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(R)-2,2-dimethylcyclopropane carboxamide + H2O
(R)-2,2-dimethylcyclopropane carboxylic acid + NH3
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(R)-2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid
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amidase exhibits strict R-selectivity towards 2,2-dimethylcyclopropanecarboxamide
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(R)-piperazine-2-carboxamide + H2O
(R)-piperazine-2-carboxylic acid + NH3
(R)-piperazine-2-tert-butylcarboxamide + H2O
(R)-piperazine-2-carboxylate + tert-butylamine
(R)-piperidine-3-carboxamide + H2O
(R)-piperidine-3-carboxylic acid + NH3
beta-alaninamide + H2O
beta-alanine + NH3
D-glutamine amide + H2O
D-glutamic acid + NH3
no formation of glutamine, 27% of the activity with (R)-piperazine-2-carboxamide
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L-glutamine amide + H2O
L-glutamic acid + NH3
no formation of glutamine, 0.35% of the activity with (R)-piperazine-2-carboxamide
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piperidine-4-carboxamide + H2O
piperidine-4-carboxylic acid + NH3
0.23% of the activity with (R)-piperazine-2-carboxamide
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additional information
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(R)-piperazine-2-carboxamide + H2O
(R)-piperazine-2-carboxylic acid + NH3
hydrolysis with strict R-stereoselectivity
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(R)-piperazine-2-carboxamide + H2O
(R)-piperazine-2-carboxylic acid + NH3
hydrolysis with strict R-stereoselectivity
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(R)-piperazine-2-tert-butylcarboxamide + H2O
(R)-piperazine-2-carboxylate + tert-butylamine
hydrolysis with strict R-stereoselectivity, 9% of the activity with (R)-piperazine-2-carboxamide
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(R)-piperazine-2-tert-butylcarboxamide + H2O
(R)-piperazine-2-carboxylate + tert-butylamine
hydrolysis with strict R-stereoselectivity, 9% of the activity with (R)-piperazine-2-carboxamide
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(R)-piperidine-3-carboxamide + H2O
(R)-piperidine-3-carboxylic acid + NH3
68.9% of the activity with (R)-piperazine-2-carboxamide
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(R)-piperidine-3-carboxamide + H2O
(R)-piperidine-3-carboxylic acid + NH3
68.9% of the activity with (R)-piperazine-2-carboxamide
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beta-alaninamide + H2O
beta-alanine + NH3
108% of the activity with (R)-piperazine-2-carboxamide
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beta-alaninamide + H2O
beta-alanine + NH3
108% of the activity with (R)-piperazine-2-carboxamide
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additional information
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RamA has hydrolyzing activity toward the carboxamide compounds, in which an amino or imino group is connected to beta- or gamma-carbon, such as beta-alanine amide, (R)-piperazine-2-carboxamide (R)-piperidine-3-carboxamide, D-glutamine amide and (R)-piperazine-2-tert-butylcarboxamide. The enzyme does not act on the other amide substrates for the aliphatic amidase: D-alanine amide, D-valine amide, D-leucine amide, D-isoleucine amide, D-proline amide, D-phenylalanine amide, D-tryptophan amide, D-methionine amide, D-serine amide, D-threoninamide, D-tyrosine amide, D-aspartic acid amide, D-glutamic acid amide, D-lysine amide, D-arginine amide, D-histidine amide, L-alanine amide, L-valine amide, L-leucine amide, L-isoleucine amide, L-proline amide, L-phenylalanine amide, L-tryptophanamide, L-methioninamide, L-serine amide, L-threonine amide, L-tyrosine amide, L-asparagine amide, L-aspartic acid amide, L-glutamic