Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
relative activity wild-type: 14%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Ala-D-Ala
?
-
relative activity wild-type: 5%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-allo-cystathionine
ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-D-allo-cystathionine
-
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Glu
?
-
relative activity wild-type: 13%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-Lys
?
-
relative activity wild-type: 9%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + D-ornithine
?
-
relative activity wild-type: 8%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + DL-lanthionine
?
-
relative activity wild-type: 82%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + L-Ala
?
-
relative activity wild-type: 12%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + L-allo-cystathionine
ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-L-allo-cystathionine
-
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + L-cystathionine
?
-
relative activity wild-type: 12%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + L-Lys
?
-
relative activity wild-type: 7%
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-lanthionine
ADP + phosphate + UDP-MurNAc-L-Ala-D-Glu-meso-lanthionine
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycyl-gamma-D-glutamyl-L-alanine
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + (2S,6S)-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-(2S,6S)-2,6-diaminoheptanedioate
7.8% activity with (2S,6S)-2,6-diaminoheptanedioate compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + beta-chloro-L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-beta-chloro-L-alanine
15% activity with beta-chloro-L-alanine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + glycine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-glycine
7% activity with glycine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-alanine
highest activity with L-alanine compared to the other amino acids
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-diaminobutyrate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-diaminobutyrate
7.9% activity with L-diaminobutyrate compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-homoserine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-homoserine
10% activity with L-homoserine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-propargylglycine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-propargylglycine
20% activity with L-propargylglycine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-serine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-serine
30% activity with L-serine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + L-vinylglycine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-vinylglycine
58% activity with L-vinylglycine compared to L-alanine
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate + L-alanine
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-seryl-gamma-D-glutamyl-L-alanine
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
GTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
GDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
relative activity wild-type: 30%
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
additional information
?
-
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
r
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
specific for UDP-N-MurNAc-L-Ala-D-Glu and meso-2,6-diaminoheptanedioate
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
r
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
the threedimensional structure of MurE from Mycobacterium leprae is modeled using comparative modeling methods based on the X-ray crystal structure of MurE from Escherichia coli. The docked complexes reveal the amino acids responsible for binding the substrates. Superposition of these complex structures suggests that carboxylic acid group of UDP-Nacetyl muramoyl-glycyl-D-glutamate is positioned in proximity to gamma-phosphate of the ATP to facilitate the formation of acylphosphate intermediate. The orientation of an amino group of meso-diaminopimelic acid facilitates the nucleophilic attack to form the product
-
-
?
ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
relative activity wild-type: 100%
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
30% activity with meso-2,6-diaminoheptanedioate compared to L-alanine
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + D-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-D-lysine
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-lysine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-lysine
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + L-ornithine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-L-ornithine
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
-
r
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
part of bacterial cell-wall peptidoglycan biosynthesis
-
r
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
essential for optimal expression of methicillin resistance
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
-
-
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
catalyzes an early reaction in the biosynthesis of bacterial peptidoglycan
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
the adding activity is not in excess in the cell under normal growth conditions, but their amounts appear adjusted to the requirements of peptidoglycan synthesis
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
enzyme is not essential for growth
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
-
-
-
-
?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + ATP + meso-diaminopimelic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid
-
assay at pH 8.0
-
-
?
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
-
?
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
16% of the activity relative to ATP
-
?
UTP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
UDP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
relative activity wild-type: 17%
-
-
?
additional information
?
-
enzyme substrate specificity, overview. Poor activity with D-alanine, L-lysine, L-ornithine, L-cysteine, L-allylglycine, L-1-aminoethylphosphonic acid, L-alanine amide, L-norvaline and L-alpha-aminobutyric acid. Enzyme-ligand interaction analysis, overview
-
-
?
additional information
?
-
-
enzyme substrate specificity, overview. Poor activity with D-alanine, L-lysine, L-ornithine, L-cysteine, L-allylglycine, L-1-aminoethylphosphonic acid, L-alanine amide, L-norvaline and L-alpha-aminobutyric acid. Enzyme-ligand interaction analysis, overview
-
-
?
additional information
?
-
highly specific in adding meso-diaminopimelic acid to UDP-MurNAc-dipeptide. No substrate: L-Lys, D-Lys, m-Lys, L-Ala, D-Ala, L-Glu, D-Glu, Gly, Gly-Gly, DL-ornithine, N-acetylmuramic acid, UDP-N-acetylmuramoyl pentapeptide. ATP-hydrolysis is an absolute requirement for activity
-
-
?
additional information
?
