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allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
allantoate + 2 H2O
ureidoglycolate + CO2 + 2 NH3
-
-
-
-
?
allantoate + 3 H2O
glyoxylate + 4 NH3 + 2 CO2
-
-
-
-
?
allantoate + H2O
(S)-ureidoglycine + NH3 + CO2
allantoate + H2O
ureidoglycine + NH3 + CO2
additional information
?
-
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
allantoate degradation is required to recycle purine-ring nitrogen in plants
-
-
?
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
an ureide
-
-
?
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
one of the crucial alternate steps in purine metabolism, allantoin catabolic pathway, overview
-
-
?
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
the enzyme is specific for allantoate, binding involves residues His228, Asn277, Arg290, and His384
-
-
?
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
A9GYV1
allantoate degradation is required to recycle purine-ring nitrogen in plants
-
-
?
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
A9GYV1
an ureide
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
Arthrobacter allantoicus
-
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
-
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
A9GYV1
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
A9GYV1
trancript accumulation of allantoate amidohydrolase not associated with an increased ureide catabolism observed in drought-tolerant genotypes, no close association with drought tolerance
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
-
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
-
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
-
allosteric regulation of the enzyme suggesting a critical role in the regulation of ureide degradation
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
Streptococcus allantoicus
-
-
-
-
?
allantoate + H2O
(S)-ureidoglycine + NH3 + CO2
-
-
-
?
allantoate + H2O
(S)-ureidoglycine + NH3 + CO2
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
?
additional information
?
-
substrate specificity, overview, no activity with N-alpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly
-
-
?
additional information
?
-
A9GYV1
substrate specificity, overview, no activity with N-lpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly
-
-
?
additional information
?
-
-
substrate specificity, overview, no activity with N-lpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly
-
-
?
additional information
?
-
-
no activity with ureidoglycolate
-
-
?
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allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
allantoate + 3 H2O
glyoxylate + 4 NH3 + 2 CO2
-
-
-
-
?
allantoate + H2O
(S)-ureidoglycine + NH3 + CO2
allantoate + H2O
ureidoglycine + NH3 + CO2
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
allantoate degradation is required to recycle purine-ring nitrogen in plants
-
-
?
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
one of the crucial alternate steps in purine metabolism, allantoin catabolic pathway, overview
-
-
?
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
A9GYV1
allantoate degradation is required to recycle purine-ring nitrogen in plants
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
Arthrobacter allantoicus
-
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
-
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
A9GYV1
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
-
-
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
-
allosteric regulation of the enzyme suggesting a critical role in the regulation of ureide degradation
-
-
?
allantoate + 2 H2O
ureidoglycolate + 2 NH3 + CO2
Streptococcus allantoicus
-
-
-
-
?
allantoate + H2O
(S)-ureidoglycine + NH3 + CO2
-
-
-
?
allantoate + H2O
(S)-ureidoglycine + NH3 + CO2
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
-
?
allantoate + H2O
ureidoglycine + NH3 + CO2
-
-
-
?
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Cd2+
-
can replace Mn2+, 85% of activity measured in the presence of Mn2+
Ni2+
-
can replace Mn2+, 32% of activity measured in the presence of Mn2+
sulfate
an allosteric effector responsible for stabilizing substrate binding
Zn2+
two co-catalytic zinc ions at the active site governs catalytic specificity for hydrolysis of N-carbamyl versus the peptide bond in exopeptidases, binding structure, the two zinc ions have subtly different coordination geometries, overview
additional information
