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The enzyme appears in viruses and cellular organisms
Synonyms
3-methylitaconate-delta-isomerase,
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(R)-3-methylitaconate isomerase
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3-methylitaconate-DELTA-isomerase
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Isomerase, methylitaconate
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Isomerase, methylitaconate DELTA-
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Methylitaconate isomerase
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methylitaconate = 2,3-dimethylmaleate
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intramolecular oxidoreduction
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isomerization
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methylitaconate DELTA2-DELTA3-isomerase
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itaconate
methylmaleate
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methylitaconate
2,3-dimethylmaleate
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r
Methylitaconate
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key step in the overall fermentation of nicotinate
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Methylitaconate
Dimethylmaleate
Methylitaconate
Dimethylmaleate
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Methylitaconate
Dimethylmaleate
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r
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Methylitaconate
Dimethylmaleate
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r
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Methylitaconate
Dimethylmaleate
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3-methylitaconate. The enzymatically active enantiomer has (R)-configuration
2,3-dimethylmaleate
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Methylitaconate
Dimethylmaleate
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formation of dimethylmaleate is favored
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methylitaconate
2,3-dimethylmaleate
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r
Methylitaconate
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key step in the overall fermentation of nicotinate
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additional information
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no metal ion required
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p-chloromercuriphenyl sulfonic acid
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EDTA
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10 mM, 30% increase in activity
mercaptan
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required for optimal activity
additional information
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enzyme contains sulfhydryl groups
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6.2
methylitaconate
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7
methylitaconate
(R,S)-3-methylitaconate
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1720
methylitaconate
(R,S)-3-methylitaconate
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additional information
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brenda
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UniProt
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not detectable in cells grown on glucose
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metabolism
3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway
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140000
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sucrose density gradient centrifugation
40270
theoretical, without Strep-tag
41500
theoretical, including the Strep-tag
45000
apparent molecular mass, determined by SDS-PAGE
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homotetramer
4 * 40270 Da
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the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A
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-20°C, protein concentration 1 mg/ml, little loss of activity after 60 days
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-80°C, protein concentration 10 mg/ml, less than 10% loss of activity after 6 months
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0°C, protein concentration 1 mg/ml, little loss of activity after 6 days
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affinity purification on a streptavidin column
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into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells
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Kung, H.F.; Tsai, L.
Nicotinic acid metabolism. VII. Mechanism of action of Clostridial alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase
J. Biol. Chem.
246
6436-6443
1971
Eubacterium barkeri
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Hartrampf, G.; Buckel, W.
On the steric course of the adenosylcobalamin-dependent 2-methyleneglutarate mutase reaction in Clostridium barkeri
Eur. J. Biochem.
156
301-304
1986
Eubacterium barkeri
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Kung, H.F.; Stadtman, T.C.
Nicotinic acid metabolism. VI. Purification and properties of alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase
J. Biol. Chem.
246
3378-3388
1971
Eubacterium barkeri
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Alhapel, A.; Darley, D.J.; Wagener, N.; Eckel, E.; Elsner, N.; Pierik, A.J.
Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri
Proc. Natl. Acad. Sci. USA
103
12341-12346
2006
Eubacterium barkeri
brenda
Velarde, M.; Macieira, S.; Hilberg, M.; Brker, G.; Tu, S.-M.; Golding, B.T.; Pierik, A.J.; Buckel, W.; Messerschmidt, A.
Crystal structure and putative mechanism of 3-methylitaconate-DELTA-isomerase from Eubacterium barkeri
J. Mol. Biol.
391
609-620
2009
Eubacterium barkeri (Q0QLE6), Eubacterium barkeri
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