Reference on EC 5.3.3.1 - steroid DELTA-isomerase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kawahara, F.S.; Wang, S.F.; Talalay, P.
The preparation and properties of crystalline DELTA5-3-ketosteroid isomerase
J. Biol. Chem.
237
1500-1506
1962
Comamonas testosteroni
Jones, J.B.; Ship, S.
Inhibition of the 3-ketosteroid DELTA5->DELTA4 isomerase of Pseudomonas testosteroni by some bromo-3-ketosteroid derivatives
Biochim. Biophys. Acta
258
800-809
1972
Comamonas testosteroni
Geynet, Ph.; Gallay, J.; Alfsen, A.
Mammalian 3-oxosteroid DELTA5-DELTA4-isomerase. Solubilization by calcium ions and kinetic characteristics toward C19 and C21 steroids
Eur. J. Biochem.
31
464-469
1972
Comamonas testosteroni
Ford, H.C.; Engel, L.L.
Purification and properties of the DELTA5-3-beta-hydroxysteroid dehydrogenase-isomerase system of sheep adrenal cortical microsomes
J. Biol. Chem.
249
1363-1368
1974
Ovis aries
Tivol, W.F.; Beckman, E.D.; Benisek, W.F.
Effect of protein concentration on the molecular weight of DELTA5-3-ketosteroid isomerase
J. Biol. Chem.
250
271-275
1975
Comamonas testosteroni
Benson, A.M.; Suruda, A.J.; Talalay, P.
Concentration-dependent association of DELTA5-3-ketosteroid isomerase of Pseudomonas testosteroni
J. Biol. Chem.
250
276-280
1975
Comamonas testosteroni
Cheetham, P.S.J.
The identification, properties, and immobilization of the 3beta-hydroxysteroid oxidase and 3-oxosteroid DELTA4-DELTA5-isomerase components of cholesterol oxidase from Nocardia rhodochrous
J. Appl. Biochem.
1
51-59
1979
Nocardia rhodochrous
-
Smith, S.B.; Richards, J.W.; Benisek, W.F.
The purification and characterization of DELTA5-3-ketosteroid isomerase from Pseudomonas putida, a cysteine-containing isomerase
J. Biol. Chem.
255
2678-2684
1980
Pseudomonas putida
Benson, A.M.; Suruda, A.J.; Barrack, E.R.; Talalay, P.
Steroid-transforming enzymes
Methods Enzymol.
34B
557-566
1974
Comamonas testosteroni
Westbrook, E.M.
Characterization of a hexagonal crystal form of an enzyme of steroid metabolism, DELTA5-3-ketosteroid isomerase: a new method of crystal density measurement
J. Mol. Biol.
103
659-664
1976
Comamonas testosteroni
Weintraub, H.; Vincent, F.; Baulieu, E.E.; Alfsen, A.
Interaction of steroids with Pseudomonas testosteroni 3-oxosteroid DELTA4-DELTA5 isomerase
Biochemistry
16
5045-5053
1977
Comamonas testosteroni
Smith, A.G.; Brooks, C.J.W.
The substrate specificity and stereochemistry, reversibility and inhibition of the 3-oxo steroid DELTA 4-DELTA 5-isomerase component of cholesterol oxidase
Biochem. J.
167
121-129
1977
Nocardia erythropolis
Penning, T.M.; Covey, D.F.; Talalay, P.
Inactivation of DELTA5-3-oxo steroid isomerase with active-site-directed acetylenic steroids
Biochem. J.
193
217-227
1981
Comamonas testosteroni
Penning, T.M.; Covey, D.F.; Talalay, P.
Irreversible inactivation of DELTA5-3-ketosteroid isomerase of Pseudomonas testosteroni by acetylenic suicide substrates. Mechanism of formation and properties of the steroid-enzyme adduct
J. Biol. Chem.
256
6842-6850
1981
Comamonas testosteroni
Hearne, M.; Benisek, W.F.
Use of a solid-phase photoaffinity reagent to label a steroid binding site: application to the DELTA5-3-ketosteroid isomerase of Pseudomonas testosteroni
Biochemistry
24
7511-7516
1985
Comamonas testosteroni
Talalay, P.; Wang, V.S.