-
Mg2+, Ca2+, Cu2+, Co2+ and Fe2+ can not replace Mn2+
Ca2+
Arthrobacter allantoicus
-
-
Ca2+
Streptococcus allantoicus
-
-
Co2+
Arthrobacter allantoicus
-
-
Co2+
Streptococcus allantoicus
-
-
Mn2+
-
Mn2+
Arthrobacter allantoicus
-
-
Mn2+
A9GYV1
required for activity
Mn2+
-
Mn2+ is essential for activity
Mn2+
A9GYV1
required for enzyme activity
Mn2+
-
essential for enzyme activity, catalyzes the second step of the developed assay, ammonia removal reaction in the presence of
Mn2+
-
no enzyme activity without Mn2+, maximal activity at concentrations between 0.1 and 0.5 mM
Mn2+
-
essential for enzyme activity, maximal activity between 0.1-0.5 mM
Mn2+
Streptococcus allantoicus
-
-
Mn2+
Streptococcus allantoicus
-
Mn2+ is essential for activity
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2-mercaptoethanol
-
21% inhibition at 1 mM
Cd2+
Streptococcus allantoicus
-
at pH 6.5
Fe2+
-
0.15 mM completely inhibits incubation mixture with Mn2+
glycolate
-
50% activity loss with 0.06 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.06 mM
glyoxylate
-
50% activity loss with 0.02 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.02 mM
guanidine
-
10% and 80% activity loss with 2 M and 5 M L-guanidine pre-treatment for 10 min at 30°C
hydroxylamine
-
67% inhibition at 1 mM
iminourea
-
pre-treatment with 2mM or 5mM results in a loss of acitivity of 10% or 80% of activity
N-Acetylimidazole
-
5.6% activity loss with 10 mM n-acetylimidazole 10 min at 30°C
N-bromosuccinimide
-
100% activity loss with 10 mM n-bromosuccinimide for 10 min at 30°C; pre-treatment completely inhibits activity
NEM
-
15% inhibition at 5 mM
Ni2+
Streptococcus allantoicus
-
at pH 6.5
nitrate
-
inhibits the enzyme, overview
pyridoxal 5'-phosphate
-
pre-treatment with 10 mM completely inhibits activity
pyridoxal-5-phosphate
-
100% activity loss with 10 mM pyridoxal-5-phosphate for 10 min at 30°C
SDS
-
50% activity loss with 50 mM SDS pre-treatment for 10 min at 30°C; pre-treatment with 50 mM results in a loss of acitivity of 50% of activity
Urea
-
5% and 20% activity loss with 2 M and 5 M urea pre-treatment for 10 min at 30°C
Allantoate
Arthrobacter allantoicus
-
substrate inhibition
Allantoate
Streptococcus allantoicus
-
substrate inhibition
Borate
-
Borate
-
8% inhibition at 10 mM
Co2+
-
0.15 mM completely inhibits incubation mixture with Mn2+
Co2+
Streptococcus allantoicus
-
at pH 6.5
Cu2+
Arthrobacter allantoicus
-
-
Cu2+
-
0.15 mM completely inhibits incubation mixture with Mn2+
Cu2+
Streptococcus allantoicus
-
-
EDTA
-
-
EDTA
-
abolishes activation of enzyme by Mn2+; final concentration of 2 mM
EDTA
-
complete inhibition at 10 mM, 85% inhibition at 5 mM, the activity is partially restored only by Mn2+, activity inhibited by EDTA is not recovered after EDTA removal by dialysis
fluoride
-
L-Asn
inhibition via chelating Mn2+, allantoate protects and reverses, competitive or mixed-type inhibition
L-Asn
A9GYV1
inhibition via chelating Mn2+, allantoate protects and reverses, competitive or mixed-type inhibition
L-Asp
inhibition via chelating Mn2+, allantoate protects and reverses
L-Asp
A9GYV1
inhibition via chelating Mn2+, allantoate protects and reverses
L-Asp
-
29% inhibition at 10 mM
Mn2+
Streptococcus allantoicus
-
-
Mn2+
Streptococcus allantoicus
-
at pH 6.5
Zn2+
Arthrobacter allantoicus
-
-
Zn2+
Streptococcus allantoicus
-
-
additional information
no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate
-
additional information
A9GYV1
no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate
-
additional information
-
no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate
-
additional information
-
pre-treatment with N-acetylimidazole, iodoacetic acid or 5,5'-dithiobis-(2-nitrobenzoic acid) has no effect on enzyme activity
-
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Vogels, G.D.
Reversible activation of allantoate amidohydrolase by acid-pretreatment and other properties of the enzyme
Biochim. Biophys. Acta
113
277-291
1966
Arthrobacter allantoicus, Streptococcus allantoicus
brenda
Van der Drift, C.; Vogels, G.D.
Activation and inactivation of allantoate amidohydrolase
Biochim. Biophys. Acta
139
162-168
1967
Streptococcus allantoicus
brenda
Van der Drift, C.; Vogels, G.D.
Allantoate amidohydrolase II. inactivation and instability
Enzymologia
36
278-286
1969
Streptococcus allantoicus
brenda
Van der Drift, C.; de Windt, F.E.; Vogels, G.D.
Allantoate hydrolysis by allantoate amidohydrolase
Arch. Biochem. Biophys.
