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2,6-diaminopurine + H2O
?
2,6-diaminopurine + H2O
guanine + NH3
2-amino-6-bromopurine + H2O
?
2-amino-6-chloropurine + H2O
guanine + HCl
6-amino-2-hydroxypurine + H2O
xanthine + NH3
6-bromopurine + H2O
hypoxanthine + HBr
6-chloropurine + H2O
hypoxanthine + HCl
6-iodopurine + H2O
hypoxanthine + HI
6-[(E)-4-hydroxy-3-methylbut-2-enylamino]purine + H2O
?
1.8% of the activity of adenine
-
-
?
6-[(Z)-4-hydroxy-3-methylbut-2-enylamino]purine + H2O
?
1.7% of the activity of adenine
-
-
?
adenine + H2O
hypoxanthine + NH3
benzyladenine + H2O
hypoxanthine + 1-phenylmethaneamine
kinetin + H2O
hypoxanthine + 1-furan-2-ylmethaneamine
m-topolin + H2O
hypoxanthine + 2-(aminomethyl)phenol
N-6-methyladenine + H2O
?
N6-(2-isopentenyl)adenine + H2O
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
N6-isopentenyladenine + H2O
hypoxanthine + 3-methylbut-3-en-1-amine
additional information
?
-
2,6-diaminopurine + H2O
?
-
-
-
-
?
2,6-diaminopurine + H2O
?
73% activity compared to adenine
-
-
?
2,6-diaminopurine + H2O
?
73% activity compared to adenine
-
-
?
2,6-diaminopurine + H2O
?
-
-
-
-
?
2,6-diaminopurine + H2O
?
-
-
-
-
?
2,6-diaminopurine + H2O
?
-
-
-
-
?
2,6-diaminopurine + H2O
guanine + NH3
-
-
-
-
?
2,6-diaminopurine + H2O
guanine + NH3
-
-
-
-
?
2,6-diaminopurine + H2O
guanine + NH3
-
-
-
?
2,6-diaminopurine + H2O
guanine + NH3
-
-
-
?
2,6-diaminopurine + H2O
guanine + NH3
-
-
-
?
2,6-diaminopurine + H2O
guanine + NH3
-
-
-
?
2-amino-6-bromopurine + H2O
?
109% activity compared to adenine
-
-
?
2-amino-6-bromopurine + H2O
?
109% activity compared to adenine
-
-
?
2-amino-6-chloropurine + H2O
guanine + HCl
-
-
-
?
2-amino-6-chloropurine + H2O
guanine + HCl
-
-
-
?
6-amino-2-hydroxypurine + H2O
xanthine + NH3
-
-
-
?
6-amino-2-hydroxypurine + H2O
xanthine + NH3
-
-
-
?
6-bromopurine + H2O
hypoxanthine + HBr
-
-
-
?
6-bromopurine + H2O
hypoxanthine + HBr
-
-
-
?
6-bromopurine + H2O
hypoxanthine + HBr
-
-
-
?
6-bromopurine + H2O
hypoxanthine + HBr
-
-
-
?
6-chloropurine + H2O
?
119% activity compared to adenine
-
-
?
6-chloropurine + H2O
?
119% activity compared to adenine
-
-
?
6-chloropurine + H2O
?
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
-
-
-
-
?
6-iodopurine + H2O
hypoxanthine + HI
-
-
-
?
6-iodopurine + H2O
hypoxanthine + HI
-
-
-
?
6-iodopurine + H2O
hypoxanthine + HI
-
-
-
?
6-iodopurine + H2O
hypoxanthine + HI
-
-
-
?
6-methoxypurine + H2O
?
-
-
-
-
?
6-methoxypurine + H2O
?
-
-
-
-
?
6-methoxypurine + H2O
?
-
-
-
-
?
6-methoxypurine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
anaerobic conversion
-
-
?
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
ir
adenine + H2O
hypoxanthine + NH3
100% activity
-
-
?
adenine + H2O
hypoxanthine + NH3
100% activity
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
specific substrate
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
preferred substrate
-
-
?
adenine + H2O
hypoxanthine + NH3
-
preferred substrate
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
ir
adenine + H2O
hypoxanthine + NH3
-
-
-
ir
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
ir
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
?
benzyladenine + H2O
hypoxanthine + 1-phenylmethaneamine
0.7% of the activity of adenine
-
-
ir
benzyladenine + H2O
hypoxanthine + 1-phenylmethaneamine
3.8% of the activity of adenine
-
-
ir
FAD + H2O
?
