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2,3-dimethyl-cis,cis-muconate
2,3-dimethylmuconolactone
-
-
-
?
2,4-dichloro-cis,cis-muconate
?
2,4-dimethyl-cis,cis-muconate
2,4-dimethylmuconolactone
-
-
-
?
2,5-dihydro-5-oxofuran-2-acetate
cis,cis-hexadienedioate
-
-
-
-
?
2-chloro-cis,cis-muconate
(+)-2-chloromuconolactone + (+)-5-chloromuconolactone
-
-
mutant enzymes I54V and I54V/Y59W form 5-chloromuconolactone almost exclusively. Mutant enzymes F329I and Y59W form 2-chloromuconolactone as the main product. Mutant enzyme A271S hehaves similar to wild-type enzyme
?
2-chloro-cis,cis-muconate
5-chloromuconolactone + 2-chloromuconolactone
2-Chloro-cis,cis-muconate
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
2-Methyl-cis,cis-muconate
?
3-Bromo-cis,cis-muconate
?
-
at 9% of the activity relative to 3-methyl-cis,cis-muconate
-
-
?
3-chloro-cis,cis-muconate
4-chloro-muconolactone
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
3-chloro-cis,cis-muconate
?
-
-
?
3-chloro-cis,cis-muconate
cis-dienelactone + protoanemonin + Cl- + H2O + CO2
3-chloro-cis,cis-muconate
protoanemonin + ?
-
-
formed by wild-type enzyme and mutant enzymes I54V, A271S, K276N, F329I, L333V, I54V/F329I
?
3-chloromuconate
cis-dienelactone + protoanemonin
the highest turnover rate, 10fold higher than with 3-methylmuconate, is observed with 3-chloromuconate
-
-
?
3-ethyl-cis,cis-muconate
4-ethylmuconolactone
3-Fluoro-cis,cis-muconate
(+)-4-Fluoromuconolactone
3-Fluoro-cis,cis-muconate
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
3-Methyl-cis,cis-muconate
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
cis,cis-hexadienedioate
2,5-dihydro-5-oxofuran-2-acetate
cis,cis-muconate
(+)-(4S)-muconolactone
-
syn-cycloisomerization
-
-
?
cis,cis-Muconate
(+)-Muconolactone
cis,cis-muconate
(4S)-muconolactone
cis,cis-muconate
muconolactone
3-chloro-cis,cis-muconate
additional information
-
2,4-dichloro-cis,cis-muconate
?
-
-
-
?
2,4-dichloro-cis,cis-muconate
?
-
-
-
?
2,4-dichloro-cis,cis-muconate
?
-
-
?
2,4-dichloro-cis,cis-muconate
?
-
-
-
?
2-chloro-cis,cis-muconate
5-chloromuconolactone + 2-chloromuconolactone
-
-
?
2-chloro-cis,cis-muconate
5-chloromuconolactone + 2-chloromuconolactone
-
-
-
?
2-chloro-cis,cis-muconate
5-chloromuconolactone + 2-chloromuconolactone
-
-
-
?
2-chloro-cis,cis-muconate
5-chloromuconolactone + 2-chloromuconolactone
-
-
-
-
?
2-chloro-cis,cis-muconate
5-chloromuconolactone + 2-chloromuconolactone
-
-
-
-
?
2-Chloro-cis,cis-muconate
?
-
-
-
?
2-Chloro-cis,cis-muconate
?
-
0.8% of the activity relative to cis,cis-muconate
-
-
?
2-Chloro-cis,cis-muconate
?
-
0.8% of the activity relative to cis,cis-muconate
-
-
?
2-Chloro-cis,cis-muconate
?
-
-
-
-
?
2-Chloro-cis,cis-muconate
?
-
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
?
2-methyl-cis,cis-muconate
2-methylmuconolactone
-
-
-
?
2-Methyl-cis,cis-muconate
?
-
-
-
?
