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Ac-SQLRANISH-4-nitroanilide + H2O
?
-
weak chromogenic substrate
-
?
Ac-SQLRANISH-p-nitroanilide + H2O
?
-
-
-
-
?
complement component C3 + H2O
?
-
-
-
?
complement component C3a + H2O
?
-
-
-
?
Cys-Val-Val-Ala-Ser-Gln-Leu-Arg-Ala-Asn-Ile-His-Gln-Asp-Met-Gln-Leu-Gly-Arg
?
-
substrate is cleaved into 2 fragments
-
?
Cys-Val-Val-Ala-Ser-Gln-Leu-Arg-Ala-Asn-Ile-Ser-His-Lys-Asp-Met-Gln-Leu-Gly-Arg
?
-
-
-
?
glutathione S-transferase-chemotaxin C5a-GFP + H2O
?
-
fusion protein
-
?
N-terminal His-tagged C5a fusion protein + H2O
?
-
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of cleaved HT-C5a indicate a loss of 830 Da, consistent with the removal of seven residues from the C-terminus
-
-
?
rGST-C5a-enhanced green fluorescent protein + H2O
?
SQLRANISHKDMQLGR + H2O
?
-
good substrate
-
?
VVASQLRANISH + H2O
VVA + SQLRANISH
-
-
-
?
VVASQLRANISHKDMQLGR + H2O
VVASQLRANISH + KDMQLGR
-
-
-
?
additional information
?
-
chemotaxin C5a
?
-
-
-
?
chemotaxin C5a
?
-
cleavage site is His-Lys rather than Lys-Asp, native protein is resistant to cleavage, suggesting that His-Lys bond is inaccessible prior to proteolytic cleavage by C5 convertase
-
?
chemotaxin C5a + H2O
?
-
-
-
?
chemotaxin C5a + H2O
?
-
-
-
?
chemotaxin C5a + H2O
?
-
-
-
?
chemotaxin C5a + H2O
?
-
-
-
?
chemotaxin C5a + H2O
?
-
-
-
-
?
chemotaxin C5a + H2O
?
-
cleaves between His67 and Lys68 or Ala58 and Ser59
-
?
chemotaxin C5a + H2O
?
-
removes a six-amino-acid fragment and thereby inactivates this chemotaxin
-
?
chemotaxin C5a + H2O
?
-
-
-
-
?
chemotaxin C5a + H2O
?
-
-
-
-
?
chemotaxin C5a + H2O
?
-
cleavage between histidine-67 and lysine-68 near the C-terminus
-
-
?
rGST-C5a-enhanced green fluorescent protein + H2O
?
-
-
-
-
?
rGST-C5a-enhanced green fluorescent protein + H2O
?
-
-
-
-
?
additional information
?
-
-
no substrate: Cys-Val-Val-Ala-Ser-Gln-Leu-Arg-Ala-Asn-Ile-His-Pro-Lys-Asp-Met-Gln-Leu-Gly-Arg
-
?
additional information
?
-
-
alters the normal host inflammatory response by delaying the accumulation of PMNLs at the foci of streptococcal infection
-
?
additional information
?
-
-
binds directly to HEp2 and A549 cells, binds to the extracellular matrix protein fibronectin, role in cell invasion
-
?
additional information
?
-
-
mediates adherence to fibronectin, role in immune evasion by attenuating recruitment of polymorphonuclear leukocytes to the site of infection
-
?
additional information
?
-
-
no cleavage of rGST-enhanced green fluorescent protein
-
-
?
additional information
?
-
-
no cleavage of rGST-enhanced green fluorescent protein
-
-
?
additional information
?
-
-
native bovine serum albumin and other native proteins are highly resistant to proteolysis
-
-
?
additional information
?
-
-
fibronectin adhesin activity. Affinity for fibronectin is very high for both full-length recombinant ScpB and the 110-amino-acid phage display fragment
-
-
?
additional information
?