Enzymic isomerization of DELTA5-3-ketosteroids
Biochim. Biophys. Acta
18
300-301
1955
Pseudomonas sp.
Westbrook, E.M.; Sigler, P.B.; Berman, H.; Glusker, J.P.; Bunick, G.; Benson, A.; Talalay, P.
Characterization of a monoclinic crystal form of an enzyme of steroid metabolism, DELTA5-3-ketosteroid isomerase
J. Mol. Biol.
103
665-667
1976
Comamonas testosteroni
Simard, J.; Melner, M.H.; Breton, N.; Low, K.G.; Zhao, H.F.; Periman, L.M.; Labrie, F.
Characterization of macaque 3beta-hydroxy-5-ene steroid dehydrogenase/DELTA5-DELTA4-isomerase: structure and expression in steroidogenic and peripheral tissues in primate
Mol. Cell. Endocrinol.
75
101-110
1991
Macaca mulatta
Thomas, J.L.; Berko, E.A.; Faustino, A.; Myers, R.P.; Strickler, R.C.
Human placental 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5-->4-ene-isomerase: purification from microsomes, substrate kinetics, and inhibition by product steroids
J. Steroid Biochem.
31
785-793
1988
Homo sapiens
Thomas, J.L.; Myers, R.P.; Strickler, R.C.
Human placental 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5-->4-ene-isomerase: purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymes
J. Steroid Biochem.
33
209-217
1989
Homo sapiens
Van Luu-The; Takahashi, M.; Labrie, F.
Differential inhibition of dehydrogenase and 5ene->4-ene isomerase activities of purified 3beta-hydroxysteroid dehydrogenase. Evidence for two distinct sites
J. Steroid Biochem. Mol. Biol.
40
545-548
1991
Homo sapiens
Brooks, B.; Benisek, W.F.
Mechanism of the reaction catalyzed by DELTA5-3-ketosteroid isomerase of Comamonas (Pseudomonas) testosteroni: kinetic properties of a modified enzyme in which tyrosine 14 is replaced by 3-fluorotyrosine
Biochemistry
33
2682-2687
1994
Comamonas testosteroni
Pollack, R.M.; Bantia, S.; Bounds, P.L.; Koffman, B.M.
pH-Dependence of the kinetic parameters for 3-oxo-DELTA5-steroid isomerase substrate catalysis and inhibition by (3S)-spiro[5alpha-androstane-3,2'-oxiran]-17-one
Biochemistry
25
1905-1911
1986
Comamonas testosteroni
Bevins, C.L.; Pollack, R.M.; Kayser, R.H.; Bounds, P.L.
Detection of transient enzyme-steroid complex during active-site-directed irreversible inhibition of 3-oxo-DELTA5-steroid isomerase
Biochemistry
25
5159-5164
1986
Comamonas testosteroni
Cherradi, N.; Guidicelli, C.; Defaye, G.; Chambaz, E.M.
Purification and characterization of 3beta-hydroxysteroid-dehydrogenase/isomerase from bovine adrenal cortex
J. Steroid Biochem. Mol. Biol.
41
831-836
1992
Bos taurus
Dumont, M.; van Luu-The; Dupont, E.; Pelletier, G.; Llabrie, F.
Characterization, expression, and immunohistochemical localization of 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase in human skin
J. Invest. Dermatol.
99
415-421
1992
Homo sapiens
Oh, B.H.; Kim, S.W.; Ryu, S.E.; Kim, S.S.; Choi, K.Y.
Crystallization and preliminary X-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B
Proteins
24
514-515
1996
Pseudomonas putida
Kim, S.W.; Cha, S.S.; Cho, H.S.; Kim, J.S.; Ha, N.C.; Cho, M.J.; Joo, S.; Kim, K.K.; Choi, K.Y.; Oh, B.H.
High-resolution crystal structures of delta5-3-ketosteroid isomerase with and without a reaction intermediate analogue [published erratum appears in Biochemistry 1998 May 5;37(18):6614]
Biochemistry
36
14030-14036
1997
Pseudomonas putida (P07445), Pseudomonas putida
Cho, H.S.; Choi, G.; Choi, K.Y.; Oh, B.H.
