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evolution
MpLCYb belongs to the LCYb1 (chloroplast-specific) family rather than to the LCYb2 (chromoplast-specific) family, hylogenetic tree
malfunction
the cruA::aadA mutant does not produce carotenoids with cyclic end groups. The cruA mutant shows a severe growth defect. Wild-type Synechococcus sp. PCC 7002 produces large amounts of the dicyclic carotenoids beta-carotene and zeaxanthin as well as the monocyclic myxoxanthophyll but does not accumulate lycopene or gamma-carotene. The cruA mutant strain accumulates significant amounts of lycopene and gamma-carotene
malfunction
transgenic plants with higher or reduced levels of DcLcyb1 have incremented or reduced levels of chlorophyll, total carotenoids and beta-carotene in leaves and in the storage roots, respectively. In addition, changes in the expression of DcLcyb1 are accompanied by a modulation in the expression of key endogenous carotenogenic genes
malfunction
down-regulation of sweetpotato lycopene beta-cyclase gene enhances tolerance to abiotic stress in transgenic calli
malfunction
-
the suppression of enzyme expression leads to lower expression levels of genes in the carotenoid biosynthesis pathway and to reduced accumulation of carotenoids, chlorophyll, and abscisic acid
metabolism
Citrus sp.
-
key enzyme for the synthesis of beta-carotene
metabolism
-
the enzyme is downregulated before fruit ripening through a tightly regulated physiological mechanism
metabolism
the enzyme is involved in the pathway of carotenoid biosynthesis, overview
metabolism
-
the cyclization enzyme is involved in carotenoid accumulation
metabolism
the enzyme is involved in the astaxanthin biosynthesis pathway
metabolism
-
the enzyme is involved in the astaxanthin biosynthesis pathway
-
physiological function
lycopene beta-cyclase is a key enzyme involved directly in the synthesis of alpha-carotene and beta-carotene through the cyclization of lycopene. The enzyme does not possess an organ specific function and modulates carotenoid gene expression and accumulation in carrot leaves and storage roots. DcLcyb1 participates in the regulation of endogenous DcPsy1, DcPsy2 and DcLcyb2 carotenogenic genes
physiological function
Citrus sp.
-
regulatory effect of Lycb-1 gene on plant carotenoid metabolism and fruit transcriptome, overview
physiological function
-
the enzyme confers salt and drought stress tolerance in Nicotiana tabacum
physiological function
the enzyme expression produces an increment in carotenoid and chlorophyll levels through the up-regulation of endogenous genes. Tobacco plants with higher recombinant enzyme expression levels show an increase in biomass and fitness
physiological function
-
the lycopene beta-cyclase gene LCYB2 enhances carotenoid contents and abiotic stress tolerance through positive regulation of carotenoid and abscisic acid biosynthesis pathways in transgenic sweetpotato
physiological function
the salt tolerance of the enzyme is ascribed to the enhanced carotenoid content for its reactive oxygen species scavenging ability, photoprotection and membrane stabilization
physiological function
-
transgenic tomato lines show increased levels of beta-carotene in ripe fruit and tolerance to 2-(4-chlorophenylthio)triethylamine
additional information
-
beta-carotene is the most abundant carotenoid in the anthers of the species reaching 93% of the total carotenoids in LA2405
additional information
-
beta-carotene is the most abundant carotenoid in the anthers of the species reaching 95% of the total carotenoids in P86
additional information
DcLCYB1 has conserved motifs related to lycopene beta-cyclases such as a cyclase motif I and II, a LCY specific motif, a conserved region beta-LCY, a di-nucleotide binding site, a charged region and the beta-LCY motif, beta-LCY CAD region, domains described as essential for lycopene beta-cyclase activity
additional information
-
DcLCYB1 has conserved motifs related to lycopene beta-cyclases such as a cyclase motif I and II, a LCY specific motif, a conserved region beta-LCY, a di-nucleotide binding site, a charged region and the beta-LCY motif, beta-LCY CAD region, domains described as essential for lycopene beta-cyclase activity
additional information
-
beta-carotene is the most abundant carotenoid in the anthers of the species reaching 95% of the total carotenoids in P86
-
additional information
-
beta-carotene is the most abundant carotenoid in the anthers of the species reaching 93% of the total carotenoids in LA2405
