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ADAM9 + H2O
?
-
54% cleavage
-
-
?
aggrecan + H2O
aggrecan fragments
-
the HtrA1-specific cleavage site is VQTV3562357TWPD within the interglobular domain of aggrecan
-
-
?
alpha-lactalbumin + H2O
?
alpha-tubulin + H2O
?
-
-
-
-
?
alpha2-macroglobulin + H2O
?
-
55% cleavage
-
-
?
amylase MalS + H2O
?
-
-
-
?
apolipoprotein-E + H2O
?
-
-
-
?
Arc repressor + H2O
?
-
-
-
?
autolysin AcmA + H2O
?
-
-
?
azocasein + H2O
?
-
-
-
?
basic membrane protein D + H2O
?
beta-casein + H2O
beta-casein peptide fragments
beta-tubulin + H2O
?
-
-
-
-
?
BODIPY TR-X casein + H2O
?
-
-
-
-
?
bone morphogenetic protein + H2O
?
-
substrate of isoform HTRA1
-
-
?
Bovine serum albumin + H2O
?
chemotaxis signal transduction phosphatase CheX + H2O
?
chloride intracellular channel protein 1 + H2O
?
-
51% cleavage
-
-
?
citrate synthase + H2O
?
-
acts on the thermally unfolded synthase but not on the native form
-
?
clusterin + H2O
?
-
50% cleavage
-
-
?
colicin A lysis protein + H2O
?
-
i.e. pCal, hydrolyses the acylated precursor form, cleaves at two sites near the C-terminal end to give two truncated proteins which are matured into two truncated Cals
-
?
colicin A lysis protein precursor + H2O
?
-
-
-
?
competence-stimulating peptide CSP-1 + H2O
?
-
enzyme HtrA constitutes the primary extracytoplasmic competence-stimulating peptide-degrading activity in cultures of Streptococcus pneumoniae. Both substrate isoforms CSP-1 and CSP-2 interact with HtrA with similar efficiencies
cleavage predominantly follows residue Phe8 of the CSP-1 isoform of the peptide within its central hydrophobic patch
-
?
competence-stimulating peptide CSP-2 + H2O
?
-
enzyme HtrA constitutes the primary extracytoplasmic competence-stimulating peptide-degrading activity in cultures of Streptococcus pneumoniae. Both substrate isoforms CSP-1 and CSP-2 interact with HtrA with similar efficiencies
-
-
?
CSP-1 FRET peptide + H2O
?
-
reporter peptide with incorporation of a QSY-7 quencher and a Cys(Alexa488) fluorophore at theN-erminus andC-terminus of CSP-1, respectively
-
-
?
D1 protein + H2O
?
-
degrades photodamaged D1 protein of photosystem II
-
?
decorin + H2O
?
-
substrate of isoforms HTRA1 and HTRA3
-
-
?
decorin + H2O
decorin peptide fragments
DPMFKLV-4-nitroanilide + H2O
DPMFKLV + 4-nitroaniline
-
-
-
-
?
E-cadherin + H2O
85 kDa N-terminal fragment + 40 kDa C-terminal fragment
-
selective substrate
-
-
?
Faa1p + H2O
?
-
direct interaction of Faa1p with the Omi/HtrA protease orthologue Ynm3p alters lipid homeostasis, Ynm3p modulates fatty acid metabolism and gene regulation through negative regulation of ACSL activity, overview
-
-
?
fascin + H2O
?
-
40% cleavage
-
-
?
fibromodulin + H2O
?
-
90% cleavage
-
-
?
fibronectin + H2O
fibronectin peptide fragments
filamentous haemagglutinin precursor + H2O
?
-
DegP contributes to degrading the filamentous haemagglutinin precursor when it is blocked intracellularly
-
-
?
gamma-tubulin + H2O
?
-
-
-
-
?
glypican-4 + H2O
?
-
-
-
?
HCLS1-associated X1 + H2O
?
-
substrate of isoform HTRA2
-
-
?
HYTAVVKKSSAV + H2O
?
-
model substrate
-
?
IciA protein + H2O
?
-
inhibitor of DNA replication initiation
-
?
insulin beta-chain + H2O
?
insulin growth factor-binding protein 5 + H2O
?
-
substrate of isoform HTRA1
-
-
?
LamB + H2O
?
DegP functions as a geniune chaperone
-
-
?
lambda repressor + H2O
?
-
N-terminal domain
-
?
malate dehydrogenase + H2O
?
MalE + H2O
?
-
periplasmic maltose-binding protein
-
-
?
matrix Gla protein + H2O
processed matrix Gla protein + 12 kDa peptide
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
OmpA + H2O
?
-
outer membrane porin protein
-
-
?
OmpC + H2O
?
-
outer membrane porin protein
-
-
?
OmpF + H2O
?
-
outer membrane porin protein
-
-
?
OmpW + H2O
?
-
outer membrane porin protein
-
-
?
OmpX + H2O
?
-
outer membrane porin protein
-
-
?
osteoprotegerin + H2O
?
-
-
-
?
outer membrane protein + H2O
?
-
-
-
?
outer membrane protein A + H2O
?
in contrast to misfolded model substrates, which are degraded within a few min, the co-purified outer-membrane proteins are stable. Even in the presence of externally applied proteases, the bound outer-membrane proteins are almost entirely resistant to proteolytic degradation. DegP functions as a geniune chaperone
-
-
?
outer membrane protein C + H2O
?
in contrast to misfolded model substrates, which are degraded within a few min, the co-purified outer-membrane proteins are stable. Even in the presence of externally applied proteases, the bound outer-membrane proteins are almost entirely resistant to proteolytic degradation. DegP functions as a geniune chaperone
-
-
?
outer membrane protein F + H2O
?
in contrast to misfolded model substrates, which are degraded within a few min, the co-purified outer-membrane proteins are stable. Even in the presence of externally applied proteases, the bound outer-membrane proteins are almost entirely resistant to proteolytic degradation. DegP functions as a geniune chaperone
-
-
?
PapA + H2O
?
-
major pilin subunit of the Pap pilus
-
?
PMMGKASPV-4-nitroanilide + H2O
PMMGKASPV + 4-nitroaniline
-
-
-
-
?
pro-transforming growth factor-beta1 + H2O
mature transforming growth factor-beta1 + latency-associated peptide
-
-
latency-associated peptide is the N-terminal of pro-transforming growth factor-beta1
-
?
protein tau + H2O
?
-
-
-
?
PVFNTLPMMGKASPV-4-nitroanilide + H2O
PVFNTLPMMGKASPV + 4-nitroaniline
-
-
-
-
?
reaction centre protein D1 + H2O
?
reaction centre protein D2 + H2O
?
reduced alkaline phosphatase + H2O
?
-
-
-
-
?
reduced lysozyme + H2O
?
-
-
-
?
RseA + H2O
?
-
physiological substrate of DegP
-
-
?
SPMFKGV-4-nitroanilide + H2O
SPMFKGV + 4-nitroaniline
-
-
-
-
?
