Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ADP + methotrexate-Glu3 + phosphate
ATP + methotrexate-Glu2 + L-glutamate
-
-
-
-
?
ADP + methotrexate-Glu4 + phosphate
ATP + methotrexate-Glu3 + L-glutamate
-
-
-
-
?
ADP + phosphate + 7,8-dihydropteroyl-Glu
ATP + 7,8-dihydropteroate + L-glutamate
ATP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butyric acid + L-glutamate
ADP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butanoyl-Glu + phosphate
-
-
-
?
ATP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butyric acid + L-glutamate
ADP + (2S)-2-(o-fluoro-p-(N-(2,7-dimethyl-4-oxo-3,4-dihydroquinazolin-6-yl)methyl)-N-(prop-2-ynyl-amino)benzamido)-4-(tetrazol-5-yl)butanoyl-L-Glu + phosphate
-
-
-
?
ATP + (6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6R)-10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + (6R)-5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + (6R)-5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
ATP in form of MgATP2-
-
-
?
ATP + (6R,S)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-gamma-Glu
-
-
-
-
ir
ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
ATP + (6RS)-5-methyl-H4-homofolate + Glu
ADP + phosphate + homofolyl-Glun
-
-
-
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
ATP + (6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
poor substrate
-
?
ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
poor substrate
-
?
ATP + (6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5-methyl-5,6,7,8-tetrahydropteroyldiglutamate
-
-
-
-
?
ATP + (S)-2(5-(((1,2-dihydro-3-methyl-1-oxobenzo-(f)quinazolin-9-yl)methyl)-1-oxo-2-isoindolinyl))glutaric acid + L-glutamate
ADP + (S)-2(5-(((1,2-dihydro-3-methyl-1-oxobenzo-(f)quinazolin-9-yl)methyl)-1-oxo-2-isoindolinyl))glutaryl-L-Glu + phosphate
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 3,3-difluoromethotrexate + L-glutamate
?
ATP + 3,5-difluoromethotrexate + Glu
ADP + phosphate + 3,5-difluoromethotrexyl-Glu
ATP + 3,5-difluoropteroyl-Glu + Glu
ADP + phosphate + 3,5-difluoropteroyl-Glu2
ATP + 3-(N-phosphonoacetyl)amino-2-L-(N-pteroylamino)propanoic acid + L-glutamate
ADP + 3-(N-phosphonoacetyl)amino-2-L-(N-pteroylamino)propanoyl-Glu + phosphate
-
much less effective than folic acid
-
?
ATP + 3-N-(methoxyphosphono)acetylamino-2-L-(N-pteroylamino)propanoic acid + L-glutamate
ADP + 3-N-(methoxyphosphono)acetylamino-2-L-(N-pteroylamino)propanoyl-Glu + phosphate
-
much less effective than folic acid
-
?
ATP + 4-amino-4-deoxypteroyl-L-ornithine + L-glutamate
?
-
-
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate
ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-L-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)3
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
ATP + 5,10-methylene-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu3
ATP + 5,6,7,8-tetrahydrofolic acid + L-Glu
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
ATP + 5,6,7,8-tetrahydrofolyl-beta-L-Ala-L-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-beta-L-Ala-L-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolyl-gamma-(L-Glu)2 + L-Glu
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-gamma-(L-Glu)3
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroate + Glu
ADP + phosphate + 5,6,7,8-terahydropteroyl-Glu
ATP + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glun
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu + L-Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu4
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu5
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu4 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu5
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu6
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
ATP + 5,6,7,8-tetrahydropteroyl-Gly-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Gly-Glu2
ATP + 5-formyl-5,6,7,8-tetrahydrofolate + L-glutamate
ADP + 5-formyl-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
ATP + 5-methyl-H4-homofolate + L-glutamate
ADP + phosphate + 5-methyl-H4-homofolyl-Glu
-
-
-
-
?
ATP + 6-R 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
at similar rate as that seen with aminopterin as substrate, endogenous enzyme isolated from liver. More efficient than aminopterin, recombinant liver enzyme
-
?
ATP + 6-S 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu3
-
more efficient than aminopterin, recombinant liver enzyme
-
?
ATP + 7,8-dihydropteroate + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glu
ATP + 7,8-dihydropteroate + L-Glu
ADP + phosphate + 7,8-dihydropteroyl-L-Glu
-
-
-
?
ATP + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu + L-glutamate
ADP + phosphate + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-(5-aminovaleryl)-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-(5-aminovaleryl)-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-4-aminobutanoyl-Glun
-
-
n: 3-5
?
ATP + 7,8-dihydropteroyl-beta-Ala-Glu + Glu
ADP + phosphate + 7,8-dihydropteroyl-beta-Ala-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glu + L-glutamate
ADP + phosphate + 7,8-dihydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glu + L-glutamate
ADP + phosphate + 7,8-dihydropteroyl-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
ATP + 7-hydroxymethotrexate + Glu
ADP + phosphate + 7-hydroxymethotrexyl-Glu
-
-
-
-
?
