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bifunctional UDP-4-keto-6-deoxy-D-glucose epimerase/reductase
E3VXL5
-
deoxythymidine diphosphate-4-dehydrorhamnose 3,5-epimerase
-
-
deoxythymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase
dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase
dTDP-4-dehydrorhamnose 3,5-epimerase
dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase
-
-
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
dTDP-4-keto-L-rhamnose 3,5-epimerase
-
-
-
-
dTDP-6-deoxy-D-xylo-4-hexulose 3',5'-epimerase
dTDP-L-rhamnose synthetase
-
-
-
-
Epimerase, thymidine diphospho-4-ketorhamnose 3,5-
-
-
-
-
TDP-4-keto-6-deoxy-D-glucose 3,5-epimerase
-
TDP-4-keto-L-rhamnose-3,5-epimerase
-
-
-
-
TDP-4-ketorhamnose 3,5-epimerase
-
-
-
-
TDP-6-deoxy-D-xylo-4-hexopyranosid-4-ulose 3,5-epimerase
-
Thymidine diphospho-4-ketorhamnose 3,5-epimerase
-
-
-
-
Agl13
-
Agl13
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
-
deoxythymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase
-
deoxythymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase
-
-
DnmU
-
dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase
-
dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
-
dTDP-4-dehydrorhamnose 3,5-epimerase
Q81TP1
-
dTDP-4-dehydrorhamnose 3,5-epimerase
Q81TP1
-
-
dTDP-4-dehydrorhamnose 3,5-epimerase
-
dTDP-4-dehydrorhamnose 3,5-epimerase
-
-
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
-
-
-
-
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
-
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
-
-
dTDP-6-deoxy-D-xylo-4-hexulose 3',5'-epimerase
-
-
dTDP-6-deoxy-D-xylo-4-hexulose 3',5'-epimerase
-
-
-
dTDP-6-deoxy-D-xylo-4-hexulose 3',5'-epimerase
-
-
dTDP-6-deoxy-D-xylo-4-hexulose 3',5'-epimerase
-
-
RfbC
Q81TP1
-
RmbC
-
RmlC
previously RfbD
RmlC
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
-
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
dTDP-4-dehydro-6-deoxy-D-glucose
?
-
enzyme in biosynthesis of dTDP-L-dihydrostreptose from dTDP-6-deoxy-D-xylo-4-hexosulose
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-6-deoxy-L-mannose
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-L-rhamnose
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
TDP-6-deoxy-D-xylo-4-hexosulose
?
TDP-6-deoxy-D-xylo-4-hexosulose
TDP-6-deoxy-L-lyxo-4-hexosulose
TDP-6-deoxy-D-xylo-hexopyranosid-4-ulose
TDP-L-rhamnose
conversion of TDP-6-deoxy-D-xylo-hexopyranosid-4-ulose to TDP-L-rhamnose, catalyzed by the two enzymes TDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase, RmlC, and TDP-deoxy-L-lyxo-4-hexulose reductase, RmlD, overview
-
-
?
UDP-4-dehydro-6-deoxy-D-glucose
UDP-4-dehydro-6-deoxy-L-mannose
E3VXL5
-
i.e. UDP-4-dehydro-L-rhamnose, product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
-
?
additional information
?
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
Q81TP1
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
Q81TP1
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
?, r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
?
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-6-deoxy-L-mannose
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-6-deoxy-L-mannose
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-6-deoxy-L-mannose
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-6-deoxy-L-mannose
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-L-rhamnose
-
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-L-rhamnose
-
the enzyme is involved in the dTDP-rhamnose synthesis pathway which consists of three steps: (i) the synthesis of dTDP-4-keto-6-deoxy-D-glucose from glucose 1-phosphate and dTDP, (ii) the conversion of dTDP-4-keto-6-deoxy-D-glucose to dTDP-4-keto-L-rhamnose by dTDP-4-dehydrorhamnose 3,5-epimerase and (iii) the reduction of dTDP-4-keto-L-rhamnose to dTDP-L-rhamnose. The mutant K3 strain, with a spontaneous mutation that results in lower cellulose production, shows deoxythymidine diphosphate-4-dehydrorhamnose 3,5-epimerase activity, while the wild-type does not, determined by 2D-gel electrophoresis. It is possible that in the wild-type, transcription of the gene responsible for the expression of dTDP-4-dehydrorhamnose 3,5-epimerase is repressed by a transcription factor, and that a mutation in the gene encoding the transcription factor has rendered it non-functional
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme in biosynthesis of the cell wall component 6-deoxy-L-talose
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme in biosynthesis of dTDP-L-rhamnose from dTDP-D-glucose
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme in biosynthesis of the cell wall component 6-deoxy-L-talose
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme is required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
TDP-6-deoxy-L-lyxo-4-hexosulose
-
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
TDP-6-deoxy-L-lyxo-4-hexosulose
-
-
-
?