Crystal structure and enzyme mechanism of Delta 5-3-ketosteroid isomerase from Pseudomonas testosteroni
Biochemistry
37
8325-8330
1998
Comamonas testosteroni (P00947), Comamonas testosteroni
Benisek, W.F.
Labeling of DELTA-3-ketosteroid isomerase by photoexcited steroid ketones
Methods Enzymol.
46
469-479
1977
Comamonas testosteroni
Brandt, M.; Levy, M.A.
3beta-Hydroxy-DELTA5-steroid dehydrogenase/3-keto-DELTA5-steroid isomerase from bovine adrenals: mechanism of inhibition by 3-oxo-4-aza steroids and kinetic mechanism of the dehydrogenase
Biochemistry
28
140-148
1989
Bos taurus
Kayser, R.H.; Bounds, P.L.; Bevins, C.L.; Pollack, R.M.
Affinity alkylation of bacterial DELTA5-3-ketosteroid isomerase. Identification of the amino acid modified by steroidal 17beta-oxiranes
J. Biol. Chem.
258
909-915
1983
Comamonas testosteroni
Covey, D.F.; Robinson, C.H.
Conjugated allenic 3-oxo-5,10-secosteroids. Irreversible inhibitors of DELTA5-3-ketosteroid isomerase
J. Am. Chem. Soc.
98
5038-5040
1976
Comamonas testosteroni
Bazold, F.H.; Robinson, C.H.
Irreversible inhibition of DELTA5-3-ketosteroid isomerase by 5,10-secosteroids
J. Am. Chem. Soc.
97
2575-2578
1975
Comamonas testosteroni
-
Hearne, M.; Benisek, W.F.
Modification of DELTA5-3-ketosteroid isomerase induced by ultraviolet irradiation in the presence of the solid-phase photoaffinity reagent DELTA6-testosterone agarose
J. Protein Chem.
3
87-97
1984
Comamonas testosteroni
-
Ishii-Ohba, H.; Inano, H.; Tamaoki, B.
Purification and properties of testicular 3beta-hydroxy-5-ene-steroid dehydrogenase and 5-ene-4-ene isomerase
J. Steroid Biochem.
25
555-560
1986
Rattus norvegicus
Thomas, J.L.; Mason, J.I.; Blanco, G.; Veisaga, M.L.
The engineered, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase: purification, characterization and crystallization
J. Mol. Endocrinol.
27
77-83
2001
Homo sapiens
Wong, C.K.; Keung, W.M.
Bovine adrenal 3beta-hydroxysteroid dehydrogenase (E.C. 1.1.1.145)/5-ene-4-ene isomerase (E.C. 5.3.3.1): characterization and its inhibition by isoflavones
J. Steroid Biochem. Mol. Biol.
71
191-202
1999
Bos taurus
Kim, D.H.; Nam, G.H.; Jang, D.S.; Choi, G.; Joo, S.; Kim, J.S.; Oh, B.H.; Choi, K.Y.
Roles of active site aromatic residues in catalysis by ketosteroid isomerase from Pseudomonas putida biotype B
Biochemistry
38
13810-13819
1999
Pseudomonas putida
Kim, D.H.; Jang, D.S.; Nam, G.H.; Yun, S.; Cho, J.H.; Choi, G.; Lee, H.C.; Choi, K.Y.
Equilibrium and kinetic analysis of folding of ketosteroid isomerase from Comamonas testosteroni
Biochemistry
39
13084-13092
2000
Comamonas testosteroni
Henot, F.; Pollack, R.M.
Catalytic activity of the D38A mutant of 3-oxo-DELTA 5-steroid isomerase: recruitment of aspartate-99 as the base
Biochemistry
39
3351-3359
2000
Comamonas testosteroni
Kim, D.H.; Jang, D.S.; Nam, G.H.; Choi, G.; Kim, J.S.; Ha, N.C.; Kim, M.S.; Oh, B.H.; Choi, K.Y.
