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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
gamma-L-Glu-L-alpha-aminobutyrylglycine + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP
?
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gamma-L-Glu-L-alpha-aminobutyrylglycine i.e. ophthalmic acid
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?
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP + H2O
N1,N8-bis-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate + NH3
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
glutathione + N8-acetylspermidine + ATP
N1-glutathionyl-N8-acetylspermidine + ADP + phosphate
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-
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?
glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
additional information
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
trypanothione synthase overall reaction
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
trypanothione synthase overall reaction
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase, overview
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids
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?
2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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trypanothione synthase overall reaction
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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trypanothione synthase overall reaction, trypanothione metabolism overview
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase
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gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants
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?
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
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the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione
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gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP + H2O
N1,N8-bis-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate + NH3
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-
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?
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP + H2O
N1,N8-bis-(gamma-L-Glu-L-Cys-Gly)-spermidine + ADP + phosphate + NH3
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N1-glutathionylspermidine and N8-glutathionylspermidine are intermediates
?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
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-
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?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
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-
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?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
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trivial name trypanothione
?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
multifunctional enzyme having also glutathionylspermidine synthetase, glutathionylaminopropylcadaverine synthetase, homotrypanothione synthetase and a weak amidase activity
trivial name trypanothione
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
dynamic interactions of remote domains in substrate binding and catalysis
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?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
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?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9
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glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9
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glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
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?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
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?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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-
-
-
?
glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
-
-
?
glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
ATP in form of MgATP2-
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
ATP in form of MgATP2-
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
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-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9
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-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9
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-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
ATP in form of MgATP2-
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-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
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-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
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-
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
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?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
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?
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
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reaction of EC 6.3.1.8, step one
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?
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
-
reaction of EC 6.3.1.8, step one
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?
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
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reaction of EC 6.3.1.8, step one
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?
N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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step two
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N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
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N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
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N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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?
N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two, N8-glutathionylspermidine is the favoured substrate
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?
N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
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N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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step two
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additional information
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the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8
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additional information
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the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8
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additional information
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the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and exhibits amidase and marginal ATPase activities
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additional information
?
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the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and exhibits amidase and marginal ATPase activities
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additional information
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trypanothione synthetase synthesizes trypanothione from glutathione and spermidine
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additional information
?
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trypanothione synthetase synthesizes trypanothione from glutathione and spermidine
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additional information
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trypanothione synthetase synthesizes trypanothione from glutathione and spermidine
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additional information
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phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome
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additional information
?
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phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome
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additional information
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the bifunctional trypanothione synthetase-amidase catalyzes biosynthesis and hydrolysis of the glutathione-spermidine adduct trypanothione, the principal intracellular thiolredox metabolite in parasitic trypanosomatids
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additional information
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structure modelling of substrate binding to the active site, overview
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additional information
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the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction
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additional information
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in pathogenic trypanosomes, trypanothione synthetase catalyzes the synthesis of both glutathionylspermidine and trypanothione (bis(glutathionyl)spermidine). Trypanothione is synthesized from two molecules of GSH that are covalently bridged by a spermidine in two ATP-dependent reactions. The enzyme also shows trypanothione amidase activity catalyzed at a second distinct active site
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additional information
?
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in pathogenic trypanosomes, trypanothione synthetase catalyzes the synthesis of both glutathionylspermidine and trypanothione (bis(glutathionyl)spermidine). Trypanothione is synthesized from two molecules of GSH that are covalently bridged by a spermidine in two ATP-dependent reactions. The enzyme also shows trypanothione amidase activity catalyzed at a second distinct active site
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
additional information
?
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2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
trypanothione synthase overall reaction
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-
?
2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase, overview
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?
2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
important reaction in defense against chemical and oxidant stress, thiol redox homeostasis, ribonucleotide metabolism, and drug resistance in parasitic kinetoplastids
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-
?
2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
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trypanothione synthase overall reaction, trypanothione metabolism overview
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?
2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase
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?
2 glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
overall reaction, the enzyme catalyzes the formation of N1,N8-bis(glutathionyl)spermidine in two reaction steps, exhibiting activity of EC 6.3.1.8, glutathionylspermidine synthase, and 6.3.1.9, trypanothione synthase
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?
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
synthesis of trypanothione, which is involved in maintaining intracellular thiol redox and in defense against oxidants
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-
?
gamma-L-Glu-L-Cys-Gly + N1-(gamma-Glu-L-Cys-Gly)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
-
the enzyme is involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione
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?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
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-
-
?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
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-
-
?
gamma-L-glutamyl-L-cysteinyl-glycine + N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ATP
N1,N8-bis-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate
-
trivial name trypanothione
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
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?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9
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?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
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-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
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-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
?
glutathione + glutathionylspermidine + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
-
-
-
?
glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
-
-
?
glutathione + spermidine + 2 ATP
N1,N8-bis(glutathionyl)spermidine + 2 ADP + 2 phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionylspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
trypanothione synthase catalyzes both the reaction of glutathionyspermidine synthase, EC 6.3.1.8, and of trypanothione synthase, EC 6.3.1.9, in Trypanosoma brucei
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
?
glutathione + spermidine + ATP
glutathionylspermidine + ADP + phosphate
-
-
-
-
?
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
-
reaction of EC 6.3.1.8, step one
-
-
?
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
-
reaction of EC 6.3.1.8, step one
-
-
?
glutathione + spermidine + ATP
N1-glutathionylspermidine + N8-glutathionylspermidine + ADP + phosphate
-
reaction of EC 6.3.1.8, step one
-
-
?
N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
-
-
?
