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dihydroxyacetone phosphate
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
glycerone phosphate
2-oxopropanal + phosphate
glycerone phosphate
methylglyoxal + phosphate
glycerone phosphate
pyruvaldehyde + phosphate
-
tautomerization to methylglyoxal
-
?
additional information
?
-
dihydroxyacetone phosphate
?
-
first enzyme in the reaction sequence for the conversion of dihydroxyacetone phosphate to pyruvate, may play a role in the control of glycolysis
-
-
?
dihydroxyacetone phosphate
?
-
during elevated metabolism, the synthesis of methylglyoxal from dihydroxyacetone phosphate temporarily relieves the cells from stress caused by phosphorylated intermediates and allows the cells to grow for a limited time. If during this period the environment changes, e.g. the level of the carbon source is reduced, the cells are not only able to survive but are also able to colonize their environment
-
-
?
dihydroxyacetone phosphate
?
-
the enzyme plays an important role in the catabolism of the triose phosphates
-
-
?
dihydroxyacetone phosphate
?
-
first enzyme in the reaction sequence for the conversion of dihydroxyacetone phosphate to pyruvate, may play a role in the control of glycolysis
-
-
?
dihydroxyacetone phosphate
?
-
the enzyme is involved in the methylglyoxal by-pass
-
-
?
dihydroxyacetone phosphate
?
-
constitutive enzyme, may be involved in by-pass sequence for part of the Embden-Meyerhof glycolytic pathway
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
strictly specific for
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
the true product of the enzymatic reaction is the enol form of methylglyoxal which is ketonized in solution
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
catalyzes the elimination reaction of DHAP, which leads to phosphate and the enol of methylglyoxal, which is subsequently tautomerized to methylglyoxal in solution
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
converts dihydrocyacetone phosphate to a cis-enediolic intermediate, then catalyses the elimination of phosphate to form the enol of methylglyoxal
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
converts dihydrocyacetone phosphate to enol pyruvaldehyde, this enol then tautomerizes to methylglyoxal in solution
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
intermediate is enol pyruvaldehyde is stereospecifically formed
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
ir
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
strictly specific for
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
since the enzyme accepts the trans-enediol-phosphate as a substrate, it is likely that it or the trans-enediolate is an intermediate in the catalytic reaction with dihydroxyacetone phosphate
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
strictly specific for
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
dihydroxyacetone phosphate
methylglyoxal + phosphate
-
-
-
?
glycerone phosphate
2-oxopropanal + phosphate
-
-
-
?
glycerone phosphate
2-oxopropanal + phosphate
-
-
-
?
glycerone phosphate
2-oxopropanal + phosphate
-
-
-
?
glycerone phosphate
methylglyoxal + phosphate
-
-
-
?
glycerone phosphate
methylglyoxal + phosphate
-
the unregulated production of methylglyoxal appears to be due to a rapid increase in the glycolysis intermediates from ribose degradation. Such a metabolic burden may result in methylglyoxal production by methylglyoxal synthase
-
-
?
additional information
?
-
enzyme dos no catalyze the elimination reaction with glyceraldehyde phosphate
-
?
additional information
?
-
enzyme is not capable of abstracting the C2 proton from glyceraldehyde phosphate, which is an absolute requirement for the function of MGS
-
?