Contribution of the hydrogen-bond network involving a tyrosine triad in the active site to the structure and function of a highly proficient ketosteroid isomerase from Pseudomonas putida biotype B
Biochemistry
39
4581-4589
2000
Pseudomonas putida
Choi, G.; Ha, N.C.; Kim, S.W.; Kim, D.H.; Park, S.; Oh, B.H.; Choi, K.Y.
Asp-99 donates a hydrogen bond not to Tyr-14 but to the steroid directly in the catalytic mechanism of DELTA 5-3-ketosteroid isomerase from Pseudomonas putida biotype B
Biochemistry
39
903-909
2000
Pseudomonas putida
Choi, G.; Ha, N.C.; Kim, M.S.; Hong, B.H.; Oh, B.H.; Choi, K.Y.
Pseudoreversion of the catalytic activity of Y14F by the additional substitution(s) of tyrosine with phenylalanine in the hydrogen bond network of DELTA 5-3-ketosteroid isomerase from Pseudomonas putida biotype B
Biochemistry
40
6828-6835
2001
Pseudomonas putida
Kim, S.W.; Choi, K.Y.
Identification of active site residues by site-directed mutagenesis of DELTA5-3-ketosteroid isomerase from Pseudomonas putida biotype B
J. Bacteriol.
177
2602-2605
1995
Pseudomonas putida
Nam, G.H.; Kim, D.H.; Ha, N.C.; Jang do, S.; Yun, Y.S.; Hong, B.H.; Oh, B.H.; Choi, K.Y.
Contribution of conserved amino acids at the dimeric interface to the conformational stability and the structural integrity of the active site in ketosteroid isomerase from Pseudomonas putida biotype B
J. Biochem.
134
101-110
2003
Pseudomonas putida
Cho, H.S.; Ha, N.C.; Choi, G.; Kim, H.J.; Lee, D.; Oh, K.S.; Kim, K.S.; Lee, W.; Choi, K.Y.; Oh, B.H.
Crystal structure of DELTA(5)-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin settles the correct hydrogen bonding scheme for transition state stabilization
J. Biol. Chem.
274
32863-32868
1999
Comamonas testosteroni (P00947)
Yun, S.; Jang do, S.; Choi, G.; Kim, K.S.; Choi, K.Y.; Lee, H.C.
Trifluoroethanol increases the stability of DELTA(5)-3-ketosteroid isomerase. 15N NMR relaxation studies
J. Biol. Chem.
277
23414-23419
2002
Comamonas testosteroni
Yun, Y.S.; Lee, T.H.; Nam, G.H.; Jang, D.S.; Shin, S.; Oh, B.H.; Choi, K.Y.
Origin of the different pH activity profile in two homologous ketosteroid isomerases
J. Biol. Chem.
278
28229-28236
2003
Comamonas testosteroni (P00947), Comamonas testosteroni
Thomas, J.L.; Duax, W.L.; Addlagatta, A.; Brandt, S.; Fuller, R.R.; Norris, W.
Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase
J. Biol. Chem.
278
35483-35490
2003
Homo sapiens
Kim, D.H.; Nam, G.H.; Jang, D.S.; Yun, S.; Choi, G.; Lee, H.C.; Choi, K.Y.
Roles of dimerization in folding and stability of ketosteroid isomerase from Pseudomonas putida biotype B
Protein Sci.
10
741-752
2001
Pseudomonas putida
Jang do, S.; Cha, H.J.; Cha, S.S.; Hong, B.H.; Ha, N.C.; Lee, J.Y.; Oh, B.H.; Lee, H.S.; Choi, K.Y.
Structural double-mutant cycle analysis of a hydrogen bond network in ketosteroid isomerase from Pseudomonas putida biotype B
Biochem. J.
382
967-973
2004
Pseudomonas putida
Yonkunas, M.J.; Xu, Y.; Tang, P.
Anesthetic interaction with ketosteroid isomerase: Insights from molecular dynamics simulations
Biophys. J.
89
2350-2356
2005
Comamonas testosteroni
Soma, K.K.; Alday, N.A.; Hau, M.; Schlinger, B.A.