Staphylococcus aureus nuclease Nuc precursor + H2O
?
-
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
?
syndecan-4 + H2O
?
-
-
-
?
talin-1 + H2O
?
-
21% cleavage
-
-
?
tau protein + H2O
?
enzyme degrades aggregated and fibrillar tau, a protein critically involved in various neurological disorders
-
-
?
transforming growth factor-beta + H2O
?
-
substrate of isoform HTRA1
-
-
?
tuberous sclerosis complex 2 protein + H2O
?
-
specific substrate for HtrA1 which is cleaved both in vitro and in vivo
-
-
?
VFNTLPMMGKASPV-4-nitroanilide + H2O
VFNTLPMMGKASPV + 4-nitroaniline
-
-
-
-
?
Vitronectin + H2O
?
-
54% cleavage
-
-
?
additional information
?
-
alpha-casein + H2O
?
-
-
-
-
?
alpha-casein + H2O
?
-
weaker substrate than beta-casein
-
?
alpha-lactalbumin + H2O
?
-
only when incubated in the presence of 20 mM dithiothreitol, which reduces the structural disulfide bonds and unfold the protein, and above 34°C, is CtHtrA able to proteolyse alpha-lactalbumin
-
-
?
alpha-lactalbumin + H2O
?
-
acts on the fully unfolded protein but not on the native form
-
?
alpha-lactalbumin + H2O
?
-
-
-
?
basic membrane protein D + H2O
?
-
-
-
?
basic membrane protein D + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
-
?
beta-casein + H2O
?
-
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
-
?
beta-casein + H2O
?
-
better substrate than alpha-casein
-
?
beta-casein + H2O
?
-
cleaves beta-casein yielding several polypeptide fragments
-
?
beta-casein + H2O
?
-
nonphysiological substrate of DegP
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
?
-
-
-
-
?
beta-casein + H2O
?
-
little uncleaved beta-casein remains in assays with recombinant HtrA, and a prominent degradation fragment appears. Recombinant HhoA completely degrades the excess of beta-casein, whereas recombinant HhoB shows only little activity and generates a prominent degradation fragment with a slightly lower molecular mass than intact beta-casein. The C-terminal cleavage sites Val162, Gln141 and Val130 are identified for recombinant HtrA. A common cleavage site at Leu165 is found for HhoA and HhoB, however, HhoA additionally cleaves beta-casein at Ala101 generating two proteolytic fragments, the N-terminal 1-101 as well as the C-terminal 102-199 amino acid fragments
-
-
?
beta-casein + H2O
?
-
-
-
?
beta-casein + H2O
beta-casein peptide fragments
-
the cleavage activity at 37°C is ATP independent, not significantly influenced by buffer composition, active over a broad range of pH, and with a broad optimum at pH 6.5, and 20 mM salts, e.g. magnesium sulfate, magnesium chloride, or sodium chloride, improving activity, determination of cleavage sites
-
-
?
beta-casein + H2O
beta-casein peptide fragments
-
-
-
-
?
biglycan + H2O
?
-
-
-
-
?
biglycan + H2O
?
-
substrate of isoforms HTRA1 and HTRA3
-
-
?
Bovine serum albumin + H2O
?
-
85% of the activity with casein
-
-
?
Bovine serum albumin + H2O
?
-
85% of the activity with casein
-
-
?
Bovine serum albumin + H2O
?
-
denatured
-
?
casein + H2O
?
-
-
-
-
?
casein + H2O
?
-
major pilin subunit of the Pap pilus
-
?
casein + H2O
?
-
partially unfolded casein
-
?
casein-FITC + H2O
?
-
-
-
?
casein-FITC + H2O
?
-
-
-
?
chemotaxis signal transduction phosphatase CheX + H2O
?
-
-
-
?
chemotaxis signal transduction phosphatase CheX + H2O
?
-
-
-
?
decorin + H2O
decorin peptide fragments
-
a small leucine-rich proteoglycan
-
-
?
decorin + H2O
decorin peptide fragments
-
the small leucine-rich proteoglycan is highly expressed in bone and regulates type I collagen fibril assembly
generation of fragments ranging from 150 to 75 kDa
-
?
decorin + H2O
decorin peptide fragments
-
a small leucine-rich proteoglycan, human substrate
generation of fragments ranging from 150 to 75 kDa
-
?
E-cadherin + H2O
?
-
enzyme cleaves E-cadherin on host cells
-
-
?
E-cadherin + H2O
?
enzyme cleaves E-cadherin on host cells
-
-
?
E-cadherin + H2O
?
enzyme cleaves E-cadherin on host cells
-
-
?
E-cadherin + H2O
?
-
-
-
-
?
E-cadherin + H2O
?
-
enzyme cleaves E-cadherin on host cells
-
-
?
Fibronectin + H2O
?
-
selective substrate
-
-
?
Fibronectin + H2O
?
-
-
-
-
?
Fibronectin + H2O
?
-
substrate of isoform HTRA1
-
-
?
fibronectin + H2O
fibronectin peptide fragments
-
-
-
-
?
fibronectin + H2O
fibronectin peptide fragments
-
HtrA is involved in cartilage catabolism
-
-
?
fibronectin + H2O
fibronectin peptide fragments
-
a major noncollagenous component of mineralized bone matrix
generation of fragments ranging from 200 to 150 kDa
-
?
fibronectin + H2O
fibronectin peptide fragments
-
human plasma-derived substrate
generation of fragments ranging from 200 to 150 kDa
-
?
FkpA + H2O
?
-
periplasmic peptidyl-prolyl cistrans isomerase, chaperone
-
-
?
FkpA + H2O
?
-
periplasmic peptidyl-prolyl cis-trans isomerase, chaperone
-
-
?
Gelatin + H2O
?
-
20% of the activity with casein
-
-
?
Gelatin + H2O
?
-
20% of the activity with casein
-
-
?
insulin beta-chain + H2O
?
-
-
-
-
?
insulin beta-chain + H2O
?
-
oxidized beta-chain which is fully unfolded
-
?
Lysozyme + H2O
?
-
-
-
-
?
Lysozyme + H2O
?
-
can only be digested in the presence of reducing agents
-
?
malate dehydrogenase + H2O
?
-
-
-
-
?
malate dehydrogenase + H2O
?
-
acts on the thermally unfolded protein but not on the native form
-
?
matrix Gla protein + H2O
processed matrix Gla protein + 12 kDa peptide
-
the protein substrate is present in cartilage, bone, and arteries
-
-
?
matrix Gla protein + H2O
processed matrix Gla protein + 12 kDa peptide
-
cleavage of MGP at the C terminus
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
51% of the activity with casein
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
51% of the activity with casein
-
-
?
Protein + H2O
?
-
-
-
?
Protein + H2O
?
-
cleaves between paired valine residues
-
?
Protein + H2O
?
-
cleaves model substrates at discrete Val/Xaa or Ile/Xaa sites
-
?
Protein + H2O
?
-
cleaves preferably at hydrophobic side chains at the P1 position
-
?
Protein + H2O
?