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0.08 - 6.3
dihydroxyacetone phosphate
0.3 - 1.2
glycerone phosphate
0.08
dihydroxyacetone phosphate
mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C
0.09
dihydroxyacetone phosphate
-
-
0.19
dihydroxyacetone phosphate
-
enzyme form II
0.2
dihydroxyacetone phosphate
-
wild-type enzyme
0.2
dihydroxyacetone phosphate
pH 7, 25°C, wild-type
0.21
dihydroxyacetone phosphate
mutant carrying C-terminal extension YQRYLADRLK, pH 6.0, 60°C, Hill coefficient 1.5
0.21
dihydroxyacetone phosphate
mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C
0.22
dihydroxyacetone phosphate
-
-
0.23
dihydroxyacetone phosphate
-
enzyme form I
0.25
dihydroxyacetone phosphate
mutant carrying C-terminal extension YQRYLADRLK C-terminal extension YQRYLADRLK plus mutation D10N, pH 6.0, 60°C, Hill coefficient 1.5
0.26
dihydroxyacetone phosphate
pH 7, 25°C, H98N mutant
0.41
dihydroxyacetone phosphate
mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.47
dihydroxyacetone phosphate
-
-
0.5
dihydroxyacetone phosphate
-
-
0.5
dihydroxyacetone phosphate
wild-type, Hill coefficient 1, pH 6.5, 60°C
0.5
dihydroxyacetone phosphate
wild-type, pH 6.0, 60°C, Hill coefficient 1.5
0.53
dihydroxyacetone phosphate
-
-
0.6
dihydroxyacetone phosphate
pH 7, 25°C, H98Q mutant, presence of phosphoglycolate
0.62
dihydroxyacetone phosphate
pH 6, 25°C, wild-type
0.67
dihydroxyacetone phosphate
carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C
0.75
dihydroxyacetone phosphate
mutant D10N, pH 6.0, 60°C, Hill coefficient 1.5
0.76
dihydroxyacetone phosphate
-
-
0.8
dihydroxyacetone phosphate
wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C
0.83
dihydroxyacetone phosphate
wild-type, Hill coefficient 2.2, pH 6.2, 60°C
1.03
dihydroxyacetone phosphate
mutant V101A, Hill coefficient 1, pH 6.5, 60°C
1.17
dihydroxyacetone phosphate
wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C
1.25
dihydroxyacetone phosphate
mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C
1.3
dihydroxyacetone phosphate
pH 7, 25°C, H98Q mutant, absence of phosphoglycolate
1.35
dihydroxyacetone phosphate
-
mutant lacking ten amino acids from C-terminal tail, Hill coefficient 1.3, pH 7.0, 60°C
1.38
dihydroxyacetone phosphate
mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C
1.44
dihydroxyacetone phosphate
mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C
1.45
dihydroxyacetone phosphate
carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C
1.5
dihydroxyacetone phosphate
mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C
1.6
dihydroxyacetone phosphate
mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C
1.65
dihydroxyacetone phosphate
mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C
1.7
dihydroxyacetone phosphate
mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C
1.7
dihydroxyacetone phosphate
mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C
1.81
dihydroxyacetone phosphate
mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C
2.4
dihydroxyacetone phosphate
mutant G56A, Hill coefficient 2, pH 6.5, 60°C
2.7
dihydroxyacetone phosphate
mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C
2.74
dihydroxyacetone phosphate
-
wild-type, Hill coefficient 1, pH 7.0, 60°C
2.76
dihydroxyacetone phosphate
mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C
2.76
dihydroxyacetone phosphate
mutant S55D, Hill coefficient 1, pH 6.5, 60°C
2.79
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C
2.8
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C
3
dihydroxyacetone phosphate
-
-
3.2
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C
3.27
dihydroxyacetone phosphate
mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C
3.5
dihydroxyacetone phosphate
mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C
5.03
dihydroxyacetone phosphate
mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C
6.3
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.3
glycerone phosphate
mutant enzyme D100V, at pH 6.2 and 60°C
0.5
glycerone phosphate
mutant enzyme R80D/D100R, at pH 6.2 and 60°C
0.56
glycerone phosphate
allosteric enzyme
0.8
glycerone phosphate
mutant enzyme R8Q, at pH 6.2 and 60°C
0.8
glycerone phosphate
wild type enzyme, at pH 6.2 and 60°C
0.9
glycerone phosphate
mutant enzyme R80K, at pH 6.2 and 60°C
1
glycerone phosphate
mutant enzyme D100N, at pH 6.2 and 60°C
1.2
glycerone phosphate
mutant enzyme R80I, at pH 6.2 and 60°C
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0.00003 - 338
dihydroxyacetone phosphate
8 - 325
glycerone phosphate
0.00003
dihydroxyacetone phosphate
mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.00005
dihydroxyacetone phosphate
mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C
0.0001
dihydroxyacetone phosphate
mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.00013
dihydroxyacetone phosphate
mutant G56A, Hill coefficient 2, pH 6.5, 60°C
0.00025
dihydroxyacetone phosphate
mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C
0.00025
dihydroxyacetone phosphate
mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C
0.0004
dihydroxyacetone phosphate
mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C
