Any feedback?
Information on EC 5.2.1.13 - prolycopene isomerase
for references in articles please use BRENDA:EC5.2.1.13Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
5.2.1.13 prolycopene isomeraseIUBMB Comments
Requires FADH2 . The enzyme is involved in carotenoid biosynthesis.
Specify your search results
Word Map
- 5.2.1.13
- phytoene
- all-trans-lycopene
- zeta-carotene
- strigolactones
- tangerine
- violaxanthin
-
carotenogenesis
-
xanthophyl
-
apocarotenoids
-
chromoplast
-
carotenogenic
-
beta-cyclase
-
cis-to-trans
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
crtiso, carotenoid isomerase, prolycopene isomerase, zmcrtiso1, cocrtiso1-ora, carotene cis-trans isomerase, cocrtiso1-orb, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
carotenoid isomerase
CRTISO
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
7,9,7',9'-tetracis-lycopene = all-trans-lycopene
the mechanism of isomerization relies on acid-base catalysis
-
7,9,7',9'-tetracis-lycopene = all-trans-lycopene
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
7,9,7',9'-tetracis-lycopene cis-trans-isomerase
Requires FADH2 [1]. The enzyme is involved in carotenoid biosynthesis.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
7,9,7',9'-tetracis-lycopene
prolycopene
-
-
all-trans-lycopene is the main lycopene species formed, accompanied by certain amounts of newly formed cis isomers
-
?
(5'Z)-gamma-carotene
(all-E)-gamma-carotene
-
-
-
?
(5'Z)-gamma-carotene
(all-E)-gamma-carotene
no activity with the wild-type, but with mutant 1-ORa462insCHH
-
-
?
(5'Z)-gamma-carotene
(all-E)-gamma-carotene
no activity with the wild-type, but with variant E450G
-
-
?
(5'Z)-gamma-carotene
(all-E)-gamma-carotene
wild-type and mutants I370T and A412S
-
-
?
(5'Z)-rubixanthin
(all-E)-rubixanthin
no activity with the wild-type, but with mutant 1-ORa462insCHH
-
-
?
(5'Z)-rubixanthin
(all-E)-rubixanthin
no activity with the wild-type, but with variant E450G
-
-
?
(5'Z)-rubixanthin
(all-E)-rubixanthin
wild-type and mutants I370T and A412S
-
-
?
(5Z,9Z,5'Z,9'Z)-lycopene
(all-E)-lycopene
-
-
-
?
(5Z,9Z,5'Z,9'Z)-lycopene
(all-E)-lycopene
no activity with the wild-type, but with mutant 1-ORa462insCHH
-
-
?
(5Z,9Z,5'Z,9'Z)-lycopene
(all-E)-lycopene
no activity with the wild-type, but with variant E450G
-
-
?
(5Z,9Z,5'Z,9'Z)-lycopene
(all-E)-lycopene
wild-type and mutants I370T and A412S
-
-
?
prolycopene
(all-E)-lycopene
-
-
-
?
prolycopene
(all-E)-lycopene
no activity with the wild-type, but with mutant 1-ORa462insCHH
-
-
?
prolycopene
(all-E)-lycopene
no activity with the wild-type, but with variant E450G
-
-
?
prolycopene
(all-E)-lycopene
wild-type and mutants I370T and A412S
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
the enzyme can not isomerize the 15-cis double bond of 9,15,9'-tri-cis-zeta-carotene
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
additional information
?
-
-
isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FADH2
-
requires the reduced cofactor for activity. The enzyme requires anaerobic conditions for activity. Km: 0.00055 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
the enzyme requires anaerobic conditions for activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
the transcript of Zmcrtiso1 increases throughout endosperm development to day 25 after pollination. Until 30 after pollination the level decreases
brenda
ZEBRA2 is predominantly expressed in mesophyll cells of mature leaves
brenda
highest expression in the leaf, followed by the pod and the panicle
brenda
highest expression in the leaf, followed by the pod and the panicle
brenda
additional information
-
in wild-type fruit, the mRNA levels of CRTISO increase 10fold during the breaker stage of fruit ripening. Expression of CRTISO in fruit of the mutant tangerine(mic) is similar to that in the wild type
brenda
ZEBRA2 is predominantly expressed in mesophyll cells of mature leaves
brenda
highest expression among the tissues tested. In plants carrying the Zebra leaf mutation, yellow and green striped leaves appear at the seedling stage and gradually turn pale green towards the end of the tillering stage
brenda
additional information
expressed in all tested tissues and organs, with the highest expression in the leaf, followed by the pod and the panicle. Lowest expression in stem and the root
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
additional information
malfunction
-
in the wild type, 7 days after the breaker stage, 75% of total carotenoids in ripe fruit consist of all-trans-lycopene and more than 15% are lycopene precursors (neurosporene, zeta-carotene, phytofluene, and phytoene). In fruit of the mutant tangerine3183, the major carotenoid accumulates is prolycopene, whereas lycopene precursors, mostly in the cis configuration, constitute the rest of the carotenoids
malfunction
mutation of the ZEBRA2 gene results in the accumulation of all-trans-lycopene precursor, prolycopene (7,9,7',9'-tetracis-lycopene), in dark-grown zebra2 tissues. Light-grown zebra2 mutant exhibits the characteristic zebra phenotype and decreases level of lutein
malfunction
the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids
malfunction
the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. A Gly-to-Glu amino acid substitution at position 450 in CoCRTISO1-ORb abolishes enzyme activity completely
malfunction
the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. Deletion of Cys-His-His at positions 462-464 in isozyme CoCRTISO1-ORa abolishes enzyme activity completely
physiological function
isozyme CoCRTISO1 is a key regulator of the accumulation of 5-cis-carotenoids in calendula petals
physiological function
mutation in Mhz5 blocks carotenoid biosynthesis, reduces abscisic acid accumulation, and promotes ethylene production in etiolated seedlings. Mutation or overexpression of Mhz5 alters the expression of ethylene-responsive genes. The Mhz5-mediated abscisic acid pathway acts downstream of ethylene signaling to inhibit root growth
