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4-deoxy-4-fluoro-D-glucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
D-glucarate
5-dehydro-4-deoxy-D-glucarate + H2O
D-idarate
L-glucarate
-
-
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
4-deoxy-4-fluoro-D-glucarate
?
-
-
?
4-deoxy-4-fluoro-D-glucarate
?
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
ir
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
Paracolobactrum sp.
-
-
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
-
-
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
3-deoxy-L-threo-2-hexulosarate + H2O
-
-
i.e. 4-deoxy-5-ketoglucarate
?
D-glucarate
5-dehydro-4-deoxy-D-glucarate + H2O
-
-
-
?
D-glucarate
5-dehydro-4-deoxy-D-glucarate + H2O
-
-
-
-
?
D-Glucarate
?
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-Glucarate
?
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-Glucarate
?
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-Glucarate
?
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-Glucarate
?
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-Glucarate
?
Paracolobactrum sp.
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-Glucarate
?
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-Glucarate
?
-
first step in D-glucarate metabolism in bacteria
-
-
?
D-glucarate
D-idarate
-
epimerization
epimerization
r
D-glucarate
D-idarate
-
epimerization
epimerization
r
D-glucarate
D-idarate
-
epimerization
epimerization
r
D-idarate
D-glucarate
-
epimerization
epimerization
r
D-idarate
D-glucarate
-
epimerization
epimerization
r
D-idarate
D-glucarate
-
epimerization
epimerization
r
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
i.e. 4-deoxy-5-ketoglucarate
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
i.e. 4-deoxy-5-ketoglucarate
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
i.e. 4-deoxy-5-ketoglucarate
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
-
-
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
i.e. 4-deoxy-5-ketoglucarate
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
i.e. 4-deoxy-5-ketoglucarate
?
L-idarate
3-deoxy-L-threo-2-hexulosarate
-
-
i.e. 4-deoxy-5-ketoglucarate
?
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0.28 - 44
4-deoxy-4-fluoro-D-glucarate
0.28
4-deoxy-4-fluoro-D-glucarate
pH 7.5, 22°C, N341D mutant
2.7
4-deoxy-4-fluoro-D-glucarate
pH 7.5, 22°C, N341L mutant
44
4-deoxy-4-fluoro-D-glucarate
pH 7.5, 22°C, wild-type enzyme
0.00033
D-glucarate
H339N mutant, pH 7.5, 22°C
0.001
D-glucarate
K207R mutant, pH 7.5, 22°C
0.004
D-glucarate
pH 7.5, 22°C, N341L mutant
0.005
D-glucarate
K207Q mutant, pH 7.5, 22°C
0.016
D-glucarate
H339A mutant, pH 7.5, 22°C
0.02
D-glucarate
H339Q mutant, pH 7.5, 22°C
0.022
D-glucarate
pH 7.5, 22°C, N341D mutant
0.19
D-glucarate
Y150F mutant, pH 7.5, 22°C
1
D-glucarate
D366A mutant, pH 7.5, 22°C
1.8
D-glucarate
D366N mutant, pH 7.5, 22°C
35
D-glucarate
pH 7.5, 22°C, wild-type enzyme
35
D-glucarate
-
pH 7.5, 22°C, wild-type enzyme
0.00025
L-idarate
H339N mutant, pH 7.5, 22°C
0.0015
L-idarate
K207Q mutant, pH 7.5, 22°C
0.0015
L-idarate
K207R mutant, pH 7.5, 22°C
0.0051
L-idarate
H339A mutant, pH 7.5, 22°C
0.02
L-idarate
H339Q mutant, pH 7.5, 22°C
0.036
L-idarate
pH 7.5, 22°C, N341L mutant
0.08
L-idarate
Y150F mutant, pH 7.5, 22°C
0.099
L-idarate
pH 7.5, 22°C, N341D mutant
1.5
L-idarate
D366A mutant, pH 7.5, 22°C
8
L-idarate
D366N mutant, pH 7.5, 22°C
34
L-idarate
pH 7.5, 22°C, wild-type enzyme
35
L-idarate
pH 7.5, 22°C, wild-type enzyme
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Marsh, C.A.
An enzymatic determination of D-glucaric acid by conversion to pyruvate
Anal. Biochem.
