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Information on EC 4.5.1.1 - DDT-dehydrochlorinase
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4.5.1.1 DDT-dehydrochlorinaseSpecify your search results
Word Map
- 4.5.1.1
- hexachlorocyclohexane
-
dehydrochlorination
-
dehalogenase
- lindane
-
dechlorinase
- gamma-hexachlorocyclohexane
-
1,1,1-trichloro-2,2-bis-p-chlorophenylethane
- haloalkane
- nitroreductase
-
housefly
-
gamma-hch
- biotechnology
- 2,6-dinitrotoluene
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
dehydrochlorinase, ddtase, ddt dehydrochlorinase, ddt-dehydrohalogenase, ddt-dehydrochlorinase, gste2-2, gste5-5, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DDT-ase
-
-
-
-
DDT-dehydrohalogenase
DDTase
dehydrochlorinase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
elimination
-
-
of HCl, C-Cl bond cleavage
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane chloride-lyase [1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene-forming]
-
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CAS REGISTRY NUMBER
COMMENTARY
9031-20-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
1,1-dichloro-2,2-bis(4-chlorophenyl)ethane
1-chloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
i.e. TDE, reaction velocity is 4 times the rate of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane, i.e. DDT
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
the reaction requires the presence of GSH
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
the reaction requires the presence of GSH
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
the reaction requires the presence of GSH
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
1,1,1-trichloro-2,2-di-(p-chlorophenyl)ethane, i.e. p,p'-DDT
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
i.e. 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane, i.e. DDT in the presence of GSH
i.e. 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene i.e. DDE
ir
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
1,1,1-trichloro-2,2-di-(p-chlorophenyl)ethane, i.e. p,p'-DDT
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl
-
-
i.e. 1,1-dichloro-2,2-di-(p-chlorophenyl)ethylene, i.e. p,p'-DDE
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
-
-
-
?
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Mg2+
Zn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
p-chloromercuribenzoate
-
i.e. PCMB. 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane completely protects the fully aggregated enzyme against PCMB inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Thai mosquito, glutathione S-transferase isozyme 4c has enzymic activity
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
additional information
physiological function
-
the enzyme is involved in the catalytic degradation of 4,4'-DDT by eliminating either HCl or Cl- to form DDD/DDE
physiological function
-
the enzyme is involved in the catalytic degradation of 4,4'-DDT by eliminating either HCl or Cl- to form DDD/DDE
-
additional information
-
serine and tryptophan appear to be involved at the active site
additional information
-
serine and tryptophan appear to be involved at the active site
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GSTT1_DROER
209
0
23716
Swiss-Prot
other Location (Reliability: 2)
GSTT1_DROMA
200
0
22567
Swiss-Prot
other Location (Reliability: 3)
GSTT1_DROSE
209
0
23767
Swiss-Prot
other Location (Reliability: 2)
GSTT1_DROSI
209
0
23755
Swiss-Prot
other Location (Reliability: 2)
GSTT1_DROTE
200
0
22595
Swiss-Prot
other Location (Reliability: 3)
GSTD1_DROME
209
0
23866
Swiss-Prot
other Location (Reliability: 2)
GST1C_ANOGA
209
0
23783
Swiss-Prot
other Location (Reliability: 2)
GST1D_ANOGA
209
0
23445
Swiss-Prot
other Location (Reliability: 2)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
tetramer
trimer
additional information
tetramer
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane induces aggregation of the enzyme into the active tetrameric form
tetramer
-
dissociation of the tetrameric enzyme by mercaptoethanol or dithiothreitol
tetramer
-
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane induces aggregation of the enzyme into the active tetrameric form
-
tetramer
-
dissociation of the tetrameric enzyme by mercaptoethanol or dithiothreitol
-
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
side-chain modification
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
organic solvents
-
instability of the enzyme to organic solvents at temperatures about -5°C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
biotechnology
biotechnology
-
positive correlation of enzyme activity and resistance against DDT
biotechnology
-
positive correlation of enzyme activity and resistance against DDT, and also between enzyme activity and GST activity
biotechnology
-
positive correlation of enzyme activity and resistance against DDT, non-correlation between enzyme activity and GST activity
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lipke, H.; Kearns, C.W.
DDT dehydrochlorinase. I. Isolation, chemical properties, and spectrophotometric assay
J. Biol. Chem.
234
2123-2128
1959
Musca domestica
brenda
Lipke, H.; Kearns, C.W.
DDT dehydrochlorinase. II. Substrate and cofactor specificity
J. Biol. Chem.
234
2129-2132
1959
Musca domestica
brenda
Ishida, M.
Comparative studies on BHC metabolizing enzymes, DDT dehydrochlorinase and glutathione S-transferases
Agric. Biol. Chem.
32
947-955
1968
Musca domestica, Musca domestica RD4
-
brenda
Dinamarca, M.L.; Levenbook, L.; Valdes, E.
DDT-dehydrochlorinase. II. Subunits, sulfhydryl groups, and chemical composition
Arch. Biochem. Biophys.
147
374-383
1971
Musca domestica, Musca domestica P2/sel
brenda
Chadwick, R.W.; Chang, J.; Forehand, L.R.; Long, J.E.; Duffy, M.C.
Effect of lindane on intestinal nitroreductase, azoreductase, beta-glucuronidase, dechlorinase, and dehydrochlorinase activity
Pestic. Biochem. Physiol.
38
48-56
1990
Rattus norvegicus
-
brenda
Chang, J.; Chadwick, R.W.; Allison, J.C.; Hayes, Y.O.; Talley, D.L.; Autry, C.E.
Microbial succession and intestinal enzyme activities in the developing rat
J. Appl. Bacteriol.
77
709-718
1994
Rattus norvegicus
brenda
Ranson, H.; Rossiter, L.; Ortelli, F.; Jensen, B.; Wang, X.; Roth, C.W.; Collins, F.H.; Hemingway, J.
Identification of a novel class of insect glutathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae
Biochem. J.
359
295-304
2001
Anopheles gambiae
brenda
Prapanthadara, L.; Promtet, N.; Koottathep, S.; Somboon, P.; Ketterman, A.J.
Isoenzymes of glutathione S-transferase from the mosquito Anopheles dirus species B: the purification, partial characterization and interaction with various insecticides
Insect Biochem. Mol. Biol.
30
395-403
2000
Anopheles dirus
brenda
Prapanthadara, L.; Koottathep, S.; Promtet, N.; Suwonkerd, W.; Ketterman, A.J.; Somboon, P.
Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand
Southeast Asian J. Trop. Med. Public Health
31
111-118
2000
Culex quinquefasciatus, Anopheles minimus, Anopheles annularis
-
brenda
Latha, R.; Mandappa, I.; Thakur, M.; Manonmani, H.
Effect of denaturants on the structure and activity of DDT-dehydrochlorinase
Asian J. Microbiol. Biotechnol. Environ. Sci.
13
51-57
2011
Pseudomonas putida, Pseudomonas putida T5
-
brenda
Lumjuan, N.; Rajatileka, S.; Changsom, D.; Wicheer, J.; Leelapat, P.; Prapanthadara, L.A.; Somboon, P.; Lycett, G.; Ranson, H.
The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides
Insect Biochem. Mol. Biol.
41
203-209
2011
Aedes aegypti
brenda
Rangachary, L.; Rajagopalan, R.P.; Singh, T.M.; Krishnan, M.H.
Purification and characterization of DDT-dehydrohalogenase from Pseudomonas putida T5
Prep. Biochem. Biotechnol.
42
60-76
2012
Pseudomonas putida, Pseudomonas putida T5
brenda
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