Dehydroepiandrosterone metabolism by 3beta-hydroxysteroid dehydrogenase/D5-D4 isomerase in adult zebra finch brain: sex difference and rapid effect of stress
Endocrinology
145
1668-1677
2004
Taeniopygia guttata
Soma, K.K.; Sinchak, K.; Lakhter, A.; Schlinger, B.A.; Micevych, P.E.
Neurosteroids and female reproduction: Estrogen increases 3beta-HSD mRNA and activity in rat hypothalamus
Endocrinology
146
4386-4390
2005
Rattus norvegicus (P22071)
Yun, Y.S.; Nam, G.H.; Kim, Y.G.; Oh, B.H.; Choi, K.Y.
Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B
FEBS J.
272
1999-2011
2005
Pseudomonas putida
Houck, W.J.; Pollack, R.M.
Temperature effects on the catalytic activity of the D38E mutant of 3-oxo-DELTA5-steroid isomerase: favorable enthalpies and entropies of activation relative to the nonenzymatic reaction catalyzed by acetate ion
J. Am. Chem. Soc.
126
16416-16425
2004
Comamonas testosteroni (P00947)
Thomas, J.L.; Boswell, E.L.; Scaccia, L.A.; Pletnev, V.; Umland, T.C.
Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3b-HSD1) for substrates, coenzymes, and inhibitors relative to human 3b-HSD2
J. Biol. Chem.
280
21321-21328
2005
Homo sapiens
Pletnev, V.Z.; Thomas, J.L.; Rhaney, F.L.; Holt, L.S.; Scaccia, L.A.; Umland, T.C.; Duax, W.L.
Rational proteomics V: structure-based mutagenesis has revealed key residues responsible for substrate recognition and catalysis by the dehydrogenase and isomerase activities in human 3beta-hydroxysteroid dehydrogenase/isomerase type 1
J. Steroid Biochem. Mol. Biol.
101
50-60
2006
Homo sapiens (P14060), Homo sapiens (P26439), Homo sapiens
Tam, H.; Schlinger, B.A.
Activities of 3beta-HSD and aromatase in slices of developing and adult zebra finch brain
Gen. Comp. Endocrinol.
150
26-33
2006
Taeniopygia guttata
Hutchinson, M.H.; Chase, H.A.
Refolding strategies for ketosteroid isomerase following insoluble expression in Escherichia coli
Biotechnol. Bioeng.
94
1089-1098
2006
Comamonas testosteroni (P00947)
Hutchinson, M.H.; Morreale, G.; Middelberg, A.P.; Chase, H.A.
Production of enzymatically active ketosteroid isomerase following insoluble expression in Escherichia coli
Biotechnol. Bioeng.
95
724-733
2006
Comamonas testosteroni (P00947)
Hutchinson, M.H.; Chase, H.A.
Intensified process for the purification of an enzyme from inclusion bodies using integrated expanded bed adsorption and refolding
Biotechnol. Prog.
22
1187-1193
2006
Comamonas testosteroni (P00947)
Jang, D.S.; Lee, H.J.; Lee, B.; Hong, B.H.; Cha, H.J.; Yoon, J.; Lim, K.; Yoon, Y.J.; Kim, J.; Ree, M.; Lee, H.C.; Choi, K.Y.
Detection of an intermediate during the unfolding process of the dimeric ketosteroid isomerase
FEBS Lett.
580
4166-4171
2006
Pseudomonas putida
Meffre, D.; Delespierre, B.; Gouezou, M.; Schumacher, M.; Stein, D.G.; Guennoun, R.
3beta-Hydroxysteroid dehydrogenase/5-ene-4-ene isomerase mRNA expression in rat brain: effect of pseudopregnancy and traumatic brain injury
J. Steroid Biochem. Mol. Biol.
104
293-300
2007
Rattus norvegicus (P22071)
Kraut, D.A.; Sigala, P.A.; Pybus, B.; Liu, C.W.; Ringe, D.; Petsko, G.A.; Herschlag, D.
Testing electrostatic complementarity in enzyme catalysis: hydrogen bonding in the ketosteroid isomerase oxyanion hole
PLoS Biol.
4
501-519
2006
Comamonas testosteroni, Pseudomonas putida
-
Sharma, K.; Vazquez-Ramirez, R.; Kubli-Garfias, C.
