Information on EC 3.5.1.10 - formyltetrahydrofolate deformylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.5.1.10
-
RECOMMENDED NAME
GeneOntology No.
formyltetrahydrofolate deformylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenation
-
-
hydrolysis
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
folate transformations I
-
-
Glyoxylate and dicarboxylate metabolism
-
-
One carbon pool by folate
-
-
purine nucleobases degradation I (anaerobic)
-
-
purine nucleobases degradation II (anaerobic)
-
-
sulfopterin metabolism
-
-
tetrahydrofolate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate amidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-08-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cow
-
-
Manually annotated by BRENDA team
strain U-96
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
no activity in Columba sp.
pigeon
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + H2O
formate + tetrahydrofolate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + H2O
formate + tetrahydrofolate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
-
required for dehydrogenase reaction
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007 - 0.0095
10-formyltetrahydrofolate
0.049
Formyltetrahydrofolate
2.0 mM methionine added
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Burkholderia mallei (strain ATCC 23344)
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31800
calculated from the sequence of the cloned gene
32000
6 * 32000, SDS-PAGE
190000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
6 * 32000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydrolase domain, amino-terminal domain of enzyme
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purU gene cloned into overexpression vector pT7-7, pT7-PU1 tranformed into Escherichia coli BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H100A
-
soluble, shows enzymic activty
H106A
-
insoluble, no enzymic actvity
H106E
-
insoluble, no enzymic actvity
H106K
-
insoluble, no enzymic actvity
H106N
-
insoluble, no enzymic actvity
H106Q
-
insoluble, no enzymic actvity
H106R
-
insoluble, no enzymic actvity
H113A
-
insoluble, shows enzymic activty
H125A
-
soluble, shows enzymic activty
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