2-Methyl-cis,cis-muconate
?
-
at 80% of the activity relative to cis,cis-muconate
-
-
?
2-Methyl-cis,cis-muconate
?
-
at 80% of the activity relative to cis,cis-muconate
-
-
?
2-Methyl-cis,cis-muconate
?
-
-
?
2-Methyl-cis,cis-muconate
?
-
90% of the activity relative to cis,cis-muconate
-
-
?
2-Methyl-cis,cis-muconate
?
-
90% of the activity relative to cis,cis-muconate
-
-
?
2-Methyl-cis,cis-muconate
?
-
-
-
-
?
2-Methyl-cis,cis-muconate
?
-
at 6.1% of the activity relative to cis,cis-muconate
-
-
?
2-Methyl-cis,cis-muconate
?
-
-
-
-
?
2-Methyl-cis,cis-muconate
?
-
at 6.1% of the activity relative to cis,cis-muconate
-
-
?
2-methylmuconate
?
-
-
-
?
2-methylmuconate
?
-
27% activity compared to cis,cis-muconate
-
-
?
2-methylmuconate
?
-
27% activity compared to cis,cis-muconate
-
-
?
3-chloro-cis,cis-muconate
4-chloro-muconolactone
-
-
unstable
-
?
3-chloro-cis,cis-muconate
4-chloro-muconolactone
-
-
unstable
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
at 11% of the activity relative to 3-methyl-cis,cis-muconate
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
7.1% of the activity relative to cis,cis-muconate
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
7.1% of the activity relative to cis,cis-muconate
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
-
-
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
dehalogenation is not catalyzed
?
3-Chloro-cis,cis-muconate
5-Chloromuconolactone
-
-
dehalogenation is not catalyzed
?
3-chloro-cis,cis-muconate
cis-dienelactone + protoanemonin + Cl- + H2O + CO2
-
in vitro activity
product ratio is 76:24, protoanemonin is antibiotically active
-
?
3-chloro-cis,cis-muconate
cis-dienelactone + protoanemonin + Cl- + H2O + CO2
-
in vitro activity
product ratio is 76:24, protoanemonin is antibiotically active
-
?
3-chloromuconate
?
-
9% activity compared to cis,cis-muconate
-
-
?
3-chloromuconate
?
-
9% activity compared to cis,cis-muconate
-
-
?
3-ethyl-cis,cis-muconate
4-ethylmuconolactone
-
-
-
-
?
3-ethyl-cis,cis-muconate
4-ethylmuconolactone
-
-
-
-
?
3-ethyl-cis,cis-muconate
4-ethylmuconolactone
-
-
-
?
3-ethyl-cis,cis-muconate
4-ethylmuconolactone
-
-
-
?
3-Fluoro-cis,cis-muconate
(+)-4-Fluoromuconolactone
-
-
-
?
3-Fluoro-cis,cis-muconate
(+)-4-Fluoromuconolactone
-
-
-
?
3-Fluoro-cis,cis-muconate
(+)-4-Fluoromuconolactone
-
-
-
?
3-Fluoro-cis,cis-muconate
(+)-4-Fluoromuconolactone
-
-
-
?
3-Fluoro-cis,cis-muconate
(+)-4-Fluoromuconolactone
-
at 17% of the activity relative to 3-methyl-cis,cis-muconate
-
-
?
3-Fluoro-cis,cis-muconate
?
-
-
-
?
3-Fluoro-cis,cis-muconate
?
-
-
-
?
3-Fluoro-cis,cis-muconate
?
-
-
-
?
3-Fluoro-cis,cis-muconate
?
-
-
?
3-Fluoro-cis,cis-muconate
?
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
?
3-methyl-cis,cis-muconate
4-methylmuconolactone
-
-
-
?
3-Methyl-cis,cis-muconate
?
-
-
-
?
3-Methyl-cis,cis-muconate
?
