We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments This microbial enzyme is involved in the degradation of ureidoglycolate, an intermediate of purine degradation. Not to be confused with EC 3.5.1.116, ureidoglycolate amidohydrolase, which releases ammonia rather than urea.
Word Map
4.3.2.3
pituitary
neuropeptide
cooh-terminal
glycine-extended
endoproteolytic
alpha-hydroxylating
1.14.17.3
prohormone
neurointermediate
corticotrope
ascorbate-dependent
glucuronyl
granule-associated
cuproenzymes
beta-monooxygenase
allantoicase
uteroglobin
The enzyme appears in viruses and cellular organisms
Synonyms
peptidylglycine alpha-amidating monooxygenase, ugl, peptidyl-alpha-hydroxyglycine alpha-amidating lyase, ureidoglycolate lyase, ureidoglycolase, ureidoglycolate urea-lyase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(-)-ureidoglycolate urea-lyase
lyase, ureidoglycolate
-
-
-
-
peptidyl-alpha-hydroxyglycine alpha-amidating lyase
-
-
peptidylglycine alpha-amidating monooxygenase
-
-
ureidoglycolatase
-
-
-
-
ureidoglycolate hydrolase
ureidoglycolate lyase
-
-
ureidoglycolate lyase, releasing urea
ureidoglycolate urea-lyase
-
-
(-)-ureidoglycolate urea-lyase
-
-
-
-
(-)-ureidoglycolate urea-lyase
-
-
allA
-
UGL
-
-
-
-
ureidoglycolate hydrolase
-
-
-
-
ureidoglycolate hydrolase
-
ureidoglycolate hydrolase
-
-
-
ureidoglycolate lyase, releasing urea
-
ureidoglycolate lyase, releasing urea
-
YbbT
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-ureidoglycolate = glyoxylate + urea
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
elimination
-
-
elimination
-
-
C-N-bond cleavage
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-ureidoglycolate urea-lyase (glyoxylate-forming)
This microbial enzyme is involved in the degradation of ureidoglycolate, an intermediate of purine degradation. Not to be confused with EC 3.5.1.116, ureidoglycolate amidohydrolase, which releases ammonia rather than urea.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-benzoyl-alpha-hydroxyglycine
glyoxylate + benzamide
-
2% of activity with (S)-ureidoglycolate
-
?
(S)-cinnamoyl-alpha-hydroxyglycine
glyoxylate + cinnamamide
-
5.5% of activity with (S)-ureidoglycolate
-
?
(S)-ureidoglycolate
glyoxylate + urea
1-naphtylacetyl-alpha-hydroxyglycine
glyoxylate + 1-naphtaleneacetamide
-
2.3% of activity with (S)-ureidoglycolate
-
?
3-phenylpropionyl-alpha-hydroxyglycine
glyoxylate + 3-phenylpropylacetamide
-
1.0% of activity with (S)-ureidoglycolate
-
?
4'-methoxyphenylacetyl-alpha-hydroxyglycine
glyoxylate + 4'-methoxyphenylacetamide
-
1.3% of activity with (S)-ureidoglycolate
-
?
4'-nitrophenylacetyl-alpha-hydroxyglycine
glyoxylate + 4'-nitrophenylacetamide
-
0.5% of activity with (S)-ureidoglycolate
-
?
benzoyl-alpha-hydroxyglycine
glyoxylate + benzamide
-
-
-
-
?
cinnamoyl-alpha-hydroxyglycine
glyoxylate + cinnamamide
-
-
-
-
?
phenylacetyl-alpha-hydroxyglycine
glyoxylate + phenylacetamide
-
0.8% of activity with (S)-ureidoglycolate
-
?
ureidoglycolate
glyoxylate + urea
additional information
?
-
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
nickel-dependent urea-release activity
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
nickel-dependent urea-release activity
-
-
?
ureidoglycolate
?
-
induced by urate
-
-
?
ureidoglycolate
?
-
enzyme is involved in purine degradation
-
-
?
ureidoglycolate
?
-
-
-
-
?
ureidoglycolate
?
-
enzyme is involved in allantoin degradation
-
-
?
ureidoglycolate
?
-
enzyme is involved in purine degradation
-
-
?
ureidoglycolate
?
-
enzyme is involved in degradation of xanthine
-
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
r
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
-
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
ureidoglycolate
glyoxylate + urea
-
?
-
?
additional information
?
-
recombinant enzyme from Escherichia coli is unable to release ammonia directly from ureidoglycolate
-
-
?
additional information
?