additional information
?
-
mass spectrometric analysis of reaction products in the assay of coupled enzymes, RML-1, RML-2, RML-3, and RML-4. Reductase activity toward dTDP-4-keto-6-deoxyglucose is observed, but only when both RML-4-RML-5 and RML-3 are added to the reaction mixture in the presence ofNAD(P)H, RML-3 may catalyze the 3,5-epimerization reaction
-
-
?
additional information
?
-
-
mass spectrometric analysis of reaction products in the assay of coupled enzymes, RML-1, RML-2, RML-3, and RML-4. Reductase activity toward dTDP-4-keto-6-deoxyglucose is observed, but only when both RML-4-RML-5 and RML-3 are added to the reaction mixture in the presence ofNAD(P)H, RML-3 may catalyze the 3,5-epimerization reaction
-
-
?
additional information
?
-
-
the enzyme is involved in the biosynthesis of dTDP-L-rhamnose, which is an essential component of the bacterial cell wall
-
?
additional information
?
-
-
the enzyme is required for dTDP-L-rhamnose biosynthesis
-
?
additional information
?
-
-
third enzyme of dTDP-L-rhamnose pathway
-
?
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dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
dTDP-4-dehydro-6-deoxy-D-glucose
?
-
enzyme in biosynthesis of dTDP-L-dihydrostreptose from dTDP-6-deoxy-D-xylo-4-hexosulose
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose
dTDP-4-dehydro-L-rhamnose
-
the enzyme is involved in the dTDP-rhamnose synthesis pathway which consists of three steps: (i) the synthesis of dTDP-4-keto-6-deoxy-D-glucose from glucose 1-phosphate and dTDP, (ii) the conversion of dTDP-4-keto-6-deoxy-D-glucose to dTDP-4-keto-L-rhamnose by dTDP-4-dehydrorhamnose 3,5-epimerase and (iii) the reduction of dTDP-4-keto-L-rhamnose to dTDP-L-rhamnose. The mutant K3 strain, with a spontaneous mutation that results in lower cellulose production, shows deoxythymidine diphosphate-4-dehydrorhamnose 3,5-epimerase activity, while the wild-type does not, determined by 2D-gel electrophoresis. It is possible that in the wild-type, transcription of the gene responsible for the expression of dTDP-4-dehydrorhamnose 3,5-epimerase is repressed by a transcription factor, and that a mutation in the gene encoding the transcription factor has rendered it non-functional
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
TDP-6-deoxy-D-xylo-4-hexosulose
?
TDP-6-deoxy-D-xylo-hexopyranosid-4-ulose
TDP-L-rhamnose
conversion of TDP-6-deoxy-D-xylo-hexopyranosid-4-ulose to TDP-L-rhamnose, catalyzed by the two enzymes TDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase, RmlC, and TDP-deoxy-L-lyxo-4-hexulose reductase, RmlD, overview
-
-
?
additional information
?
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
Q81TP1
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
Q81TP1
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
?, r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
-
-
r
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
?
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-4-dehydro-beta-L-rhamnose
-
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
dTDP-6-deoxy-D-xylo-4-hexulose
dTDP-6-deoxy-L-lyxo-4-hexulose
-
third enzyme of the rhamnose pathway
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme in biosynthesis of the cell wall component 6-deoxy-L-talose
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme in biosynthesis of dTDP-L-rhamnose from dTDP-D-glucose
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme in biosynthesis of the cell wall component 6-deoxy-L-talose
-
-
?
TDP-6-deoxy-D-xylo-4-hexosulose
?
