Information on EC 3.5.4.26 - diaminohydroxyphosphoribosylaminopyrimidine deaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.4.26
-
RECOMMENDED NAME
GeneOntology No.
diaminohydroxyphosphoribosylaminopyrimidine deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amidines
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
flavin biosynthesis I (bacteria and plants)
-
-
Metabolic pathways
-
-
Riboflavin metabolism
-
-
flavin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine 2-aminohydrolase
The substrate is the product of EC 3.5.4.25 GTP cyclohydrolase II.
CAS REGISTRY NUMBER
COMMENTARY hide
68994-19-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
show the reaction diagram
2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + H2O
5-amino-6-(5-phospho-D-ribosylamino)uracil + NH3
show the reaction diagram
2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H2O
5-amino-6-(5-phosphoribosylamino)uracil + NH3
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42038
2 * 42038, calculated from amino acid sequence
42441
2 * 42441, MALDI-TOF mass spectrometry
58000
gel filtration
80000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.125 M sodium cacodylate pH 5, 30% (w/v) polyethylene glycol 8000
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-6°C, 50 mM Tris-HCl buffer, pH 8, at least one month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nickel-charged HisTrap column chromatography and Superdex 200 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
Show AA Sequence (682 entries)
Please use the Sequence Search for a specific query.