ATP + 9-methylpteroyl-L-glutamic acid + Glu
ADP + phosphate + 9-methylpteroyldiglutamate
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
ATP + aminopterin + L-glutamate
?
ATP + dichloromethotrexate + Glu
ADP + phosphate + dichloromethotrexyl-Glu
-
-
-
-
?
ATP + dihydroaminopterin + Glu
ADP + phosphate + dihydroaminopteryl-Glu
ATP + dihydropteroic acid + L-glutamate
ADP + dihydropteroyl-Glu
-
enhancement of ATP binding is observed in the presence of this substrate. The substrate causes a conformational change in the inactive fraction of the enzyme which allows it to bind ATP
-
?
ATP + homofolate + Glu
ADP + phosphate + L-homocysteyl-Glu
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydrofolate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydrofolate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
ADP + phosphate + (6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
-
-
?
ATP + L-glutamate + tetrahydrofolate-Glu
ADP + phosphate + tetrahydrofolyl-Glu2
-
assay at pH 10, 37°C
-
-
?
ATP + L-homocysteate + Glu
ADP + phosphate + (6RS)-5-methyl-H4-homofolyl-Glu
-
-
-
-
?
ATP + methotrexate + 3,3-difluoroglutamate
ADP + phosphate + methotrexyl-3,3-difluoroglutamate
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
ATP + methotrexate + L-glutamate
ADP + phosphate + L-glutamyl-methotrexate
-
-
-
-
?
ATP + methotrexate + L-glutamate
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
ATP + methotrexate-phosphinate + L-glutamate
?
-
-
-
?
ATP + methotrexate-phosphonate + L-glutamate
?
-
-
-
?
ATP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-glutamic acid + L-glutamate
ADP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-Glu2 + phosphate
ATP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid + L-glutamate
ADP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-Glu2 + phosphate
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)Glu2
-
folic acid, poor substrate
-
?
ATP + Nalpha-(4-amino-4-deoxy-5,8-diazapteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(4-amino-4-deoxy-5,chloropteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(4-amino-4-deoxy-8-deazapteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(4-amino-4-deoxy-N10-methylpteroyl)-Nepsilon-(phosphonoacetyl)-L-lysine + L-glutamate
?
-
much less effective than folic acid
-
?
ATP + Nalpha-(4-amino-4-deoxypteroyl)-Ndelta-hemiphthaloyl-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(5,8-dideaza-5-chloropteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-(5,8-dideazapteroyl)-L-ornithine + L-glutamate
?
-
-
-
?
ATP + Nalpha-pteroyl-L-ornithine + L-glutamate
?
-
-
-
?
ATP + pemetrexed + Glu
ADP + phosphate + pemetrexyl-Glu
-
-
-
-
?
ATP + pteroyl-(4-aminobutanoyl)-Glu + Glu
ADP + phosphate + pteroyl-(4-aminobutanoyl)-Glu2
-
-
-
-
?
ATP + pteroyl-(5-aminopentanoyl)-Glu + Glu
ADP + phosphate + pteroyl-(5-aminopentanoyl)-Glu2
-
-
-
-
?
ATP + pteroyl-beta-Ala-Glu + Glu
ADP + phosphate + pteroyl-beta-Ala-Glu2
-
-
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
ATP + pteroyl-Glu3 + L-glutamate
ADP + phosphate + pteroyl-Glu4
ATP + pteroyl-Glu4 + L-glutamate
ADP + phosphate + pteroyl-Glu5
-
-
-
-
?
ATP + pteroyl-Glu5 + L-glutamate
ADP + phosphate + pteroyl-Glu6
-
-
-
-
?
ATP + tetrahydroaminopterin + Glu
ADP + phosphate + tetrahydroaminopteryl-Glu
-
-
-
?
ATP + tetrahydrofolate-Glu + L-glutamate
ADP + phosphate + tetrahydrofolyl-Glu2
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
dATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
dATP + aminopterin + Glu
dADP + phosphate + aminopteryl-Glu
-
112% of the activity relative to ATP
-
-
?
dGTP + aminopterin + Glu
dGDP + phosphate + aminopteryl-Glu
-
37% of the activity relative to ATP
-
-
?
GTP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
GDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
ITP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu
IDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
ITP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
IDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
L-glutamate + ATP + aminopterin
?
-
assay at 37°C
-
-
?
UTP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
additional information
?
-
ADP + phosphate + 7,8-dihydropteroyl-Glu
ATP + 7,8-dihydropteroate + L-glutamate
-
-
-
-
?
ADP + phosphate + 7,8-dihydropteroyl-Glu
ATP + 7,8-dihydropteroate + L-glutamate
-
-
-
-
?
ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
-
not as effective as 5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
?
ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
-
ir
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 3,3-difluoromethotrexate + L-glutamate
?