-
enzyme is required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine
-
-
?
additional information
?
-
-
the enzyme is required for dTDP-L-rhamnose biosynthesis
-
?
additional information
?
-
-
third enzyme of dTDP-L-rhamnose pathway
-
?
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1-(3-(5-allyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylsulfonyl)-propyl)-1H-benzo[d]imidazol-2(3H)-one
-
1-(3-(5-allyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
i.e. 77074, a SID 7975595 analogue
1-(3-(5-allyl-8-methyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
-
1-(3-(5-ethyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylsulfonyl)-propyl)-1H-benzo[d]imidazol-2(3H)-one
-
1-(3-(5-ethyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
i.e. SID 7975595, a competitive, fast-on rate, fully reversible inhibitor of RmlC. Increases the Km for TDP-6-deoxy-D-xylo-hexopyranosid-4-ulose. Cytotoxic SID 7975595 has an IC50 of approximately 0.075 mM in HAE cells
1-(3-(5-methyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
i.e. 77072, a SID 7975595 analogue
1-(3-(5-methylphenyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
i.e. 77070, a SID 7975595 analogue
1-(3-(5-propyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
i.e. 77071, a SID 7975595 analogue
1-(3-(5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
i.e. 77073, a SID 7975595 analogue
additional information
modeling of triazinoindol-benzimidazolones into the active site of RmlC, overview
-
additional information
-
modeling of triazinoindol-benzimidazolones into the active site of RmlC, overview
-
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0.0004
1-(3-(5-allyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylsulfonyl)-propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
pH 7.4, 25°C
0.00012
1-(3-(5-allyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
-
0.02
1-(3-(5-allyl-8-methyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
-
0.0008
1-(3-(5-ethyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylsulfonyl)-propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
pH 7.4, 25°C
0.0002
1-(3-(5-ethyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
pH 7.4, 25°C
0.0005
1-(3-(5-methyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
pH 7.4, 25°C
0.0029
1-(3-(5-methylphenyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
pH 7.4, 25°C
0.00125
1-(3-(5-propyl-5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
pH 7.4, 25°C
0.0033
1-(3-(5H-[1,2,4]triazino[5,6-b]indol-3-ylthio)propyl)-1H-benzo[d]imidazol-2(3H)-one
Mycobacterium tuberculosis
pH 7.4, 25°C
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evolution
Archaea synthesize nucleotide-activated rhamnose by a pathway similar to that employed by Bacteria and distinct from that used by Eukarya and viruses
evolution
Q81TP1
structural comparison of the enzyme structure with RfbC homologues shows that the key active-site residues are conserved across kingdoms. RmlC-like epimerases belongs to the diverse cupin superfamily
evolution
-
structural comparison of the enzyme structure with RfbC homologues shows that the key active-site residues are conserved across kingdoms. RmlC-like epimerases belongs to the diverse cupin superfamily
-
evolution
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
Archaea synthesize nucleotide-activated rhamnose by a pathway similar to that employed by Bacteria and distinct from that used by Eukarya and viruses
-
malfunction
rhamnose and N-acetyl-galactosamine are not detected, and fucose is greatly diminished in the rmlC mutant (transposon pLOF/Sp inserted into rmlC (TDP-4-keto-6-deoxy-D-glucose 3,5-epimerase) compared with that in the wild-type strain)
malfunction
disruption of rmbC results in fragmented mycelia that quickly convert into gray pigmented spores
malfunction
the expression of the rhamnose biosynthetic genes by RNAi results in significant reductions in dTDP-rhamnose, but has no effect on the biosynthesis of a closely related sugar, ascarylose, found in the ascaroside pheromones. RNAi against rml-3 does not affect embryonic or larval development
malfunction
-
disruption of rmbC results in fragmented mycelia that quickly convert into gray pigmented spores
-
metabolism
-
dTDP-4-dehydrorhamnose 3,5-epimerase is an enzyme which may be associated with the cellulose synthesis-reducing effect of the mutation in strain K3
metabolism
the enzyme is required for L-rhamnose synthesis
metabolism
the enzyme from the doxorubicin biosynthesis gene cluster is involved in the biosynthesis of dTDP-L-daunosamine, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene rmbC. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other
metabolism
the enzyme is involved in the biosynthesis of dTDP-L-rhamnose, a precursor of cell wall biosynthesis, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene dnmU. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other
