulation in chloroplasts, overview
Thermosynechococcus vestitus
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gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3)
Thermosynechococcus vestitus
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gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3)
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gene cphA49 cloned from deep-sea sediment metagenomic library with degenerated primers, amplified from the fss49 fosmid by PCR, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of His-tagged cphA49 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue
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gene cphA6803, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method
gene cphA7120, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method
expression in Escherichia coli TOP10 cells
expression in Escherichia coli TOP10 cells
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synthesis
-
expression of cyanophycin synthase in wild-type Sinorhizobium meliloti 1021 and in a phbC-negative mutant. Yeast mannitol broth yields the highest cyanophycin contents in both Sinorhizobium meliloti 1021 strains. Supplying the medium with isopropyl-beta-D-thiogalactopyranoside, aspartic acid, and arginine enhances cyanophycin contents about 2.5- and 2.8fold. Varying the nitrogen-to-carbon ratio in the medium enhanced the cyanophycin content further to 43.8% w/w of cell dry weight. Cyanophycin from the Sinorhizobium meliloti strains consists of equimolar amounts of aspartic acid and arginine and contains no other amino acids even if the medium is supplemented with glutamic acid, citrulline, ornithine, or lysine. Cyanophycin isolated from Sinorhizobium meliloti exhibits average molecular weights between 20 and 25 kDa. Cyanophycin isolated after expression in Escherichia coli S17-1 exhibits average molecular weight between 22 and 30 kDa. Co-expression of cyanophycinase from Anabaena sp. PCC7120 encoded by cphB17120 in cphA17120-positive Escherichia coli S17-1, Sinorhizobium meliloti 1021, and its phbC-negative mutant gives cyanophycinase activities in crude extracts, and no CGP granules occur
synthesis
expression of the cyanophycin synthetase of Synechocystis sp. PCC 6308 in Pseudomonas putida ATCC 4359 using an optimised medium for cultivation, results in synthesis of insoluble cyanophycin up to 14.7 w/w and soluble cyanophycin amounting up to 28.7 w/w of the cell dry matter. The soluble CGP is composed of 50.4 mol% aspartic acid, 32.7 mol% arginine, 8.7 mol% citrulline and 8.3 mol% lysine. The insoluble cyanophycin contains less than 1 mol% of citrulline. Using a mineral salt medium with 1.25 or 2% w/v sodium succinate, respectively, plus 23.7 mM L-arginine, the cells synthesise insoluble cyanophycin amounting up to 25% to 29% of the CDM with only a very low citrulline content
synthesis
Thermosynechococcus vestitus
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restriction of cyanophycin accumulation to the potato tubers by using the cyanophycin synthetase gene from Thermosynechococcus elongatus BP-1, under the control of the tuber-specific class 1 promoter. Tuber-specific cytosolic expression by pB33-cphATe as well as tuber-specific plastidic expression by pB33-PsbYcphATe results in significant polymer accumulation solely in the tubers. In plants transformed with pB33-cphATe, both cyanophycin synthetase and cyanophycin are detected in the cytoplasm leading to an increase up to 2.3% cyanophycin of dry weight and resulting in small and deformed tubers. In B33-PsbY-cphATe tubers, cyanophycin synthetase and cyanophycin are exclusively found in amyloplasts leading to a cyanophycin accumulation up to 7.5% of dry weight. These tubers are normal in size, some clones show reduced tuber yield and sometimes exhibit brown sunken staining starting at tubers navel. During a storage period over of 32 weeks of one selected clone, the cyanophycin content was stable in B33-PsbYcphATe tubers but the stress symptoms increased. Nitrogen fertilization in the greenhouse does not lead not to an increased cyanophycin yield, slightly reduced protein content, decreased starch content, and changes in the amounts of bound and free arginine and aspartate
synthesis