A theoretical model of the catalytic mechanism of the DELTA5-3-ketosteroid isomerase reaction
Steroids
71
549-557
2006
Pseudomonas sp.
Hunter, A.C.; Coyle, E.; Morse, F.; Dedi, C.; Dodd, H.T.; Koussoroplis, S.J.
Transformation of 5-ene steroids by the fungus Aspergillus tamarii KITA: Mixed molecular fate in lactonization and hydroxylation pathways with identification of a putative 3beta-hydroxy-steroid dehydrogenase/DELTA(5)-DELTA(4) isomerase pathway
Biochim. Biophys. Acta
1791
110-117
2008
Aspergillus tamarii, Aspergillus tamarii KITA
Aste, N.; Watanabe, Y.; Shimada, K.; Saito, N.
Sex- and age-related variation in neurosteroidogenic enzyme mRNA levels during quail embryonic development
Brain Res.
1201
15-22
2008
Coturnix japonica
Sigala, P.A.; Kraut, D.A.; Caaveiro, J.M.; Pybus, B.; Ruben, E.A.; Ringe, D.; Petsko, G.A.; Herschlag, D.
Testing geometrical discrimination within an enzyme active site: constrained hydrogen bonding in the ketosteroid isomerase oxyanion hole
J. Am. Chem. Soc.
130
13696-13708
2008
Comamonas testosteroni, Pseudomonas putida (P07445)
Wilde, T.C.; Blotny, G.; Pollack, R.M.
Experimental evidence for enzyme-enhanced coupled motion/quantum mechanical hydrogen tunneling by ketosteroid isomerase
J. Am. Chem. Soc.
130
6577-6585
2008
Comamonas testosteroni
Lee, H.J.; Yoon, Y.J.; Jang, D.S.; Kim, C.; Cha, H.J.; Hong, B.H.; Choi, K.Y.; Lee, H.C.
15N NMR relaxation studies of Y14F mutant of ketosteroid isomerase: the influence of mutation on backbone mobility
J. Biochem.
144
159-166
2008
Comamonas testosteroni
Lee, H.J.; Jang, do S.; Cha, H.J.; Moon, H.S.; Hong, B.H.; Choi, K.Y.; Lee, H.C.
NMR studies on the equilibrium unfolding of ketosteroid isomerase by urea
J. Biochem.
144
215-221
2008
Pseudomonas putida
Lee, H.J.; Moon, H.S.; Jang, do S.; Cha, H.J.; Hong, B.H.; Choi, K.Y.; Lee, H.C.
Probing the equilibrium unfolding of ketosteroid isomerase through xenon-perturbed (1)H- (15)N multidimensional NMR spectroscopy
J. Biomol. NMR
40
65-70
2008
Pseudomonas putida
Thomas, J.L.; Mack, V.L.; Glow, J.A.; Moshkelani, D.; Terrell, J.R.; Bucholtz, K.M.
Structure/function of the inhibition of human 3beta-hydroxysteroid dehydrogenase type 1 and type 2 by trilostane
J. Steroid Biochem. Mol. Biol.
111
66-73
2008
Homo sapiens
Chakravorty, D.; Soudackov, A.; Hammes-Schiffer, S.
Hybrid quantum/classical molecular dynamics simulations of the proton transfer reactions catalyzed by ketosteroid isomerase: Analysis of hydrogen bonding, conformational motions, and electrostatics
Biochemistry
48
10608-10619
2009
Comamonas testosteroni (P00947)
Sigala, P.; Caaveiro, J.; Ringe, D.; Petsko, G.; Herschlag, D.
Hydrogen bond coupling in the ketosteroid isomerase active site
Biochemistry
48
6932-6939
2009
Comamonas testosteroni, Pseudomonas putida (P07445)
Childs, W.; Boxer, S.G.
Proton affinity of the oxyanion hole in the active site of ketosteroid isomerase
Biochemistry
49
2725-2731
2010
Pseudomonas putida
Childs, W.; Boxer, S.G.
Solvation response along the reaction coordinate in the active site of ketosteroid isomerase
J. Am. Chem. Soc.
132
6474-6480
2010
Pseudomonas putida
Hanoian, P.; Hammes-Schiffer, S.