metabolism
Archaea synthesize nucleotide-activated rhamnose by a pathway similar to that employed by Bacteria and distinct from that used by Eukarya and viruses. The pathwayto synthesize nucleotide-activated rhamnose involves Agl11 as a glucose-1-phosphate thymidylyltransferase, Agl12 as a dTDP-glucose-4,6-dehydratase, Agl13 as a dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase, and Agl14 as a dTDP-4-dehydrorhamnose reductase
metabolism
biosynthesis of dTDP-L-rhamnose: RML-1 activates glucose 1-phosphate in the presence of either dTTP or UTP to yield dTDP-glucose or UDP-glucose, respectively. RML-2 is a dTDP-glucose 4,6-dehydratase, converting dTDP-glucose into dTDP-4-keto-6-deoxyglucose. And coincubation of dTDP-4-keto-6-deoxyglucose with RML-3 (3,5-epimerase) and RML-4 (4-keto-reductase) produces dTDP-rhamnose. A co-regulated protein, RML-5, forms a complex with RML-4. Rhamnose biosynthesis may play an important role in hypodermal development or the production of the cuticle or surface coat during molting
metabolism
Q81TP1
the biosynthetic pathway used to form the activated L-rhamnose donor dTDP-L-rhamnose consists of four enzymes, RfbA, RfbB, RfbC and RfbD. RfbC catalyzes the third reaction, a double epimerization producing dTDP-6-deoxy-L-lyxo-4-hexulose (dTDP-4-dehydro-L-rhamnose)
metabolism
the enzyme is involved in the dTDP-rhamnose biosynthetic pathway
metabolism
-
the biosynthetic pathway used to form the activated L-rhamnose donor dTDP-L-rhamnose consists of four enzymes, RfbA, RfbB, RfbC and RfbD. RfbC catalyzes the third reaction, a double epimerization producing dTDP-6-deoxy-L-lyxo-4-hexulose (dTDP-4-dehydro-L-rhamnose)
-
metabolism
-
the enzyme is involved in the biosynthesis of dTDP-L-rhamnose, a precursor of cell wall biosynthesis, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene dnmU. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other
-
metabolism
-
the enzyme from the doxorubicin biosynthesis gene cluster is involved in the biosynthesis of dTDP-L-daunosamine, pathway overview. It does not compensate for the other dTDP-4-keto-6-deoxyglucose 3,5-epimerase encoded by gene rmbC. Although dnmU and rmbC encode for similar functional proteins, their native roles in their respective biosynthetic pathways in vivo are specific and independent of one other
-
metabolism
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
Archaea synthesize nucleotide-activated rhamnose by a pathway similar to that employed by Bacteria and distinct from that used by Eukarya and viruses. The pathwayto synthesize nucleotide-activated rhamnose involves Agl11 as a glucose-1-phosphate thymidylyltransferase, Agl12 as a dTDP-glucose-4,6-dehydratase, Agl13 as a dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase, and Agl14 as a dTDP-4-dehydrorhamnose reductase
-
physiological function
the rmlC mutant strain colonizes the rabbit mitral valves approximately 3fold less effectively than the wild type strain
physiological function
enzyme Agl13 is a dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase
physiological function
Q81TP1
the exosporium layer of Bacillus anthracis spores is rich in L-rhamnose, a common bacterial cell-wall component, which often contributes to the virulence of pathogens by increasing their adherence and immune evasion. The biosynthetic pathway used to form the activated L-rhamnose donor dTDP-L-rhamnose consists of four enzymes, RfbA, RfbB, RfbC and RfbD. The dTDP-4-dehydrorhamnose 3,5-epimerase, RfbC, epimerizes dTDP-4-dehydro-6-deoxy-alpha-D-glucose into dTDP-4-dehydro-beta-L-rhamnose
physiological function
-
the exosporium layer of Bacillus anthracis spores is rich in L-rhamnose, a common bacterial cell-wall component, which often contributes to the virulence of pathogens by increasing their adherence and immune evasion. The biosynthetic pathway used to form the activated L-rhamnose donor dTDP-L-rhamnose consists of four enzymes, RfbA, RfbB, RfbC and RfbD. The dTDP-4-dehydrorhamnose 3,5-epimerase, RfbC, epimerizes dTDP-4-dehydro-6-deoxy-alpha-D-glucose into dTDP-4-dehydro-beta-L-rhamnose
-
physiological function
Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2
-
enzyme Agl13 is a dTDP-4-dehydro-6-deoxy-glucose-3,5-epimerase
-
additional information
Q81TP1
two different ligands are bound in the protein structure: diphosphate in the active site of one monomer and dTDP in the other monomer, active site structure, overview
additional information
-
two different ligands are bound in the protein structure: diphosphate in the active site of one monomer and dTDP in the other monomer, active site structure, overview
additional information
-
two different ligands are bound in the protein structure: diphosphate in the active site of one monomer and dTDP in the other monomer, active site structure, overview
-
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Wahl, H.P.; Grisebach, H.