-
MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP
-
-
?
additional information
?
-
highly specific in adding meso-diaminopimelic acid to UDP-MurNAc-dipeptide. No substrate: L-Lys, D-Lys, m-Lys, L-Ala, D-Ala, L-Glu, D-Glu, Gly, Gly-Gly, DL-ornithine, N-acetylmuramic acid, UDP-N-acetylmuramoyl pentapeptide. ATP-hydrolysis is an absolute requirement for activity
-
-
?
additional information
?
-
MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
additional information
?
-
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
part of bacterial cell-wall peptidoglycan biosynthesis
-
r
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
essential for optimal expression of methicillin resistance
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
catalyzes an early reaction in the biosynthesis of bacterial peptidoglycan
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
the adding activity is not in excess in the cell under normal growth conditions, but their amounts appear adjusted to the requirements of peptidoglycan synthesis
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
enzyme is not essential for growth
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
?
ATP + UDP-N-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate
?
-
enzyme activity is required exclusively for cortical peptidoglycan synthesis, it is absent during vegetative growth, and is synthesized during forespore maturation
-
-
?
additional information
?
-
MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP
-
-
?
additional information
?
-
MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10
D-allo-cystathionine
-
-
0.097
dihydrouridine-5'-diphosphate-MurNAc-L-Ala-D-Glu
-
-
3.9
L-allo-cystathionine
-
-
0.012 - 77
meso-2,6-diaminoheptanedioate
0.069 - 4.8
meso-diaminopimelic acid
0.005 - 0.148
UDP-MurNAc-L-Ala-D-Glu
0.23
UDP-N-acetyl-alpha-D-muramoyl-glycyl-D-glutamate
pH 9.6, 37°C, recombinant His-tagged enzyme
0.16
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
pH 9.6, 37°C, recombinant His-tagged enzyme
0.21
UDP-N-acetyl-alpha-D-muramoyl-L-seryl-D-glutamate
pH 9.6, 37°C, recombinant His-tagged enzyme
0.04
UDP-N-acetylmuramoyl-L-Ala-D-Glu
pH 7.4, 37°C
0.03 - 0.45
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
0.035 - 0.32
UDP-N-MurNAc-L-Ala-D-Glu
0.008
ATP
-
mutant K157A, pH 8.5, 37°C
0.015
ATP
-
mutant R451A, pH 8.5, 37°C
0.017
ATP
-
wild-type, pH 8.5, 37°C
0.021
ATP
-
DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37°C
0.06
ATP
-
mutant N449D, pH 8.5, 37°C
0.1
ATP
-
mutant D392A, pH 8.5, 37°C
0.123
ATP
-
mutant E220A, pH 8.5, 37°C
0.16
ATP
pH 9.6, 37°C, recombinant His-tagged enzyme
1.8
L-alanine
pH 9.6, 37°C, recombinant His-tagged enzyme
64
L-alanine
pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR)
0.012
meso-2,6-diaminoheptanedioate
-
mutant K157A, pH 8.5, 37°C
0.036
meso-2,6-diaminoheptanedioate
-
-
0.04
meso-2,6-diaminoheptanedioate
-
-
0.052
meso-2,6-diaminoheptanedioate
-
mutant E220A, pH 8.5, 37°C
0.065
meso-2,6-diaminoheptanedioate
-
DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37°C
0.078
meso-2,6-diaminoheptanedioate
-
wild-type, pH 8.5, 37°C
0.113
meso-2,6-diaminoheptanedioate
-
mutant D392A, pH 8.5, 37°C
0.13
meso-2,6-diaminoheptanedioate
-
-
0.324
meso-2,6-diaminoheptanedioate
-
mutant N449D, pH 8.5, 37°C
0.473
meso-2,6-diaminoheptanedioate
-
mutant R451A, pH 8.5, 37°C
10
meso-2,6-diaminoheptanedioate
pH 9.6, 37°C, recombinant mutant MurEEr(HDNR/DNPR)
77
meso-2,6-diaminoheptanedioate
pH 9.6, 37°C, recombinant His-tagged enzyme
0.069
meso-diaminopimelic acid
pH 7.4, 37°C
0.14
meso-diaminopimelic acid
-
-
4.8
meso-diaminopimelic acid
-
at 68°C
0.005
UDP-MurNAc-L-Ala-D-Glu
-
mutant K157A, pH 8.5, 37°C, value below
0.005
UDP-MurNAc-L-Ala-D-Glu
-
mutant R451A, pH 8.5, 37°C
0.007
UDP-MurNAc-L-Ala-D-Glu
-
mutant N449D, pH 8.5, 37°C
0.016