-
enzyme of the beta-ketoadipate pathway that also functions in the reaction sequence for converting 3-methyl-cis,cis-muconate
-
-
?
3-Methyl-cis,cis-muconate
?
-
-
-
?
3-Methyl-cis,cis-muconate
?
-
-
?
3-Methyl-cis,cis-muconate
?
-
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
-
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
11.7% of the activity relative to cis,cis-muconate
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
11.7% of the activity relative to cis,cis-muconate
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
-
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
at 5.6% of the activity relative to cis,cis-muconate
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
-
-
-
?
3-Methyl-cis,cis-muconate
beta-Methylmuconolactone
-
at 5.6% of the activity relative to cis,cis-muconate
-
-
?
3-methylmuconate
?
-
-
-
?
3-methylmuconate
?
-
26% activity compared to cis,cis-muconate
-
-
?
3-methylmuconate
?
-
26% activity compared to cis,cis-muconate
-
-
?
cis,cis-hexadienedioate
2,5-dihydro-5-oxofuran-2-acetate
-
conversion of cis,cis-muconate to muconolactone, two proximal Lys residues exist in the active sites, which can potentially lead, via a single intermediate form, to the formation of either the syn- or anti-products. The enzyme from Mycobacterium smegmatis forms a syn-product, overview
-
-
r
cis,cis-hexadienedioate
2,5-dihydro-5-oxofuran-2-acetate
-
conversion of cis,cis-muconate to muconolactone, two proximal Lys residues exist in the active sites, which can potentially lead, via a single intermediate form, to the formation of either the syn- or anti-products. The enzyme from Pseudomonas fluorescens forms an anti-product, overview
-
-
r
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
i.e. 5-oxo-2,5-dihydrofuran-2-acetate, , (4S)-muconolactone
?
cis,cis-Muconate
(+)-Muconolactone
-
no activity against trans-trans-muconate
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
2970, 2972, 3636, 3639, 3643, 3645, 3649, 3650, 3651, 3652, 3653, 390694 -
-
?
cis,cis-Muconate
(+)-Muconolactone
-
i.e. cis,cis-2,4-hexadiendioate, , r
-
?
cis,cis-Muconate
(+)-Muconolactone
-
i.e. cis,cis-2,4-hexadiendioate, , r
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-Muconate
(+)-Muconolactone
-
-
-
-
?
cis,cis-muconate
(4S)-muconolactone
syn-cycloisomerization
-
-
?
cis,cis-muconate
(4S)-muconolactone
-
-
-
-
?
cis,cis-muconate
(4S)-muconolactone
-
-
-
?
cis,cis-muconate
(4S)-muconolactone
-
-
-
?
cis,cis-Muconate
?
-
inducible by benzoate
-
-
?
cis,cis-Muconate
?
-
inducible enzyme
-
-
?
cis,cis-Muconate
?
-
enzyme of the beta-ketoadipate pathway
-
-
?
cis,cis-Muconate
?
-
the enzyme plays a key role in the beta-ketoadipate pathway of benzoate degradation
-
-
?
cis,cis-Muconate
?
-
enzyme of the beta-ketoadipate pathway
-
-
?
cis,cis-Muconate
?
-
enzyme of the benzoate catabolism
-
-
?
cis,cis-Muconate
?
-
enzyme of the beta-ketoadipate pathway
-
-
?
cis,cis-Muconate
?
-
enzyme of the benzoate catabolism
-
-
?
cis,cis-Muconate
?
-
catalyzes the second step of the beta-ketoadipate pathway
-
-
?
cis,cis-Muconate
?