-
denatured proteins aggregate to form a distinct S fraction, one third of the isolated S fraction is converted to trichloroacetic acid-soluble products
-
?
Protein + H2O
?
-
denatured proteins aggregate to form a distinct S fraction, one third of the isolated S fraction is converted to trichloroacetic acid-soluble products, enzyme has a preference for valine or isoleucine as the residue preceding the cleavage site
-
?
Protein + H2O
?
-
enzyme recognizes an ssrA-encoded peptide tag which is tagged to misfolded proteins or protein fragments
-
?
reaction centre protein D1 + H2O
?
-
substrate of isoform DEG1
-
-
?
reaction centre protein D1 + H2O
?
-
substrate of isoform DEG1
-
-
?
reaction centre protein D2 + H2O
?
-
substrate of isoforms DEG1, DEG5, DEG7 and DEG8
-
-
?
reaction centre protein D2 + H2O
?
-
substrate of isoforms DEG1, DEG5, DEG7 and DEG8
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
the enzyme plays a role in the Burkholderia cenocepacia stress response, HtrABCAL2829 is required for growth of Burkholderia cenocepacia upon exposure to osmotic stress by NaCl or KCl, and thermal stress at 44°C
-
-
?
additional information
?
-
-
HtrA sequence specificity, overview, no activity with albumin and myoglobin at 37°C, HtrA acts as both a chaperone and protease, HtrA mutant S247A is able to chaperone insulin B-chain, irrespective of temperature, but at 30°C only HtrA and not mutant S247A displays significant chaperone activity for alpha-lactalbumin, overview
-
-
?
additional information
?
-
-
acts on substrates that are at least partially unfolded, does not cleave stably folded proteins, acts as a general chaperone forming stable complexes with several misfolded proteins
-
?
additional information
?
-
-
no substrates are: bovine serum albumin, ovalbumin, globin, insulin and other peptides that are routinely used as protease substrates
-
?
additional information
?
-
-
no substrates are: native bovine serum albumin, insulin, growth hormone or a variety of commonly used peptide substrates
-
?
additional information
?
-
-
heat shock serine protease that degrades misfolded proteins at high temperatures
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, enzyme is indispensable for bacterial survival at elevated temperatures
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, enzyme is indispensable for bacterial survival at temperatures above 42°C
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, enzyme is indispensable for bacterial survival at temperatures above 42°C
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, participate in removal of aggregated proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, switches from chaperone to protease function in a temperature-dependent manner
-
?
additional information
?
-
-
involved in the degradation of denatured and unfolded proteins
-
?
additional information
?
-
-
involved in the degradation of misfolded proteins
-
?
additional information
?
-
-
involved in the degradation of unfolded proteins
-
?
additional information
?
-
-
unable to cleave inhibitor of apoptosis protein
-
-
?
additional information
?
-
-
allosteric activation of DegP by stress signals during bacterial protein quality control, regulation mechanism, pathway scheme, overview
-
-
?
additional information
?
-
-
HtrA inhibits the unfolded lysozyme substrate aggregation over the range of temperatures at 30-45°C, HtrA is able to bind to the denatured polypeptides and as a consequence limits their ability to form large aggregates, overview, HtrA may protect the bacterial cells from deleterious effects of heat shock not only by degrading the damaged proteins but by combination of the proteolytic and chaperoning activities
-
-
?
additional information
?
-
-
HtrA shows chaperone-like activity, overview
-
-
?
additional information
?
-
-
when unfolded proteins bind to CpxP, DegP efficiently degrades this protein complex
-
-
?
additional information
?
-
-
almost no activity towards SDAEFRHDSGYEV-4-nitroanilide, SDAEFRHDSGYEV-4-nitroanilide, SGRVVPGYGHA-4-nitroanilide, SPLPEGV-4-nitroanilide , GLATGNVSTAELQDATPA-4-nitroanilide, KGKNSGSGATPV-4-nitroanilide, KGASVPGAGLV-4-nitroanilide, SPAKGGEEPLPEGV-4-nitroanilide, and benzoyl-L-Arg-4-nitroanilide
-
-
?
additional information
?
-
-
identification of beta-barrel outer membrane proteins, OMPs, as major natural substrates by photo-crosslinking using non-natural amino acid DiZPK, 3-(3-methyl-3H-diazirine-3-yl)-propaminocarbonyl-Nepsilon-L-lysine, as the photo-crosslinker. Isoform DegP primarily functions as a protease, at both low and high temperatures, to eliminate unfolded outer membrane proteins, with hardly any appreciable chaperone activity in cells. The toxic and cell membrane-damaging misfolded outer membrane proteins would accumulate in DegP-lacking cells cultured under heat shock conditions
-
-
?
additional information
?
-
the enzyme shows chaperone-like activity with substrate lysozyme, and protease activity. The PDZ domains are needed for DegQ chaperone activity. Up to six lysozyme substrates bind inside the DegQ dodecamer cage, binding of a well-ordered lysozyme to four DegQ protomers, overview
-
-
?
additional information
?
-
-
the enzyme does not cleave recombinant immunoglobulin A, epidermal growth factor receptor or junctional adhesion molecule
-
-
?
additional information
?
-
enzyme cuts preferentially after valine at P1, with serine, leucine and isoleucine in decreasing order of preference. No substrate: native lysozyme
-
-
?
additional information
?
-
-
enzyme cuts preferentially after valine at P1, with serine, leucine and isoleucine in decreasing order of preference. No substrate: native lysozyme
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, involved in arthritis, cell growth, stress response, apoptosis and aging, possible tumor suppressor function
-
?
additional information
?
-
-
HtrA1 expression is regulated by cisplatin and paclitaxel, and upregulation results in catalytic activation of HtrA1, overview, HtrA1 influences tumor response to chemotherapy by modulating hemotherapy-induced cytotoxicity, HtrA1 in ovarian and gastric cancers may contribute to in vivo chemoresistance, overview
-
-
?
additional information
?
-
-
HtrA1 plays a role in arthritic disease, within the context of arthritis pathology HtrA1 contributes to cartilage degradation, overview
-
-
?
additional information
?
-
-
HTRA1 does not cleave CFH protein, complement component C3 and complement component C3b
-
-
?
additional information
?
-
-
HTRA1 interacts with presenilin 1 to cleave one product of gamma-secretase
-
-
?
additional information
?
-
-
HTRA1 fails to cleave mature transforming growth factor-beta1
-
-
?
additional information
?
-
-
HtrA1 shows no activity towards tuberous sclerosis complex 1 protein and bovine serum albumin
-
-
?
additional information
?
-
role in extracellular proteolysis, proteolysis occurs during or after export to the cell surface, involved in the degradation of abnormal exported proteins
-
?
additional information
?
-
-
role in extracellular proteolysis, proteolysis occurs during or after export to the cell surface, involved in the degradation of abnormal exported proteins
-
?
additional information
?
-
-
HtrA is involved in the stress response of several important Gram-negative, as well as gram-positive, pathogens, HtrA is required for efficient Listeria monocytogenes biofilm formation at high temperatures
-
-
?
additional information
?