N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
-
-
?
N1-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
-
-
?
N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two, N8-glutathionylspermidine is the favoured substrate
-
-
?
N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
-
-
?
N8-glutathionylspermidine + glutathione + ATP
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
step two
-
-
?
additional information
?
-
the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8
-
-
?
additional information
?
-
-
the trypanothione synthase also catalyzes the reaction of glutathionylspermidine synthase, EC 6.3.1.8
-
-
?
additional information
?
-
trypanothione synthetase synthesizes trypanothione from glutathione and spermidine
-
-
?
additional information
?
-
-
trypanothione synthetase synthesizes trypanothione from glutathione and spermidine
-
-
?
additional information
?
-
trypanothione synthetase synthesizes trypanothione from glutathione and spermidine
-
-
?
additional information
?
-
phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome
-
-
?
additional information
?
-
-
phylogenetic analysis implies that TryS will replace the glutathionylspermidine synthase/trypanothione synthase complex in Leishmia major loosing the redundant GspS pseudogene from its genome
-
-
?
additional information
?
-
the bifunctional trypanothione synthetase-amidase catalyzes biosynthesis and hydrolysis of the glutathione-spermidine adduct trypanothione, the principal intracellular thiolredox metabolite in parasitic trypanosomatids
-
-
?
additional information
?
-
-
the enzyme is important for several functions, e.g. for detoxification of H2O2 and organic hyperoxids, and ribonucleotide reduction
-
-
?
additional information
?
-
-
in pathogenic trypanosomes, trypanothione synthetase catalyzes the synthesis of both glutathionylspermidine and trypanothione (bis(glutathionyl)spermidine). Trypanothione is synthesized from two molecules of GSH that are covalently bridged by a spermidine in two ATP-dependent reactions. The enzyme also shows trypanothione amidase activity catalyzed at a second distinct active site
-
-
?
additional information
?
-
-
in pathogenic trypanosomes, trypanothione synthetase catalyzes the synthesis of both glutathionylspermidine and trypanothione (bis(glutathionyl)spermidine). Trypanothione is synthesized from two molecules of GSH that are covalently bridged by a spermidine in two ATP-dependent reactions. The enzyme also shows trypanothione amidase activity catalyzed at a second distinct active site
-
-
?
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(Z)-8-(2-(2,4-dihydroxybenzylidene)hydrazinyl)-7-(2-hydroxy-3-phenoxy propyl)-1,3-dimethyl-1H-purine-2,6(3H,7H)-dione
88.5% activity remaining
1,3,5-trimethyl-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]-1H-pyrazole-4-sulfonamide
-
-
1,4,7-trioxo-1lambda5,4lambda5,7lambda5-benzo[1,2-c:3,4-c':5,6-c'']tris[1,2,5]oxadiazole
64.4% activity remaining
1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
83.5% activity remaining
1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one
1-(3-(1-isobutyl-1H-pyrazol-4-yl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(3-(1H-indol-4-yl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(3-(3-chlorophenyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(3-(3-fluorophenyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(3-(3-pyridyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(3-(4-chlorophenyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(3-(furan-2-yl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(3-phenyl-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
-
-
1-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)-3,3-dimethylbutan-2-one
-
-
1-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)butan-2-one
-
-
1-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)propan-2-one
-
-
1-(5-(3,5-difluorophenyl)-2H-tetrazol-2-yl)-3,3-dimethylbutan-2-one
-
-
1-(5-(3-fluorophenyl)-2H-tetrazol-2-yl)-3,3-dimethylbutan-2-one
-
-
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-hydroxy-3-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(3,4-dimethoxybenzoyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one
1-(tert-butylsulfonylmethyl)-3-(3-fluorophenyl)-1H-indazole
-
-
1-[3-(3-fluorophenyl)-1H-indazol-1-yl]-3,3-dimethylbutan-2-one
-
i.e. DDD85811, inhibits growth of bloodstream Trypanosoma brucei in vitro
1-[5-(3-chloro-4-methylphenyl)-2H-tetrazol-2-yl]-3,3-dimethylbutan-2-one
-
-
2,4-dimethyl-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]-1,3-thiazole-5-sulfonamide
-
-
2,4-dimethyl-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]thiophene-3-sulfonamide
-
-
2,6-dichloro-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
-
-
2,6-dichloro-N-[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]benzenesulfonamide
-
-
2,6-difluoro-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
-
-
2,6-dimethoxy-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]benzenesulfonamide
-
-
2-((tert-butylsulfonyl)methyl)-4-(2-fluorophenyl)thiazole
-
-
2-((tert-butylsulfonyl)methyl)-4-(2-methylphenyl)thiazole
-
-
2-((tert-butylsulfonyl)methyl)-4-(3-chlorophenyl)thiazole
-
-
2-((tert-butylsulfonyl)methyl)-4-(3-methoxyphenyl)thiazole
-
-
2-((tert-butylsulfonyl)methyl)-4-(phenyl)thiazole
-
-
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-1-(piperidin-1-yl)ethanone
-
-
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-N,N-dimethylacetamide
-
-
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-N-(2-(4-methylpiperazin-1-yl)ethyl)acetamide
-
-
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-N-(3-(4-methylpiperazin-1-yl)propyl)acetamide
-
-
2-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)-N,N-dimethylacetamide
-
-
2-(5-(3,5-difluorophenyl)-2H-tetrazol-2-yl)-1-phenylethanone
-
-
2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide
2-(tert-butylsulfonylmethyl)-4-(3-trifluoromethylphenyl)thiazole
-
-
2-methyl-N-((5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl)propane-2-sulfonamide
-
-
2-[3-(3-fluorophenyl)-1H-indazol-1-yl]-1-(piperidin-1-yl)ethanone
-
i.e. DDD86243, inhibits growth of bloodstream Trypanosoma brucei in vitro. Exposure to DDD86243 at 2 x EC50 for 72 h decreases intracellular trypanothione levels to less than 10% of wild type, with a corresponding 5fold increase in the precursor metabolite, glutathione