Water in the active site of ketosteroid isomerase
Biochemistry
50
6689-6700
2011
Pseudomonas putida (P07445)
Chakravorty, D.K.; Hammes-Schiffer, S.
Impact of mutation on proton transfer reactions in ketosteroid isomerase: insights from molecular dynamics simulations
J. Am. Chem. Soc.
132
7549-7555
2010
Comamonas testosteroni (P00947)
Tars, K.; Olin, B.; Mannervik, B.
Structural basis for featuring of steroid isomerase activity in alpha class glutathione transferases
J. Mol. Biol.
397
332-340
2010
Homo sapiens (Q16772), Homo sapiens
Ewald, W.; Werbin, H.; Chaikoff, I.L.
Evidence for the presence of 17-hydroxypregnenedione isomerase in beef adrenal cortex
Biochim. Biophys. Acta
111
306-12
1965
Bos taurus
Wojciechowicz, B.; Kotwica, G.; Kolakowska, J.; Franczak, A.
The activity and localization of 3beta-hydroxysteroid dehydrogenase/DELTA(5)-DELTA(4) isomerase and release of androstenedione and progesterone by uterine tissues during early pregnancy and the estrous cycle in pigs
J. Reprod. Dev.
59
49-58
2013
Sus scrofa
van der Kamp, M.W.; Chaudret, R.; Mulholland, A.J.
QM/MM modelling of ketosteroid isomerase reactivity indicates that active site closure is integral to catalysis
FEBS J.
280
3120-3131
2013
Comamonas testosteroni
Jha, S.K.; Ji, M.; Gaffney, K.J.; Boxer, S.G.
Site-specific measurement of water dynamics in the substrate pocket of ketosteroid isomerase using time-resolved vibrational spectroscopy
J. Phys. Chem. B
116
11414-11421
2012
Pseudomonas putida
Parker, M.; Osuna, S.; Bollot, G.; Vaddypally, S.; Zdilla, M.; Houk, K.; Schafmeister, C.
Acceleration of an aromatic claisen rearrangement via a designed spiroligozyme catalyst that mimics the ketosteroid isomerase catalytic dyad
J. Am. Chem. Soc.
136
3817-3827
2014
Pseudomonas putida (P07445)
Ito, M.; Brinck, T.
Novel approach for identifying key residues in enzymatic reactions Proton abstraction in ketosteroid isomerase
J. Phys. Chem. B
118
13050-13058
2014
Comamonas testosteroni
Jang, D.S.; Choi, G.; Cha, H.J.; Shin, S.; Hong, B.H.; Lee, H.J.; Lee, H.C.; Choi, K.Y.
Contribution of a low-barrier hydrogen bond to catalysis is not significant in ketosteroid isomerase
Mol. Cells
38
409-415
2015
Pseudomonas putida (P07445)
Cha, H.; Jang, D.; Jin, K.; Lee, H.; Hong, B.; Kim, E.; Kim, J.; Lee, H.; Choi, K.; Ree, M.
Three-dimensional structures of a wild-type ketosteroid isomerase and its single mutant in solution
Sci. Adv. Mat.
6
2325-2333
2014
Pseudomonas putida
-
Meitinger, N.; Munkert, J.; Maia de Pdua, R.; de Souza Filho, J.; Maid, H.; Bauer, W.; Braga, F.; Kreis, W.
The catalytic mechanism of the 3-ketosteroid isomerase of Digitalis lanata involves an intramolecular proton transfer and the activity is not associated with the 3beta-hydroxysteroid dehydrogenase activity
Tetrahedron Lett.
57
1567-1571
2016
Digitalis lanata
-
Currin, A.; Dunstan, M.S.; Johannissen, L.O.; Hollywood, K.A.; Vinaixa, M.; Jervis, A.J.; Swainston, N.; Rattray, N.J.W.; Gardiner, J.M.; Kell, D.B.; Takano, E.; Toogood, H.S.; Scrutton, N.S.
Engineering the missing link in biosynthetic (-)-menthol production bacterial isopulegone isomerase
ACS Catal.
8
2012-2020
2018
Pseudomonas putida (P07445)
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