-
-
HtrA is involved in the stress response of several important Gram-negative, as well as gram-positive, pathogens, HtrA is required for efficient Listeria monocytogenes biofilm formation at high temperatures
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, acts as protease, chaperone and regulator of apoptosis
-
?
additional information
?
-
-
HtrA is specific for mature mucosal mast cells
-
-
?
additional information
?
-
-
HtrA1 degrades specific matrix-associated proteins, HtrA1 inhibits mineral deposition by osteoblasts, the protease domain and the PDZ domain are essential for the inhibition of osteoblast mineralization by HtrA1, overview
-
-
?
additional information
?
-
-
HtrA1 is a secreted multidomain protein with serine protease activity
-
-
?
additional information
?
-
-
HtrA is specific for mature mucosal mast cells
-
-
?
additional information
?
-
-
MucD negatively regulates alginate production on genetic level, MucD-deficient strain show temperature-dependent alginate production, while MucD-containing strains do not, which is independent of MucD proteolytic activity, overview
-
-
?
additional information
?
-
-
DegP degrades unfolded or misfolded, secreted, and accumulated inactive proteins and is important for cell survival, especially in the absence of DsbA
-
-
?
additional information
?
-
-
mutants are deficient in their ability to survive in mice or macrophages
-
?
additional information
?
-
the enzyme is involved in survival of the bacteria under heat shock stress conditions in vitro and in vivo, overview
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
enzyme displays a preference for substrates with non-polar residues at the P1 site
-
-
?
additional information
?
-
-
HtrA is essential for the maturation of cysteine protease streptococcal pyrogenic exotoxin B, SpeB, but is unable to directly process SpeB zymogen, proSpeB to the active form in vitro, thus playing an indirect role in the maturation, overview
-
-
?
additional information
?
-
-
no activity with correctly folded globular bovine serum albumin or lysozyme
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, chaperone and proteolytic activity
-
?
additional information
?
-
HtrA1 promotes posterior development in mRNA-injected Xenopus laevis embryos, e.g. induces secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner, HtrA1 activates FGF/ERK signaling and the transcription of FGF genes by cleaving proteoglycans and releasing cell surface-bound FGF ligands, overview
-
-
?
additional information
?
-
-
deletion mutant is unable to grow at an elevated temperature and to survive within macrophages after phagocytosis
-
?
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ADAM9 + H2O
?
-
54% cleavage
-
-
?
aggrecan + H2O
aggrecan fragments
-
the HtrA1-specific cleavage site is VQTV3562357TWPD within the interglobular domain of aggrecan
-
-
?
alpha-tubulin + H2O
?
-
-
-
-
?
alpha2-macroglobulin + H2O
?
-
55% cleavage
-
-
?
beta-tubulin + H2O
?
-
-
-
-
?
biglycan + H2O
?
-
substrate of isoforms HTRA1 and HTRA3
-
-
?
bone morphogenetic protein + H2O
?
-
substrate of isoform HTRA1
-
-
?
chloride intracellular channel protein 1 + H2O
?
-
51% cleavage
-
-
?
clusterin + H2O
?
-
50% cleavage
-
-
?
decorin + H2O
?
-
substrate of isoforms HTRA1 and HTRA3
-
-
?
decorin + H2O
decorin peptide fragments
-
the small leucine-rich proteoglycan is highly expressed in bone and regulates type I collagen fibril assembly
generation of fragments ranging from 150 to 75 kDa
-
?
E-cadherin + H2O
85 kDa N-terminal fragment + 40 kDa C-terminal fragment
-
selective substrate
-
-
?
E-cadherin + H2O
?
-
-
-
-
?
Faa1p + H2O
?
-
direct interaction of Faa1p with the Omi/HtrA protease orthologue Ynm3p alters lipid homeostasis, Ynm3p modulates fatty acid metabolism and gene regulation through negative regulation of ACSL activity, overview
-
-
?
fascin + H2O
?
-
40% cleavage
-
-
?
fibromodulin + H2O
?
-
90% cleavage
-
-
?
fibronectin + H2O
fibronectin peptide fragments
filamentous haemagglutinin precursor + H2O
?
-
DegP contributes to degrading the filamentous haemagglutinin precursor when it is blocked intracellularly
-
-
?
FkpA + H2O
?
-
periplasmic peptidyl-prolyl cistrans isomerase, chaperone
-
-
?
gamma-tubulin + H2O
?
-
-
-
-
?
HCLS1-associated X1 + H2O
?
-
substrate of isoform HTRA2
-
-
?
insulin beta-chain + H2O
?
-
-
-
-
?
insulin growth factor-binding protein 5 + H2O
?
-
substrate of isoform HTRA1
-
-
?
LamB + H2O
?
DegP functions as a geniune chaperone
-
-
?
MalE + H2O
?
-
periplasmic maltose-binding protein
-
-
?
matrix Gla protein + H2O
processed matrix Gla protein + 12 kDa peptide
-
the protein substrate is present in cartilage, bone, and arteries
-
-
?
OmpA + H2O
?
-
outer membrane porin protein
-
-
?
OmpC + H2O
?
-
outer membrane porin protein
-
-
?
OmpF + H2O
?
-
outer membrane porin protein
-
-
?
OmpW + H2O
?
-
outer membrane porin protein
-
-
?
OmpX + H2O
?
-
outer membrane porin protein
-
-
?
outer membrane protein + H2O
?
-
-
-
?
outer membrane protein A + H2O
?
in contrast to misfolded model substrates, which are degraded within a few min, the co-purified outer-membrane proteins are stable. Even in the presence of externally applied proteases, the bound outer-membrane proteins are almost entirely resistant to proteolytic degradation. DegP functions as a geniune chaperone
-
-
?
outer membrane protein C + H2O
?
in contrast to misfolded model substrates, which are degraded within a few min, the co-purified outer-membrane proteins are stable. Even in the presence of externally applied proteases, the bound outer-membrane proteins are almost entirely resistant to proteolytic degradation. DegP functions as a geniune chaperone
-
-
?
outer membrane protein F + H2O
?
in contrast to misfolded model substrates, which are degraded within a few min, the co-purified outer-membrane proteins are stable. Even in the presence of externally applied proteases, the bound outer-membrane proteins are almost entirely resistant to proteolytic degradation. DegP functions as a geniune chaperone
-
-
?
reaction centre protein D1 + H2O
?
reaction centre protein D2 + H2O
?
talin-1 + H2O
?
-
21% cleavage
-
-
?
transforming growth factor-beta + H2O
?
-
substrate of isoform HTRA1
-
-
?
tuberous sclerosis complex 2 protein + H2O
?
-
specific substrate for HtrA1 which is cleaved both in vitro and in vivo
-
-
?
Vitronectin + H2O
?
-
54% cleavage
-
-
?
additional information
?
-
Fibronectin + H2O
?
-
selective substrate
-
-
?
Fibronectin + H2O
?