3,3-dimethyl-1-(3-(1-methyl-1H-indol-4-yl)-1H-indazol-1-yl)butan-2-one
-
-
3,3-dimethyl-1-(3-(naphthalen-2-yl)-1H-indazol-1-yl)butan-2-one
-
-
3,3-dimethyl-1-(3-(pyridin-4-yl)-1H-indazol-1-yl)butan-2-one
-
-
3,3-dimethyl-1-(3-(thiophen-3-yl)-1H-indazol-1-yl)butan-2-one
-
-
3,3-dimethyl-1-(5-phenyl-2H-tetrazol-2-yl)butan-2-one
-
-
3,3-dimethyl-1-[5-[3-(trifluoromethyl)phenyl]-2H-tetrazol-2-yl]butan-2-one
-
-
3,3-dimethyl-1-[5-[4-(trifluoromethyl)phenyl]-2H-tetrazol-2-yl]butan-2-one
-
-
3,5-dichloro-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
-
-
3-((3-(3-fluorophenyl)-1H-indazol-1-yl)methyl)-5-methylisoxazole
-
-
3-((3-(3-fluorophenyl)-1H-indazol-1-yl)methyl)-5-phenylisoxazole
-
-
3-(3-fluorophenyl)-1-(pyridin-2-ylmethyl)-1H-indazole
-
-
3-(3-fluorophenyl)-1-(pyridin-3-ylmethyl)-1H-indazole
-
-
3-(tert-butylsulfonylmethyl)-1-(3-fluorophenyl)-1H-indazole
-
-
3-(tert-butylsulfonylmethyl)-1-phenyl-1H-indazole
-
-
4-((3-(3-fluorophenyl)-1H-indazol-1-yl)methyl)-2,5-dimethyloxazole
-
-
4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-1-(6-methoxy-1,3-benzothiazol-2-yl)-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1-(6-methyl-1,3-benzothiazol-2-yl)-1,5-dihydro-2H-pyrrol-2-one
83.9% activity remaining
4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid
above 100 mM
4-acetyl-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
-
-
4-methoxy-N-[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]benzenesulfonamide
-
-
4-methoxy-N-[[3-(2-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]benzenesulfonamide
-
-
4-methyl-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
-
-
5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole
6-fluoro-3-oxo-4,5-didehydro-2,1,3lambda5-benzoxadiazole
84.1% activity remaining
ammonium sulfate
almost complete inhibition at 500 mM
betulin
competitive inhibition
buthionine sulfoximine
-
-
conessine
competitive allosteric inhibition
DL-(S,R)-buthionine sulfoximine
-
DL-(S,R)-BSO, a multitarget inhibitor of trypanothione synthesis, in vivo effects on nonthiol-supplied epimastigote cells, on Cys-supplied epimastigote cells, and on GSH-supplied epimastigote cells, overview. Inhibition of gamma-glutamylcysteine synthetase and trypanothione synthetase. Wild-type Trypanosoma brucei cells are not restored by supplementing with 0.08 mM GSH. The the L-(S,R)-BSO stereoisomer is more potent that the the D-(S,R)-BSO. Inhibitor docking analysis, binding structure
KCl
approx. 50% inhibition at 500 mM
methyl (3-phenyl-1H-indazol-1-yl)acetate
-
-
N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
N,N-dibenzyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N,N-diethyl-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
-
-
N,N-diethyl-2-(3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl)acetamide
-
-
N-(1-phenylethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-(2-(dimethylamino)ethyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
-
-
N-(2-methoxyethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-(2-phenylethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-(3-(1H-imidazol-1-yl)propyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
-
-
N-(3-(dimethylamino)propyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
-
-
N-(4-chlorobenzyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
-
-
N-(4-chlorobenzyl)-2-(3-(trifluoromethyl)-4,5,6,7-tetrahydro-1Hindazol-1-yl)acetamide
-
-
N-(4-[methyl[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]sulfamoyl]phenyl)acetamide
-
-
N-(4-[[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]sulfamoyl]phenyl)acetamide
-
-
N-(butan-2-yl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-(thiophen-2-ylmethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-cyclohexyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-methyl-(3-(dimethylamino)propyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
-
-
N-phenyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-propyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
-
-
N-[(2-phenyl-1,3-thiazol-4-yl)methyl]naphthalene-2-sulfonamide
-
-
N-[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]benzenesulfonamide
-
-
N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]naphthalene-1-sulfonamide
-
-
N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]thiophene-2-sulfonamide
-
-
N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide
N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea
N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea
N-[4-([[3-(2-chlorophenyl)-1,2,4-oxadiazol-5-yl]methyl]sulfamoyl)phenyl]acetamide
-
-
N-[4-([[3-(2-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]sulfamoyl)phenyl]acetamide
-
-
N-[[3-(2-chlorophenyl)-1,2,4-oxadiazol-5-yl]methyl]benzenesulfonamide
-
-
N-[[3-(2-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]benzenesulfonamide
-
-
N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine
N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine
N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine
N1,N3-bis[(4-methoxyphenyl)methyl]propane-1,3-diamine
-
N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine
N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine
N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine
N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine
N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine
prochlorperazine
-
inhibitor DDD66604, binding mode and structure, overview
tert-butyl-4-(2-(2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamido)ethyl)piperazine-1-carboxylate
-
-
tomatine
competitive inhibition versus spermidine, uncompetitive inhibition with respect to ATP and GSH
trypanothione
-
competitive feedback inhibition
uvaol
competitive allosteric inhibition
[3-[4-([6-(3,4-dimethoxyphenyl)-7-[(ethylcarbamothioyl)amino]pyrido[2,3-d]pyrimidin-2-yl]amino)phenyl]propyl]molybdenum(1+)
81.3% activity remaining
1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one
83.5% activity remaining
1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one
90.8% activity remaining
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-hydroxy-3-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
85.3% activity remaining
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-hydroxy-3-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
97.4% activity remaining
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
82.1% activity remaining