-
-
recombinant enzyme from Escherichia coli is unable to release ammonia directly from ureidoglycolate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-ureidoglycolate
glyoxylate + urea
ureidoglycolate
glyoxylate + urea
-
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
nickel-dependent urea-release activity
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
-
-
-
-
?
(S)-ureidoglycolate
glyoxylate + urea
nickel-dependent urea-release activity
-
-
?
ureidoglycolate
?
-
induced by urate
-
-
?
ureidoglycolate
?
-
enzyme is involved in purine degradation
-
-
?
ureidoglycolate
?
-
-
-
-
?
ureidoglycolate
?
-
enzyme is involved in allantoin degradation
-
-
?
ureidoglycolate
?
-
enzyme is involved in purine degradation
-
-
?
ureidoglycolate
?
-
enzyme is involved in degradation of xanthine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
-
activation
Mn2+
-
activates
Mn2+
-
2fold increase in activity
Ni2+
-
activates
Ni2+
nickel-dependent urea-release activity
Ni2+
nickel-dependent urea-release activity
Zn2+
-
activation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Fe2+
-
strong inactivation
N-acetylphenylpyruvate
-
-
EDTA
-
complete inhibition, 50% of activity is restored after removal of EDTA, addition of Mn2+, Zn2+ or Mg2+ recover the activity to the initial levels
EDTA
complete inhibition, the activity can only be rescued by an excess of Ni2+ ions, not by other metal ions, i.e. Ca2+, Co2+, Cu2+, Mg2+, Mn2+, Zn2+, and Fe2+
EDTA
-
10 mM, complete inhibition, activity can be restored by Mn2+ and Co2+, slightly by Zn2+
Ni2+
-
strong inactivation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Carcinoma
Amidation inhibitors 4-phenyl-3-butenoic acid and 5-(acetylamino)-4-oxo-6-phenyl-2-hexenoic acid methyl ester are novel HDAC inhibitors with anti-tumorigenic properties.
Carcinoma
Androgen-independent expression of adrenomedullin and peptidylglycine alpha-amidating monooxygenase in human prostatic carcinoma.
Carcinoma
Isolation and functional expression of human pancreatic peptidylglycine alpha-amidating monooxygenase.
Coma
Hyaluronic acid derived from other streptococci supports Streptococcus pneumoniae in vitro biofilm formation.
Glioblastoma
Neutralization of adrenomedullin inhibits the growth of human glioblastoma cell lines in vitro and suppresses tumor xenograft growth in vivo.
Glioma
Neutralization of adrenomedullin inhibits the growth of human glioblastoma cell lines in vitro and suppresses tumor xenograft growth in vivo.
Menkes Kinky Hair Syndrome
In vitro copper stimulation of plasma peptidylglycine alpha-amidating monooxygenase in Menkes disease variant with occipital horns.
Multiple Sclerosis
Increased peptidylglycine alpha-amidating monooxygenase activity in cerebrospinal fluid of patients with multiple sclerosis.
Neoplasms
Molecular analysis integrating different pathways associated with androgen-independent progression in LuCaP 23.1 xenograft.
Pheochromocytoma
Identification of potential gene markers and insights into the pathophysiology of pheochromocytoma malignancy.
Pheochromocytoma
Regulation of carboxypeptidase E by membrane depolarization in PC12 pheochromocytoma cells: comparison with mRNAs encoding other peptide- and catecholamine-biosynthetic enzymes.
Pituitary Neoplasms
Peptidylglycine alpha-amidating monooxygenase (PAM) immunoreactivity and messenger RNA in human pituitary and increased expression in pituitary tumours.
Prostatic Neoplasms
Catalytically active peptidylglycine alpha-amidating monooxygenase in the media of androgen-independent prostate cancer cell lines.
Seizures
Interactions of peptide amidation and copper: Novel biomarkers and mechanisms of neural dysfunction.