-
substrate of isoform HTRA1
-
-
?
fibronectin + H2O
fibronectin peptide fragments
-
HtrA is involved in cartilage catabolism
-
-
?
fibronectin + H2O
fibronectin peptide fragments
-
a major noncollagenous component of mineralized bone matrix
generation of fragments ranging from 200 to 150 kDa
-
?
Protein + H2O
?
-
-
-
?
reaction centre protein D1 + H2O
?
-
substrate of isoform DEG1
-
-
?
reaction centre protein D1 + H2O
?
-
substrate of isoform DEG1
-
-
?
reaction centre protein D2 + H2O
?
-
substrate of isoforms DEG1, DEG5, DEG7 and DEG8
-
-
?
reaction centre protein D2 + H2O
?
-
substrate of isoforms DEG1, DEG5, DEG7 and DEG8
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
the enzyme plays a role in the Burkholderia cenocepacia stress response, HtrABCAL2829 is required for growth of Burkholderia cenocepacia upon exposure to osmotic stress by NaCl or KCl, and thermal stress at 44°C
-
-
?
additional information
?
-
-
heat shock serine protease that degrades misfolded proteins at high temperatures
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, enzyme is indispensable for bacterial survival at elevated temperatures
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, enzyme is indispensable for bacterial survival at temperatures above 42°C
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, enzyme is indispensable for bacterial survival at temperatures above 42°C
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, participate in removal of aggregated proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, switches from chaperone to protease function in a temperature-dependent manner
-
?
additional information
?
-
-
involved in the degradation of denatured and unfolded proteins
-
?
additional information
?
-
-
involved in the degradation of misfolded proteins
-
?
additional information
?
-
-
involved in the degradation of unfolded proteins
-
?
additional information
?
-
-
allosteric activation of DegP by stress signals during bacterial protein quality control, regulation mechanism, pathway scheme, overview
-
-
?
additional information
?
-
-
HtrA inhibits the unfolded lysozyme substrate aggregation over the range of temperatures at 30-45°C, HtrA is able to bind to the denatured polypeptides and as a consequence limits their ability to form large aggregates, overview, HtrA may protect the bacterial cells from deleterious effects of heat shock not only by degrading the damaged proteins but by combination of the proteolytic and chaperoning activities
-
-
?
additional information
?
-
-
when unfolded proteins bind to CpxP, DegP efficiently degrades this protein complex
-
-
?
additional information
?
-
-
identification of beta-barrel outer membrane proteins, OMPs, as major natural substrates by photo-crosslinking using non-natural amino acid DiZPK, 3-(3-methyl-3H-diazirine-3-yl)-propaminocarbonyl-Nepsilon-L-lysine, as the photo-crosslinker. Isoform DegP primarily functions as a protease, at both low and high temperatures, to eliminate unfolded outer membrane proteins, with hardly any appreciable chaperone activity in cells. The toxic and cell membrane-damaging misfolded outer membrane proteins would accumulate in DegP-lacking cells cultured under heat shock conditions
-
-
?
additional information
?
-
the enzyme shows chaperone-like activity with substrate lysozyme, and protease activity. The PDZ domains are needed for DegQ chaperone activity. Up to six lysozyme substrates bind inside the DegQ dodecamer cage, binding of a well-ordered lysozyme to four DegQ protomers, overview
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, involved in arthritis, cell growth, stress response, apoptosis and aging, possible tumor suppressor function
-
?
additional information
?
-
-
HtrA1 expression is regulated by cisplatin and paclitaxel, and upregulation results in catalytic activation of HtrA1, overview, HtrA1 influences tumor response to chemotherapy by modulating hemotherapy-induced cytotoxicity, HtrA1 in ovarian and gastric cancers may contribute to in vivo chemoresistance, overview
-
-
?
additional information
?
-
-
HtrA1 plays a role in arthritic disease, within the context of arthritis pathology HtrA1 contributes to cartilage degradation, overview
-
-
?
additional information
?
-
-
HTRA1 does not cleave CFH protein, complement component C3 and complement component C3b
-
-
?
additional information
?
-
-
HTRA1 interacts with presenilin 1 to cleave one product of gamma-secretase
-
-
?
additional information
?
-
role in extracellular proteolysis, proteolysis occurs during or after export to the cell surface, involved in the degradation of abnormal exported proteins
-
?
additional information
?
-
-
role in extracellular proteolysis, proteolysis occurs during or after export to the cell surface, involved in the degradation of abnormal exported proteins
-
?
additional information
?
-
-
HtrA is involved in the stress response of several important Gram-negative, as well as gram-positive, pathogens, HtrA is required for efficient Listeria monocytogenes biofilm formation at high temperatures
-
-
?
additional information
?
-
-
HtrA is involved in the stress response of several important Gram-negative, as well as gram-positive, pathogens, HtrA is required for efficient Listeria monocytogenes biofilm formation at high temperatures
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, acts as protease, chaperone and regulator of apoptosis
-
?
additional information
?
-
-
HtrA is specific for mature mucosal mast cells
-
-
?
additional information
?
-
-
HtrA1 degrades specific matrix-associated proteins, HtrA1 inhibits mineral deposition by osteoblasts, the protease domain and the PDZ domain are essential for the inhibition of osteoblast mineralization by HtrA1, overview
-
-
?
additional information
?
-
-
HtrA is specific for mature mucosal mast cells
-
-
?
additional information
?
-
-
MucD negatively regulates alginate production on genetic level, MucD-deficient strain show temperature-dependent alginate production, while MucD-containing strains do not, which is independent of MucD proteolytic activity, overview
-
-
?
additional information
?
-
-
DegP degrades unfolded or misfolded, secreted, and accumulated inactive proteins and is important for cell survival, especially in the absence of DsbA
-
-
?
additional information
?
-
-
mutants are deficient in their ability to survive in mice or macrophages
-
?
additional information
?
-
the enzyme is involved in survival of the bacteria under heat shock stress conditions in vitro and in vivo, overview
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
HtrA is essential for the maturation of cysteine protease streptococcal pyrogenic exotoxin B, SpeB, but is unable to directly process SpeB zymogen, proSpeB to the active form in vitro, thus playing an indirect role in the maturation, overview
-
-
?
additional information
?
-
-
involved in the degradation of damaged proteins
-
?
additional information
?
-
-
involved in the degradation of damaged proteins, chaperone and proteolytic activity
-
?
additional information
?
-
HtrA1 promotes posterior development in mRNA-injected Xenopus laevis embryos, e.g. induces secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner, HtrA1 activates FGF/ERK signaling and the transcription of FGF genes by cleaving proteoglycans and releasing cell surface-bound FGF ligands, overview
-
-
?
additional information
?
-
-
deletion mutant is unable to grow at an elevated temperature and to survive within macrophages after phagocytosis
-
?