synthesis of cyanophycin using an anabolism-based media-dependent plasmid addiction system to enhance plasmid stability, and a process based on a modified mineral salts medium yielding a cyanophycin content of 42% w/w at the maximum without the addition of amino acids to the medium. This plasmid addiction system is based on different lysine biosynthesis pathways and consists of a knockout of the chromosomal dapE that disrupts the native succinylase pathway in Escherichia coli and the complementation by the plasmid-encoded artificial aminotransferase pathway mediated by the dapL gene from Synechocystis sp. PCC 6308, which allows the synthesis of the essential lysine precursor L,L-2,6-diaminopimelate. This plasmid also harbors an engineered cyanophycin synthetase gene responsible for cyanophycin production. Cultivation experiments reveal an at least 4.5fold enhanced production of cyanophycin in comparison to control cultivations
synthesis
-
cyanophycin produced in Escherichia coli is composed of 50% of aspartic acid, 45% of arginine, and 3.5% of lysine, and exhibits a homogenous molecular mass of 35 kDa. Cultivation in presence of arginine, aspartic acid, lysine and glucose with the minimal resource leads to 1.72 g/l soluble cyanophycin
synthesis
-
expression of cyanophycin synthase in wild-type Sinorhizobium meliloti 1021 and in a phbC-negative mutant. Yeast mannitol broth yields the highest cyanophycin contents in both Sinorhizobium meliloti 1021 strains. Supplying the medium with isopropyl-beta-D-thiogalactopyranoside, aspartic acid, and arginine enhances cyanophycin contents about 2.5- and 2.8fold. Varying the nitrogen-to-carbon ratio in the medium enhanced the cyanophycin content further to 43.8% w/w of cell dry weight. Cyanophycin from the Sinorhizobium meliloti strains consists of equimolar amounts of aspartic acid and arginine and contains no other amino acids even if the medium is supplemented with glutamic acid, citrulline, ornithine, or lysine. Cyanophycin isolated from Sinorhizobium meliloti exhibits average molecular weights between 20 and 25 kDa. Cyanophycin isolated after expression in Escherichia coli S17-1 exhibits average molecular weight between 22 and 30 kDa. Co-expression of cyanophycinase from Anabaena sp. PCC7120 encoded by cphB17120 in cphA17120-positive Escherichia coli S17-1, Sinorhizobium meliloti 1021, and its phbC-negative mutant gives cyanophycinase activities in crude extracts, and no CGP granules occur
-
synthesis
-
synthesis of cyanophycin using an anabolism-based media-dependent plasmid addiction system to enhance plasmid stability, and a process based on a modified mineral salts medium yielding a cyanophycin content of 42% w/w at the maximum without the addition of amino acids to the medium. This plasmid addiction system is based on different lysine biosynthesis pathways and consists of a knockout of the chromosomal dapE that disrupts the native succinylase pathway in Escherichia coli and the complementation by the plasmid-encoded artificial aminotransferase pathway mediated by the dapL gene from Synechocystis sp. PCC 6308, which allows the synthesis of the essential lysine precursor L,L-2,6-diaminopimelate. This plasmid also harbors an engineered cyanophycin synthetase gene responsible for cyanophycin production. Cultivation experiments reveal an at least 4.5fold enhanced production of cyanophycin in comparison to control cultivations
-
synthesis
-
expression of the cyanophycin synthetase of Synechocystis sp. PCC 6308 in Pseudomonas putida ATCC 4359 using an optimised medium for cultivation, results in synthesis of insoluble cyanophycin up to 14.7 w/w and soluble cyanophycin amounting up to 28.7 w/w of the cell dry matter. The soluble CGP is composed of 50.4 mol% aspartic acid, 32.7 mol% arginine, 8.7 mol% citrulline and 8.3 mol% lysine. The insoluble cyanophycin contains less than 1 mol% of citrulline. Using a mineral salt medium with 1.25 or 2% w/v sodium succinate, respectively, plus 23.7 mM L-arginine, the cells synthesise insoluble cyanophycin amounting up to 25% to 29% of the CDM with only a very low citrulline content
-
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Hai, T.; Oppermann-Sanio, F.B.; Steinbchel, A.
Molecular characterization of a thermostable cyanophycin synthetase from the thermophilic cyanobacterium Synechococcus sp. strain MA19 and in vitro synthesis of cyanophycin and related polyamides
Appl. Environ. Microbiol.
68
93-101
2002
Synechococcus sp. MA19 (Q8VTA5)
brenda
Aboulmagd, E.; Oppermann-Sanio, F.B.; Steinbchel, A.
Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308
Arch. Microbiol.
174
297-306
2000
Synechocystis sp., Synechocystis sp. PCC 6804
brenda
Li H.; Sherman, D.M.; Bao, S.; Sherman, L.A.
Pattern of cyanophycin accumulation in nitrogen-fixing and non-nitrogen-fixing cyanobacteria
Arch. Microbiol.
176
9-18
2001
Crocosphaera subtropica ATCC 51142 (Q9KGY4)
brenda
Ziegler, K.; Diener, A.; Herpin, C.; Richter, R.; Deutzmann, R.; Lockau, W.
Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-poly-L-aspartate (cyanophycin)
Eur. J. Biochem.
254
154-159
1998
Trichormus variabilis (O86109)
brenda
Berg, H.; Ziegler, K.; Piotukh, K.; Baier, K.; Lockau, W.; Volkmer-Engert, R.
Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin)
Eur. J. Biochem.
267
5561-5570
2000
Trichormus variabilis ATCC 29413
brenda
Allen, M.M.; Hutchison, F.; Weathers, P.J.
Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308
J. Bacteriol.
141
687-693
1980
Synechocystis sp. PCC 6803
brenda
Picossi, S.; Valadares, A.; Flores, E.; Herrero, A.
Nitrogen-regulated genes for the metabolism of cyanophycin, a bacterial nitrogen reserve polymer
J. Biol. Chem.
279
11582-11592
2004
Anabaena sp.
brenda
Krehenbrink, M.; Steinbchel, A.
Partial purification and characterization of a non-cyanobacterial cyanophycin synthetase from Acinetobacter calcoaceticus strain ADP1 with regard to substrate specificity, substrate affinity and binding to cyanophycin
Microbiology
150
2599-2608
2004
Acinetobacter calcoaceticus
brenda
Elbahloul, Y.; Steinbuechel, A.
Engineering the genotype of Acinetobacter sp. strain ADP1 to enhance biosynthesis of cyanophycin
Appl. Environ. Microbiol.
72
1410-1419
2006
Acinetobacter sp. (Q6FCQ7), Acinetobacter sp. ADP1 (Q6FCQ7)
brenda
Hai, T.; Frey, K.M.; Steinbuechel, A.
Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli
Appl. Environ. Microbiol.
72
7652-7660
2006
Nostoc ellipsosporum (Q0H8A5)
brenda
Diniz, S.C.; Voss, I.; Steinbuechel, A.
Optimization of cyanophycin production in recombinant strains of Pseudomonas putida and Ralstonia eutropha employing elementary mode analysis and statistical experimental design
Biotechnol. Bioeng.
93
698-717
2006
Anabaena sp., Synechocystis sp., Anabaena sp. PCC 7120, Synechocystis sp. PCC6308
brenda
Kolodny, N.H.; Bauer, D.; Bryce, K.; Klucevsek, K.; Lane, A.; Medeiros, L.; Mercer, W.; Moin, S.; Park, D.; Petersen, J.; Wright, J.; Yuen, C.; Wolfson, A.J.; Allen, M.M.
Effect of nitrogen source on cyanophycin synthesis in Synechocystis sp. strain PCC 6308
J. Bacteriol.
188
934-940
2006
Synechocystis sp.
brenda
Steinle, A.; Oppermann-Sanio, F.B.; Reichelt, R.; Steinbuechel, A.
Synthesis and accumulation of cyanophycin in transgenic strains of Saccharomyces cerevisiae
Appl. Environ. Microbiol.
74
3410-3418
2008
Synechocystis sp.
brenda
Arai, T.; Kino, K.
A cyanophycin synthetase from Thermosynechococcus elongatus BP-1 catalyzes primer-independent cyanophycin synthesis
Appl. Microbiol. Biotechnol.
81
69-78
2008
Thermosynechococcus vestitus
brenda
Hai, T.; Lee, J.S.; Kim, T.J.; Suh, J.W.
The role of the C-terminal region of cyanophycin synthetase from Nostoc ellipsosporum NE1 in its enzymatic activity and thermostability: A key function of Glu(856)
Biochim. Biophys. Acta
1794
42-49
2008
Nostoc ellipsosporum, Nostoc ellipsosporum NE1
brenda
Huehns, M.; Neumann, K.; Hausmann, T.; Ziegler, K.; Klemke, F.; Kahmann,.; Staiger, D.; Lockau, W.; Pistorius, E.K.; Broer, I.