-
-
-
-
?
ATP + 3,3-difluoromethotrexate + L-glutamate
?
-
-
-
-
?
ATP + 3,5-difluoromethotrexate + Glu
ADP + phosphate + 3,5-difluoromethotrexyl-Glu
-
-
-
?
ATP + 3,5-difluoromethotrexate + Glu
ADP + phosphate + 3,5-difluoromethotrexyl-Glu
-
-
-
?
ATP + 3,5-difluoropteroyl-Glu + Glu
ADP + phosphate + 3,5-difluoropteroyl-Glu2
-
-
-
?
ATP + 3,5-difluoropteroyl-Glu + Glu
ADP + phosphate + 3,5-difluoropteroyl-Glu2
-
-
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
lometrexol
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5,10-dideaza-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + 5,10-dideaza-5,6,7,8-tetrahydrofolyl-L-Glu + phosphate
-
lometrexol
-
?
ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate
ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5,10-dideazatetrahydrofolic acid + L-glutamate
ADP + 5,10-dideazatetrahydrofolyl-L-Glu + phosphate
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 5
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 7
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 6
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n:1
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolate-Glu2 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu3
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
?
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu
-
-
?
ATP + 5,6,7,8-tetrahydropteroate + Glu
ADP + phosphate + 5,6,7,8-terahydropteroyl-Glu
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroate + Glu
ADP + phosphate + 5,6,7,8-terahydropteroyl-Glu
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glun
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-4-aminobutanoyl-Glun
-
-
n: 3-5
?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
ATP in form of MgATP2-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
ATP in form of MgATP2-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
gaba i.e. gamma-aminobutanoyl
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-gamma-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
gaba i.e. gamma-aminobutanoyl
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 3
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 5
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 4
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 7
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 6
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Gly-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Gly-Glu2
-
-
-
-
?
ATP + 5,6,7,8-tetrahydropteroyl-Gly-Glu + Glu
ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Gly-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 2
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
n: 1
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
?
ATP + 5-methyl-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
ADP + phosphate + 5-methyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
ATP + 7,8-dihydropteroate + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glu
-
-
-
-
?
ATP + 7,8-dihydropteroate + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glu
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
-
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 5
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 5
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 2
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 2
-
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
?
ATP + 7,8-dihydropteroyl-Glun + Glu
ADP + phosphate + 7,8-dihydropteroyl-Glun+1
-
n: 1
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
most active substrate
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
-
-
?
ATP + aminopterin + Glu
ADP + phosphate + aminopteryl-Glu
-
-
products with a glutamate chain length of 2-4 are formed
?
ATP + aminopterin + L-glutamate
?
-
-
-
?
ATP + aminopterin + L-glutamate
?
-
-
-
?
ATP + aminopterin + L-glutamate
?
-
-
-
?
ATP + aminopterin + L-glutamate
?
-
more efficient than 6-S 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2 and 6-R 5,10-dideaza-5,6,7,8-tetrahydrofolate-Glu2, recombinant enzyme from leukemic cells
-
?
ATP + dihydroaminopterin + Glu
ADP + phosphate + dihydroaminopteryl-Glu
-
-
-
?
ATP + dihydroaminopterin + Glu
ADP + phosphate + dihydroaminopteryl-Glu
-
-
-
-
?
ATP + methotrexate + 3,3-difluoroglutamate
ADP + phosphate + methotrexyl-3,3-difluoroglutamate
-
-
-
?
ATP + methotrexate + 3,3-difluoroglutamate
ADP + phosphate + methotrexyl-3,3-difluoroglutamate
-
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
weak
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
?
ATP + methotrexate + Glu
ADP + phosphate + methotrexyl-Glu
-
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
CHO cells expressing the human enzyme accumulate more metabolized drug than CHO cells expressing equivalent levels of endogenous hamster enzyme. Cells expressing only the mitochondrial isozyme do not metabolize this substrate
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
T-ALL and AML patients show inefficient polyglutamylation due to lower enzyme activity
-
?
ATP + methotrexate-Glu + L-glutamate
ADP + methotrexate-Glu2 + phosphate
-
-
-
?
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
-
n: 3
-
?
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
-
n: 5
-
?
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
-
n:4
-
?
ATP + methotrexate-Glun + Glu
ADP + phosphate + methotrexyl-Glun+1
-
n: 2
-
?
ATP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-glutamic acid + L-glutamate
ADP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-Glu2 + phosphate
-
-
-
?
ATP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-glutamic acid + L-glutamate
ADP + N-(4-(2(2-amino-3,4-dihydro-4-oxo-7H-pyrolo-(2,3-d)pyrimidine-5-yl)ethyl)-benzoyl)-L-Glu2 + phosphate
-
pemetrexed
-
?
ATP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid + L-glutamate
ADP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-Glu2 + phosphate
-
-
-
?
ATP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-glutamic acid + L-glutamate
ADP + N-(5-(N-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-N-methylamino)-2-thenoyl)L-Glu2 + phosphate
-
raltitrexed
-
?