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evolution
the enzyme belongs to the HtrA protein family combines chaperone and protease activities and is essential for protein quality control in many organisms. HtrA proteins are composed of a chymotrypsin-like protease domain and one (DegS, HTRA1, HTRA2) or two PDZ domains (DegP, DegQ)
metabolism
-
one of the targets of HtrA1 activity during fetal development is the tuberous sclerosis complex 2-tuberous sclerosis complex 1 pathway
additional information
enzyme structure analysis of the 12 and 24mer states in complex with model substrates, overview. DegQ PDZ domains are located adjacent to substrate density and their presence is required for chaperone activity
malfunction
-
Bordetella pertussis lacking the periplasmic chaperone/protease DegP has a strong growth defect at 37°C, and the integrity of its outer membrane is compromised
malfunction
-
HTRA1 is involved in complement regulation and amyloid deposition in age-related macular degeneration pathogenesis
malfunction
-
lack of nuclear localisation of Nma111p causes late onset of cell death during chronological ageing
malfunction
-
stable knockdown of HtrA1 in SKOV-3 and TOV-21G cells results in resistance to anoikis due to enhanced activation of epidermal growth factor receptor/AKT pathway. Downregulation of HtrA1 significantly enhances the peritoneal dissemination of SKOV-3ip1 cells in nonobese diabetic/severe combined immunodeficient mice
malfunction
-
cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy-associated mutant HTRA1 decreases protease activity and fails to decrease transforming growth factor-beta family signaling
malfunction
-
disruption of the high temperature requirement A gene affects significantly the ability of the resulting mutants to withstand heat, oxidative, ethanol and osmotic stress, exhibit delayed proliferation, and show a decrease of over six orders of magnitude in virulence as compared with the parental wild type strain
malfunction
-
HTRA1 knockout mice display reduced blood vessel in retina and upregulation of growth differentiation factor 6
malfunction
-
the loss of HTRA activity is correlated with severe diseases, including arthritis, cancer, familial ischemic cerebral smallvessel disease and age-related macular degeneration, as well as Parkinsons disease and Alzheimers disease
malfunction
-
disruption of the high temperature requirement A gene affects significantly the ability of the resulting mutants to withstand heat, oxidative, ethanol and osmotic stress, exhibit delayed proliferation, and show a decrease of over six orders of magnitude in virulence as compared with the parental wild type strain
-
physiological function
-
DegP can act as a chaperone and a protease at the same time. Deg P is a key player in extracytoplasmic protein quality control and exhibits features of a protective factor during protein folding stress. As a chaperone, DegP refolds periplasmic amylase MalS and the artificial substrate citrate synthase
physiological function
DegP enhances the ability of TX01 to disseminate in fish blood at the advanced stage of infection, heightenes the activity of type 2 autoinducer, and increases the expression of luxS and the genes encoding components of the virulence-associated type III secretion system
physiological function
DegP is a periplasmic heat-shock protein and a key component of protein quality control in the bacterial envelope. DegP along with the Skp chaperone functions to rescue outer membrane proteins that escape recognition by SurA. At temperatures below 28°C, DegP is able to protect misfolded proteins from forming aggregates, whereas at temperatures above 30°C, misfolded proteins are efficiently degraded by DegP
physiological function
-
DegP is critical for growth and for membrane integrity of Bordetella pertussis at 37°C. The chaperone activity of DegP markedly alleviates the periplasmic stress, DegP chaperones the extended filamentous haemagglutinin polypeptide in the periplasm and is thus involved in the two-partner secretion pathway
physiological function
-
HtrA is involved in the ability of the pneumococcus to grow at high temperature, to resist oxidative stress and control the bacteriocin activity
physiological function
-
HtrA plays an important role during acid stress in Streptococcus mutans
physiological function
-
HtrA1 is a critical protease involved in proteoglycan turnover and cartilage degradation during degenerative joint disease
physiological function
-
HtrA1 is implicated in trophoblast cell migration and invasion, tumor progression, chemotherapy-induced cytotoxicity, osteoarthritis, age-related macular degeneration, and pathogenesis of Alzheimer's disease
physiological function
-
HtrA1 is involved in the inhibition of tumor growth factor-beta signaling in endometrial tissues
physiological function
-
HtrA1 modulates microtubule stability and cell motility. In vitro, purified HtrA1 promotes microtubule assembly
physiological function
-
HtrA1 protease activity is required for inhibition of epidermal growth factor receptor signaling
physiological function
-
HtrA3 plays an important role in ovarian development, granulosa cell differentiation and luteinization
physiological function
-
Nma111p functions as a nuclear serine protease that is necessary for apoptosis under cellular stress conditions, such as elevated temperature or treatment of cells with hydrogen peroxide to induce cell death. The nuclear localisation of Nma111p is required for its function in response to oxidative stress
physiological function
-
the chaperone activity but not the protease activity of DegP is required for growth of ssrA-deficient cells at high themperature
physiological function
-
HtrA acts as chaperone and protease
physiological function
-
HtrA acts as chaperone and protease. DEG1 interacts with the reaction centre protein D2 and assists in the assembly of photosystem II
physiological function
-
HtrA acts as chaperone and protease. DEG1 interacts with the reaction centre protein D2 and assists in the assembly of photosystem II
physiological function
-
HtrA displays both temperature-dependent chaperone and protease activities. The HtrA chaperone activity is sufficient for growth at high temperature or under oxidative stress (in the presence of H2O2, cumene hydroperoxide or paraquat) whereas the HtrA protease activity is essential only under conditions close to the growth limit for Campylobacter jejuni. However, the protease activity is required to prevent induction of the cytoplasmic heat shock response even under optimal growth conditions. HtrA may protect oxidatively damaged proteins
physiological function
-
HtrA is a major virulence determinant of Bacillus anthracis
physiological function
-
HtrA is a secreted virulence factor from Helicobacter pylori, which cleaves the ectodomain of E-cadherin. the E-cadherin shedding disrupts epithelial barrier functions allowing Helicobacter pylori to access the intercellular space
physiological function
-
HtrA is important for the biogenesis of extracellular proteins and thus for biofilm formation
physiological function
-
HtrA, HhoA and HhoB are important for survival of Synechocystis sp. strain PCC 6803 under high light and temperature stresses. The three proteases may act as protein-quality-control factors degrading denatured and damaged proteins
physiological function
-
HTRA1 decreases transforming growth factor-beta1 signaling triggered by pro-transforming growth factor-beta1 in the intracellular space
physiological function
-
HTRA1 plays a critical role in the regulation of angiogenesis via transforming growth factor-beta signaling
physiological function
-
isoform HTRA1 regulates cell proliferation by sequestering or proteolysing transforming growth factor-beta and bone morphogenetic protein, proteins of the transforming growth factor family, and IGF-binding protein 5 in the extracellular matrix. Isoform HTRA2 affects quality control in the intermembrane space of mitochondria. HTRA1 and HTRA2 are implicated in tumor suppression and in the control of proliferation, migration and neurodegeneration. Isoform HTRA3 is implicated in pregnancy, and endometrial and ovarian cancers55
physiological function
-