-
-
group B Streptococci do not bind to fibronectin in solution, but bind to fibronectin adsorbed onto a solid surface through ScpB, that binds to a motif created by the juxtaposition of multiple fibronectin molecules
-
-
?
additional information
?
-
-
fibronectin adhesin activity. Affinity for fibronectin is very high for both full-length recombinant ScpB and the 110-amino-acid phage display fragment
-
-
?
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COVID-19
Enzyme kinetic and binding studies identify determinants of specificity for the immunomodulatory enzyme ScpA, a C5a inactivating bacterial protease.
Diphtheria
Vaccination against Group B streptococcus.
Fasciitis, Necrotizing
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils.
Impetigo
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils.
Infant, Newborn, Diseases
Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35-37?weeks of gestation.
Infections
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils.
Infections
Efficacy of polymeric encapsulated C5a peptidase-based group B streptococcus vaccines in a murine model.
Infections
Enzyme kinetic and binding studies identify determinants of specificity for the immunomodulatory enzyme ScpA, a C5a inactivating bacterial protease.
Infections
Human serum induces streptococcal c5a peptidase expression.
Infections
Immune response to group A streptococcal C5a peptidase in children: implications for vaccine development.
Infections
Immunization with C5a peptidase from either group A or B streptococci enhances clearance of group A streptococci from intranasally infected mice.
Infections
Impact of M49, Mrp, Enn, and C5a peptidase proteins on colonization of the mouse oral mucosa by Streptococcus pyogenes.
Infections
Multifunctional glyceraldehyde-3-phosphate dehydrogenase of Streptococcus pyogenes is essential for evasion from neutrophils.
Infections
Protective immunization in mice against group B streptococci using encapsulated C5a peptidase.
Pharyngitis
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils.
Pharyngitis
An Experimental Group A Streptococcus Vaccine That Reduces Pharyngitis and Tonsillitis in a Nonhuman Primate Model.
Rheumatic Fever
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils.
Rheumatic Heart Disease
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils.
Shock, Septic
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils.
Streptococcal Infections
Human immunogenicity studies on group A streptococcal C5a peptidase (SCPA) as a potential vaccine against group A streptococcal infections.
Streptococcal Infections
In vivo Streptococcus pyogenes C5a peptidase activity: analysis using transposon- and nitrosoguanidine-induced mutants.
Tetanus
Vaccination against Group B streptococcus.
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D130A/S512A
-
SCP mutant with 949-residue fragment
D130A/S512A
-
no hydrolysis of substrate
L512A
-
mutant with reduced proteolytic activity
D130A
-
strain 90 226 SCPA-mutant, impaired capacity to infect nasal mucosa-associated lymphoid tissue
D130A
-
mutant with reduced proteolytic activity
D130A
residue is not critical for catalytic activity
H193A
complete loss of activity
H193A
-
mutant with reduced proteolytic activity
N295A
-
mutant with reduced proteolytic activity
N295A
residue is not critical for catalytic activity
S512A
-
no autocatalytic cleavage
S512A
residue is not critical for catalytic activity
additional information
-
analysis of M-like protein (simA) and C5a peptidase, two gene homologues of Streptococcus iniae, through allelic replacement reveals that M-like protein plays a significant role in Streptococcus iniae virulence and C5a peptidase does not
additional information
-
isogenic scpA-deficient mutant strain TR-9
additional information
-
isogenic scpA-deficient mutant strain TR-9
-
additional information
-
isogenic M1-mutant, expressses SCPA on cell surface
additional information
-
mutant ScpBdelta, a common natural variant of ScpB, contains a 4-amino-acid deletion that eliminates peptidase activity. High affinity for fibronectin is maintained in ScpBdelta. ScpB and scpBdelta can complement the fibronectin-binding defect of the deletion mutant strain TOH97, that does not express scpB. Thus, the delta12 allele has no effect on the fibronectin binding of recombinant ScpB, recombinant ScpB-phage display fragment, or ScpB expressed by group B streptococci. High-affinity interaction between ScpB and immobilized fibronectin is responsible for the maintenance of the scpB gene in strains lacking C5a peptidase activity