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
-
folic acid
-
?
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
-
folic acid
-
?
ATP + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid + L-glutamate
ADP + phosphate + N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)-Glu2
-
folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
-
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
-
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 2
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
-
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 3
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 5
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 2
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 4
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 7
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 6
-
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
?
ATP + pteroyl-Glu + Glu
ADP + phosphate + pteroylmonoglutamyl-Glu
-
n: 1, i.e. pteroylmonoglutamate, folic acid
-
-
?
ATP + pteroyl-Glu3 + L-glutamate
ADP + phosphate + pteroyl-Glu4
-
-
-
-
?
ATP + pteroyl-Glu3 + L-glutamate
ADP + phosphate + pteroyl-Glu4
-
-
-
-
?
ATP + tetrahydrofolate-Glu + L-glutamate
ADP + phosphate + tetrahydrofolyl-Glu2
-
-
-
?
ATP + tetrahydrofolate-Glu + L-glutamate
ADP + phosphate + tetrahydrofolyl-Glu2
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate
ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n+1
-
-
-
?
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
CTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
CDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
at 13% of the activity relative to ATP
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
as effective as ATP
-
-
?
dATP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
dADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
UTP + 5,6,7,8-tetrahydropteroyl-Glu + Glu
UDP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu2
-
-
-
-
?
additional information
?
-
-
uses 5,10-methylenen-5,6,7,8-tetrahydropteroylpolyglutamate as the preferred, and sometimes only polyglutamate substrate
-
-
?
additional information
?
-
-
enzyme in biosynthesis of folylpolyglutamates , the most universal functions of folylpolyglutamates: 1. Actual cofactors in vivo for folate dependent enzymes, 2. Inhibitor of folate dependent enzymes for which they are not substrates, 3. Increases retention of folates after they are transported into cells as monoglutamates
-
-
?
additional information
?
-
-
dATP and UTP can act as substrate
-
-
?
additional information
?
-
-
dATP and UTP can act as substrate
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
pteroate and pteroylmonoglutamate substrates are utilized much more effectively than the polyglutamate derivatives. The most effective substrates are: 5,6,7,8-tetrahydropteroate, tetrahydrofolate, 5,10-methylene-tetrahydrofolate. The most effective diglutamate substrate is : 5,10-methylene-5,6,7,8-tetrahydropteroyldiglutamate
-
-
?
additional information
?
-
-
monoglutamyl form and polyglutamyl forms of 5,6,7,8-tetrahydropteroate are the preferred substrates
-
-
?
additional information
?
-
-
enzyme of folate biosynthetic pathway
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
enzyme also posseses dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
the enzyme catalyzes the MgATP-dependent ligation of L-glutamate to tetrahydrofolate coenzymes or to antifolates at the gamma-carboxyl of the terminal glutamate to form polyglutamate derivatives
-
?
additional information
?
-
-
enzyme in biosynthesis of folylpolyglutamates , the most universal functions of folylpolyglutamates: 1. Actual cofactors in vivo for folate dependent enzymes, 2. Inhibitor of folate dependent enzymes for which they are not substrates, 3. Increases retention of folates after they are transported into cells as monoglutamates
-
-
?
additional information
?
-
-
mitochondrial enzyme is required for mitochondrial one carbon metabolism and for normal one carbon flux in the cytosol
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
depressed activity of the enzyme is the predominant mechanism of high level resistance to polyglutamylation-dependent antifolates
-
?
additional information
?
-
-
mitochondrial enzyme activity is required for mitochondrial folate accumulation, and cells lacking this isozyme are auxotrophic for glycine. Overexpression of cytosolic enzyme does not complement the lack of mitochondrial activity
-
?
additional information
?
-
-
enzyme catalyzes the addition of glutamate residues to folates and antifolates to form the physiological active coenzymatic forms of the vitamin and more potent anti-folate agents
-
-
?
additional information
?
-
-
central enzyme in establishing and maintaining folylpolyglutamate pools in whole cells
-
?
additional information
?
-
-
the enzyme catalyzes the formation of an amide bond between glutamic acid and the gamma-carboxyl group of any of the naturally occurring folate compounds
-
?
additional information
?
-
-
the enzyme catalyzes the MgATP-dependent addition of glutamate residues to the gamma-carboxyl of folates to generate folylpolyglutamates
-
?
additional information
?
-
-
the enzyme synthesizes folate and antifolate poly(gamma-glutamate) metabolites
-
?
additional information
?
-
-
folylpoly-gamma-glutamate synthetase can catalyze the processive addition of approximately four glutamate residues to (6R)-5,10-dideaza-5,6,7,8-tetrahydropteroylglutamic acid. The degree of processivity is dependent on the concentration of the folate substrate, thus suggesting a mechanism for the regulation of folate polyglutamate synthesis in cells
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
the enzyme catalyzes the MgATP-dependent ligation of L-glutamate to tetrahydrofolate coenzymes or to antifolates at the gamma-carboxyl of the terminal glutamate to form polyglutamate derivatives
-
?
additional information
?