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
98.7% activity remaining
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(3,4-dimethoxybenzoyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one
66.0% activity remaining
1-(6-chloro-1,3-benzothiazol-2-yl)-4-(3,4-dimethoxybenzoyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one
99.6% activity remaining
2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide
-
2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide
59.0% activity remaining
2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide
55.5% activity remaining
4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-1-(6-methoxy-1,3-benzothiazol-2-yl)-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
77.9% activity remaining
4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-1-(6-methoxy-1,3-benzothiazol-2-yl)-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one
97.6% activity remaining
5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole
-
5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole
7.3% activity remaining
5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole
63.5% activity remaining
glutathione
substrate inhibition
glutathione
substrate inhibition
glutathione
-
substrate inhibition
N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
-
N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
24.3% activity remaining
N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
61.3% activity remaining
N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
-
N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
25.3% activity remaining
N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
52.2% activity remaining
N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide
i.e. FS-554, N5-substituted paullone
N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide
i.e. FS-554
N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide
i.e. FS-554
N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide
i.e. FS-554, 40.5% activity remaining
N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea
-
N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea
20.9% activity remaining
N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea
65.4% activity remaining
N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea
-
N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea
48.7% activity remaining
N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea
32.3% activity remaining
N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine
-
N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine
51.1% activity remaining
N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine
53.5% activity remaining
N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine
-
N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine
47.5% activity remaining
N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine
30.8% activity remaining
N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine
-
N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine
42.4% activity remaining
N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine
23.8% activity remaining
N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine
-
N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine
50.9% activity remaining
N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine
25.6% activity remaining
N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine
-
N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine
37.9% activity remaining
N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine
26.6% activity remaining
N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine
-
N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine
61.2% activity remaining
N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine
17.0% activity remaining
N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine
-
N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine
48.9% activity remaining
N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine
33.4% activity remaining
N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine
-
N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine
55.6% activity remaining
N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine
28.9% activity remaining
additional information
limited proteolysis with trypsin or factor Xa result in cleavage of wild-type and mutant enzymes at R553 followed by loss of activity, cleavage of wild-type and mutant R553Q can be delayed by presence of substrates
-
additional information
-
limited proteolysis with trypsin or factor Xa result in cleavage of wild-type and mutant enzymes at R553 followed by loss of activity, cleavage of wild-type and mutant R553Q can be delayed by presence of substrates
-
additional information
screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview. No inhibition by [3-[4-([6-(3,4-dimethoxyphenyl)-7-[(ethylcarbamothioyl)amino]pyrido[2,3-d]pyrimidin-2-yl]amino)phenyl]propyl]molybdenum(1+) and 1,4,7-trioxo-1lambda5,4lambda5,7lambda5-benzo[1,2-c:3,4-c':5,6-c'']tris[1,2,5]oxadiazole
-
additional information
-
screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview. No inhibition by [3-[4-([6-(3,4-dimethoxyphenyl)-7-[(ethylcarbamothioyl)amino]pyrido[2,3-d]pyrimidin-2-yl]amino)phenyl]propyl]molybdenum(1+) and 1,4,7-trioxo-1lambda5,4lambda5,7lambda5-benzo[1,2-c:3,4-c':5,6-c'']tris[1,2,5]oxadiazole
-
additional information
-
the enzyme mediates resistance to H2O2 and organic hyperoxids, and to trypanocidic drugs
-
additional information
-
synthesis and inhibitory potencies of indazole analogue inhibitors, overview
-
additional information
screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview. No effect by 8-[(2E)-2-[(2,4-dihydroxyphenyl)methylidene]hydrazinyl]-7-(2-hydroxy-3-phenoxypropyl)-1,3-dimethyl-3,7-dihydro-1H-purine-2,6-dione and J31
-
additional information
screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview
-
additional information
-
screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview
-
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0.914
gamma-L-Glu-L-Cys-Gly
-
-
0.032 - 0.407
glutathione
0.04 - 0.48
glutathionylspermidine
0.19
GSH
pH 8.0, with N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine
0.066
N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine
pH 8.0
0.012 - 0.032
N1-glutathionylspermidine
0.007 - 0.032
N8-glutathionylspermidine
additional information