-
-
-
-
?
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
-
-
?
kinetin + H2O
?
hydrolysis of adenine compounds, cleavage of cytokinins into inosine and the corresponding side chain amine
-
-
?
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
-
-
?
kinetin + H2O
?
hydrolysis of adenine compounds, cleavage of cytokinins into inosine and the corresponding side chain amine
-
-
?
kinetin + H2O
hypoxanthine + 1-furan-2-ylmethaneamine
2.6% of the activity of adenine
-
-
ir
kinetin + H2O
hypoxanthine + 1-furan-2-ylmethaneamine
2.8% of the activity of adenine
-
-
ir
m-topolin + H2O
hypoxanthine + 2-(aminomethyl)phenol
2.5% of the activity of adenine
-
-
ir
m-topolin + H2O
hypoxanthine + 2-(aminomethyl)phenol
4.0% of the activity of adenine
-
-
ir
N-6-methyladenine + H2O
?
-
-
-
-
?
N-6-methyladenine + H2O
?
-
-
-
-
?
N-6-methyladenine + H2O
?
-
-
-
-
?
N-6-methyladenine + H2O
?
-
-
-
-
?
N6-(2-isopentenyl)adenine + H2O
?
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
N6-(2-isopentenyl)adenine + H2O
?
hydrolysis of adenine compounds, low activity towards N6-substituted adenines, partial conversion, release of N6-side chain amines
-
-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
hydrolysis of adenine compounds, low activity towards N6-substituted adenines, partial conversion, release of N6-side chain amines
-
-
?
N6-isopentenyladenine + H2O
hypoxanthine + 3-methylbut-3-en-1-amine
2.1% of the activity of adenine
-
-
ir
N6-isopentenyladenine + H2O
hypoxanthine + 3-methylbut-3-en-1-amine
2.9% of the activity of adenine
-
-
ir
additional information
?
-
no substrate: adenosine
-
-
?
additional information
?
-
-
no substrate: adenosine
-
-
?
additional information
?
-
no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP
-
-
?
additional information
?
-
-
no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP
-
-
?
additional information
?
-
no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP
-
-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
-
-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
-
-
?
additional information
?
-
-
the protein exhibits partial amino acid sequence similarity to the CHASE domain of the cytokinin receptor, which may account for its ability to bind and hydrolyse not only kinetin, but also other cytokinins and other cytokinin derivatives, albeit at lower rates than its natural substrate adenine
-
-
?
additional information
?
-
the protein exhibits partial amino acid sequence similarity to the CHASE domain of the cytokinin receptor, which may account for its ability to bind and hydrolyse not only kinetin, but also other cytokinins and other cytokinin derivatives, albeit at lower rates than its natural substrate adenine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6-chloropurine + H2O
?
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
N6-(2-isopentenyl)adenine + H2O
?
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
additional information
?
-
adenine + H2O
?
-
anaerobic conversion
-
-
?
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
?
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
100% activity
-
-
?
adenine + H2O
hypoxanthine + NH3
100% activity
-
-
?
adenine + H2O
hypoxanthine + NH3
-
-
-
ir
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
adenine + H2O
hypoxanthine + NH3
-
-
-
ir
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
-
-
?