Plastid targeting strategies for cyanophycin synthetase to achieve high-level polymer accumulation in Nicotiana tabacum
Plant Biotechnol. J.
6
321-336
2008
Thermosynechococcus vestitus
brenda
Huehns, M.; Neumann, K.; Hausmann, T.; Klemke, F.; Lockau, W.; Kahmann, U.; Kopertekh, L.; Staiger, D.; Pistorius, E.K.; Reuther, J.; Waldvogel, E.; Wohlleben, W.; Effmert, M.; Junghans, H.; Neubauer, K.; Kragl, U.; Schmidt, K.; Schmidtke, J.; Broer, I.
Tuber-specific cphA expression to enhance cyanophycin production in potatoes
Plant Biotechnol. J.
7
883-898
2009
Thermosynechococcus vestitus
brenda
Abd-El-Karem, Y.; Elbers, T.; Reichelt, R.; Steinbuechel, A.
Heterologous expression of Anabaena sp. PCC7120 cyanophycin metabolism genes cphA1 and cphB1 in Sinorhizobium (Ensifer) meliloti 1021
Appl. Microbiol. Biotechnol.
89
1177-1192
2011
Anabaena sp., Anabaena sp. PCC 7120
brenda
Kroll, J.; Klinter, S.; Steinbuechel, A.
A novel plasmid addiction system for large-scale production of cyanophycin in Escherichia coli using mineral salts medium
Appl. Microbiol. Biotechnol.
89
593-604
2011
Synechocystis sp. (P56947), Synechocystis sp. PCC 6308 (P56947)
brenda
Wiefel, L.; Broeker, A.; Steinbuechel, A.
Synthesis of a citrulline-rich cyanophycin by use of Pseudomonas putida ATCC 4359
Appl. Microbiol. Biotechnol.
90
1755-1762
2011
Synechocystis sp. (P56947), Synechocystis sp. PCC 6308 (P56947)
brenda
Meussen, B.J.; Weusthuis, R.A.; Sanders, J.P.; Graaff, L.H.
Production of cyanophycin in Rhizopus oryzae through the expression of a cyanophycin synthetase encoding gene
Appl. Microbiol. Biotechnol.
93
1167-1174
2012
Anabaena sp. (P58572), Synechocystis sp. (P73833), Anabaena sp. PCC 7120 (P58572)
brenda
Du, J.; Li, L.; Ding, X.; Hu, H.; Lu, Y.; Zhou, S.
Isolation and characterization of a novel cyanophycin synthetase from a deep-sea sediment metagenomic library
Appl. Microbiol. Biotechnol.
97
8619-8628
2013
uncultured bacterium (M9UYB0)
brenda
Burnat, M.; Herrero, A.; Flores, E.
Compartmentalized cyanophycin metabolism in the diazotrophic filaments of a heterocyst-forming cyanobacterium
Proc. Natl. Acad. Sci. USA
111
3823-3828
2014
Anabaena sp. (P58572), Anabaena sp. PCC 7120 (P58572)
brenda
Liu, H.; Ray, W.K.; Helm, R.F.; Popham, D.L.; Melville, S.B.
Analysis of the spore membrane proteome in Clostridium perfringens implicates cyanophycin in spore assembly
J. Bacteriol.
198
1773-1782
2016
Clostridium perfringens (Q0SQX0), Clostridium perfringens SM101 (Q0SQX0)
brenda
Du, J.; Li, L.; Zhou, S.
Enhanced cyanophycin production by Escherichia coli overexpressing the heterologous cphA gene from a deep sea metagenomic library
J. Biosci. Bioeng.
123
239-244
2017
unidentified microorganism
brenda
Nausch, H.; Hausmann, T.; Ponndorf, D.; Huehns, M.; Hoedtke, S.; Wolf, P.; Zeyner, A.; Broer, I.
Tobacco as platform for a commercial production of cyanophycin
New Biotechnol.
33
842-851
2016
Thermosynechococcus vestitus
brenda