0.001
dihydroxyacetone phosphate
mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C
0.09
dihydroxyacetone phosphate
mutant carrying C-terminal extension YQRYLADRLK C-terminal extension YQRYLADRLK plus mutation D10N, pH 6.0, 60°C, Hill coefficient 1.5
0.37
dihydroxyacetone phosphate
mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.41
dihydroxyacetone phosphate
mutant D10N, pH 6.0, 60°C, Hill coefficient 1.5
0.5
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C
0.55
dihydroxyacetone phosphate
mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.62
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.7
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C
0.75
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C
0.75
dihydroxyacetone phosphate
mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C
0.96
dihydroxyacetone phosphate
pH 7, 25°C, H98Q mutant, absence of phosphoglycolate
1
dihydroxyacetone phosphate
mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C
1.1
dihydroxyacetone phosphate
mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C
1.4
dihydroxyacetone phosphate
mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C
2.1
dihydroxyacetone phosphate
-
mutant lacking ten amino acids from C-terminal tail, Hill coefficient 1.3, pH 7.0, 60°C
4.4
dihydroxyacetone phosphate
pH 7, 25°C, H98N mutant
6.08
dihydroxyacetone phosphate
pH 7, 25°C, H98Q mutant, absence of phosphoglycolate
17
dihydroxyacetone phosphate
pH 7, 25°C, H98Q mutant, presence of phosphoglycolate
23.1
dihydroxyacetone phosphate
-
wild-type, Hill coefficient 1, pH 7.0, 60°C
76
dihydroxyacetone phosphate
mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C
100
dihydroxyacetone phosphate
mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C
103
dihydroxyacetone phosphate
mutant carrying C-terminal extension YQRYLADRLK, pH 6.0, 60°C, Hill coefficient 1.5
138
dihydroxyacetone phosphate
carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C
153
dihydroxyacetone phosphate
carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C
179
dihydroxyacetone phosphate
pH 6, 25°C, wild-type
220
dihydroxyacetone phosphate
-
wild-type enzyme
220
dihydroxyacetone phosphate
pH 7, 25°C, wild-type
220
dihydroxyacetone phosphate
wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C
230
dihydroxyacetone phosphate
wild-type, Hill coefficient 2.2, pH 6.2, 60°C
233
dihydroxyacetone phosphate
wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C
240
dihydroxyacetone phosphate
mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C
260
dihydroxyacetone phosphate
mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C
300
dihydroxyacetone phosphate
mutant S55D, Hill coefficient 1, pH 6.5, 60°C
326
dihydroxyacetone phosphate
wild-type, Hill coefficient 1, pH 6.5, 60°C
326
dihydroxyacetone phosphate
wild-type, pH 6.0, 60°C, Hill coefficient 1.5
327
dihydroxyacetone phosphate
wild-type, Hill coefficient 1, pH 6.5, 60°C
338
dihydroxyacetone phosphate
mutant V101A, Hill coefficient 1, pH 6.5, 60°C
8
glycerone phosphate
mutant enzyme R8Q, at pH 6.2 and 60°C
13
glycerone phosphate
mutant enzyme D100V, at pH 6.2 and 60°C
15
glycerone phosphate
mutant enzyme R80I, at pH 6.2 and 60°C
29
glycerone phosphate
mutant enzyme D100N, at pH 6.2 and 60°C
38
glycerone phosphate
mutant enzyme R80K, at pH 6.2 and 60°C
162
glycerone phosphate
mutant enzyme R80D/D100R, at pH 6.2 and 60°C
218
glycerone phosphate
wild type enzyme, at pH 6.2 and 60°C
325
glycerone phosphate
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00004 - 653
dihydroxyacetone phosphate
9.8 - 324
glycerone phosphate
0.00004
dihydroxyacetone phosphate
mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.00004
dihydroxyacetone phosphate
mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C
0.00005
dihydroxyacetone phosphate
mutant G56A, Hill coefficient 2, pH 6.5, 60°C
0.00009
dihydroxyacetone phosphate
mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.00015
dihydroxyacetone phosphate
mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C
0.00016
dihydroxyacetone phosphate
mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C
0.0003
dihydroxyacetone phosphate
mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C
0.0006
dihydroxyacetone phosphate
mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C
0.1
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.14
dihydroxyacetone phosphate
mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.2
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C
0.22
dihydroxyacetone phosphate
mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.27
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C
0.3
dihydroxyacetone phosphate
mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C
0.374
dihydroxyacetone phosphate
mutant carrying C-terminal extension YQRYLADRLK C-terminal extension YQRYLADRLK plus mutation D10N, pH 6.0, 60°C, Hill coefficient 1.5
0.4
dihydroxyacetone phosphate
mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C
0.52
dihydroxyacetone phosphate
mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C
0.544
dihydroxyacetone phosphate