additional information
-
0.0066
ATP
-
recombinant enzyme, pH 8.0, 25°C, HEPES buffer, with spermidine
0.01
ATP
-
recombinant enzyme, pH 8.0, 37°C, HEPES buffer, with spermidine
0.012
ATP
-
recombinant enzyme, pH 7.0, 37°C, phosphate buffer, with glutathionylspermidine
0.0142
ATP
pH 8.0, 25°C, recombinant enzyme
0.018
ATP
-
recombinant enzyme, pH 7.0, 37°C, phosphate buffer, with spermidine
0.032
glutathione
-
recombinant enzyme, pH 8.0, 25°C, HEPES buffer, with spermidine
0.0332
glutathione
pH 8.0, 25°C, recombinant enzyme
0.034
glutathione
-
recombinant enzyme, pH 7.0, 37°C, phosphate buffer, with glutathionylspermidine
0.045
glutathione
-
recombinant enzyme, pH 8.0, 37°C, HEPES buffer, with spermidine
0.069
glutathione
-
recombinant enzyme, pH 7.0, 37°C, phosphate buffer, with spermidine
0.089
glutathione
pH 7.7, 25°C, recombinant trypanothione synthase
0.407
glutathione
pH 7.2, 25°C, recombinant trypanothione synthase
0.04
glutathionylspermidine
pH 7.7, 25°C, recombinant trypanothione synthase
0.48
glutathionylspermidine
pH 7.2, 25°C, recombinant trypanothione synthase
0.063
MgATP2-
pH 7.7, 25°C, recombinant trypanothione synthase
0.222
MgATP2-
pH 7.2, 25°C, recombinant trypanothione synthase
0.012
N1-glutathionylspermidine
-
recombinant enzyme, pH 8.0, 25°C, HEPES buffer
0.016
N1-glutathionylspermidine
-
recombinant enzyme, pH 8.0, 37°C, HEPES buffer
0.02
N1-glutathionylspermidine
-
-
0.032
N1-glutathionylspermidine
-
recombinant enzyme, pH 7.0, 37°C, phosphate buffer
0.007
N8-glutathionylspermidine
-
-
0.012
N8-glutathionylspermidine
-
recombinant enzyme, pH 8.0, 25°C, HEPES buffer
0.016
N8-glutathionylspermidine
-
recombinant enzyme, pH 8.0, 37°C, HEPES buffer
0.032
N8-glutathionylspermidine
-
recombinant enzyme, pH 7.0, 37°C, phosphate buffer
0.092
spermidine
-
recombinant enzyme, pH 8.0, 25°C, HEPES buffer
0.139
spermidine
-
recombinant enzyme, pH 8.0, 37°C, HEPES buffer
0.1396
spermidine
pH 8.0, 25°C, recombinant enzyme
0.687
spermidine
-
recombinant enzyme, pH 7.0, 37°C, phosphate buffer
0.94
spermidine
pH 7.7, 25°C, recombinant trypanothione synthase
additional information
additional information
hyperbolic kinetics, steady-state kinetics
-
additional information
additional information
-
hyperbolic kinetics, steady-state kinetics
-
additional information
additional information
steady-state kinetics for the multiple enzyme activities, Dalziel equation, overview
-
additional information
additional information
-
steady-state kinetics for the multiple enzyme activities, Dalziel equation, overview
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten kinetics, simple hyperbolic kinetics with fixed glutathione concentration and with other substrates limiting Km values for Mg2+
-
additional information
additional information
-
Michaelis-Menten kinetics, simple hyperbolic kinetics with fixed glutathione concentration and with other substrates limiting Km values for Mg2+
-
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0.0026
1,3,5-trimethyl-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]-1H-pyrazole-4-sulfonamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.1
1-(3-(1-isobutyl-1H-pyrazol-4-yl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0037
1-(3-(1H-indol-4-yl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.00035
1-(3-(3-chlorophenyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00009
1-(3-(3-fluorophenyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.003
1-(3-(3-pyridyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
1-(3-(4-chlorophenyl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0019
1-(3-(furan-2-yl)-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00015
1-(3-phenyl-1H-indazol-1-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.0003
1-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0027
1-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)butan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0065
1-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)propan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0019
1-(5-(3,5-difluorophenyl)-2H-tetrazol-2-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0015
1-(5-(3-fluorophenyl)-2H-tetrazol-2-yl)-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00012
1-(tert-butylsulfonylmethyl)-3-(3-fluorophenyl)-1H-indazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.000095
1-[3-(3-fluorophenyl)-1H-indazol-1-yl]-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH 8.0, 22°C
0.0072
1-[5-(3-chloro-4-methylphenyl)-2H-tetrazol-2-yl]-3,3-dimethylbutan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0022
2,4-dimethyl-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]-1,3-thiazole-5-sulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.024
2,4-dimethyl-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]thiophene-3-sulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0063
2,6-dichloro-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.016
2,6-dichloro-N-[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0061
2,6-difluoro-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
2,6-dimethoxy-N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0011
2-((tert-butylsulfonyl)methyl)-4-(2-fluorophenyl)thiazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0032
2-((tert-butylsulfonyl)methyl)-4-(2-methylphenyl)thiazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0004
2-((tert-butylsulfonyl)methyl)-4-(3-chlorophenyl)thiazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.012
2-((tert-butylsulfonyl)methyl)-4-(3-methoxyphenyl)thiazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0012
2-((tert-butylsulfonyl)methyl)-4-(phenyl)thiazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00014
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-1-(piperidin-1-yl)ethanone
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00011
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-N,N-dimethylacetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00086
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-N-(2-(4-methylpiperazin-1-yl)ethyl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00083
2-(3-(3-fluorophenyl)-1H-indazol-1-yl)-N-(3-(4-methylpiperazin-1-yl)propyl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0006
2-(5-(3,5-dichlorophenyl)-2H-tetrazol-2-yl)-N,N-dimethylacetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.045
2-(5-(3,5-difluorophenyl)-2H-tetrazol-2-yl)-1-phenylethanone