-
-
the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell
-
?
additional information
?
-
there are two possible sites for binding folate substrates. The di- and triglutamate substrates can be accommodated in both sites in a way appropriate for the ligation reaction, but only the new pocket appears to bind mTHF and allow the initial ligation reaction. It is suggested that the new pocket plays an essential role in the first turnover to create folylpoly-glutamates
-
-
?
additional information
?
-
-
there are two possible sites for binding folate substrates. The di- and triglutamate substrates can be accommodated in both sites in a way appropriate for the ligation reaction, but only the new pocket appears to bind mTHF and allow the initial ligation reaction. It is suggested that the new pocket plays an essential role in the first turnover to create folylpoly-glutamates
-
-
?
additional information
?
-
-
most effective substrates are: 7,8-dihydrofolate, 5,6,7,8-tetrahydrofolate, 5,10-methylenetetrahydrofolate, 10-formyltetrahydrofolate, 5-formyltetrahydrofolate
-
-
?
additional information
?
-
-
alterations in the amino terminal structure of the enzyme may influence its catalytic and/or substrate binding properties
-
?
additional information
?
-
-
depressed activity of the enzyme is the predominant mechanism of high level resistance to polyglutamylation-dependent antifolates
-
?
additional information
?
-
-
the enzyme catalyzes the consecutive addition of one or more L-glutamyl moieties to the gamma-carboxyl group in natural folates and glutamate-containing antifolates. Cells deficient in this enzyme are auxothrophic for thymidine and a purine
-
?
additional information
?
-
-
the enzyme catalyzes the formation of polyglutamates from folates and folate antimetabolites in mammalian cells
-
?
additional information
?
-
-
the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
folylpolyglutamate synthesis is not mediated by a single enzyme, but by at least two soluble enzymes which are products of the mac and met-6 loci respectively
-
-
?
additional information
?
-
-
the expression of the enzyme in Escherichia coli and Saccharomyces cerevisiae defective strains restores Escherichia coli cells to methionine and glycine prototrophy on minimal medium, but cannot entirely compensate for the loss of the native homologue in Saccharomyces cerevisiae. The malarial gene encodes a protein carrying dihydrofolate synthetase and folylpolyglutamate synthetase activities, both needed for the de novo folate synthesis
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
met7 mutant strain is a methionine auxotroph and an adenine and thymidine auxotroph when grown in the presence of sulfanilamide. Met7 mutants have a petite phenotype, defective in mitochondrial respiration and unable to grow on glycerol or other nonfermentable substrates as a carbon/energy source. Disruption of met7 causes deficiencies in folate metabolism and loss of both cytoplasmic and mitochondrial enzyme activity. Met7 mutants are complemented by genes that express only cytoplasmic enzyme
-
?
additional information
?
-
-
enzyme catalyzes the addition of glutamate residues to folates and antifolates to form the physiological active coenzymatic forms of the vitamin and more potent anti-folate agents
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
no dihydrofolate synthetase activity
-
-
?
additional information
?
-
-
the enzyme catalyzes the consecutive addition of one or more L-glutamyl moieties to the gamma-carboxyl group in natural folates and glutamate-containing antifolates. Cells deficient in this enzyme are auxothrophic for thymidine and a purine
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.002 - 0.0029
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate
0.00193
(6R)-5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
0.00087
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
0.00096
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.0016
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.00105
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
0.0145
(6R)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate-gamma-L-glutamate
-
-
1
(6RS)-5,6,7,8-tetrahydrofolic acid
-
pH 9.75
0.00106
(6S)-5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
0.00116
(6S)-5,10-dideaza-5,6,7,8-tetrahydropteroyl-L-glutamate-gamma-L-glutamate