UDP-MurNAc-L-Ala-D-Glu
-
mutant D392A, pH 8.5, 37°C
0.019
UDP-MurNAc-L-Ala-D-Glu
-
DELTA1-24 (N-terminal deletion mutant), pH 8.5, 37°C
0.024
UDP-MurNAc-L-Ala-D-Glu
-
wild-type, pH 8.5, 37°C
0.148
UDP-MurNAc-L-Ala-D-Glu
-
mutant E220A, pH 8.5, 37°C
0.03
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
-
0.45
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
at 68°C
0.035
UDP-N-MurNAc-L-Ala-D-Glu
-
-
0.076
UDP-N-MurNAc-L-Ala-D-Glu
-
-
0.32
UDP-N-MurNAc-L-Ala-D-Glu
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D399A
site-directed mutagenesis, inactive mutant
D399N
site-directed mutagenesis, almost inactive mutant
F340A
site-directed mutagenesis, almost inactive mutant
F340Y
site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme
H398A
site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme
H398D
site-directed mutagenesis, the mutant shows highly reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme
N400A
site-directed mutagenesis, the mutant shows reduced activity with substrate L-alanine and meso-2,6-diaminoheptanedioate compared to wild-type enzyme
N400P
site-directed mutagenesis, the mutant shows slightly reduced activity with substrate meso-2,6-diaminoheptanedioate, but increased activity with substrate L-alanine compared to wild-type enzyme
R401A
site-directed mutagenesis, almost inactive mutant
D392A
-
kcat lower than wild-type, Km (meso-2,6-diaminoheptanedioate) and Km (ATP) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by at least partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100%
DELTA1-24
-
N-terminal deletion mutant, kcat comparable to wild-type, Km values comparable to wild-type, mutant shows similar results to the wild-type with all the nucleotides, UDP-MurNAc peptides and amino acids
E220A
-
kcat lower than wild-type, Km (ATP) and Km (UDP-MurNAc-L-Ala-D-Glu), Km (meso-2,6-diaminoheptanedioate) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by atleast partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100%
K157A
-
kcat lower than wild-type, Km (meso-2,6-diaminoheptanedioate), Km (UDP-MurNAc-L-Ala-D-Glu) and Km (ATP) lower than wild-type, mutant shows hydrolysis of ATP uncoupled from catalysis. The ATP hydrolysis rate is enhanced by at least partial occupation of the uridine nucleotide dipeptide binding site, mutant shows very high activity with all the amino acids, uridine sugar precursors and nucleotides tested compared to wild type and moreover, reaching up to 100%
N449D
-
kcat lower than wild-type, Km (ATP) and Km (meso-2,6-diaminoheptanedioate) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, mutant shows similar results to the wild-type with all the nucleotides, UDP-MurNAc peptides and amino acids, except D-Lys, which shows a slightly higher activity for mutant N449D
R451A
-
kcat lower than wild-type, Km (ATP) comparable to wild-type, Km (meso-2,6-diaminoheptanedioate) higher than wild-type, Km (UDP-MurNAc-L-Ala-D-Glu) lower than wild-type, significant activity in the presence of L-Lys, D-Lys, DL-ornithine and D-Glu for mutant R451A with similar activities as wild type for all the nucleotides and UDP-MurNAc peptides tested
additional information
construction of an enzyme mutant MurEEr(HDNR/DNPR) with exchange of the HDNR morif to a DNPR motif, which is characteristic of meso-diaminopimelate-adding enzymes. The mutant shows increased activity with substrate meso-2,6-diaminoheptanedioate and reduced activity with substrate L-alanine
additional information
-
construction of an enzyme mutant MurEEr(HDNR/DNPR) with exchange of the HDNR morif to a DNPR motif, which is characteristic of meso-diaminopimelate-adding enzymes. The mutant shows increased activity with substrate meso-2,6-diaminoheptanedioate and reduced activity with substrate L-alanine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Linnett, P.E.; Tipper, D.J.