Chlamydia trachomatis isolates with distinct replicative phase growth kinetics show significant loss of viable infectious progeny after HtrA is inhibited during the replicative phase
physiological function
enzyme is not able to complement an Escherichia coli DegP deletion mutant
physiological function
-
HtrA deletion leads to severe defects in E-cadherin cleavage, loss of cell adherence, paracellular transmigration, and basolateral invasion. A conserved pocket in the active center exhibits pronounced proteolytic activity
physiological function
HtrA deletion mutant shows enhanced virulence in the Golden Syrian hamster model of acute Clostridium difficile infection. Deletion of HtrA shows a pleiotropic effect on the transcriptome of Clostridium difficile, including upregulation of the toxin A gene. The mutant shows reduced spore formation and adherence to colonic cells
physiological function
-
HtrA mutants stimulate the blp locus which is responsible for the regulation and secretion of pneumococcal bacteriocins at lower cell density and to a greater extent than strains expressing wild-type HtrA. This effect is not due to direct proteolytic degradation of secreted pheromone by the protease, but instead is a result of HtrA-mediated disruption of peptide processing and secretion. HtrA restricts pneumocin production to high cell density by limiting the rate of accumulation of BlpC, a p eptide pheromone involved in quorum sensing, in the environment. HtrA does not interfere with the ability of a strain to sense environmental pheromones
physiological function
Plasmodium falciparum PfDegP complements the growth defect of the temperature sensitive DegP-deficient mutant in Escherichia coli and imparts resistance to non-permissive temperatures and oxidative stress. DegP exists as a complex with parasite-encoded heat shock protein 70, iron superoxide dismutase and enolase
physiological function
a HtrA knockout strain shows increased sensitivity to stress such as elevated temperature, pH and osmotic shock, as well as treatment with puromycin
physiological function
antibody 94,obtained from a human antibody phage display library, forms a distinct macromolecular complex with HtrA1 and inhibits the enzymatic activity of recombinant and native HtrA1 forms. The 246-kDa complex between the HtrA1 catalytic domain trimer and Fab94 has a propeller-like organization with one Fab bound peripherally to each protomer. The antibody binds to the surface-exposed loops B and C of the catalytic domain. The the HtrA1 catalytic domain-IgG94 complex (636 kDa) is a cage-like structure with three centrally located IgG94 molecules coordinating two the HtrA1 catalytic domain trimers and the six active sites pointing into the cavity of the cage. In both complexes, all antigen-recognition regions bind one HtrA1 protomer and all protomers are bound by a antigen-recognition region
physiological function
combined deletion of the Deg proteases Htra, HhoA and HhoB leads to the down-regulation of proteins related to the biosynthesis of outer cell layers and affects protein secretion. During the late growth phase of the culture, Deg proteases are secreted to the extracellular medium of the wild-type strain. Deletion of the three proteases influences the proteome and metabolome of the whole cell
physiological function
-
disruption of the DegP gene does not impact the parasite lytic cycle in vitro but affects virulence in mice. In a type I strain, DegP appears dispensable for the establishment of an infection, but removal of DegP in a type II strain dramatically impairs the virulence. Type II parasites lacking DegP activity kill immunodeficient mice as efficiently as the wild-type strain
physiological function
HtrA may be important for stress tolerance and physiology of Campylobacter jejuni. Reexpression of the HtrA wild-type gene in HtrA knockout mutants restores Campylobacter jejuni growth at high temperature, growth under high oxygen stress conditions, expression of proteolytically active HtrA oligomers, secretion of HtrA into the supernatant, cell attachment and invasion, and transmigration across polarized epithelial cells
physiological function
HTRA1 degrades amyloid fibrils in an ATP-independent manner. HTRA1 solubilizes protein fibrils and disintegrates the fibrillar core structure. The proteolytic mechanism of HTRA1 toward fibrils is initiated along the entire length of amyloid fibrils, comprises disintegration of tightly packed regions, which is enhanced by the binding of specific sequences by the C-terminal PDZ domain, and ultimately results in the efficient proteolytic removal of amyloid fibrils. Deletion of the PDZ domain severely impairs the destabilization of tau fibrils in vitro and in cultured cells
physiological function
HtrA1 is an osteoprotegerin-degrading enzyme. Inhibitory activity of osteoprotegerin on receptor activator of NF-kappaB ligand RANKL-induced osteoclastogenesis is suppressed by adding HtrA1 in RAW 264.7 cell cultures
physiological function
over a period of 8 h, actively dividing Helicobacter pylori cells secrete HtrA at a similar rate, on average about 9600 HtrA molecules per cell. The amount of secreted HtrA is relatively constant during logarithmic growth in liquid culture, with only a slight increase by a factor of 1.5 over time. In the presence of eukaryotic host cells, the amount of HtrA secreted per bacterium remaines also constant, but the overall secretion level is reduced
physiological function
siRNA-mediated knockdown of HtrA1 and a specific HtrA1 inhibitor reduce apolipoprotein-E 25-kDa fragment formation by 41 and 86%, respectively. HtrA1 regulates reduce apolipoprotein-E 25-kDa fragment formation under physiological conditions
physiological function
A0A2B6C4K2
the proteolytic activity of HtrA is essential for its N-terminal autolysis and subsequent release into the extracellular milieu, while the PDZ domain is dispensable for this process. The PDZ domain appears to be dispensable for most of the functions related to stress resilience as well as involvement of HtrA in assembly of the bacterial S-layer. The proteolytic activity, but not the PDZ domain, appears to be dispensable for the role of HtrA in mediating upregulation of the extracellular protease NprA under starvation stress. In a murine model of anthrax, the HtrA PDZ domain is dispensable for manifestation of Bacillus anthracis virulence
physiological function
-
HtrA is a major virulence determinant of Bacillus anthracis
-
physiological function
-
HtrA displays both temperature-dependent chaperone and protease activities. The HtrA chaperone activity is sufficient for growth at high temperature or under oxidative stress (in the presence of H2O2, cumene hydroperoxide or paraquat) whereas the HtrA protease activity is essential only under conditions close to the growth limit for Campylobacter jejuni. However, the protease activity is required to prevent induction of the cytoplasmic heat shock response even under optimal growth conditions. HtrA may protect oxidatively damaged proteins
-
physiological function
-
HtrA deletion mutant shows enhanced virulence in the Golden Syrian hamster model of acute Clostridium difficile infection. Deletion of HtrA shows a pleiotropic effect on the transcriptome of Clostridium difficile, including upregulation of the toxin A gene. The mutant shows reduced spore formation and adherence to colonic cells
-
physiological function
-
over a period of 8 h, actively dividing Helicobacter pylori cells secrete HtrA at a similar rate, on average about 9600 HtrA molecules per cell. The amount of secreted HtrA is relatively constant during logarithmic growth in liquid culture, with only a slight increase by a factor of 1.5 over time. In the presence of eukaryotic host cells, the amount of HtrA secreted per bacterium remaines also constant, but the overall secretion level is reduced
-
physiological function
-
enzyme is not able to complement an Escherichia coli DegP deletion mutant
-
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decamer
-
10 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer
heptamer
-
7 * 46000, mass spectroscopy
homooligomer
DegP of Escherichia coli assembles into large homooligomers with an internal cavity combining both chaperone and protease activity
pentamer
-
5 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer
?