-
-
0.0014 - 0.0063
(6S)-5,6,7,8-tetrahydropteroylglutamate
0.075 - 0.122
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate
0.054 - 0.064
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate
0.0022 - 0.014
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.41
3,3-difluoroglutamate
-
reaction with methotrexate + ATP
0.0031 - 0.0054
3,3-difluoromethotrexate
0.0012 - 0.0076
5,10-dideazatetrahydrofolic acid
0.03 - 3.8
5,10-methylene-5,6,7,8-tetrahydrofolic acid
0.01 - 0.204
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
0.0048 - 0.057
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
0.0023
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.00096
5,6,7,8-tetrahydrofolate
-
-
0.00123
5,6,7,8-tetrahydrofolate-Glu2
-
pH 8.7
0.01 - 0.655
5,6,7,8-tetrahydrofolic acid
0.16
5,6,7,8-tetrahydrofolyl-beta-L-Ala-L-Glu
-
L-Glu
-
0.3
5,6,7,8-tetrahydrofolyl-gamma-(L-Glu)2
mutant D154A, pH 9.8
0.074
5,6,7,8-tetrahydropteroyl-(4-aminobutanoyl)-Glu
-
-
0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-Glu
-
L-Glu
0.018 - 0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
0.0021 - 6.8
5,6,7,8-tetrahydropteroyl-Glu
0.0033 - 0.0034
5,6,7,8-tetrahydropteroyl-Glu2
0.0011 - 0.0014
5,6,7,8-tetrahydropteroyl-Glu3
0.0016 - 0.002
5,6,7,8-tetrahydropteroyl-Glu4
0.0027
5,6,7,8-tetrahydropteroyl-Glu5
0.0081 - 0.105
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.013
5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.444
5-methyl-H4-homofolate
-
-
0.0063 - 0.98
7,8-dihydropteroate
0.012
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu
0.022
7,8-dihydropteroyl-(5-aminopentanoyl)-Glu
-
-
0.076
7,8-dihydropteroyl-beta-Ala-Glu
-
-
0.00081 - 0.0086
7,8-dihydropteroyl-Glu
0.0026 - 0.048
7,8-dihydropteroyl-Glu2
0.05
dATP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.0073
dihydroaminopterin
-
-
0.235
glutamic acid
-
pH 8.9, 37ºC, charcoal assay, wild type recombinant enzyme
0.136
ITP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.43 - 350
L-Glutamic acid
0.019 - 0.138
methotrexate
0.063 - 0.074
methotrexate-Glu
0.029
methotrexate-Glu2
-
-
0.011
methotrexate-Glu4
-
methotrexate-Glu5
0.126 - 0.14
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
0.81
pteroyl-(4-aminobutanoyl)-Glu
-
-
1.09
pteroyl-(5-aminopentanoyl)-Glu
-
-
0.69
pteroyl-beta-Ala-Glu
-
-
0.059 - 0.137
pteroyl-Glu
0.962
pteroylglutamate
-
-
0.093
pteroylmonoglutamate
-
-
0.062
pteroylmonoglutamyl-Glu
0.19
UTP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.002
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.0029
(6R)-10-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.0014
(6S)-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.0063
(6S)-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.075
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.122
(6S)-5-formyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.054
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, L1210 isoform
0.064
(6S)-5-methyl-5,6,7,8-tetrahydropteroylglutamate
-
pH 8.9, 37ºC, liver isoform
0.0022
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0037
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0039
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.014
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0031
3,3-difluoromethotrexate
-
-
0.0054
3,3-difluoromethotrexate
-
-
0.0012
5,10-dideazatetrahydrofolic acid
-
pH 8.9, 37ºC, L1210 isoform
0.0076
5,10-dideazatetrahydrofolic acid
-
pH 8.9, 37ºC, liver isoform
0.03
5,10-methylene-5,6,7,8-tetrahydrofolic acid
-
pH 9.75
0.032
5,10-methylene-5,6,7,8-tetrahydrofolic acid
wild-type
0.034
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant S152A, pH 9.8
0.048
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant Y414A, pH 9.8
0.05
5,10-methylene-5,6,7,8-tetrahydrofolic acid
wild-type
0.071
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.071
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.084
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant R15E, pH 9.8
0.11
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant D151A, pH 9.8
0.116
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant S152W, pH 9.8
0.118
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-71 of Lactobacillus casei enzyme, residues 82-93 of Escherichia coli enzyme, and residues 83-428 of Lactobacillus casei enzyme, pH 9.8
0.118