acid amide, L-lysine amide, L-arginine amide, L-histidine amide, glycine amide and (R,S)-piperidine-2-carboxamide. Carboxamides of the side chains in D-asparagine, D-glutamine, L-asparagine and L-glutamine are not hydrolyzed by the enzyme. The enzyme does not show peptidase activity toward beta-alanyl-L-alanine, beta-alanylglycine, beta-alanyl-L-histidine, glycylglycine, glycylglycylglycine, L-alanylglycine, D-alanylglycine, D-alanylglycylglycine, DL-alanyl-DL-asparagine, DL-alanyl-DL-isoleucine, DL-alanyl-DL-leucine, DL-alanyl-DL-methionine, DL-alanyl-DL-phenylalanine, DL-alanyl-DL-serine, DL-alanyl-DL-valine and L-aspartyl-D-alanine. The enzyme could not hydrolyze the following aliphatic amides, aromatic amides and nitriles: acetamide, propionamide, n-butyramide, isobutyramide, n-valeramide, n-capronamide, crotonamide, methacrylamide, cyclohexanecarboxamide, benzamide, o-aminobenzamide, m-aminobenzamide, p-aminobenzamide, p-toluamide, p-chlorobenzamide, p-nitrobenzamide, 2-picolinamide, nicotinamide, pyridine-4-carboxamide, pyrazinamide,2-thiophenecarboxamide, phenylacetamide, indole-3-acetamide, acetonitrile, propionitrile, 3-hydroxypropionitrile, n-capronitrile, methacrylonitrile, crotononitrile, glutaronitrile, 2,4-dicyanobut-1-ene, beta-phenylpropionitrile, cinnamonitrile, 2-cyanopiperidine, 2-cyanopiperazine, phenylacetonitrile, 4-methoxyphenylacetonitrile, alpha-methylbenzyl cyanide, 2-pyridineacetonitrile, 3-pyridineacetonitrile, thiophene-2-acetonitrile, beta-indoleacetonitrile, diphenylacetonitrile, 4-chlorobenzyl cyanide, benzonitrile, 4-chlorobenzonitrile, 4-nitrobenzonitrile, p-tolunitrile, anisonitrile, 2-cyanophenol, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine, pyrazinecarbonitrile, 3-cyanoindole, a-naphthonitrile, 2-thiophenecarbonitrile, terephthalonitrile and isophthalonitrile
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additional information
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RamA has hydrolyzing activity toward the carboxamide compounds, in which an amino or imino group is connected to beta- or gamma-carbon, such as beta-alanine amide, (R)-piperazine-2-carboxamide (R)-piperidine-3-carboxamide, D-glutamine amide and (R)-piperazine-2-tert-butylcarboxamide. The enzyme does not act on the other amide substrates for the aliphatic amidase: D-alanine amide, D-valine amide, D-leucine amide, D-isoleucine amide, D-proline amide, D-phenylalanine amide, D-tryptophan amide, D-methionine amide, D-serine amide, D-threoninamide, D-tyrosine amide, D-aspartic acid amide, D-glutamic acid amide, D-lysine amide, D-arginine amide, D-histidine amide, L-alanine amide, L-valine amide, L-leucine amide, L-isoleucine amide, L-proline amide, L-phenylalanine amide, L-tryptophanamide, L-methioninamide, L-serine amide, L-threonine amide, L-tyrosine amide, L-asparagine amide, L-aspartic acid amide, L-glutamic acid amide, L-lysine amide, L-arginine amide, L-histidine amide, glycine amide and (R,S)-piperidine-2-carboxamide. Carboxamides of the side chains in D-asparagine, D-glutamine, L-asparagine and L-glutamine are not hydrolyzed by the enzyme. The enzyme does not show peptidase activity toward beta-alanyl-L-alanine, beta-alanylglycine, beta-alanyl-L-histidine, glycylglycine, glycylglycylglycine, L-alanylglycine, D-alanylglycine, D-alanylglycylglycine, DL-alanyl-DL-asparagine, DL-alanyl-DL-isoleucine, DL-alanyl-DL-leucine, DL-alanyl-DL-methionine, DL-alanyl-DL-phenylalanine, DL-alanyl-DL-serine, DL-alanyl-DL-valine and L-aspartyl-D-alanine. The enzyme could not hydrolyze the following aliphatic amides, aromatic amides and nitriles: acetamide, propionamide, n-butyramide, isobutyramide, n-valeramide, n-capronamide, crotonamide, methacrylamide, cyclohexanecarboxamide, benzamide, o-aminobenzamide, m-aminobenzamide, p-aminobenzamide, p-toluamide, p-chlorobenzamide, p-nitrobenzamide, 