136
273-279
1970
Streptococcus allantoicus
brenda
Bongaerts, G.P.A.; Vogels, G.D.
Uric acid degradation by Bacillus fastidiosus strains
J. Bacteriol.
125
689-697
1976
Metabacillus fastidiosus
brenda
Winkler, R.G.; Polacco, J.C.; Blevins, D.G.; Randall, D.D.
Enzymic degradation of allantoate in developing soybeans
Plant Physiol.
79
787-793
1985
Glycine max
brenda
Vitoria, A.P.; Mazzafera, P.
Xanthine degradation and related enzyme activities in leaves and fruits of two Coffea species differing in caffeine catabolism
J. Agric. Food Chem.
47
1851-1855
1999
Glycine max
brenda
Xu, Z.; de Windt, F.E.; van der Drift, C.
Purification and characterization of allantoate amidohydrolase from Bacillus fastidiosus
Arch. Biochem. Biophys.
324
99-104
1995
Metabacillus fastidiosus
brenda
Cusa, E.; Obradors, N.; Baldom, L.; Badia, J.; Aguilar, J.
Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli
J. Bacteriol.
181
7497-7484
1999
Escherichia coli
-
brenda
Xu, Z.W.; Zhou, H.X.; Huang, W.N.
Some properties of the allantoate amidohydrolase from French bean seedlings
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
30
460-468
2004
Phaseolus vulgaris
brenda
Todd, C.D.; Polacco, J.C.
AtAAH encodes a protein with allantoate amidohydrolase activity from Arabidopsis thaliana
Planta
223
1108-1113
2006
Arabidopsis thaliana
brenda
Muratsubaki, H.; Enomoto, K.; Soejima, A.; Satake, K.
An enzyme cycling method for measurement of allantoin in human serum
Anal. Biochem.
378
65-70
2008
Metabacillus fastidiosus, Metabacillus fastidiosus ATCC 29604
brenda
Agarwal, R.; Burley, S.K.; Swaminathan, S.
Structural analysis of a ternary complex of allantoate amidohydrolase from Escherichia coli reveals its mechanics
J. Mol. Biol.
368
450-463
2007
Escherichia coli K-12 (P77425)
brenda
Werner, A.K.; Sparkes, I.A.; Romeis, T.; Witte, C.P.
Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybean
Plant Physiol.
146
418-430
2008
Glycine max (A9GYV1), Glycine max, Arabidopsis thaliana (O49434)
brenda
Raso, M.J.; Munoz, A.; Pineda, M.; Piedras, P.
Biochemical characterization of an allantoate-degrading enzyme from French bean (Phaseolus vulgaris): the requirement of phenylhydrazine
Planta
226
1333-1342
2007
Phaseolus vulgaris
brenda
Charlson, D.V.; Korth, K.L.; Purcell, L.C.
Allantoate amidohydrolase transcript expression is independent of drought tolerance in soybean
J. Exp. Bot.
60
847-851
2009
Glycine max (A9GYV1)
brenda
Alamillo, J.M.; Diaz-Leal, J.L.; Sanchez-Moran, M.V.; Pineda, M.
Molecular analysis of ureide accumulation under drought stress in Phaseolus vulgaris L.
Plant Cell Environ.
33
1828-1837
2010
Phaseolus vulgaris (A6YS26)
brenda
Serventi, F.; Ramazzina, I.; Lamberto, I.; Puggioni, V.; Gatti, R.; Percudani, R.
Chemical basis of nitrogen recovery through the ureide pathway: Formation and hydrolysis of S-ureidoglycine in plants and bacteria
ACS Chem. Biol.
5
203-214
2010
Escherichia coli (P77425)
brenda
Diaz-Leal, J.L.; Torralbo, F.; Antonio Quiles, F.; Pineda, M.; Alamillo, J.M.
Molecular and functional characterization of allantoate amidohydrolase from Phaseolus vulgaris
Physiol. Plant.
152
43-58
2014
Phaseolus vulgaris (A6YS26)
brenda
Takagi, H.; Watanabe, S.; Tanaka, S.; Matsuura, T.; Mori, I.C.; Hirayama, T.; Shimada, H.; Sakamoto, A.
Disruption of ureide degradation affects plant growth and development during and after transition from vegetative to reproductive stages
BMC Plant Biol.
18
287
2018
Arabidopsis thaliana (O49434)
brenda