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-71 of Lactobacillus casei enzyme, residues 82-93 of Escherichia coli enzyme, and residues 83-428 of Lactobacillus casei enzyme, pH 9.8
0.23
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant F75A, pH 9.8
0.59
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.59
5,10-methylene-5,6,7,8-tetrahydrofolic acid
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
1.5
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant F121A, pH 9.8
3.8
5,10-methylene-5,6,7,8-tetrahydrofolic acid
mutant T119W, pH 9.8
0.01
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
mutant D151A, pH 9.8
0.204
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
mutant R15E, pH 9.8
0.0048
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.027
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.057
5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.01
5,6,7,8-tetrahydrofolic acid
mutant A155W, pH 9.8
0.0315
5,6,7,8-tetrahydrofolic acid
pH 7.5, wild type enzyme
0.071
5,6,7,8-tetrahydrofolic acid
pH 7.5, E81A mutant
0.094
5,6,7,8-tetrahydrofolic acid
pH 7.5, S412A mutant
0.21
5,6,7,8-tetrahydrofolic acid
pH 7.5, R82A mutant
0.24
5,6,7,8-tetrahydrofolic acid
pH 7.5, K185A mutant
0.34
5,6,7,8-tetrahydrofolic acid
mutant D154A, pH 9.8
0.39
5,6,7,8-tetrahydrofolic acid
mutant T122W, pH 9.8
0.4
5,6,7,8-tetrahydrofolic acid
mutant T122H, pH 9.8
0.448
5,6,7,8-tetrahydrofolic acid
mutant A155H, pH 9.8
0.655
5,6,7,8-tetrahydrofolic acid
pH 7.5, S73A mutant
0.018
5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
-
-
0.16
5,6,7,8-tetrahydropteroyl-beta-Ala-L-Glu
-
L-Glu
-
0.0021
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0035
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0044
5,6,7,8-tetrahydropteroyl-Glu
-
aminopterin
0.006
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.007
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0077
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.009
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.163
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.336
5,6,7,8-tetrahydropteroyl-Glu
-
-
6.8
5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0033
5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.0034
5,6,7,8-tetrahydropteroyl-Glu2
-
-
0.0011
5,6,7,8-tetrahydropteroyl-Glu3
-
5,6,7,8-tetrahydropteroyl-Glu5
0.0014
5,6,7,8-tetrahydropteroyl-Glu3
-
-
0.0016
5,6,7,8-tetrahydropteroyl-Glu4
-
-
0.002
5,6,7,8-tetrahydropteroyl-Glu4
-
-
0.002
5,6,7,8-tetrahydropteroyl-Glu4
-
7,8-dihydropteroyl-Glu
0.002
5,6,7,8-tetrahydropteroyl-Glu4
-
5,6,7,8-tetrahydropteroyl-Glu
0.0027
5,6,7,8-tetrahydropteroyl-Glu5
-
-
0.0027
5,6,7,8-tetrahydropteroyl-Glu5
-
10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
0.0081
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.105
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
-
0.0063
7,8-dihydropteroate
wild-type, pH 9.8
0.0063
7,8-dihydropteroate
wild-type, pH 9.8
0.08
7,8-dihydropteroate
mutant A155W, pH 9.8
0.134
7,8-dihydropteroate
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.134
7,8-dihydropteroate
chimera containing N-terminal residues 1-81 of Escherichia coli enzyme, residues 72-82 of Lactobacillus casei enzyme, and residues 93-422 of Escherichia coli enzyme, pH 9.8
0.14
7,8-dihydropteroate
mutant D154A, pH 9.8
0.15
7,8-dihydropteroate
mutant T122W, pH 9.8
0.485
7,8-dihydropteroate
mutant A155H, pH 9.8
0.98
7,8-dihydropteroate
mutant T122H, pH 9.8
0.012
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu
-
-
0.012
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu
-
pteroyl-Glu4
0.00081
7,8-dihydropteroyl-Glu
-
-
0.0009
7,8-dihydropteroyl-Glu
-
-
0.005
7,8-dihydropteroyl-Glu
-
-
0.0086
7,8-dihydropteroyl-Glu
-
-
0.0026
7,8-dihydropteroyl-Glu2
-
-
0.048
7,8-dihydropteroyl-Glu2
-
-
0.004
aminopterin
-
-
0.0059
aminopterin
-
pH 8.9, 37ºC, HPLC assay, R377A mutant
0.0065
aminopterin
-
pH 8.9, 37ºC, HPLC assay, H338A mutant
0.009
aminopterin
-
pH 8.9, 37ºC, charcoal assay, wild type recombinant enzyme
0.0104
aminopterin
-
pH 8.7
0.011
aminopterin
-
pH 8.9, 37ºC, charcoal assay, K384A mutant
0.013
aminopterin
-
pH 8.9, 37ºC, charcoal assay, C346A mutant
0.0154
aminopterin
-
pH 8.9, 37ºC, L1210 isoform
0.017
aminopterin
-
pH 8.9, 37ºC, charcoal assay, D378A mutant
0.0177
aminopterin
-
pH 8.9, 37ºC, liver isoform
0.025
aminopterin
-
pteroyl-3,3-difluoroglutamate
0.031
aminopterin
-