145
266-272
1985
Escherichia coli
brenda
Blumenthal, H.J.; Lucuta, V.L.; Blumenthal, D.C.
Specific enzymatic assay for D-glucarate in human serum
Anal. Biochem.
185
286-293
1990
Pseudomonas sp., Pseudomonas sp. A
brenda
Hubbard, B.K.; Koch, M.; Palmer, D.R.J.; Babbitt, P.C.; Gerlt, J.A.
Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli
Biochemistry
37
14369-14375
1998
Bacillus subtilis, Escherichia coli, Pseudomonas putida
brenda
Jeffcoat, R.
Chemical modification and kinetic studies on glucarate hydro-lyase from a species of Pseudomonas A
Eur. J. Biochem.
25
515-523
1972
Pseudomonas sp., Pseudomonas sp. A
brenda
Blumenthal, H.J.
D-glucarate dehydratase
Methods Enzymol.
9
660-665
1966
Klebsiella aerogenes, Priestia megaterium, Bacillus subtilis, Citrobacter freundii, Escherichia coli, Pectobacterium carotovorum, Klebsiella pneumoniae, Paracolobactrum sp.
-
brenda
Jeffcoat, R.; Hassall, H.; Dagley, S.
The metabolism of D-glucarate by Pseudomonas acidovorans
Biochem. J.
115
969-976
1969
Klebsiella aerogenes, Delftia acidovorans
brenda
Palmer, D.R.J.; Wieczorek, S.J.; Hubbard, B.K.; Mrachko, G.T.; Gerit, J.A.
Importance of mechanistic imperatives in enzyme-catalyzed beta-elimination reactions: stereochemical consequences of the dehydration reaction catalyzed by D-galactonate dehydratase from Escherichia coli and D-glucarate dehydratase from Pseudomonas putida
J. Am. Chem. Soc.
199
9580-9581
1997
Pseudomonas putida
-
brenda
Palmer, D.R.J.; Gerit, J.A.
Evolution of enzymatic activities: multiple pathways for generating and partitioning a common enolic intermediate by glucarate dehydratase from Pseudomonas putida
J. Am. Chem. Soc.
118
10323-10342
1996
Pseudomonas putida
-
brenda
Palmer, D.R.J.; Hubbard, B.K.; Gerit, J.A.
Evolution of enzymatic activities in the enolase superfamily: partitioning of reactive intermediates by (D)-glucarate dehydratase from Pseudomonas putida
Biochemistry
37
14350-14357
1998
Pseudomonas putida
brenda
Gulick, A.M.; Palmer, D.R.J.; Babbitt, P.C.; Gerlt, J.A.; Rayment, I.
Evolution of enzymatic activities in the enolase superfamily: crystal structure of (D)-glucarate dehydratase from Pseudomonas putida
Biochemistry
37
14358-14368
1998
Pseudomonas putida (P42206), Pseudomonas putida
brenda
Gulick, A.M.; Hubbard, B.K.; Gerlt, J.A.; Rayment, I.
Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glucarate dehydratase from Escherichia coli
Biochemistry
39
4590-4602
2000
Escherichia coli (P0AES2), Escherichia coli
brenda
Gulick, A.M.; Hubbard, B.K.; Gerlt, J.A.; Rayment, I.
Evolution of enzymatic activities in the enolase superfamily: identification of the general acid catalyst in the active site of D-glucarate dehydratase from Escherichia coli
Biochemistry
40
10054-10062
2001
Escherichia coli (P0AES2), Escherichia coli
brenda
Aghaie, A.; Lechaplais, C.; Sirven, P.; Tricot, S.; Besnard-Gonnet, M.; Muselet, D.; de Berardinis, V.; Kreimeyer, A.; Gyapay, G.; Salanoubat, M.; Perret, A.
New Insights into the Alternative D-Glucarate Degradation Pathway
J. Biol. Chem.
283
15638-15646
2008
Acinetobacter baylyi (Q6FFQ2)
brenda
Tian, B.; Wallrapp, F.; Kalyanaraman, C.; Zhao, S.; Eriksson, L.A.; Jacobson, M.P.
Predicting enzyme-substrate specificity with QM/MM methods: a case study of the stereospecificity of D-glucarate dehydratase
Biochemistry
52
5511-5513
2013
Pseudomonas putida
brenda