-
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a lycopene beta-cyclase/lycopene epsilon-cyclase/light-harvesting complex-fusion protein is expressed in Escherichia coli BL21(DE3) cells
-
astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution
expressed in Arabidopsis thaliana protoplasts
expressed in Escherichia coli
expressed in Escherichia coli TOPO10 cells
expressed in fruits of Solanum lycopersicum cultivar Ailsa Craig
-
expressed in fruits of Solanum lycopersicum cultivar Arka Ahuti
-
expressed in Ipomoea batatas sweetpotato cultivar. Shangshu 19
-
expressed in Nicotiana tabacum
expressed in Nicotiana tabacum cultivar Xanthi NN
expression in Escherichia coli
expression in Escherichia coli results in the simultaneous accumulation of alpha- beta-, delta-, and epsilon-carotene
-
gene CmLcyb1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, real time quantitative RT-PCR expression analysis
-
gene crtY, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus, 25°C is the optimum growth temperature for obtaining soluble lycopene cyclase in an active form
gene DcLcyb1, quantitative RT-PCR expression analysis, overexpression and heterologous complementation in enzyme-deficient Escherichia coli, transgenic expression of GFP-tagged enzyme in Nicotiana tabacum plants under control of ad35SCaMV promoter
gene Lcy-b, expression in tomato fruite under control of the 35S CaMV promoter in cotyledons via Agrobacterium tumefaciens-mediated transformation, the transgenic plants show increased lutein levels, quantitative real-time quantitative PCR expression analysis
-
gene lcy-B, real-time quantitative RT-PCR expression analysis
gene Lyc-beta, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression in Escherichia coli strain BL21(DE3) leading to pigmentation of the transgenic bacteria, strongest at 28°C in the dark
gene Lycb-1, constitutive heterologous expression in Solanum lycopersicum fruits. In the transgenic line, the downstream alpha-branch metabolic fluxes are repressed during the three developmental stages while alpha-carotene content increases in the ripe stage, the beta-carotene level of transformant increases 4.1fold, and the total carotenoid content increases by 30% in the fruits. The constitutive expression of Lycb-1 affects a number of pathways including the synthesis of fatty acids, flavonoids and phenylpropanoids, the degradation of limonene and pinene, starch and sucrose metabolism and photosynthesis. Carotenoids contents in transgenic fruits at different stages of ripening, overview
Citrus sp.
-
gene MpLCYb, DNA and amino acid sequence determination and analysis
overexpression in Saccharomyces cerevisiae. Yeast cells expressing CrtI, CrtYB, and CrtS from Xanthophyllomyces dendrorhous accumulate beta-carotene but not astaxanthin
recombinant expression of the gene in lycopene-producing Escherichia coli results in the accumulation of b-carotene in the cells
screening and cloning of gene crtYB, encoding lycopene cyclase and phytoene synthase, DNA and amino acid sequence determination and analysis, sequence comparisons
expressed in Escherichia coli
-
expressed in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
gene lcy-B, real-time quantitative RT-PCR expression analysis
-
gene lcy-B, real-time quantitative RT-PCR expression analysis
-
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2003
Saccharolobus solfataricus (Q97UT9), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97UT9)
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Cucumis melo
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23
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Paracoccus haeundaensis (Q24K62), Paracoccus haeundaensis
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Solanum lycopersicum
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Marchantia polymorpha (W0S9F1)
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PLoS ONE
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Citrus sp.
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Daucus carota (Q2VEX7), Daucus carota
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Phaffia rhodozyma (Q7Z859), Phaffia rhodozyma CGMCC As2.1557 (Q7Z859)
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Phaffia rhodozyma (Q7Z859), Phaffia rhodozyma ATCC 24202 (Q7Z859)
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Citrullus lanatus
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Nicotiana tabacum
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