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00015
2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0011
2-(tert-butylsulfonylmethyl)-4-(3-trifluoromethylphenyl)thiazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.037
2-methyl-N-((5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl)propane-2-sulfonamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.00014
2-[3-(3-fluorophenyl)-1H-indazol-1-yl]-1-(piperidin-1-yl)ethanone
Trypanosoma brucei
-
pH 8.0, 22°C
0.1
3,3-dimethyl-1-(3-(1-methyl-1H-indol-4-yl)-1H-indazol-1-yl)butan-2-one
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.1
3,3-dimethyl-1-(3-(naphthalen-2-yl)-1H-indazol-1-yl)butan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0003
3,3-dimethyl-1-(3-(pyridin-4-yl)-1H-indazol-1-yl)butan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0004
3,3-dimethyl-1-(3-(thiophen-3-yl)-1H-indazol-1-yl)butan-2-one
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.0088
3,3-dimethyl-1-(5-phenyl-2H-tetrazol-2-yl)butan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0035
3,3-dimethyl-1-[5-[3-(trifluoromethyl)phenyl]-2H-tetrazol-2-yl]butan-2-one
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
3,3-dimethyl-1-[5-[4-(trifluoromethyl)phenyl]-2H-tetrazol-2-yl]butan-2-one
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.0058
3,5-dichloro-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
3-((3-(3-fluorophenyl)-1H-indazol-1-yl)methyl)-5-methylisoxazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
3-((3-(3-fluorophenyl)-1H-indazol-1-yl)methyl)-5-phenylisoxazole
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.0029
3-(3-fluorophenyl)-1-(pyridin-2-ylmethyl)-1H-indazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0045
3-(3-fluorophenyl)-1-(pyridin-3-ylmethyl)-1H-indazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00008
3-(tert-butylsulfonylmethyl)-1-(3-fluorophenyl)-1H-indazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00022
3-(tert-butylsulfonylmethyl)-1-phenyl-1H-indazole
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.028
4-((3-(3-fluorophenyl)-1H-indazol-1-yl)methyl)-2,5-dimethyloxazole
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.0087
4-acetyl-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.011
4-methoxy-N-[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.004
4-methoxy-N-[[3-(2-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
4-methyl-N-[(2-phenyl-1,3-thiazol-4-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0313
5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0117
betulin
Leishmania donovani
pH 8.0, 25°C, recombinant enzyme
0.0134
conessine
Leishmania donovani
pH 8.0, 25°C, recombinant enzyme
0.02
methyl (3-phenyl-1H-indazol-1-yl)acetate
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0138
N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0153
N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.1
N,N-dibenzyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0002
N,N-diethyl-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.015
N,N-diethyl-2-(3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0056
N-(1-phenylethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.000145
N-(2-(dimethylamino)ethyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.03
N-(2-methoxyethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.005
N-(2-phenylethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00035
N-(3-(1H-imidazol-1-yl)propyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.000045
N-(3-(dimethylamino)propyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
N-(4-chlorobenzyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.003
N-(4-chlorobenzyl)-2-(3-(trifluoromethyl)-4,5,6,7-tetrahydro-1Hindazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.05
N-(4-[methyl[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]sulfamoyl]phenyl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0059
N-(4-[[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]sulfamoyl]phenyl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.012
N-(butan-2-yl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0039
N-(thiophen-2-ylmethyl)-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
N-cyclohexyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.00017
N-methyl-(3-(dimethylamino)propyl)-2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
N-phenyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.013
N-propyl-2-[3-(trifluoromethyl)-4,5,6,7-tetrahydro-1H-indazol-1-yl]acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.002 - 4
N-[(2-phenyl-1,3-thiazol-4-yl)methyl]naphthalene-2-sulfonamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.05
N-[(3-phenyl-1,2,4-oxadiazol-5-yl)methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.1
N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]naphthalene-1-sulfonamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.0085
N-[(5-methyl-2-phenyl-2H-1,2,3-triazol-4-yl)methyl]thiophene-2-sulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.00035
N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0136
N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0321
N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0037
N-[4-([[3-(2-chlorophenyl)-1,2,4-oxadiazol-5-yl]methyl]sulfamoyl)phenyl]acetamide
Trypanosoma brucei
-
above, pH and temperature not specified in the publication
0.0017
N-[4-([[3-(2-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]sulfamoyl)phenyl]acetamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.025
N-[[3-(2-chlorophenyl)-1,2,4-oxadiazol-5-yl]methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.015
N-[[3-(2-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]benzenesulfonamide
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.0478
N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0259
N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0515
N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0234
N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0567
N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0263
N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0391
N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0496
N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine
Leishmania infantum
pH 7.4, 22°C, recombinant enzyme
0.0029
tert-butyl-4-(2-(2-(3-(3-fluorophenyl)-1H-indazol-1-yl)acetamido)ethyl)piperazine-1-carboxylate