Cell wall polymers of Bacillus sphaericus: activities of enzymes involved in peptidoglycan precursor synthesis during sporulation
J. Bacteriol.
120
342-354
1974
Lysinibacillus sphaericus, Lysinibacillus sphaericus 9602
brenda
Abo-Ghalia, M.; Michaud, C.; Blanot D.; van Heijenoort, J.
Specificity of the uridine-diphosphate-N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate synthetase from Escherichia coli
Eur. J. Biochem.
153
81-87
1985
Escherichia coli
brenda
Mizuno, Y.; Ito, E.
Diaminopimelic-adding enzyme (Bacillus cereus)
Methods Enzymol.
17B
150-154
1971
Bacillus cereus
-
brenda
Mizuno, Y.; Ito, E.
Purification and properties of uridine diphosphate N-acetylmuramyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase
J. Biol. Chem.
243
2665-2672
1968
Bacillus cereus
brenda
Tipper, D.J.; Linnett, P.E.
Distribution of peptidoglycan synthetase activities between sporangia and forespores in sporulating cells of Bacillus sphaericus
J. Bacteriol.
126
213-221
1976
Lysinibacillus sphaericus, Lysinibacillus sphaericus 9602
brenda
Mengin-Lecreulx, D.; van Heijenoort, J.; Park, J.T.
Identification of the mpl gene encoding UDP-N-acetylmuramate:L-alanyl-gamma-glutamyl-meso-diaminopimelate ligase in Escherichia coli and its role in recycling of cell wall peptidoglycan
J. Bacteriol.
178
5347-5352
1996
Escherichia coli
brenda
Banerjee, R.V.; Shane, B.; McGuire, J.J.; Coward, J.K.
Dihydrofolate synthetase and folylpolyglutamate synthetase: direct evidence for intervention of acyl phosphate intermediates
Biochemistry
27
9062-9070
1988
Escherichia coli
brenda
le Roux, P.; Auger, G.; van Heijenoort, J.; Blanot, D.
Synthesis of new peptide inhibitors of the meso-diaminopimelate-adding enzyme
Eur. J. Med. Chem.
27
899-907
1992
Escherichia coli
-
brenda
Abo-Ghalia, M.; Flegel, M.; Blanot, D.; van Heijenoort, J.
Synthesis of inhibitors of the meso-diaminopimelate-adding enzyme from Escherichia coli
Int. J. Pept. Protein Res.
32
208-222
1988
Escherichia coli
brenda
Mengin-Lecreulx, D.; Blanot, D.; van Heijenoort, J.
Replacement of diaminopimelic acid by cystathionine or lanthionine in the peptidoglycan of Escherichia coli
J. Bacteriol.
176
4321-4327
1994
Escherichia coli
brenda
Michaud, C.; Mengin-Lecreulx, D.; van Heijenoort, J.; Blanot, D.
Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase from Escherichia coli
Eur. J. Biochem.
194
853-861
1990
Escherichia coli
brenda
Mengin-Lecreulx, D.; Flouret, B.; Van Heijenoort, J.
Cytoplasmic steps of peptidoglycan synthesis in Escherichia coli
J. Bacteriol.
151
1109-1117
1982
Escherichia coli
brenda
Reusch, V.M.; Hale, S.G.; Hurly, B.J.
Levels of cell wall enzymes in endospores and vegetative cells of Bacillus subtilis
J. Bacteriol.
152
1147-1153
1982
Bacillus subtilis
brenda
Azzolina, B.A.; Yuan, X.; Anderson, M.S.; El-Sherbeini, M.
The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: Cloning, production, and purification of active enzymes
Protein Expr. Purif.
21
393-400
2001
Pseudomonas aeruginosa
brenda
Dementin, S.; Bouhss, A.; Auger, G.; Parquet, C.; Mengin-Lecreulx, D.; Dideberg, O.; van Heijenoort, J.; Blanot, D.
Evidence of a functional requirement for a carbamoylated lysine residue in MurD, MurE and MurF synthetases as established by chemical rescue experiments
Eur. J. Biochem.
268
5800-5807
2001
Escherichia coli
brenda
Gordon, E.; Flouret, B.; Chantalat, L.; van Heijenoort, J.; Mengin-Lecreulx, D.; Dideberg, O.
Crystal structure of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase from Escherichia coli
J. Biol. Chem.
276
10999-11006
2001
Escherichia coli (P22188)
brenda
Ludovice, A.M.; Wu, S.W.; de Lencastre, H.