mutant D10N, pH 6.0, 60°C, Hill coefficient 1.5
0.86
dihydroxyacetone phosphate
mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C
1.6
dihydroxyacetone phosphate
-
mutant lacking ten amino acids from C-terminal tail, Hill coefficient 1.3, pH 7.0, 60°C
8.4
dihydroxyacetone phosphate
-
wild-type, Hill coefficient 1, pH 7.0, 60°C
12
dihydroxyacetone phosphate
mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C
79
dihydroxyacetone phosphate
mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C
95
dihydroxyacetone phosphate
carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C
110
dihydroxyacetone phosphate
mutant S55D, Hill coefficient 1, pH 6.5, 60°C
130
dihydroxyacetone phosphate
mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C
180
dihydroxyacetone phosphate
mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C
190
dihydroxyacetone phosphate
wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C
227
dihydroxyacetone phosphate
carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C
275
dihydroxyacetone phosphate
wild-type, Hill coefficient 2.2, pH 6.2, 60°C
288
dihydroxyacetone phosphate
wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C
330
dihydroxyacetone phosphate
mutant V101A, Hill coefficient 1, pH 6.5, 60°C
490
dihydroxyacetone phosphate
mutant carrying C-terminal extension YQRYLADRLK, pH 6.0, 60°C, Hill coefficient 1.5
490
dihydroxyacetone phosphate
mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C
653
dihydroxyacetone phosphate
wild-type, Hill coefficient 1, pH 6.5, 60°C
653
dihydroxyacetone phosphate
wild-type, pH 6.0, 60°C, Hill coefficient 1.5
9.8
glycerone phosphate
mutant enzyme R8Q, at pH 6.2 and 60°C
12.5
glycerone phosphate
mutant enzyme R80I, at pH 6.2 and 60°C
30
glycerone phosphate
mutant enzyme D100N, at pH 6.2 and 60°C
42
glycerone phosphate
mutant enzyme R80K, at pH 6.2 and 60°C
43
glycerone phosphate
mutant enzyme D100V, at pH 6.2 and 60°C
272
glycerone phosphate
wild type enzyme, at pH 6.2 and 60°C
324
glycerone phosphate
mutant enzyme R80D/D100R, at pH 6.2 and 60°C
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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D101E
-
reduced ratio of turnover-numer:Km-value by about 10000fold compared to the wild-type enzyme
D101N
-
reduced ratio of turnover-numer:Km-value by about 10000fold compared to the wild-type enzyme
D71E
-
reduced ratio of turnover-numer:Km-value by about 1000fold compared to the wild-type enzyme
H98N
50fold decrease in kcat
H98Q
250fold lower catalytic activity than wild-type enzyme, change in conformation
D10N
almost complete loss of activity
DELTAR97
strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 3.3
G56A
significant increase in Hill coefficient, large decrease in turnover number
G56S
significant increase in Hill coefficient, large decrease in turnover number
R97E
strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 3.4
S55D
mutant has lost its allostery
S55K
increase in sensitivity to phosphate
V101A
mutant has lost its allostery
V101I
mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency
V101S
mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency
D100N
the mutant shows reduced catalytic efficiency compared to the wild type enzyme
D100V
the mutant shows reduced catalytic efficiency compared to the wild type enzyme
R80D/D100R
the mutant shows increased catalytic efficiency compared to the wild type enzyme
R80I
the mutant shows reduced catalytic efficiency compared to the wild type enzyme
R80K
the mutant shows reduced catalytic efficiency compared to the wild type enzyme
R80Q
the mutant shows reduced catalytic efficiency compared to the wild type enzyme
Q62R
inactive
D71N
-
reduced ratio of turnover-numer:Km-value by about 1000fold compared to the wild-type enzyme
D71N
-
2500fold decrease in kcat
R97K
mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency
R97K
strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 1.5
additional information
-
mutant lacking ten amino acids from C-terminal tail shows homotropic cooperative behavior in presence of dihydroxyacetone phosphate and is not only more flexible but also less stable compared to wild-type
additional information
a mutant carrying one additional His residue between residues Arg22 and His23 shos remarkable increase in thermostability, whereas a mutant carrying two additional His residues between residues Arg22 and His23 is very unstable. Replacing His23 in the latter mutant by Ala leads to a decrease in half-lifecompared to wild-type, but increase compared to the original mutant
additional information
mutant enzyme with C-terminal extension YQRYLADRLK to arrange a salt bridge between Arg140 and the proximal aspartate at position 10. Mutant shows an increase in Hill coefficient from 1.5 of wild-type to 1.99 and an increase in thermostability. Introduction of mutation D10N in this mutant reverts the Hill coefficient to 1.44
additional information
mutant protein carrying the C-terminal tail of Escherichia coli enzyme containing an Arg residue shows elevated cooperativity in the presence of phosphate
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