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
-
-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
-
-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.07 - 1.1
2,6-diaminopurine
1
2-amino-6-chloropurine
-
-
1.5
2-hydroxy-6-aminopurine
-
-
0.13 - 0.27
6-methoxypurine
0.088 - 0.09
N-6-methyladenine
0.07
2,6-diaminopurine
-
pH and temperature not specified in the publication
0.14
2,6-diaminopurine
-
pH and temperature not specified in the publication
0.29
2,6-diaminopurine
-
pH and temperature not specified in the publication
0.47
2,6-diaminopurine
at pH 7.4 and 40°C
1.1
2,6-diaminopurine
-
-
0.026
6-Chloropurine
-
pH and temperature not specified in the publication
0.056
6-Chloropurine
-
pH and temperature not specified in the publication
0.26
6-Chloropurine
-
pH and temperature not specified in the publication
0.13
6-methoxypurine
-
pH and temperature not specified in the publication
0.14
6-methoxypurine
-
pH and temperature not specified in the publication
0.16
6-methoxypurine
-
pH and temperature not specified in the publication
0.27
6-methoxypurine
-
pH and temperature not specified in the publication
0.0154
adenine
-
pH 7.0, 25°C
0.025
adenine
-
pH and temperature not specified in the publication
0.032
adenine
0.2 M phosphate buffer, 33°C
0.05
adenine
-
whole cells and cell extracts
0.055
adenine
0.2 M phosphate buffer, 33°C
0.17
adenine
-
pH and temperature not specified in the publication
0.2
adenine
-
pH and temperature not specified in the publication
0.2
adenine
-
pH and temperature not specified in the publication
0.23
adenine
-
pH 7.5, 30°C, wild-type Zn/Zn-ADE
0.29
adenine
pH 7.4, 35°C
0.3
adenine
-
pH 7.5, 30°C, wild-type Mn/Mn-ADE
0.31
adenine
-
mutant D474N, pH 7.5, 30°C
0.32
adenine
-
mutant D285A, pH 7.5, 30°C
0.32
adenine
-
mutant H214Q, pH 7.5, 30°C
0.32
adenine
-
mutant H473N, pH 7.5, 30°C
0.33
adenine
-
pH 7.5, 30°C, wild-type Fe/Fe-ADE
0.35
adenine
-
mutant D118N, pH 7.5, 30°C
0.35
adenine
-
mutant H90N, pH 7.5, 30°C
0.37
adenine
-
mutant H214N, pH 7.5, 30°C
0.38
adenine
-
mutant H92D, pH 7.5, 30°C
0.4
adenine
-
mutant H90Q, pH 7.5, 30°C
0.41
adenine
-
mutant E236Q, pH 7.5, 30°C
0.42
adenine
-
mutant H92N, pH 7.5, 30°C
0.44
adenine
-
mutant E121Q, pH 7.5, 30°C
0.44
adenine
-
mutant H90D, pH 7.5, 30°C
0.45
adenine
-
mutant H92C, pH 7.5, 30°C
0.47
adenine
-
mutant H120N, pH 7.5, 30°C
0.47
adenine
-
mutant S95A, pH 7.5, 30°C
0.48
adenine
-
mutant H235D, pH 7.5, 30°C
0.48
adenine
-
mutant H92Q, pH 7.5, 30°C
0.51
adenine
-
mutant H90C, pH 7.5, 30°C
0.66
adenine
at pH 7.4 and 40°C
0.68
adenine
-
pH 6.0, 30°C
0.8
adenine
-
pH 7.5, 37°C
0.8
adenine
-
mutant H235Q, pH 7.5, 30°C
0.82
adenine
-
mutant H235N, pH 7.5, 30°C
0.86
adenine
-
mutant D284A, pH 7.5, 30°C
0.92
adenine
-
wild-type, pH 7.0, temperature not specified in the publication
1.2
adenine
-
mutant H235C, pH 7.5, 30°C
1.6
adenine
-
mutant E185Q, pH 7.5, 30°C
2.4
adenine
-
mutant C127A, pH 7.0, temperature not specified in the publication
0.088
N-6-methyladenine
-
pH and temperature not specified in the publication
0.09
N-6-methyladenine
-
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.8 - 17
2,6-diaminopurine
0.42 - 1.9
6-Chloropurine
0.52 - 7.1
6-methoxypurine
0.48 - 1.28
N-6-methyladenine
1.8
2,6-diaminopurine
-
pH and temperature not specified in the publication
6.9
2,6-diaminopurine
-
pH and temperature not specified in the publication
12.3
2,6-diaminopurine
at pH 7.4 and 40°C
17
2,6-diaminopurine
-
pH and temperature not specified in the publication
0.42
6-Chloropurine
-
pH and temperature not specified in the publication
0.84
6-Chloropurine
-
pH and temperature not specified in the publication
1.9
6-Chloropurine
-
pH and temperature not specified in the publication
0.52
6-methoxypurine
-
pH and temperature not specified in the publication
1.9
6-methoxypurine
-
pH and temperature not specified in the publication
2.2
6-methoxypurine
-
pH and temperature not specified in the publication
7.1
6-methoxypurine
-
pH and temperature not specified in the publication
0.022
adenine
-
mutant D284A, pH 7.5, 30°C
0.036
adenine
-
mutant H90D, pH 7.5, 30°C
0.05
adenine
-
mutant H92N, pH 7.5, 30°C
0.053
adenine
-
mutant H90C, pH 7.5, 30°C
0.06
adenine
-
mutant E185Q, pH 7.5, 30°C
0.072
adenine
-
mutant E236Q, pH 7.5, 30°C
0.08
adenine
-
