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Limulus clotting factor B
Limulus clotting factor Bbar
lipopolysaccharide + H2O
?
binds a pathogen-associated LPS molecule to trigger a blood coagulation cascade
-
?
N-tert-Butoxycarbonyl-Asp(O-benzyl)-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
N-tert-Butoxycarbonyl-Leu-Gly-Arg 4-nitroanilide + H2O
?
-
-
-
-
?
N-tert-Butoxycarbonyl-Thr-Ser-Arg 4-nitroanilide + H2O
?
-
-
-
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O
?
pro-factor B + H2O
factor B + ?
-
activation
-
-
?
tert-butoxycarbonyl-Ala-Pro-Arg-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-Ala-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
tert-butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-Asp(O-benzyl)-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
tert-butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
tert-butoxycarbonyl-Gly-Ala-Arg-4-methylcoumarin 7-amide + H2O
?
tert-butoxycarbonyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
tert-butoxycarbonyl-Ile-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
Tert-Butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
Tert-Butoxycarbonyl-Lys-Gly-Arg 4-methylcoumaryl 7-amide + H2O
?
Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
Tert-Butoxycarbonyl-Ser(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
Tert-Butoxycarbonyl-Val-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
additional information
?
-
Limulus clotting factor B
Limulus clotting factor Bbar
-
active factor Cbar selectively cleaves the Arg103-/-Ser104 and Ile124-/-Ile125 bonds in proenzyme factor B to form factor Bbar, which then activates proclotting enzyme to clotting enzyme. The resulting clotting enzyme acts on coagulogen, causing the formation of an insoluble coagulin gel
-
-
?
Limulus clotting factor B
Limulus clotting factor Bbar
-
active factor Cbar selectively cleaves the Arg103-/-Ser104 and Ile124-/-Ile125 bonds in proenzyme factor B to form factor Bbar, which then activates proclotting enzyme to clotting enzyme. The resulting clotting enzyme acts on coagulogen, causing the formation of an insiluble coagulin gel
-
-
?
N-tert-Butoxycarbonyl-Asp(O-benzyl)-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
N-tert-Butoxycarbonyl-Asp(O-benzyl)-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O
?
-
-
-
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O
?
-
-
-
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Asp(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Gln-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Gly-Ala-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Gly-Ala-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Gly-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Ile-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-Ile-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Lys(benzyloxycarbonyl)-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Lys-Gly-Arg 4-methylcoumaryl 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Lys-Gly-Arg 4-methylcoumaryl 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Met-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Ser(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Ser(O-benzyl)-Ala-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Val-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Val-Gly-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
additional information
?
-
blood coagulation cascade, which combines the function of hemostasis with immune response gainst gram-negative bacteria, besides blood coagulation and removal of endotoxin CrFC may be involved in cell signaling and protein-protein interaction
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
alpha-thrombin like specificity towards synthetic tripeptide substrates
-
-
?
additional information
?
-
-
factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus)
-
-
?
additional information
?
-
-
factor C is essential for the endotoxin-mediated coagulation system
-
-
?
additional information
?
-
-
alpha-thrombin like specificity towards synthetic tripeptide substrates
-
-
?
additional information
?
-
-
factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus)
-
-
?
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Limulus clotting factor B
Limulus clotting factor Bbar
-
active factor Cbar selectively cleaves the Arg103-/-Ser104 and Ile124-/-Ile125 bonds in proenzyme factor B to form factor Bbar, which then activates proclotting enzyme to clotting enzyme. The resulting clotting enzyme acts on coagulogen, causing the formation of an insoluble coagulin gel
-
-
?
lipopolysaccharide + H2O
?
binds a pathogen-associated LPS molecule to trigger a blood coagulation cascade
-
?
pro-factor B + H2O
factor B + ?
-
activation
-
-
?
additional information
?
-
additional information
?
-
blood coagulation cascade, which combines the function of hemostasis with immune response gainst gram-negative bacteria, besides blood coagulation and removal of endotoxin CrFC may be involved in cell signaling and protein-protein interaction
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus)
-
-
?
additional information
?
-
-
factor C is essential for the endotoxin-mediated coagulation system
-
-
?
additional information
?
-
-
factor C is an initiator in the hemolymph coagulation system of horseshoe crab (Limulus polyphemus, Tachypleus tridentatus)
-
-
?