additional information
-
mutant ScpBdelta, a common natural variant of ScpB, contains a 4-amino-acid deletion that eliminates peptidase activity. High affinity for fibronectin is maintained in ScpBdelta. ScpB and scpBdelta can complement the fibronectin-binding defect of the deletion mutant strain TOH97, that does not express scpB. Thus, the delta12 allele has no effect on the fibronectin binding of recombinant ScpB, recombinant ScpB-phage display fragment, or ScpB expressed by group B streptococci. High-affinity interaction between ScpB and immobilized fibronectin is responsible for the maintenance of the scpB gene in strains lacking C5a peptidase activity
-
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medicine
-
acts as a potential invasin of group A streptococcus and promotes invasion independent of fibronectin
medicine
-
immunization with C5a peptidase protein from either group A or group B streptococci provides protection against group A streptococcus infections in mice, moreover, mice immunized with antibodies directed against protein from group B streptococci also cleared streptococci from their lungs more efficiently than those immunized with tetanus toxoid
medicine
-
inactivates recombinant chemotaxin C5a by destroying its capacity to interact with the C5a receptor on human polymorphonuclear leukocytes
medicine
-
intranasal immunization with SCPA prevents colonization and infection of human tonsils, thereby eliminating potential reservoirs that maintain endemic disease
medicine
-
SCPA is highly immunogenic in children infected with group A streptococcal pharyngitis
medicine
-
encapsulated C5a peptidase elicites significant immune responses and protection against group B streptococci challenge. C5a peptidase microsphere encapsulation has potential as a group B streptococci vaccine
medicine
-
fibronectin adhesin activity of scpB plays a role in virulence
medicine
-
increased anti-SCPA IgG levels in rheumatic fever, rheumatic heart disease and acute glomerulo nephritis cases. The rise of anti-SCPA IgG observed in patients is irrespective of different emm types of group A streptococci strains isolated from these patients. Indian females show higher concentration of anti-SCPA antibodies than male patients. Anti-SCPA IgG1 and anti-SCPA IgG3 are the predominant subclasses of anti-SCPA antibody and clearly distinguish individuals with acute rheumatic fever from those free of streptococcal disease
medicine
-
encapsulated C5a peptidase elicits significant immune responses and protection against a group B Streptococcus challenge. C5a peptidase poly(lactide-coglycolide) microsphere encapsulation has potential as a group B Streptococcus vaccine
medicine
-
the enzyme is used for vaccine development against group B Streptococcus
medicine
the enzyme can be useful for development of subunit vaccine against the pathogen Streptococcus zooepidemicus
medicine
inactive mutant H193A elicits high titers of antigen-specific IgG1 antibodies and robust T cell-mediated immunities in mice showing potential as a Streptococcus vaccine. Cconjugating the trisaccharide repeating unit of Streptococcus polysaccharide with mutant H193A converts the nonimmunogenic oligosaccharide into a highly active and T cell-dependent antigen
medicine
-
fibronectin adhesin activity of scpB plays a role in virulence
-
medicine
-
the enzyme can be useful for development of subunit vaccine against the pathogen Streptococcus zooepidemicus
-
additional information
-
greatly reduces ability to bind to receptors of polymorphonuclear leukocytes as compared with native C5a, inactivation is temperature dependent, mediates a small decrease in the molecular weight of C5adesArg and a six-residue peptide in inactivated C5a is lost
additional information
-
ScpA and the multifunctional protein streptococcal plasmin receptor/surface dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase are both necessary for the cleavage of C5a on the bacterial surface
additional information
-
streptococcal C5a peptidase binding to integrins by the Arg-Gly-Asp motifs may stabilize conformational changes required for substrate binding
additional information
-
ScpA and the multifunctional protein streptococcal plasmin receptor/surface dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase are both necessary for the cleavage of C5a on the bacterial surface
-
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Anderson, E.T.; Wetherell, M.G.; Winter, L.A.; Olmsted, S.B.; Cleary, P.P.; Matsuka, Y.V.