-
catalyzes the second step of the beta-ketoadipate pathway
-
-
?
cis,cis-muconate
muconolactone
-
-
-
?
cis,cis-muconate
muconolactone
-
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
-
?
cis,cis-muconate
muconolactone
-
-
-
?
cis,cis-muconate
muconolactone
-
different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18
-
?
cis,cis-muconate
muconolactone
-
-
-
?
cis,cis-muconate
muconolactone
-
different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18
-
?
cis,cis-muconate
muconolactone
-
-
-
-
?
cis,cis-muconate
muconolactone
-
-
-
-
?
cis,cis-muconate
muconolactone
-
-
-
-
?
cis,cis-muconate
muconolactone
-
i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity
i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate
-
?
cis,cis-muconate
muconolactone
-
-
-
-
?
cis,cis-muconate
muconolactone
-
i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity
i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate
-
?
cis,cis-muconate
muconolactone
-
-
-
-
?
cis,cis-muconate
muconolactone
-
-
-
?
cis,cis-muconate
muconolactone
-
-
-
?
cis,cis-muconate
muconolactone
-
-
?
cis,cis-muconate
muconolactone
-
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
-
?
cis,cis-muconate
muconolactone
-
-
-
?
cis,cis-muconate
muconolactone
-
the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone
-
?
cis,cis-muconate
muconolactone
-
-
-
?
cis,cis-muconate
muconolactone
-
enzyme is involved in beta-ketoadipate pathway
-
?
cis,cis-muconate
muconolactone
-
best substrate
-
-
?
cis,cis-muconate
muconolactone
-
best substrate
-
-
?
cis,trans-Muconate
?
-
no activity
-
-
?
cis,trans-Muconate
?
-
-
-
-
?
cis,trans-Muconate
?
-
-
-
-
?
3-chloro-cis,cis-muconate
additional information
-
-
-
wild-type enzyme forms protoanemonin and 2-chloroprotoanemonin, mutant enzyme K169A forms cis-dienelactone and 2-chloro-cis-dienelactone
?
additional information
?
-
-
no activity with 3-chloro-cis,cis-muconate and 2,4-dichloro-cis,cis-muconate
-
?
additional information
?
-
-
no substrate: 2-chloro-cis,cis-muconate, 2-fluoro-cis,cis-muconate
-
-
?
additional information
?
-
-
no substrate: 2-chloro-cis,cis-muconate, 2-fluoro-cis,cis-muconate
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview
-
-
?
additional information
?
-
-
the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview
-
-
?
additional information
?
-
-
no activity against 2-carboxy-cis,cis-muconate
-
-
?
additional information
?
-
-
the enzyme is a component of the beta-ketoadipate pathway
-
?
additional information
?
-
-
no activity against 2-carboxy-cis,cis-muconate
-
-
?
additional information
?
-
activity of the enzyme with muconate is negligible and at a substrate concentration of 0.1 mM substrate, the activity is only 0.4% of that with 3-chloromuconate, the enzyme is practically inactive with 2-chloromuconate
-
-
?
additional information
?
-
-
the methyl substitution at the C-2 position of muconate directs the cycloisomerization by 1,4-addition rather than 3,6-addition. The steric influence caused by methyl substitution may encourage the 1,4-cycloisomerization
-
?
additional information
?
-
-
the methyl substitution at the C-2 position of muconate directs the cycloisomerization by 1,4-addition rather than 3,6-addition. The steric influence caused by methyl substitution may encourage the 1,4-cycloisomerization
-
?
