Information on EC 5.4.2.1 - phosphoglycerate mutase

Word Map on EC 5.4.2.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
5.4.2.1
transferred to EC 5.4.2.11, EC 5.4.2.12. Now recognized as two separate enzymes EC 5.4.2.11, phosphoglycerate mutase (2,3-diphosphoglycerate-dependent) and EC 5.4.2.12, phosphoglycerate mutase (2,3-diphosphoglycerate-independent)
RECOMMENDED NAME
GeneOntology No.
phosphoglycerate mutase
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
knockdown of PGAM1 expression induces cancer cell apoptosis
metabolism
physiological function
-
the enzyme is involved in glycogen catabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-phospho-D-glycerate
3-phospho-D-glycerate
show the reaction diagram
3-phospho-D-glycerate
2-phospho-D-glycerate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
metavanadate
-
less than 5% residual activity at 10 mM after 10 min incubation
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-bisphosphoglyceric acid
-
dependent on 2,3-bisphosphoglyceric acid for activity
Cysteine hydrochloride
-
phosphoglycerate mutase activity requires the reductant dithiothreitol, cysteine hydrochloride, or glutathione for full activity
dithiothreitol
-
phosphoglycerate mutase activity requires the reductant dithiothreitol, cysteine hydrochloride, or glutathione for full activity
glutathione
-
phosphoglycerate mutase activity requires the reductant dithiothreitol, cysteine hydrochloride, or glutathione for full activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55 - 1.03
3-phospho-D-glycerate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
102 - 3200
3-phospho-D-glycerate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
186 - 228
3-phospho-D-glycerate
201
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.078
-
pH 7.5, 37C, activity in undialyzed cell extracts
71
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30C
225
in 30 mM Tris-HCl, 5 mM MgSO4, 20 mM KCl, pH 7.5, at 30C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
calculated from amino acid sequence
7.4
calculated from amino acid sequence
PDB
SCOP
CATH
ORGANISM
UNIPROT
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Mycobacterium leprae (strain Br4923)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
-
2 * 26000, calculated from amino acid sequence
56000
x * 56000, calculated from amino acid sequence
57000
x * 57000, calculated from amino acid sequence
102000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HiTrap nickel chelating column chromatography and HiTrap Q column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C2566/T7 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
phosphoglycerate mutase 1 is markedly upregulated in hepatocellular carcinoma
-
Show AA Sequence (503 entries)
Please use the Sequence Search for a specific query.