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lipopolysaccharide
-
lipopolysaccharide
-
Escherichia coli serotype O55:B5 lipopolysaccharide (LPS) is used with a potency of 10 EU/ng, structure analysis, reconstitution of lyophilized LPS into solution. Factor C is activated through lipopolysaccharide. The state of aggregation of LPS impacts factor C activation, particularly in the presence of select salts and non-ionic surfactants that are commonly incorporated into pharmaceutical formulations. Analysis of aggregation status of LPS in aqueous solution using angle-dependent static and dynamic light scattering with and without chelating salts and polysorbate surfactants, and its correlation with activation of factor C, overview. Modulating effects of salts and surfactants on activation of factor C is associated with changes in the LPS aggregation
lipopolysaccharide
-
factor C activation through lipopolysaccharide assay. Detection of various LPS structural species by rFC-based assay. Several bacterial species are used as sources for endotoxin/lipopolysaccharide, overview. Highest activity is obtained with Escherichia coli LPS O111:B4 and J5, lowest activity with LPS from Francisella tularensis or Salmonella lipid A. Induction of interleukin IL-6 by various LPS structural species
additional information
-
the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide
-
additional information
-
the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide
-
additional information
-
the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide
-
additional information
-
the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide
-
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metabolism
-
factor C is involved in the LPS reactive coagulation cascade system in the Limulus horseshoe crab that results in the formation of a coagulin gel
metabolism
-
the enzyme is part of the clotting cascade of Limulus polyphemus. Factor C, the first component of the cascade, is a serine protease that is activated by endotoxin binding. The cascade, initiated by lipopolysaccharide, culminates in the activation of the pro-clotting enzyme to its active form, the clotting enzyme, which in turn acts on coagulogen to convert it into the coagulin clot
physiological function
-
RNAi silencing results in a significant reduction in phagocytic activity of tick hemocytes against the Gram-negative bacteria Chryseobacterium indologenes and Escherichia coli, but not against the yeast, Candida albicans
physiological function
-
Factor C activates factor B in the clotting cascade in Limulus polyphemus
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CFC_TACTR
1019
0
112346
Swiss-Prot
Secretory Pathway (Reliability: 1)
LFC_CARRO
Limulus rotundicauda
1019
0
112430
Swiss-Prot
Secretory Pathway (Reliability: 1)
LFC_CARRO
1019
0
112430
Swiss-Prot
Secretory Pathway (Reliability: 1)
A0A086PN09_TOXGO
1245
0
134156
TrEMBL
Secretory Pathway (Reliability: 2)
B7Q438_IXOSC
1597
0
174063
TrEMBL
Secretory Pathway (Reliability: 1)
B7P0L6_IXOSC
171
0
17827
TrEMBL
other Location (Reliability: 2)
S7UHP0_TOXGG
Toxoplasma gondii (strain ATCC 50853 / GT1)
1245
0
134214
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086LQJ6_TOXGO
1245
0
134214
TrEMBL
Secretory Pathway (Reliability: 2)
V5B813_TOXGV
Toxoplasma gondii (strain ATCC 50861 / VEG)
1251
0
134532
TrEMBL
Secretory Pathway (Reliability: 2)
G0QJZ1_ICHMG
Ichthyophthirius multifiliis (strain G5)
569
0
62297
TrEMBL
other Location (Reliability: 2)
B7PIL9_IXOSC
207
0
22859
TrEMBL
other Location (Reliability: 4)
A0A139Y3I4_TOXGO
1245
0
134156
TrEMBL
Secretory Pathway (Reliability: 2)
E0VH96_PEDHC
1471
1
163039
TrEMBL
Secretory Pathway (Reliability: 1)
A0A086JC79_TOXGO
1245
0
134188
TrEMBL
Secretory Pathway (Reliability: 2)
S7W690_TOXGG
Toxoplasma gondii (strain ATCC 50853 / GT1)
1251
0
134518
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086K3B2_TOXGO
1245
0
134214
TrEMBL
Secretory Pathway (Reliability: 2)
B7PM40_IXOSC
493
0
55640
TrEMBL
other Location (Reliability: 1)
B7QCS5_IXOSC
1056
1
115328
TrEMBL
Secretory Pathway (Reliability: 5)
B7QJR3_IXOSC
609
0
66593
TrEMBL
other Location (Reliability: 1)
A0A2G8YE62_TOXGO
1251
0
134483
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086M9M3_TOXGO
1251
0
134554
TrEMBL