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0.026 - 0.093
2,4-dichloro-cis,cis-muconate
0.017 - 0.415
2-chloro-cis,cis-muconate
0.029 - 0.49
2-methyl-cis,cis-muconate
0.045 - 1.24
3-chloro-cis,cis-muconate
0.67
3-ethyl-cis,cis-muconate
-
24°C, pH 8.0
0.063 - 0.23
3-fluoro-cis,cis-muconate
0.006 - 0.099
3-methyl-cis,cis-muconate
0.0106
3-methylmuconate
-
0.0071 - 1.73
cis,cis-muconate
additional information
additional information
-
-
0.026
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
0.039
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
0.04
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
0.044
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
0.045
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
0.074
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
0.078
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
0.093
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
0.017
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
0.033
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
0.034
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
0.036
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
0.037
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
0.047
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
0.0559
2-chloro-cis,cis-muconate
-
-
0.059
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
0.06
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
0.29
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C
0.415
2-chloro-cis,cis-muconate
-
-
0.029
2-methyl-cis,cis-muconate
-
24°C, pH 8.0
0.0306
2-methyl-cis,cis-muconate
-
isoform CatB, 25°C
0.04
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
0.057
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
0.058
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
0.059
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
0.0667
2-methyl-cis,cis-muconate
-
-
0.068
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
0.069
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
0.105
2-methyl-cis,cis-muconate
-
-
0.11
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
0.18
2-methyl-cis,cis-muconate
-
-
0.18
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
0.49
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C
0.045
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
0.046
3-chloro-cis,cis-muconate
-
24°C, pH 8.0
0.076
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
0.099
3-chloro-cis,cis-muconate
-
isoform CatB, 25°C
0.134
3-chloro-cis,cis-muconate
-
-
0.18
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
0.21
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
0.32
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
0.41
3-chloro-cis,cis-muconate
isoform SalC, 25°C
0.55
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
0.69
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
1.24
3-chloro-cis,cis-muconate
-
-
0.063
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
0.097
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
0.1
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C
0.1
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
0.15
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
0.15
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
0.23
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
0.006
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
0.0064
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
0.0072
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
0.0091
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
0.0097
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
0.011
3-methyl-cis,cis-muconate
-
isoform CatB, 25°C
0.012
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
0.015
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
0.016
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
0.018
3-methyl-cis,cis-muconate
-
-
0.026
3-methyl-cis,cis-muconate
isoform SalC, 25°C
0.0487
3-methyl-cis,cis-muconate
-
-
0.075
3-methyl-cis,cis-muconate
-
24°C, pH 8.0
0.099
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C
0.0071
cis,cis-muconate
-
at 25°C
0.025
cis,cis-muconate
-
-
0.031
cis,cis-muconate
-
isoform CatB, 25°C
0.035
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
0.04
cis,cis-muconate
-
wild-type enzyme
0.04
cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
0.047
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W and F329I
0.052
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
0.0558
cis,cis-muconate
-
-
0.057
cis,cis-muconate
-
-
0.074
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
0.0813
cis,cis-muconate
-
-
0.09 - 0.105
cis,cis-muconate
-
-
0.096
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
0.13
cis,cis-muconate
-
pH 7.5, 25°C
0.14
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
0.143
cis,cis-muconate
-
mutant enzyme T52G/T103S
0.15
cis,cis-muconate
-
24°C, pH 8.0
0.157
cis,cis-muconate
-
mutant enzyme T52G
0.16
cis,cis-muconate
-
pH 8.0
1.73
cis,cis-muconate
isoform SalC, 25°C
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0.4 - 72000
2,4-dichloro-cis,cis-muconate
3.6 - 2940
2-chloro-cis,cis-muconate
3 - 600000
2-methyl-cis,cis-muconate
8.8 - 57600
3-chloro-cis,cis-muconate
156000 - 534000
3-fluoro-cis,cis-muconate
2 - 108000
3-methyl-cis,cis-muconate
4 - 756000