mutant H92Q, pH 7.5, 30°C
0.13
adenine
-
mutant H120N, pH 7.5, 30°C
0.5
adenine
-
mutant H214N, pH 7.5, 30°C
0.5
adenine
-
mutant H235N, pH 7.5, 30°C
0.69
adenine
-
mutant H92C, pH 7.5, 30°C
0.8
adenine
-
mutant H90Q, pH 7.5, 30°C
0.8
adenine
-
mutant H92D, pH 7.5, 30°C
1.2
adenine
-
mutant H235D, pH 7.5, 30°C
1.3
adenine
-
mutant H214Q, pH 7.5, 30°C
1.3
adenine
-
mutant H235Q, pH 7.5, 30°C
1.5
adenine
-
mutant H235C, pH 7.5, 30°C
2
adenine
-
wild-type, pH 7.5, 30°C
3 - 6
adenine
-
pH and temperature not specified in the publication
5.5
adenine
0.067 mM adenine in 0.2 M phosphate buffer, 33°C, pH 7.0
8
adenine
-
mutant H90N, pH 7.5, 30°C
8.1
adenine
-
pH 6.0, 30°C
16.75
adenine
-
pH 7.0, 25°C
20.8
adenine
at pH 7.4 and 40°C
22
adenine
-
mutant C127A, pH 7.0, temperature not specified in the publication
37
adenine
-
mutant D285A, pH 7.5, 30°C
56
adenine
-
pH and temperature not specified in the publication
57
adenine
-
mutant E121Q, pH 7.5, 30°C
61
adenine
-
pH and temperature not specified in the publication
62
adenine
-
pH and temperature not specified in the publication
78
adenine
-
mutant S95A, pH 7.5, 30°C
123
adenine
-
pH 7.5, 30°C, wild-type Zn/Zn-ADE
171
adenine
-
mutant D474N, pH 7.5, 30°C
173
adenine
-
mutant D118N, pH 7.5, 30°C
178
adenine
-
mutant H473N, pH 7.5, 30°C
185
adenine
-
pH 7.5, 30°C, wild-type Mn/Mn-ADE
196
adenine
-
pH 7.5, 30°C, wild-type Fe/Fe-ADE
200
adenine
-
wild-type, pH 7.5, 30°C, + Fe sequestered with a metal chelator, growth medium supplemented with Mn2+ prior to induction
221
adenine
-
wild-type, pH 7.0, temperature not specified in the publication
0.48
N-6-methyladenine
-
pH and temperature not specified in the publication
1.28
N-6-methyladenine
-
pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
19 - 120
2,6-diaminopurine
1.3 - 15
N-6-methyladenine
19
2,6-diaminopurine
-
pH and temperature not specified in the publication
24
2,6-diaminopurine
-
pH and temperature not specified in the publication
28
2,6-diaminopurine
-
pH and temperature not specified in the publication
120
2,6-diaminopurine
-
pH and temperature not specified in the publication
7.3
6-Chloropurine
-
pH and temperature not specified in the publication
9.6
6-Chloropurine
-
pH and temperature not specified in the publication
15
6-Chloropurine
-
pH and temperature not specified in the publication
16
6-Chloropurine
-
pH and temperature not specified in the publication
4
6-methoxypurine
-
pH and temperature not specified in the publication
12
6-methoxypurine
-
pH and temperature not specified in the publication
17
6-methoxypurine
-
pH and temperature not specified in the publication
26
6-methoxypurine
-
pH and temperature not specified in the publication
0.026
adenine
-
mutant D284A, pH 7.5, 30°C
0.038
adenine
-
mutant E185Q, pH 7.5, 30°C
0.082
adenine
-
mutant H90D, pH 7.5, 30°C
0.103
adenine
-
mutant H90C, pH 7.5, 30°C
0.119
adenine
-
mutant H92N, pH 7.5, 30°C
0.166
adenine
-
mutant H92Q, pH 7.5, 30°C
0.17
adenine
-
mutant E236Q, pH 7.5, 30°C
0.361
adenine
-
mutant H120N, pH 7.5, 30°C
0.609
adenine
-
mutant H235N, pH 7.5, 30°C
1.3
adenine
-
mutant H214N, pH 7.5, 30°C
1.3
adenine
-
mutant H235C, pH 7.5, 30°C
1.4
adenine
-
mutant H92C, pH 7.5, 30°C
1.6
adenine
-
mutant H235Q, pH 7.5, 30°C
2
adenine
-
mutant H90Q, pH 7.5, 30°C
2.1
adenine
-
mutant H92D, pH 7.5, 30°C
2.5
adenine
-
wild-type, pH 7.5, 30°C
2.5
adenine
-
mutant H235D, pH 7.5, 30°C
4
adenine
-
mutant H214Q, pH 7.5, 30°C
9
adenine
-
mutant C127A, pH 7.0, temperature not specified in the publication
11
adenine
-
pH 6.0, 30°C
22
adenine
-
mutant H90N, pH 7.5, 30°C
130
adenine
-
mutant D285A, pH 7.5, 30°C
130
adenine
-
mutant E121Q, pH 7.5, 30°C
160
adenine
-
mutant S95A, pH 7.5, 30°C
240
adenine
-
wild-type, pH 7.0, temperature not specified in the publication
310
adenine
-
pH and temperature not specified in the publication
330
adenine
-
pH and temperature not specified in the publication
500
adenine
-
wild-type, pH 7.5, 30°C, + Fe sequestered with a metal chelator, growth medium supplemented with Mn2+ prior to induction
540
adenine
-
pH 7.5, 30°C, wild-type Zn/Zn-ADE
550
adenine
-
mutant D474N, pH 7.5, 30°C
560
adenine
-
mutant D118N, pH 7.5, 30°C
560
adenine
-
mutant H473N, pH 7.5, 30°C
590
adenine
-
pH 7.5, 30°C, wild-type Fe/Fe-ADE
610
adenine
-
pH 7.5, 30°C, wild-type Mn/Mn-ADE