Processing, stability, and kinetic parameters of C5a peptidase from Streptococcus pyogenes
Eur. J. Biochem.
269
4839-4851
2002
Streptococcus pyogenes
brenda
Cleary, P.P.; Prahbu, U.; Dale, J.B.; Wexler, D.E.; Handley, J.
Streptococcal C5a peptidase is a highly specific endopeptidase
Infect. Immun.
60
5219-5223
1992
Streptococcus pyogenes
brenda
Cheng, Q.; Stafslien, D.; Purushothaman, S.S.; Cleary, P.
The group B streptococcal C5a peptidase is both a specific protease and an invasin
Infect. Immun.
70
2408-2413
2002
Streptococcus pyogenes
brenda
Beckmann, C.; Waggoner, J.D.; Harris, T.O.; Tamura, G.S.; Rubens, C.E.
Identification of novel adhesins from Group B streptococci by use of phage display reveals that C5a peptidase mediates fibronectin binding
Infect. Immun.
70
2869-2876
2002
Streptococcus pyogenes
brenda
Stafslien, D.K.; Cleary, P.P.
Characterization of the streptococcal C5a peptidase using a C5a-green fluorescent protein fusion protein substrate
J. Bacteriol.
182
3254-3258
2000
Streptococcus pyogenes
brenda
O'Connor, S.P.; Cleary, P.P.
In vivo Streptococcus pyogenes C5a peptidase activity: analysis using transposon- and nitrosoguanidine-induced mutants
J. Infect. Dis.
156
495-504
1987
Streptococcus pyogenes
brenda
Bohnsack, J.F.; Mollison, K.W.; Buko, A.M.; Ashworth, J.C.; Hill, H.R.
Group B streptococci inactivate complement component C5a by enzymic cleavage at the C-terminus
Biochem. J.
273
635-640
1991
Streptococcus sp.
-
brenda
Purushothaman, S.S.; Park, H.; Cleary, P.P.
Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus
Indian J. Med. Res.
119
44-47
2004
Streptococcus sp.
-
brenda
Shet, A.; Kaplan, E.; Johnson, D.; Cleary, P.P.
Human immunogenicity studies on group A streptococcal C5a peptidase (SCPA) as a potential vaccine against group A streptococcal infections
Indian J. Med. Res.
119 Suppl
95-98
2004
Streptococcus sp.
brenda
Park, H.S.; Cleary, P.P.
Active and passive intranasal immunizations with streptococcal surface protein C5a peptidase prevent infection of murine nasal mucosa-associated lymphoid tissue, a functional homologue of human tonsils
Infect. Immun.
73
7878-7886
2005
Streptococcus sp.
brenda
Terao, Y.; Yamaguchi, M.; Hamada, S.; Kawabata, S.
Multifunctional glyceraldehyde-3-phosphate dehydrogenase of Streptococcus pyogenes is essential for evasion from neutrophils
J. Biol. Chem.
281
14215-14223
2006
Streptococcus pyogenes, Streptococcus pyogenes SSI-9
brenda
Brown, C.K.; Gu, Z.Y.; Matsuka, Y.V.; Purushothaman, S.S.; Winter, L.A.; Cleary, P.P.; Olmsted, S.B.; Ohlendorf, D.H.; Earhart, C.A.
Structure of the streptococcal cell wall C5a peptidase
Proc. Natl. Acad. Sci. USA
102
18391-18396
2005
Streptococcus agalactiae
brenda
Wexler, D.E.; Chenoweth, D.E.; Cleary, P.P.
Mechanism of action of the group A streptococcal C5a inactivator
Proc. Natl. Acad. Sci. USA
82
8144-8148
1985
Streptococcus sp.
brenda
Cleary, P.P.; Matsuka, Y.V.; Huynh, T.; Lam, H.; Olmsted, S.B.