cis,cis-muconate
0.4
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
3.4
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
36.6
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
52.8
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
174
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
198
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
432
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
1320
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
1920
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
72000
2,4-dichloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
3.6
2-chloro-cis,cis-muconate
-
-
43.8
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
192
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
600
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
900
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W and A271S
1020
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C
2100
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
2880
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
2940
2-chloro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
3 - 6
2-methyl-cis,cis-muconate
-
isoform CatB, 25°C
19200
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
39000
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
84000
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
202000
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C
222000
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
234000
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
304000
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
600000
2-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N or F329I
8.8
3-chloro-cis,cis-muconate
-
-
14.4
3-chloro-cis,cis-muconate
-
isoform CatB, 25°C
120
3-chloro-cis,cis-muconate
isoform SalC, 25°C
3120
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
3840
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
6600
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
12000
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
22800
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
27600
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
57600
3-chloro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
156000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C
192000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
246000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
294000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
312000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
360000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
480000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
534000
3-fluoro-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
2
3-methyl-cis,cis-muconate
-
-
3.2
3-methyl-cis,cis-muconate
-
-
27.8
3-methyl-cis,cis-muconate
-
isoform CatB, 25°C
214
3-methyl-cis,cis-muconate
isoform SalC, 25°C
3240
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
5700
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
8400
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
16200
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
18600
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S or I54V/F329
35400
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
40800
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
44400
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
108000
3-methyl-cis,cis-muconate
-
pH 7.5, 25°C
4
cis,cis-muconate
-
mutant enzyme T52G
13.9
cis,cis-muconate
-
expressed per catalytic center
17.5
cis,cis-muconate
-
mutant enzyme T52G/F103S
24.7
cis,cis-muconate
-
-
125
cis,cis-muconate
isoform SalC, 25°C
186
cis,cis-muconate
-
isoform CatB, 25°C
186
cis,cis-muconate
isoform CatB, 25°C
210
cis,cis-muconate
-
wild-type enzyme
4080
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme L333V
32400
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V/F329I
66000
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme Y59W
126000
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme K276N
150000
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme I54V
156000
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme F329I
222000
cis,cis-muconate
-
pH 7.5, 25°C
426000
cis,cis-muconate
-
pH 7.5, 25°C, mutant enzyme A271S
756000
cis,cis-muconate
-
pH 7.5, 25°C, wild-type enzyme
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A271S
-
ratio of turnover number to Km-value: 1.5fold decrease with cis,cis-muconate as substrate, 3.3fold decrease with 2-chloro-cis,cis-muconate as substrate, 27fold increase with 3-chloro-cis,cis-muconate as substrate, 1.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.5fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.1fold increase with 2-methyl-cis,cis-muconate as substrate, 1.1fold decrease with 3-methyl-cis,cis-muconate as substrate
I54V/F329I
-
ratio of turnover number to Km-value: 81.5fold decrease with cis,cis-muconate as substrate, 2.6fold decrease with 2-chloro-cis,cis-muconate as substrate, 2.7fold increase with 3-chloro-cis,cis-muconate as substrate, 3.8fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 2fold decrease with 3-fluoro-cis,cis-muconate as substrate, 4fold decrease with 2-methyl-cis,cis-muconate as substrate, 2fold decrease with 3-methyl-cis,cis-muconate as substrate
K169A
-