Trypanosoma brucei
-
pH and temperature not specified in the publication
0.018
tomatine
Leishmania donovani
pH 8.0, 25°C, recombinant enzyme
0.0112
uvaol
Leishmania donovani
pH 8.0, 25°C, recombinant enzyme
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malfunction
genetic knockout mutants of both alleles are not viable, chemical targeting of LiTRYS with a drug-like compound FS-554 also leads to parasite death
malfunction
-
genetic knockout mutants of both alleles are not viable, chemical targeting of LiTRYS with a drug-like compound FS-554 also leads to parasite death
-
metabolism
-
trypanothione synthetase catalyses the two-step biosynthesis of trypanothione from spermidine and glutathione
metabolism
trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase
metabolism
trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase
metabolism
trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase
metabolism
trypanothione synthetase (TryS) of thiol metabolic pathway is the sole enzyme responsible for the biosynthesis of trypanothione in Leishmania donovani
metabolism
-
trypanothione synthetase (TryS) of thiol metabolic pathway is the sole enzyme responsible for the biosynthesis of trypanothione in Leishmania donovani
-
metabolism
-
trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase
-
metabolism
-
trypanothione synthetase (TryS) of thiol metabolic pathway is the sole enzyme responsible for the biosynthesis of trypanothione in Leishmania donovani
-
metabolism
-
trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase
-
metabolism
-
trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase
-
physiological function
-
culture of a tetracyclinedependent conditional double knockout cDKO parasites without tetracycline induction results in loss of trypanothione and accumulation of glutathione, followed by growth inhibition and cell lysis after 6 days. In the absence of inducer, cDKO cells are unable to infect mice, confirming that the enzyme is essential for virulence in vivo as well as in vitro
physiological function
in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis
physiological function
in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis
physiological function
in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis
physiological function
the enzyme is involved in Amp B resistance and during oxidative stress to help the parasites survival
physiological function
trypanothione synthetase, EC 6.3.1.9, but not glutathionylspermidine synthetase, EC 6.3.1.8, is essential for Leishmania infantum
physiological function
-
trypanothione synthetase, EC 6.3.1.9, but not glutathionylspermidine synthetase, EC 6.3.1.8, is essential for Leishmania infantum
-
physiological function
-
the enzyme is involved in Amp B resistance and during oxidative stress to help the parasites survival
-
physiological function
-
in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis
-
physiological function
-
the enzyme is involved in Amp B resistance and during oxidative stress to help the parasites survival
-
physiological function
-
in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis
-
physiological function
-
in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis
-
additional information
-
binding sites for glutathione, ATP and two Mg2+ ions, molecular docking and molecular dynamics simulations, strructure modeling, detailed overview
additional information
-
the steady-state kinetic analysis discard a ping-pong mechanism
additional information
-
docking study and structure homology modeling using Leishmania major TryS, PDB ID 2VOB, structure as template, overview
additional information
-
docking study and structure homology modeling using Leishmania major TryS, PDB ID 2VOB, structure as template, overview
-
additional information
-
the steady-state kinetic analysis discard a ping-pong mechanism
-
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Nadeau, K.C.
Biochemical studies on protein folding chaperones (HSP90 and cyclophilin) and on trypanosomal enzymes (trypanothione and glutathionylspermidine synthetases) (heat shock protein)
Diss. Abstr. Int. B
56
3744
1995
Crithidia fasciculata
-
brenda
Smith, K.; Nadeau, K.; Bradley, M.; Walsh, C.; Fairlamb, A.H.
Purification of glutathionylspermidine and trypanothione synthetase from Crithidia fasciculata
Protein Sci.
1
874-883
1992
Crithidia fasciculata
brenda
Tetaud, E.; Manai, F.; Barrett, M.P.; Nadeau, K.; Walsh, C.T.; Fairlamb, A.H.
Cloning and characterization of the two enzymes responsible for trypanothione biosynthesis in Crithidia fasciculata
J. Biol. Chem.
273
19383-19390
1998
Crithidia fasciculata (O60993), Crithidia fasciculata
brenda
Henderson, G.B.; Yamaguchi, M.; Novoa, L.; Fairlamb, A.H.; Cerami, A.
Biosynthesis of the trypanosomatid metabolite trypanothione: purification and characterization of trypanothione synthetase from Crithidia fasciculata
Biochemistry
29
3924-3929
1990
Crithidia fasciculata
brenda
Ondarza, R.N.; Hernandez, E.; Iturbe, A.; Hurtado, G.; Tamayo, E.M.
Detection by HPLC of a trypanothione synthetase activity in vitro from Entamoeba histolytica
Biotechnol. Appl. Biochem.
30
41-45
1999
Crithidia luciliae, Entamoeba histolytica
-
brenda
Oza, S.L.; Tetaud, E.; Ariyanayagam, M.R.; Warnon, S.S.; Fairlamb, A.H.
A Single Enzyme Catalyses Formation of Trypanothione from Glutathione and Spermidine in Trypanosoma cruzi
J. Biol. Chem.
277
35853-35861
2002
Trypanosoma cruzi (Q9GT49), Trypanosoma cruzi
brenda
Ariyanayagam, M.R.; Oza, S.L.; Guther, M.L.S.; Fairlamb, A.H.
Phenotypic analysis of trypanothione synthetase knockdown in the African trypanosome
Biochem. J.
391
425-432
2005
Trypanosoma brucei
brenda
Comini, M.A.; Guerrero, S.A.; Haile, S.; Menge, U.; Lunsdorf, H.; Flohe, L.
Valdiation of Trypanosoma brucei trypanothione synthetase as drug target
Free Radic. Biol. Med.
36
1289-1302
2004
Trypanosoma brucei
brenda
Comini, M.; Menge, U.; Wissing, J.; Flohe, L.
Trypanothione synthesis in Crithidia revisited
J. Biol. Chem.
280
6850-6860
2005
Crithidia fasciculata (Q5DM89), Crithidia fasciculata
brenda
Oza, S.L.; Shaw, M.P.; Wyllie, S.; Fairlamb, A.H.
Trypanothione biosynthesis in Leishmania major
Mol. Biochem. Parasitol.
139
107-116
2005
Leishmania major (Q711P7), Leishmania major
brenda
Oza, S.L.; Wyllie, S.; Fairlamb, A.H.
Mapping the functional synthetase domain of trypanothione synthetase from Leishmania major
Mol. Biochem. Parasitol.