1400
adenine
-
pH and temperature not specified in the publication
3100
adenine
-
pH and temperature not specified in the publication
1.3
N-6-methyladenine
-
pH and temperature not specified in the publication
1.6
N-6-methyladenine
-
pH and temperature not specified in the publication
5.3
N-6-methyladenine
-
pH and temperature not specified in the publication
15
N-6-methyladenine
-
pH and temperature not specified in the publication
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D118N
-
mutant is able to bind 2 metals per active site, kcat (adenine): 173/sec
D284A
-
mutants is able to bind two equivalents of Mn2+ or Fe2+ in the active site but mutant is unable to catalyze the deaminase reaction
D285A
-
mutant is able to bind 2 metals per active site, kcat (adenine): 37/sec
D474N
-
mutant is able to bind 2 metals per active site, kcat (adenine): 171/sec
E121Q
-
mutant is able to bind 2 metals per active site, kcat (adenine): 57/sec
E185Q
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
E236Q
-
mutants is able to bind two equivalents of Mn2+ or Fe2+ in the active site but mutant is unable to catalyze the deaminase reaction
H120N
-
mutant is able to bind 2 metals per active site, kcat (adenine): 0.13/sec
H214N
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H214Q
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H235C
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H235D
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H235N
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H235Q
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H473N
-
mutant is able to bind 2 metals per active site, kcat (adenine): 178/sec
H90C
-
mutant is unable to bind either iron or manganese
H90D
-
mutant is unable to bind either iron or manganese
H90N
-
mutant is able to bind two equivalents of Mn2+ or Fe2+ per monomer and shows a kcat of about 5% of the wild-type enzyme
H90Q
-
mutant is unable to bind either iron or manganese
H92C
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H92D
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H92N
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
H92Q
-
mutant shows total loss of catalytic activity as well as the ability to bind divalent metals in the active site
S95A
-
mutant is able to bind 2 metals per active site, kcat (adenine): 78/sec
C127A
-
10fold decrease in kcat over the wild-type
C127S
-
complete loss of activity
C127A
-
10fold decrease in kcat over the wild-type
-
C127S
-
complete loss of activity
-
additional information
-
isolation and analysis of three deletion mutants srb10, srb11 and saf1 (ybr280c) affecting AAH1 expression during post-diauxic growth and in early stationary phase, overview
additional information
-
isolation and analysis of three deletion mutants srb10, srb11 and saf1 (ybr280c) affecting AAH1 expression during post-diauxic growth and in early stationary phase, overview
-
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-15°C, ammonium sulfate, for at least 2 months
-
4C, 10 mM phoshate buffer, pH7.5, 20 mM 2-mercaptoethanol, 1-2 weeks, minimal losses
4°C, 25 mM Tris, 100 mM NaCl, 15% glycerol and pH 6.0, stable for a month
-
activity of purified protein is stable for 1-2 weeks when stored at 4C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20C with 50% (v/v) glycerol and at -80C with 10-20% (v/v) glycerol or as freeze-dried powder
at 4C in potassium buffer above a concentration of 10 mg/ml and in presence of 20% glycerol stable for 1-2 weeks, prolonged storage in solution at -20C in presence of 50% glycerol and at -80C in presence of 10-20% glycerol, or as freeze-dried powder
4C, 10 mM phoshate buffer, pH7.5, 20 mM 2-mercaptoethanol, 1-2 weeks, minimal losses
-
4C, 10 mM phoshate buffer, pH7.5, 20 mM 2-mercaptoethanol, 1-2 weeks, minimal losses
-
activity of purified protein is stable for 1-2 weeks when stored at 4C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20C with 50% (v/v) glycerol and at -80C with 10-20% (v/v) glycerol or as freeze-dried powder
activity of purified protein is stable for 1-2 weeks when stored at 4C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20C with 50% (v/v) glycerol and at -80C with 10-20% (v/v) glycerol or as freeze-dried powder