70000fold reduction in activity with cis,cis-muconate, 110000fold reduction in activity with 3-fluoro-cis,cis-muconate, 310fold reduction in activity with 3-chloro-cis,cis-muconate, 2.6fold reduction in activity with 2,4-dichloro-cis,cis-muconate. Wild-type enzyme forms protoanemonin and 2-chloroprotoanemonin from 3-chloro-cis,cis-muconate, mutant enzyme K169A forms cis-dienelactone and 2-chloro-cis-dienelactone
K276N
-
ratio of turnover number to Km-value: 10.7fold decrease with cis,cis-muconate as substrate, 8.7fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.5fold increase with 3-chloro-cis,cis-muconate as substrate, 8.7fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 4.1fold decrease with 3-fluoro-cis,cis-muconate as substrate, 16.7fold decrease with 2-methyl-cis,cis-muconate as substrate, 12.1fold decrease with 3-methyl-cis,cis-muconate as substrate
L333V
-
ratio of turnover number to Km-value: 238fold decrease with cis,cis-muconate as substrate, 41.5fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 5.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.4fold decrease with 3-fluoro-cis,cis-muconate as substrate, 34.6fold decrease with 2-methyl-cis,cis-muconate as substrate, 16.5fold decrease with 3-methyl-cis,cis-muconate as substrate
Y59W
-
ratio of turnover number to Km-value: 12.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate as substrate, 4.4fold decrease with 3-fluoro-cis,cis-muconate, 1.8fold decrease with 2-methyl-cis,cis-muconate as substrate, 10.2fold decrease with 3-methyl-cis,cis-muconate as substrate
A271S
-
ratio of turnover number to Km-value: 1.5fold decrease with cis,cis-muconate as substrate, 3.3fold decrease with 2-chloro-cis,cis-muconate as substrate, 27fold increase with 3-chloro-cis,cis-muconate as substrate, 1.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.5fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.1fold increase with 2-methyl-cis,cis-muconate as substrate, 1.1fold decrease with 3-methyl-cis,cis-muconate as substrate
-
F329I
-
ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.6fold increase with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 3.2fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 1.7fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.5fold increase with 2-methyl-cis,cis-muconate as substrate, 5.8fold decrease with 3-methyl-cis,cis-muconate as substrate
-
I54V
-
ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate and 3-methyl-cis,cis-muconate as substrate, 22fold increase with 3-chloro-cis,cis-muconate as substrate, 1.67fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 5.8fold decrease with 3-fluoro-cis,cis-muconate as substrate, 8.8fold decrease with 2-methyl-cis,cis-muconate as substrate
-
K276N
-
ratio of turnover number to Km-value: 10.7fold decrease with cis,cis-muconate as substrate, 8.7fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.5fold increase with 3-chloro-cis,cis-muconate as substrate, 8.7fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 4.1fold decrease with 3-fluoro-cis,cis-muconate as substrate, 16.7fold decrease with 2-methyl-cis,cis-muconate as substrate, 12.1fold decrease with 3-methyl-cis,cis-muconate as substrate
-
Y59W
-
ratio of turnover number to Km-value: 12.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate as substrate, 4.4fold decrease with 3-fluoro-cis,cis-muconate, 1.8fold decrease with 2-methyl-cis,cis-muconate as substrate, 10.2fold decrease with 3-methyl-cis,cis-muconate as substrate
-
T52G
-
turnover number with cis,cis-muconate is 52.5fold lower than that of the wild-type enzyme, Km-value for cis,cis-muconate is 3.9fold higher than that of wild-type enzyme
T52G/F103S
-
turnover number with cis,cis-muconate is 12fold lower than that of the wild-type enzyme, Km-value for cis,cis-muconate is 3.6fold higher than that of wild-type enzyme
F329I
-
ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.6fold increase with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 3.2fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 1.7fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.5fold increase with 2-methyl-cis,cis-muconate as substrate, 5.8fold decrease with 3-methyl-cis,cis-muconate as substrate
F329I
the ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.5fold increase with 2-chloro-cis,cis-muconate, 3.4fold increased with 3-chloro-cis,cis-muconate, 3.16fold increase with 2,4-dichloro-cis,cis-muconate, 1.7fold decrease with 3-fluoro-cis,cis-muconate, 1.55fold increase with 2-methyl-cis,cis-muconate and 5.8fold increase with 3-methyl-cis,cis-muconate
I54V
-
ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate and 3-methyl-cis,cis-muconate as substrate, 22fold increase with 3-chloro-cis,cis-muconate as substrate, 1.67fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 5.8fold decrease with 3-fluoro-cis,cis-muconate as substrate, 8.8fold decrease with 2-methyl-cis,cis-muconate as substrate
I54V
ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical with 2-chloro-cis,cis-muconate, 22fold increase with 3-chloro-cis,cis-muconate, 1.6fold decrease with 2,4-dichloro-cis,cis-muconate, 5.75fold decrease with 3-fluoro-cis,cis-muconate, 6fold decrease with 2-methyl-cis,cis-muconate and 1.3fold increase with 3-methyl-cis,cis-muconate
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