in the rluC- strain, susceptibility to some antibiotics increases dramatically. The most pronounced effects in comparison with the rluC+ control are observed with clindamycin (16-fold), linezolid (8-fold), and tiamulin (4-fold)
deletion of this gene and thus the absence of these three pseudouridine residues has no detectable effect on the exponential growth rate in either rich or minimal glucose medium at temperatures ranging from 24°C to 42°C
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a proteolytically derived fragment of the enzyme consisting of residues 89-319 retains catalytic activity. Crystals of this fragment, grown by precipitation with sodium acetate at pH 8.0, belong to space group P321, with unit-cell dimensions a = b = 97.1, c = 86.3 A and have two molecules in the crystallographic asymmetric unit. Crystals diffract X-rays to at least 2.3 A resolution and appear suitable for crystal structure determination