at 4C in potassium buffer above a concentration of 10 mg/ml and in presence of 20% glycerol stable for 1-2 weeks, prolonged storage in solution at -20C in presence of 50% glycerol and at -80C in presence of 10-20% glycerol, or as freeze-dried powder
at 4C in potassium buffer above a concentration of 10 mg/ml and in presence of 20% glycerol stable for 1-2 weeks, prolonged storage in solution at -20C in presence of 50% glycerol and at -80C in presence of 10-20% glycerol, or as freeze-dried powder
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Jun, H.K.; Park, J.H.
Exogenous adenine deaminase production by Streptomyces sp. J-350P
Korean J. Appl. Microbiol. Bioeng.
12
31-36
1984
Streptomyces sp., Streptomyces sp. J-350P
-
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Discrete activities for adenine and adenosine deaminations in extracts of Streptomyces viridiviolaceus
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1981
Streptomyces viridiviolaceus
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Adenine aminohydrolase: occurrence and possible significance in trypanosomid flagellates
Proc. Natl. Acad. Sci. USA
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1979
Crithidia fasciculata, Leishmania braziliensis, Leishmania donovani, Leishmania mexicana, Leishmania tarentolae
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Adenine deaminase of a eukaryotic animal cell, Crithidia fasciculata
Arch. Biochem. Biophys.
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1977
Crithidia fasciculata
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Some properties of adenine deaminase of Pseudomonas synxantha
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57
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1979
Pseudomonas synxantha, Pseudomonas synxantha A3
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Purification and characterisation of adenine deaminase in Pseudomonas synxantha
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Purine, purine nucleoside, and purine nucleotide aminohydrolases
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4
47-78
1971
Cyberlindnera jadinii
-
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Adenine deaminase of Azobacter vinelandii
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79
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1957
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-
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Abbondandolo, A.; Weyer, A.; Heslot, H.; Lambert, M.
Study of adenine aminohydrolase in the yeast, Schizosaccharomyces pombe
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108
959-963
1971
Schizosaccharomyces pombe
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Adenine deaminase and adenine utilization in Saccharomyces cerevisiae
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1992
Saccharomyces cerevisiae
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Role of adenine deaminase in purine salvage and nitrogen metabolism and characterisation of the ade gene in Bacillus subtilis
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Adenine deaminase activity of the yicP gene product of Escherichia coli
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Escherichia coli
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Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases
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Catalytic mechanism and three-dimensional structure of adenine deaminase
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Escherichia coli
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Pa0148 from Pseudomonas aeruginosa catalyzes the deamination of adenine
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Paenarthrobacter aurescens, Burkholderia sp., Pseudomonas aeruginosa, Alteromonas macleodii
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Biochemical characterization of Kluyveromyces lactis adenine deaminase and guanine deaminase and their potential application in lowering purine content in beer
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Purine salvage in Methanocaldococcus jannaschii Elucidating the role of a conserved cysteine in adenine deaminase
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Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
brenda