physiological function
mutation leads to moderately increased tiller numbers, semi-dwarfism and leaf variegation. Prolycopene accumulates while all-trans-lycopene is barely detectable in the dark-grown Mit3 mutant seedlings. Content of lutein and beta-carotene and of 2'-5-epi-5-deoxystrigol is significant in the mutants
physiological function
overexpression in transgenic tobacco resultsin an increase of total carotenoids in the leaves with betaotene and lutein being the predominants
additional information
residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme
additional information
residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme
additional information
residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme
additional information
residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme
additional information
residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme
additional information
residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme
additional information
-
residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme
additional information
residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme
additional information
residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme
additional information
residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme
additional information
residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme
additional information
residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme
additional information
residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme
additional information
-
residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme
Show AA Sequence and Transmembrane Helices (983 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A412S
site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme
G450E
site-directed mutagenesis, the isozyme CoCRTISO1-ORb mutant is catalytically inactive
I370T
site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive
L581P
site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive
P584L
site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene CoCRTISO1-ORa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis
gene CoCRTISO1-ORb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis
gene CoCRTISO1-Y, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis
gene CoCRTISO2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis
gene CoCRTISO3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis
gene CoCRTISO4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ethylene induces Mhz5 expression, the production of neoxanthin, and abscisic acid accumulation in roots
in wild-type fruit, the mRNA levels of CRTISO increases 10fold during the breaker stage of fruit ripening. Expression of CRTISO in fruit of the mutant tangerine(mic) is similar to that in the wild type
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yu, Q.; Ghisla, S.; Hirschberg, J.; Mann, V.; Beyer, P.
Plant carotene cis-trans isomerase CRTISO: a new member of the FADred-dependent flavopreoteins catalyzing non-redox reactions
J. Biol. Chem.
286
8666-8676
2011
Solanum lycopersicum
brenda
Isaacson, T.; Ronen, G.; Zamir, D.; Hirschberg, J.
Cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of beta-carotene and xanthophylls in plants
Plant Cell
14
333-342
2002
Solanum lycopersicum
brenda
Chai, C.; Fang, J.; Liu, Y.; Tong, H.; Gong, Y.; Wang, Y.; Liu, M.;, Wang, Y.; Qian, Q.; Cheng, Z.; Chu, C.
ZEBRA2, encoding a carotenoid isomerase, is involved in photoprotection in rice
Plant Mol. Biol.
75
211-221
2011
Oryza sativa (B0L964), Oryza sativa
brenda
Li, Q.; Farre, G.; Naqvi, S.; Breitenbach, J.; Sanahuja, G.; Bai, C.; Sandmann, G.; Capell, T.; Christou, P.; Zhu, C.
Cloning and functional characterization of the maize carotenoid isomerase and beta-carotene hydroxylase genes and their regulation during endosperm maturation
Transgenic Res.
19
1053-1068
2010
Zea mays (C1JFF5), Zea mays
brenda
Kishimoto, S.; Ohmiya, A.
Carotenoid isomerase is key determinant of petal color of Calendula officinalis
J. Biol. Chem.
287
276-285
2012
Calendula officinalis (G9MAL3), Calendula officinalis (G9MAL4), Calendula officinalis (G9MAL5), Calendula officinalis (G9MAL6), Calendula officinalis (G9MAL7), Calendula officinalis (G9MAL8), Calendula officinalis
brenda
Yin, C.C.; Ma, B.; Collinge, D.P.; Pogson, B.J.; He, S.J.; Xiong, Q.; Duan, K.X.; Chen, H.; Yang, C.; Lu, X.; Wang, Y.Q.; Zhang, W.K.; Chu, C.C.; Sun, X.H.; Fang, S.; Chu, J.F.; Lu, T.G.; Chen, S.Y.; Zhang, J.S.
Ethylene responses in rice roots and coleoptiles are differentially regulated by a carotenoid isomerase-mediated abscisic acid pathway
Plant Cell
27
1061-1081
2015
Oryza sativa Japonica Group (Q2R2A6)
brenda
Liu, L.; Xie, T.; Peng, P.; Qiu, H.; Zhao, J.; Fang, J.; Patil, S.B.; Wang, Y.; Fang, S.; Chu, J.; Yuan, S.; Zhang, W.; Li, X.
Mutations in the MIT3 gene encoding a caroteniod isomerase lead to increased tiller number in rice
Plant Sci.
267
1-10
2018
Oryza sativa Japonica Group (Q2R2A6)
brenda
Zhao, J.; Fang, Y.; Kang, S.; Ruan, B.; Xu, J.; Dong, G.; Yan, M.; Hu, J.; Zeng, D.; Zhang, G.; Gao, Z.; Guo, L.; Qian, Q.; Zhu, L.
Identification and characterization of a new allele for ZEBRA LEAF 2, a gene encoding carotenoid isomerase in rice
South Afr. J. Bot.
95
102-111
2014
Oryza sativa Japonica Group (Q2R2A6)
-
brenda
Li, Z.; Ji, J.; Wang, G.
Cloning and characterization of a Lycium chinense carotenoid isomerase gene enhancing carotenoid accumulation in transgenic tobacco
Transact. Tianjin Univ.
21
468-476
2015
Lycium chinense (A0A140YIA2)
-
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 5.2.1.13)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