Biosynthesis of streptomycin. dTDP-dihydrostreptose synthase from Streptomyces griseus and dTDP-4-keto-L-rhamnose 3,5-epimerase from S. griseus and Escherichia coli Y10
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Escherichia coli, Escherichia coli Y10
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Two enzymes in Streptomyces griseus for the synthesis of dTDP-L-dihydrostreptose from dTFP-6-deoxy-D-xylo-4-hexosulose
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Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar typhimurium LT2
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Methanothermobacter thermautotrophicus
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RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase
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Salmonella enterica
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High-resolution structures of RmlC from Streptococcus suis in complex with substrate analogs locate the active site of this class of Enzyme
Structure
11
715-723
2003
Streptococcus suis
brenda
Thuy, T.T.T.; Lee, H.C.; Kim, C.G.; Heide, L.; Sohng, J.K.
Functional characterizations of novWUS involved in novobiocin biosynthesis from Streptomyces spheroides
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436
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An enzyme module system for the synthesis of dTDP-activated deoxysugars from dTMP and sucrose
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Salmonella enterica
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2007
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Pseudomonas aeruginosa, Salmonella enterica
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Identification of triazinoindol-benzimidazolones as nanomolar inhibitors of the Mycobacterium tuberculosis enzyme TDP-6-deoxy-D-xylo-4-hexopyranosid-4-ulose 3,5-epimerase (RmlC)
Bioorg. Med. Chem.
18
896-908
2010
Mycobacterium tuberculosis (P9WH11), Mycobacterium tuberculosis
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Nguyen, V.; Flanagan, B.; Mikkelsen, D.; Ramirez, S.; Rivas, L.; Gidley, M.; Dykes, G.
Spontaneous mutation results in lower cellulose production by a Gluconacetobacter xylinus strain from Kombucha
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80
338-344
2010
Komagataeibacter xylinus
-
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Glycosylation of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans is dependent upon the lipopolysaccharide biosynthetic pathway
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Aggregatibacter actinomycetemcomitans (O05369)
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Exploration of two epimerase homologs in Streptomyces peucetius ATCC 27952
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97
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Streptomyces peucetius (C5J048), Streptomyces peucetius (O33707), Streptomyces peucetius ATCC 27952 (C5J048), Streptomyces peucetius ATCC 27952 (O33707)
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Shornikov, A.; Tran, H.; Macias, J.; Halavaty, A.S.; Minasov, G.; Anderson, W.F.; Kuhn, M.L.
Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose 3,5-epimerase (RfbC)
Acta Crystallogr. Sect. F
73
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2017
Bacillus anthracis (Q81TP1), Bacillus anthracis, Bacillus anthracis Ames (Q81TP1)
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Feng, L.; Shou, Q.; Butcher, R.A.
Identification of a dTDP-rhamnose biosynthetic pathway that oscillates with the molting cycle in Caenorhabditis elegans
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473
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Caenorhabditis elegans (Q17993), Caenorhabditis elegans
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Kaminski, L.; Eichler, J.
Haloferax volcanii N-glycosylation delineating the pathway of dTDP-rhamnose biosynthesis
PLoS ONE
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Haloferax volcanii (D4GU69), Haloferax volcanii, Haloferax volcanii WR536 / H53 / ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2 (D4GU69)
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