2-picolinamide, nicotinamide, pyridine-4-carboxamide, pyrazinamide,2-thiophenecarboxamide, phenylacetamide, indole-3-acetamide, acetonitrile, propionitrile, 3-hydroxypropionitrile, n-capronitrile, methacrylonitrile, crotononitrile, glutaronitrile, 2,4-dicyanobut-1-ene, beta-phenylpropionitrile, cinnamonitrile, 2-cyanopiperidine, 2-cyanopiperazine, phenylacetonitrile, 4-methoxyphenylacetonitrile, alpha-methylbenzyl cyanide, 2-pyridineacetonitrile, 3-pyridineacetonitrile, thiophene-2-acetonitrile, beta-indoleacetonitrile, diphenylacetonitrile, 4-chlorobenzyl cyanide, benzonitrile, 4-chlorobenzonitrile, 4-nitrobenzonitrile, p-tolunitrile, anisonitrile, 2-cyanophenol, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine, pyrazinecarbonitrile, 3-cyanoindole, a-naphthonitrile, 2-thiophenecarbonitrile, terephthalonitrile and isophthalonitrile
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additional information
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RamA has hydrolyzing activity toward the carboxamide compounds, in which an amino or imino group is connected to beta- or gamma-carbon, such as beta-alanine amide, (R)-piperazine-2-carboxamide (R)-piperidine-3-carboxamide, D-glutamine amide and (R)-piperazine-2-tert-butylcarboxamide. The enzyme does not act on the other amide substrates for the aliphatic amidase: D-alanine amide, D-valine amide, D-leucine amide, D-isoleucine amide, D-proline amide, D-phenylalanine amide, D-tryptophan amide, D-methionine amide, D-serine amide, D-threoninamide, D-tyrosine amide, D-aspartic acid amide, D-glutamic acid amide, D-lysine amide, D-arginine amide, D-histidine amide, L-alanine amide, L-valine amide, L-leucine amide, L-isoleucine amide, L-proline amide, L-phenylalanine amide, L-tryptophanamide, L-methioninamide, L-serine amide, L-threonine amide, L-tyrosine amide, L-asparagine amide, L-aspartic acid amide, L-glutamic acid amide, L-lysine amide, L-arginine amide, L-histidine amide, glycine amide and (R,S)-piperidine-2-carboxamide. Carboxamides of the side chains in D-asparagine, D-glutamine, L-asparagine and L-glutamine are not hydrolyzed by the enzyme. The enzyme does not show peptidase activity toward beta-alanyl-L-alanine, beta-alanylglycine, beta-alanyl-L-histidine, glycylglycine, glycylglycylglycine, L-alanylglycine, D-alanylglycine, D-alanylglycylglycine, DL-alanyl-DL-asparagine, DL-alanyl-DL-isoleucine, DL-alanyl-DL-leucine, DL-alanyl-DL-methionine, DL-alanyl-DL-phenylalanine, DL-alanyl-DL-serine, DL-alanyl-DL-valine and L-aspartyl-D-alanine. The enzyme could not hydrolyze the following aliphatic amides, aromatic amides and nitriles: acetamide, propionamide, n-butyramide, isobutyramide, n-valeramide, n-capronamide, crotonamide, methacrylamide, cyclohexanecarboxamide, benzamide, o-aminobenzamide, m-aminobenzamide, p-aminobenzamide, p-toluamide, p-chlorobenzamide, p-nitrobenzamide, 2-picolinamide, nicotinamide, pyridine-4-carboxamide, pyrazinamide,2-thiophenecarboxamide, phenylacetamide, indole-3-acetamide, acetonitrile, propionitrile, 3-hydroxypropionitrile, n-capronitrile, methacrylonitrile, crotononitrile, glutaronitrile, 2,4-dicyanobut-1-ene, beta-phenylpropionitrile, cinnamonitrile, 2-cyanopiperidine, 2-cyanopiperazine, phenylacetonitrile, 4-methoxyphenylacetonitrile, alpha-methylbenzyl cyanide, 2-pyridineacetonitrile, 3-pyridineacetonitrile, thiophene-2-acetonitrile, beta-indoleacetonitrile, diphenylacetonitrile, 4-chlorobenzyl cyanide, benzonitrile, 4-chlorobenzonitrile, 4-nitrobenzonitrile, p-tolunitrile, anisonitrile, 2-cyanophenol, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine, pyrazinecarbonitrile, 3-cyanoindole, a-naphthonitrile, 2-thiophenecarbonitrile, terephthalonitrile and isophthalonitrile
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