Secretory Pathway (Reliability: 2)
L0B198_THEEQ
1242
0
137099
TrEMBL
Secretory Pathway (Reliability: 2)
A0A425I5P2_TOXGO
1245
0
134214
TrEMBL
Secretory Pathway (Reliability: 2)
B7PKL2_IXOSC
793
1
86136
TrEMBL
other Location (Reliability: 2)
A0A086PVW8_TOXGO
1251
0
134430
TrEMBL
Secretory Pathway (Reliability: 2)
E0VZV6_PEDHC
1103
1
121209
TrEMBL
other Location (Reliability: 2)
A0A2G8Y4E3_TOXGO
1245
0
134173
TrEMBL
Secretory Pathway (Reliability: 2)
S8GMI2_TOXGM
Toxoplasma gondii (strain ATCC 50611 / Me49)
1251
0
134502
TrEMBL
Secretory Pathway (Reliability: 2)
B7P0L5_IXOSC
431
0
47489
TrEMBL
other Location (Reliability: 1)
V4ZDR3_TOXGV
Toxoplasma gondii (strain ATCC 50861 / VEG)
1245
0
134314
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086L7D1_TOXGO
1251
0
134557
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086KEV0_TOXGO
1245
0
134214
TrEMBL
Secretory Pathway (Reliability: 2)
A0A425HZ30_TOXGO
1251
0
134518
TrEMBL
Secretory Pathway (Reliability: 2)
E0W1Q1_PEDHC
633
0
70697
TrEMBL
other Location (Reliability: 3)
S8F259_TOXGM
Toxoplasma gondii (strain ATCC 50611 / Me49)
1245
0
134156
TrEMBL
Secretory Pathway (Reliability: 2)
A0A139XPU1_TOXGO
1251
0
134411
TrEMBL
Secretory Pathway (Reliability: 2)
G0QJ76_ICHMG
Ichthyophthirius multifiliis (strain G5)
3800
0
423669
TrEMBL
other Location (Reliability: 1)
A0A2T6IJH5_TOXGO
1245
0
134180
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086QMB6_TOXGO
1245
0
134180
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086JFL9_TOXGO
1251
0
134532
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086JS18_TOXGO
1251
0
134532
TrEMBL
Secretory Pathway (Reliability: 2)
G0R0E3_ICHMG
Ichthyophthirius multifiliis (strain G5)
787
0
85197
TrEMBL
other Location (Reliability: 2)
B0ELZ4_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
2331
1
264852
TrEMBL
other Location (Reliability: 2)
B7QG80_IXOSC
264
0
28496
TrEMBL
other Location (Reliability: 2)
B7QFY0_IXOSC
181
0
19930
TrEMBL
other Location (Reliability: 2)
B7PD52_IXOSC
814
0
88169
TrEMBL
other Location (Reliability: 2)
A0A2T6IX49_TOXGO
1251
0
134557
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086QCG7_TOXGO
1251
0
134486
TrEMBL
Secretory Pathway (Reliability: 2)
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Tokunaga, F.; Nakajima, H.; Iwanaga, S.
Further studies on lipopolysaccharide-sensitive serine protease zymogen (factor C): its isolation from Limulus polyphemus hemocytes and identification as an intracellular zymogen activated by alpha-chymotrypsin, not by trypsin
J. Biochem.
109
150-157
1991
Limulus polyphemus, Tachypleus tridentatus
brenda
Nakamura, T.; Morita, T.; Iwanaga, S.
Lipopolysaccharide-sensitive serine-protease zymogen (factor C) found in Limulus hemocytes. Isolation and characterization
Eur. J. Biochem.
154
511-521
1986
Limulus polyphemus, Tachypleus sp.
brenda
Muta, T.; Miyata, T.; Misumi, Y.; Tokunaga, F.; Nakamura, T.; Toh, Y.; Ikehara, Y.; Iwanaga, S.
Limulus factor C. An endotoxin-sensitive serine protease zymogen with a mosaic structure of complement-like, epidermal growth factor-like, and lectin-like domains
J. Biol. Chem.
266
6554-6561
1991
Limulus sp.
brenda
Wang, L.; Ho, B.; Ding, J.L.
Transciptional regulation of limulus factor C
J. Biol. Chem.
278
49428-49437
2003
Carcinoscorpius rotundicauda (Q26422)
brenda
Alwis, K.U.; Milton, D.K.
Recombinant factor C assay for measuring endotoxin in house dust: comparison with LAL, and (1->3)-beta-D-glucans
Am. J. Ind. Med.
49
296-300
2006
Limulus sp.
brenda
Kawabata, S.; Muta, T.; Iwanaga, S.
Limulus coagulation factor C
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2nd. ed.
1740-1742
2004
Tachypleus sp.
-
brenda
Urbanova, V.; Hartmann, D.; Grunclova, L.; Sima, R.; Flemming, T.; Hajdusek, O.; Kopacek, P.
IrFC - an Ixodes ricinus injury-responsive molecule related to Limulus Factor C
Dev. Comp. Immunol.
46
439-447
2014
Ixodes ricinus
brenda
Wang, C.; Nelson, T.; Chen, D.; Ellis, J.C.; Abbott, N.L.
Understanding lipopolysaccharide aggregation and its influence on activation of Factor C
J. Colloid Interface Sci.
552
540-553
2019
Limulus polyphemus
brenda
Abate, W.; Sattar, A.A.; Liu, J.; Conway, M.E.; Jackson, S.K.
Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay
J. Med. Microbiol.
66
888-897
2017
Limulus polyphemus
brenda