Immunization with C5a peptidase from either group A or B streptococci enhances clearance of group A streptococci from intranasally infected mice
Vaccine
22
4332-4341
2004
Streptococcus pyogenes
brenda
Hull, J.R.; Tamura, G.S.; Castner, D.G.
Interactions of the streptococcal C5a peptidase with human fibronectin
Acta Biomater.
4
504-513
2008
Streptococcus sp.
brenda
Santillan, D.A.; Andracki, M.E.; Hunter, S.K.
Protective immunization in mice against group B streptococci using encapsulated C5a peptidase
Am. J. Obstet. Gynecol.
198
114.e1-114.e6
2008
Streptococcus sp.
brenda
Tamura, G.S.; Hull, J.R.; Oberg, M.D.; Castner, D.G.
High-affinity interaction between fibronectin and the group B streptococcal C5a peptidase is unaffected by a naturally occurring four-amino-acid deletion that eliminates peptidase activity
Infect. Immun.
74
5739-5746
2006
Streptococcus sp., Streptococcus sp. COH1
brenda
Karmarkar, M.G.; Santosh, K.; Vineetha, V.; Mehta, P.R.; Kaplan, E.; Yurecho, R.; Cleary, P.P.
Human immune response to streptococcal C5a peptidase in Indian children and adults
Int. Congr. Ser.
1289
54-57
2006
Streptococcus sp.
-
brenda
Kagawa, T.F.; OConnell, M.R.; Mouat, P.; Paoli, M.; OToole, P.W.; Cooney, J.C.
Model for substrate interactions in C5a peptidase from Streptococcus pyogenes: A 1.9 A crystal structure of the active form of ScpA
J. Mol. Biol.
386
754-772
2009
Streptococcus pyogenes
brenda
Locke, J.B.; Aziz, R.K.; Vicknair, M.R.; Nizet, V.; Buchanan, J.T.
Streptococcus iniae M-like protein contributes to virulence in fish and is a target for live attenuated vaccine development
PLoS ONE
3
e2824
2008
Streptococcus iniae
brenda
Gleich-Theurer, U.; Aymanns, S.; Haas, G.; Mauerer, S.; Vogt, J.; Spellerberg, B.
Human serum induces streptococcal c5a peptidase expression
Infect. Immun.
77
3817-3825
2009
Streptococcus agalactiae, Streptococcus pyogenes
brenda
Santillan, D.A.; Rai, K.K.; Santillan, M.K.; Krishnamachari, Y.; Salem, A.K.; Hunter, S.K.
Efficacy of polymeric encapsulated C5a peptidase-based group B streptococcus vaccines in a murine model
Am. J. Obstet. Gynecol.
205
249e1-249e8
2011
Streptococcus sp.
brenda
Yue, L.; Zhou, Y.; Zhang, S.
Epitope prediction, fractional expression and immunogenicity of streptococcal C5a peptidase from group B Streptococcus
Chin. J. Biol.
23
460-465
2010
Streptococcus sp.
-
brenda
Wei, Z.; Fu, Q.; Chen, Y.; Li, M.; Cong, P.; Mo, D.; Liu, X.
Streptococcus equi ssp. zooepidemicus C5a peptidase, a putative invasin, induces protective immune response in mice
Res. Vet. Sci.
95
444-450
2013
Streptococcus equi subsp. zooepidemicus (B4U2T7), Streptococcus equi subsp. zooepidemicus MGCS10565 (B4U2T7)
brenda
Lynskey, N.N.; Reglinski, M.; Calay, D.; Siggins, M.K.; Mason, J.C.; Botto, M.; Sriskandan, S.
Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase
PLoS Pathog.
13
e1006493
2017
Streptococcus pyogenes serotype M1 (P58099)
brenda
Li, H.; Wang, S.; Zhao, Y.; Chen, Z.; Gu, G.; Guo, Z.
Mutagenesis and immunological evaluation of group A streptococcal C5a peptidase as an antigen for vaccine development and as a carrier protein for glycoconjugate vaccine design
RSC Adv.
7
42056-42063
2017
Streptococcus pyogenes (B6ETQ5)
-
brenda