pH 8.9, 37ºC, charcoal assay, D376A mutant
0.18
aminopterin
-
pH 8.9, 37ºC, HPLC assay, D335A mutant
0.0046
ATP
-
pH 8.9, 37ºC, HPLC assay, R377A mutant
0.01
ATP
-
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.01
ATP
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.016
ATP
-
pH 8.9, 37ºC, HPLC assay, H338A mutant
0.028
ATP
-
pH 8.9, 37ºC, charcoal assay, D376A mutant
0.037
ATP
mutant D154A, pH 9.8 cosubstrate 7,8-dihydropteroate
0.04
ATP
-
pH 8.9, 37ºC, charcoal assay, C346A mutant
0.045
ATP
-
pH 8.9, 37ºC, charcoal assay, K384A mutant
0.046
ATP
-
pH 8.9, 37ºC, charcoal assay, wild type recombinant enzyme
0.054
ATP
wild-type, pH 9.8
0.06
ATP
-
pH 8.9, 37ºC, charcoal assay, D378A mutant
0.065
ATP
mutant A155H, pH 9.8 cosubstrate 7,8-dihydropteroate
0.0665
ATP
-
reaction with 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + Glu
0.07
ATP
-
pH 9.75, temperature conditions not mentioned
0.076
ATP
mutant A155H, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.18
ATP
mutant F75A, pH 9.8
0.23
ATP
mutant D154A, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.23
ATP
mutant Y414A, pH 9.8
0.25
ATP
-
reaction with aminopterin + ATP
0.267
ATP
-
pH 8.9, 37ºC, HPLC assay, D335A mutant
0.555
ATP
mutant A155W, pH 9.8 cosubstrate 7,8-dihydropteroate
0.62
ATP
pH 7.5, S412A mutant
0.87
ATP
pH 7.5, R82A mutant
2.5
ATP
pH 7.5, S73A mutant
2.9
ATP
pH 7.5, K185A mutant
3.4
ATP
pH 7.5, wild type enzyme
3.4
ATP
mutant D151A, pH 9.8
3.4
ATP
wild-type, pH 9.8
5.2
ATP
pH 7.5, E81A mutant
5.7
ATP
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
5.7
ATP
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.332
Glu
-
-
0.333
Glu
-
reaction with 10-formyl-5,6,7,8-tetrahydropteroyl-Glu
0.346
Glu
-
reaction with 5,6,7,8-tetrahydropteroyl-Glu + ATP
0.74
Glu
-
reaction with methotrexate + ATP
1.63
Glu
-
mutant enzyme A447V
1.702
Glu
-
wild type enzyme from cytosol
2.068
Glu
-
wild type enzyme from mitochondrion
2.281
Glu
-
mutant enzyme S457F
5.984
Glu
-
mutant enzyme R424C
0.053
L-Glu
mutant F75A, pH 9.8
0.12
L-Glu
mutant A155H, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.17
L-Glu
mutant A155H, pH 9.8, cosubstrate 7,8-dihydropteroate
0.2
L-Glu
mutant D154A, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.292
L-Glu
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.292
L-Glu
chimera containing N-terminal residues 1-298 of Lactobacillus casei enzyme, residues 288-422 of Escherichia coli enzyme, pH 9.8
0.3
L-Glu
wild-type, pH 9.8
0.47
L-Glu
wild-type, pH 9.8
1.2
L-Glu
-
reaction with aminopterin + ATP
2.7
L-Glu
mutant Y414A, pH 9.8
3.3
L-Glu
mutant D154A, pH 9.8, cosubstrate 5,6,7,8-tetrahydrofolic acid
0.24
L-glutamate
-
pH 8.7
0.41
L-glutamate
pH 7.5, E81A mutant
0.47
L-glutamate
pH 7.5, wild type enzyme
0.85
L-glutamate
pH 7.5, S73A mutant
1
L-glutamate
-
pH 8.5, 37ºC, wild type cell line
2 - 3
L-glutamate
-
pH 8.5, 37ºC, MTXR5 cell subline
2.06
L-glutamate
pH 7.5, R82A mutant
5.06
L-glutamate
pH 7.5, K185A mutant
26.69
L-glutamate
pH 7.5, S412A mutant
0.43
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, K384A mutant
0.52
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, C346A mutant
0.83
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, D378A mutant
2.9
L-Glutamic acid
-
pH 8.9, 37ºC, charcoal assay, D376A mutant
91
L-Glutamic acid
-
pH 8.9, 37ºC, HPLC assay, D335A mutant
140
L-Glutamic acid
-
pH 8.9, 37ºC, HPLC assay, H338A mutant
350
L-Glutamic acid
-
pH 8.9, 37ºC, HPLC assay, R377A mutant
0.019
methotrexate
-
-
0.04
methotrexate
-
pH 8.7
0.0526
methotrexate
-
wild type enzyme from mitochondrion
0.054
methotrexate
-
5-methyl-5,6,7,8-tetrahydrpteroyl-Glu
0.0555
methotrexate
-
wild type enzyme from cytosol
0.0613
methotrexate
-
mutant enzyme S457F
0.0827
methotrexate
-
mutant enzyme A447V
0.0908
methotrexate
-
mutant enzyme R424C
0.1
methotrexate
-
aminopterin
0.063
methotrexate-Glu
-
pH 8.5, 37ºC, wild type cell line
0.074
methotrexate-Glu
-
pH 8.5, 37ºC, MTXR5 cell subline
0.02
methotrexate-Glu3
-
-
0.02
methotrexate-Glu3
-
pteroyl-Glu3
0.126
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
-
pH 8.7
0.14
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
-
-
0.14
N-(p(((2-amino-4-hydroxy-6-pteridinyl)methyl)-amino)benzoyl)glutamic acid
-
-
0.059
pteroyl-Glu
-
-
0.062
pteroylmonoglutamyl-Glu
-
-
0.062
pteroylmonoglutamyl-Glu
-
pteroyl-Glu2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.