Thyroid Neoplasms
Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.006
(-)-ureidoglycolate
-
30°C, pH 7.8, in the presence of 0.5 mM Mn2+
0.5
(S)-ureidoglycolate
-
30°C, pH 7.0
0.03
1-naphtylacetyl-alpha-hydroxyglycine
-
30°C, pH 7.0
0.04
3-phenylpropionyl-alpha-hydroxyglycine
-
30°C, pH 7.0
0.09
4'-nitrophenylacetyl-alpha-hydroxyglycine
-
30°C, pH 7.0
0.028
4-methoxyphenylacetyl-alpha-hydroxyglycine
-
30°C, pH 7.0
0.09
benzoyl-alpha-hydroxyglycine
-
30°C, pH 7.0
0.025
cinnamoyl-alpha-hydroxyglycine
-
30°C, pH 7.0
0.024
phenylacetyl-alpha-hydroxyglycine
-
30°C, pH 7.0
0.33 - 17
ureidoglycolate
0.33
ureidoglycolate
-
-
3.9
ureidoglycolate
-
50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.018
ureidoglycolate
-
50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00056
Acetopyruvate
-
30°C, pH 7.0
0.0000022
benzoylpyruvate
-
30°C, pH 7.0
11.6
maleamic acid
-
30°C, pH 7.0
0.0012
N-acetylphenylpyruvate
-
30°C, pH 7.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.439
-
50 mM Tes, pH 7.8, 0.5 mM MnSO4, 3 mM phenylhydrazine
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.5 - 8.6
-
pH 6.5: about 50% of maximal activity, pH 8.6: about 40% of maximal activity
6 - 9
-
-
6 - 9
-
pH 6.0: about 40% of maximal activity, pH 9.0: about 20% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
70
-
and above, chemical instability of ureidoglycolate at higher temperatures
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30 - 70
-
approx. 30% of maximal activity at 40°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
chickpea
-
-
brenda
-
-
-
brenda
-
-
-
brenda
O157:H7 EDL933
-
-
brenda
-
-
-
brenda
-
-
-
brenda
W200
-
-
brenda
W200
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
mackerel
-
-
brenda
-
-
-
brenda
-
SwissProt
brenda
gene allA
SwissProt
brenda
O157:H7 EDL933
-
-
brenda
french bean
-
-
brenda
L. cv. Great Northern
-
-
brenda
-
-
-
brenda
gene dal3
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
developing pods
brenda
-
-
brenda
-
-
brenda
-
-
brenda
additional information
-
all tissues
brenda
-
-
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
-
detoxification of the reaction product glyoxylate in the peroxisomes by the malate synthase reaction
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
malfunction
DAL3 deletion abolishes the activity on ureidoglycolate
additional information
the AllA structure reveals two gracious small barrel (cupin) folds
additional information
-
the AllA structure reveals two gracious small barrel (cupin) folds
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
121000
-
sucrose density gradient centrifugation
21727
-
x * 21727, calculation from nucleotide sequence
31000
-
6 * 31000, SDS-PAGE
33000
-
2 * 33000, SDS-PAGE
38000
-
6 * 38000, SDS-PAGE
64000
-
-
64000
-
sucrose density gradient centrifugation
64000
-
2 * 64000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 21727, calculation from nucleotide sequence
dimer
-
2 * 33000, SDS-PAGE
dimer
-
2 * 64000, SDS-PAGE
hexamer
-
6 * 31000, SDS-PAGE
hexamer
-
6 * 38000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.7 A resolution, sitting drops method
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S767A
-
mutant is engineered to lack the N-glycosylation site at Asn765
Y582F
-
mutant is engineered for analysing the role of the thyrosine residues for the activity of the enzyme
Y595F
-
mutant is engineered for analysing the role of the thyrosine residues for the activity of the enzyme
Y654F
-
mutant is engineered for analysing the role of the thyrosine residues for the activity of the enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
70
-
15 min, in presence of Mn2+, complete inactivation
80
-
no loss of activity after 60 min in the presence of Mn2+
90
-
complete loss of activity after 40 min in the presence of Mn2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 10 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 10% glycerol, stable for at least 3 months
-
-20°C, stable for 3 months
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ammonium sulfate precipitation, gel filtration, heat treatment, hydrophobic interaction chromatography, immunoaffinity chromatography
-
ammonium sulfate, gel filtration, heat treatment, ion exchange, electrophoresis
-
DEAE-Sephacel, oxamate affinity, Superose
-
Ni-NTA affinity chromatography
-
-
partial
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression as a C-terminal His6-tag fusion protein
gene allA, encoded in the ALL locus
gene DAL3, the DAL metabolic gene cluster, the so called degradation of allantoin locus, is located on chromosome IX
into the pCIS vector for transfection of CHO cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
the locus encoding the enzyme is expressed under anaerobiosis and nitrogen starvation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Takada, Y.; Tsukiji, N.
Peroxisomal localization and activation by bivalent metal ions of ureidoglycolate lyase, the enzyme involved in urate degradation in Candida tropicalis
J. Bacteriol.
169
2284-2286
1987
Candida tropicalis
brenda
Takada, Y.; Noguchi, T.