Molecular cloning and DNA sequencing of the Staphylococcus aureus UDP-N-acetylmuramyl tripeptide synthetase (murE) gene, essential for the optimal expression of methicillin resistance
Microb. Drug Resist.
4
85-90
1998
Staphylococcus aureus
brenda
Herve, M.; Boniface, A.; Gobec, S.; Bianot, D.; Mengin-Lecreulx, D.
Biochemical characterization and physiological properties of Escherichia coli UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
J. Bacteriol.
189
3987-3995
2007
Escherichia coli
brenda
Boniface, A.; Bouhss, A.; Mengin-Lecreulx, D.; Blanot, D.
The MurE synthetase from Thermotoga maritima is endowed with an unusual D-lysine adding activity
J. Biol. Chem.
281
15680-15686
2006
Thermotoga maritima
brenda
Humljan, J.; Kotnik, M.; Boniface, A.; Solmajer, T.; Urleb, U.; Blanot, D.; Gobec, S.
A new approach towards peptidosulfonamides: synthesis of potential inhibitors of bacterial peptidoglycan biosynthesis enzymes MurD and MurE
Tetrahedron
62
10980-10988
2006
Staphylococcus aureus
-
brenda
Paradis-Bleau, C.; Lloyd, A.; Sanschagrin, F.; Maaroufi, H.; Clarke, T.; Blewett, A.; Dowson, C.; Roper, D.I.; Bugg, T.D.; Levesque, R.C.
Pseudomonas aeruginosa MurE amide ligase: enzyme kinetics and peptide inhibitor
Biochem. J.
421
263-272
2009
Pseudomonas aeruginosa
brenda
Guzman, J.D.; Wube, A.; Evangelopoulos, D.; Gupta, A.; Huefner, A.; Basavannacharya, C.; Rahman, M.M.; Thomaschitz, C.; Bauer, R.; McHugh, T.D.; Nobeli, I.; Prieto, J.M.; Gibbons, S.; Bucar, F.; Bhakta, S.
Interaction of N-methyl-2-alkenyl-4-quinolones with ATP-dependent MurE ligase of Mycobacterium tuberculosis: antibacterial activity, molecular docking and inhibition kinetics
J. Antimicrob. Chemother.
66
1766-1772
2011
Mycobacterium tuberculosis
brenda
Shanmugam, A.; Natarajan, J.
Comparative modeling of UDP-N-acetylmuramoyl-glycyl-D-glutamate-2,6-diaminopimelate ligase from Mycobacterium leprae and analysis of its binding features through molecular docking studies
J. Mol. Model.
18
115-125
2012
Mycobacterium leprae, Mycobacterium leprae (O69557)
brenda
Basavannacharya, C.; Moody, P.R.; Munshi, T.; Cronin, N.; Keep, N.H.; Bhakta, S.
Essential residues for the enzyme activity of ATP-dependent MurE ligase from Mycobacterium tuberculosis
Protein Cell
1
1011-1022
2010
Mycobacterium tuberculosis
brenda
Basavannacharya, C.; Robertson, G.; Munshi, T.; Keep, N.; Bhakta, S.
ATP-dependent MurE ligase in Mycobacterium tuberculosis: Biochemical and structural characterisation
Tuberculosis
90
16-24
2010
Mycobacterium tuberculosis (P9WJL3), Mycobacterium tuberculosis H37Rv (P9WJL3)
brenda
Munshi, T.; Gupta, A.; Evangelopoulos, D.; Guzman, J.D.; Gibbons, S.; Keep, N.H.; Bhakta, S.
Characterisation of ATP-dependent Mur ligases involved in the biogenesis of cell wall peptidoglycan in Mycobacterium tuberculosis
PLoS ONE
8
e60143
2013
Mycobacterium tuberculosis (P9WJL3), Mycobacterium tuberculosis H37Rv (P9WJL3)
brenda
Patin, D.; Turk, S.; Barreteau, H.; Mainardi, J.L.; Arthur, M.; Gobec, S.; Mengin-Lecreulx, D.; Blanot, D.
Unusual substrate specificity of the peptidoglycan MurE ligase from Erysipelothrix rhusiopathiae
Biochimie
121
209-218
2016
Erysipelothrix rhusiopathiae (E7FUC9), Erysipelothrix rhusiopathiae
brenda