149
117-120
2006
Leishmania major (Q711P7), Leishmania major
brenda
Fyfe, P.K.; Oza, S.L.; Fairlamb, A.H.; Hunter, W.N.
Leishmania trypanothione synthetase-amidase structure reveals a basis for regulation of conflicting synthetic and hydrolytic activities
J. Biol. Chem.
283
17672-17680
2008
Leishmania major (Q711P7)
brenda
Comini, M.A.; Dirdjaja, N.; Kaschel, M.; Krauth-Siegel, R.L.
Preparative enzymatic synthesis of trypanothione and trypanothione analogues
Int. J. Parasitol.
39
1059-1062
2009
Crithidia fasciculata
brenda
Torrie, L.S.; Wyllie, S.; Spinks, D.; Oza, S.L.; Thompson, S.; Harrison, J.R.; Gilbert, I.H.; Wyatt, P.G.; Fairlamb, A.H.; Frearson, J.A.
Chemical validation of trypanothione synthetase: a potential drug target for human trypanosomiasis
J. Biol. Chem.
284
36137-36145
2009
Trypanosoma brucei
brenda
Wyllie, S.; Oza, S.L.; Patterson, S.; Spinks, D.; Thompson, S.; Fairlamb, A.H.
Dissecting the essentiality of the bifunctional trypanothione synthetase-amidase in Trypanosoma brucei using chemical and genetic methods
Mol. Microbiol.
74
529-540
2009
Trypanosoma brucei
brenda
Fitzgerald, M.P.; Madsen, J.M.; Coleman, M.C.; Teoh, M.L.; Westphal, S.G.; Spitz, D.R.; Radi, R.; Domann, F.E.
Transgenic biosynthesis of trypanothione protects Escherichia coli from radiation-induced toxicity
Radiat. Res.
174
290-296
2010
Trypanosoma cruzi
brenda
Saudagar, P.; Dubey, V.K.
Cloning, expression, characterization and inhibition studies on trypanothione synthetase, a drug target enzyme, from Leishmania donovani
Biol. Chem.
392
1113-1122
2011
Leishmania donovani (G5D5D5), Leishmania donovani, Leishmania donovani UR6 (G5D5D5)
brenda
Spinks, D.; Torrie, L.S.; Thompson, S.; Harrison, J.R.; Frearson, J.A.; Read, K.D.; Fairlamb, A.H.; Wyatt, P.G.; Gilbert, I.H.
Design, synthesis and biological evaluation of Trypanosoma brucei trypanothione synthetase inhibitors
ChemMedChem
7
95-106
2012
Trypanosoma brucei
brenda
Leroux, A.E.; Haanstra, J.R.; Bakker, B.M.; Krauth-Siegel, R.L.
Dissecting the catalytic mechanism of Trypanosoma brucei trypanothione synthetase by kinetic analysis and computational modeling
J. Biol. Chem.
288
23751-23764
2013
Trypanosoma brucei, Trypanosoma brucei 449
brenda
Koch, O.; Cappel, D.; Nocker, M.; Jaeger, T.; Flohe, L.; Sotriffer, C.A.; Selzer, P.M.
Molecular dynamics reveal binding mode of glutathionylspermidine by trypanothione synthetase
PLoS ONE
8
e56788
2013
Leishmania major
brenda
Vazquez, C.; Mejia-Tlachi, M.; Gonzalez-Chavez, Z.; Silva, A.; Rodriguez-Zavala, J.S.; Moreno-Sanchez, R.; Saavedra, E.
Buthionine sulfoximine is a multitarget inhibitor of trypanothione synthesis in Trypanosoma cruzi
FEBS Lett.
591
3881-3894
2017
Trypanosoma cruzi, Trypanosoma cruzi DTU I
brenda
Sousa, A.F.; Gomes-Alves, A.G.; Benitez, D.; Comini, M.A.; Flohe, L.; Jaeger, T.; Passos, J.; Stuhlmann, F.; Tomas, A.M.; Castro, H.
Genetic and chemical analyses reveal that trypanothione synthetase but not glutathionylspermidine synthetase is essential for Leishmania infantum
Free Radic. Biol. Med.
73
229-238
2014
Leishmania infantum (A4I2Z3), Leishmania infantum, Leishmania infantum MHOM / MA / 67 / ITMAP263 (A4I2Z3)
brenda
Benitez, D.; Medeiros, A.; Fiestas, L.; Panozzo-Zenere, E.A.; Maiwald, F.; Prousis, K.C.; Roussaki, M.; Calogeropoulou, T.; Detsi, A.; Jaeger, T.; Sarlauskas, J.; Peterlin Masic, L.; Kunick, C.; Labadie, G.R.; Flohe, L.; Comini, M.A.
Identification of novel chemical scaffolds inhibiting trypanothione synthetase from pathogenic trypanosomatids
PLoS Negl. Trop. Dis.
10
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2016
Leishmania infantum (A4I2Z3), Leishmania infantum, Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263 (A4I2Z3), Trypanosoma brucei brucei (Q8IEX1), Trypanosoma brucei brucei 427 (Q8IEX1), Trypanosoma cruzi (Q9GT49), Trypanosoma cruzi, Trypanosoma cruzi CL Brener (Q9GT49)
brenda
Equbal, A.; Suman, S.S.; Anwar, S.; Singh, K.P.; Zaidi, A.; Sardar, A.H.; Das, P.; Ali, V.
Stage-dependent expression and up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani
PLoS ONE
9
e97600
2014
Leishmania donovani (Q8IFU8), Leishmania donovani, Leishmania donovani MHOM / IN / 80 / Dd8 (Q8IFU8), Leishmania donovani MHOM / IN / 83 / Ag (Q8IFU8)
brenda