Ureidoglycollate lyase, a new metalloenzyme of peroxisomal urate degradation in marine fish liver
Biochem. J.
235
391-397
1986
Scomber japonicus, Sardinops melanostictus
brenda
Kaspari, H.; Busse, W.
Oxidative degradation of purines by the facultative phototrophic bacterium Rhodopseudomonas capsulata
Arch. Microbiol.
144
67-70
1986
Rhodobacter capsulatus
-
brenda
Machida, Y.; Nakanishi, T.
Aerobic degradation of purines by Enterobacter cloacae
Agric. Biol. Chem.
46
2135-2136
1982
Enterobacter cloacae
-
brenda
Elzainy, T.A.; El-Awamry, Z.A.
Utilization and degradation of xanthine by Streptomyces viridiviolaceus
Zentralbl. Mikrobiol.
137
133-137
1982
Streptomyces viridiviolaceus
-
brenda
Trijbels, F.; Vogels, G.D.
Allantoate and ureidoglycolate degradation by Pseudomonas aeruginosa
Biochim. Biophys. Acta
132
115-126
1967
Pseudomonas aeruginosa
brenda
Choi, K.S.; Lee, K.W.; Roush, A.H.
The assay of yeast ureidoglycolatase
Anal. Biochem.
17
413-422
1966
Cyberlindnera jadinii, Saccharomyces cerevisiae
brenda
Fujiwara, S.; Noguchi, T.
Degradation of purines: only ureidoglycollate lyase out of four allantoin-degrading enzymes is present in mammals
Biochem. J.
312
315-318
1995
Rattus norvegicus
brenda
Yamaguchi, Y.
Ureidoglycollate lyase
J. Kyushu Dent. Soc.
51
29-40
1997
Bos taurus, Gallus gallus, Platyrrhini, Rattus norvegicus, Rhodobacter capsulatus
-
brenda
Wells, X.E.; Lees, E.M.
Ureidoglycolate amidohydrolase from developing French bean fruits (Phaseolus vulgaris [L.].)
Arch. Biochem. Biophys.
287
151-159
1991
Phaseolus vulgaris
brenda
Yoo, H.; Cooper, T.G.
The ureidoglycollate hydrolase (DAL3) gene in Saccharomyces cerevisiae
Yeast
7
693-698
1991
Saccharomyces cerevisiae
brenda
Falkinham, J.O.; George, K.L.; Parker, B.C.; Gruft, H.
Uric acid utilization by Mycobacterium intracellulare and Mycobacterium scrofulaceum isolates
J. Bacteriol.
155
36-39
1983
Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium scrofulaceum W200
brenda
McIninch, J.K.; McIninch, J.D.; May, S.W.
Catalysis, stereochemistry, and inhibition of ureidoglycolate lyase
J. Biol. Chem.
278
50091-50100
2003
Burkholderia cepacia
brenda
Munoz, A.; Piedras, P.; Aguilar, M.; Pineda, M.
Urea is a product of ureidoglycolate degradation in chickpea. Purification and characterization of the ureidoglycolate urea-lyase
Plant Physiol.
125
828-834
2001
Cicer arietinum
brenda
Munoz, A.; Raso, M.J.; Pineda, M.; Piedras, P.
Degradation of ureidoglycolate in French bean (Phaseolus vulgaris) is catalysed by a ubiquitous ureidoglycolate urea-lyase
Planta
224
175-184
2006
Phaseolus vulgaris
brenda
Raymond, S.; Tocilj, A.; Ajamian, E.; Li, Y.; Hung, M.; Matte, A.; Cygler, M.
Crystal structure of ureidoglycolate hydrolase (AllA) from Escherichia coli O157:H7
Proteins
61
454-459
2005
Escherichia coli, Escherichia coli (P63486), Escherichia coli O157:H7 EDL933
brenda
De, M.; Bell, J.; Blackburn, N.J.; Mains, R.E.; Eipper, B.A.
Role for an essential tyrosine in peptide amidation
J. Biol. Chem.
281
20873-20882
2006
Rattus norvegicus
brenda
Percudani, R.; Carnevali, D.; Puggioni, V.
Ureidoglycolate hydrolase, amidohydrolase, lyase: how errors in biological databases are incorporated in scientific papers and vice versa
Database (Oxford)
2013
bat071
2013
Saccharomyces cerevisiae (P32459), Saccharomyces cerevisiae, Escherichia coli (P63486), Escherichia coli
brenda
Select items on the left to see more content.
html completed