-
x * 37000, SDS-PAGE
?
-
x * 37000, SDS-PAGE
-
?
-
x * 35000, recombinant wild-type and mutant enzyme, SDS-PAGE
?
-
x * 70000, about 70000 kDa, GST-tagged enzyme, SDS-PAGE
?
-
x * 104000, calculated from sequence for full-length protein, x * 60000, mature C-terminal fragment, * 40000, mature N-terminal fragment, SDS-PAGE
dodecamer
-
12 * 50000, SDS-PAGE, enzyme can exist in two oligomeric forms which are interconvertible
dodecamer
-
consists of two stacks of hexameric rings, SDS-PAGE, cross-linking experiments
dodecamer
12 * 44835, mass spectrometry
hexamer
-
6 * 50000, SDS-PAGE
hexamer
-
6 * 44000, gel filtration, SDS-PAGE
hexamer
-
6 * 46000, mass spectroscopy
hexamer
-
6 * 50000, SDS-PAGE, enzyme can exist in two oligomeric forms which are interconvertible
hexamer
-
6 * 54000, SDS-PAGE, enzyme exists in 3 different forms: pentamer, hexamer and decamer
hexamer
formed by staggered association of trimeric rings
hexamer
-
two trimeric rings for a functional DegP hexamer
hexamer
two trimers for a hexameric structure by staggered association
hexamer
crystal structures suggest that HtrA proteins differ in their molecular architecture, ranging from trimers with surface-accessible active sites to hexamers
hexamer
purified wild type DegP exists mainly as hexamers (dimers of trimers) in solution
hexamer
6 * 44835, mass spectrometry
hexamer
DegP, dimer of two trimers
hexamer
-
two trimers form a hexamer, crystallization experiments
multimer
-
DegP activates its chaperone and protease functions via formation of large cage-like 12- and 24-mers after binding to substrate proteins. Cryo-electron microscopic and biochemical studies reveal that both oligomers are consistently assembled by blocks of DegP trimers, via pairwise PDZ1-PDZ2 interactions between neighboring trimers. Such interactions simultaneously eliminate the inhibitory effects of the PDZ2 domain. Additionally, both DegP oligomers are also observed in extracts of Escherichia. coli cells, strongly implicating their physiological importance
multimer
gel filtration shows three DegP oligomers, namely the 6-mer (DegP6), the 12-mer (DegP12) and the 24-mer (DegP24), of which the two larger particles had additional proteins bound. Binding of misfolded proteins transforms hexameric DegP into large, catalytically active 12-meric and 24-meric multimers. A structural analysis of these particles reveal that DegP represents a protein packaging device whose central compartment is adaptable to the size and concentration of substrate. Moreover, the inner cavity serves antagonistic functions
oligomer
-
largest complexes are dodecamers, probably formed by dimerization of trimers, gel filtration experiments
oligomer
-
DegP protease chaperone system is regulated by oligomer conversion from the resting hexamer into the catalytically active 12mer and 24mer that capture and digest misfolded proteins
oligomer
-
x * about 50000, SDS-PAGE. In the absence of substrate, DegP oligomerizes as a hexameric cage but in its presence DegP reorganizes into active 12- and 24-mer cages. The size of the substrate molecule is the main factor conditioning the oligomeric state adopted by the enzyme, while ther factors such as temperature, do not influence the oligomeric state
tetracosamer
-
-
tetracosamer
24 * 44835, mass spectrometry
trimer
-
-
trimer
crystal structures suggest that HtrA proteins differ in their molecular architecture, ranging from trimers with surface-accessible active sites to hexamers
trimer
3 * 36000, calculated from sequence
trimer
-
does not form hexamers like the Escherichia coli protein
additional information
-
secondary structures for activated protease at different temperatures
additional information
DegQ changes its oligomeric state from hexamers to either 12 or 24mers depending on the concentration of unfolded substrate, DegQ forms 12mers in the absence of substrate at acidic pH, enzyme structure analysis of the 12 and 24mer states in complex with model substrates, overview. The polypeptides are probably cooperatively bound by PDZ1 and protease domains
additional information
the Deg protein contain a catalytic domain with the serine protease catalytic triad, and C-terminal PDZ domains, two in DegP and DegQ, one in DegS
additional information
-
HtrA contains a putative N-terminal membrane anchor domain, Ile13 to Ile26, a trypsin-like serine protease catalytic triad formed by His129, Asp158, and Ser240, and a single C-terminal PDZ domain, Gly296 to Arg385
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E476A
mutation in loop HI residue, completely abolishes proteolytic activity
E485A
mutation of residue located at shared interfaces between protein tetramers, disrupts the octamer in tetramers
F477del/D478del/A479del
deletion of residues of loop HI, mutant protein still forms an octamer structure
K482A
mutation of residue located at shared interfaces between protein tetramers, disrupts the octamer in tetramers
P579A
mutation of residue located at shared interfaces between protein tetramers, mutant still forms an octamer but shows reduced proteolytic activity
Q145A
mutation of residue located at shared interfaces between protein tetramers, mutant still forms an octamer but shows reduced proteolytic activity
Q149A
mutation of residue located at shared interfaces between protein tetramers, mutant still forms an octamer but shows reduced proteolytic activity
Q334A
mutation of residue located at shared interfaces between protein tetramers, disrupts the octamer in tetramers
Q338A
mutation of residue located at shared interfaces between protein tetramers, disrupts the octamer in tetramers
R146A
mutation of residue located at shared interfaces between protein tetramers, mutant still forms an octamer but shows reduced proteolytic activity
R359A
mutation of residue located at shared interfaces between protein tetramers, disrupts the octamer in tetramers
R361A
mutation of residue located at shared interfaces between protein tetramers, disrupts the octamer in tetramers
S266A
mutation introduced to avoid self-degradation during crystallization
S292A
mutation introduced to avoid self-degradation during crystallization
S255A
A0A2B6C4K2
mutation of the catalytic serine residue. Mutant protein does not undergo autolysis and much more abundant in the cellular fraction and almost undetected in its secreted form
S237A
-
protease-inactive mutant
S245A
-
site-directed mutagenesis, and deletion of the HtrABCAL2829 PDZ domains result in an inactive mutant
S197A
-
protease-negative mutant protein with decreased chaperone activity compared to the wild type
S197A
-
protease-negative mutant protein with decreased chaperone activity compared to the wild type
-
S247A
-
proteolytically inactive mutant
C57A/C69A
-
mutant represents a completely reduced HtrA being unable to form the intramolecular S-S bond. Mutant very efficiently degrades alkaline phosphatase at 20°C which is very pronounced compared to wild-type. Thus, the reduction of HtrAs disulfide bridge may facilitate the activation of the protease
C57S/C69S
-
mutant enzyme is less stable, in contrast to wild-type enzyme the mutant protein is autocleaved even without reducing agents
D221A
mutant displays reduced peptide Tyr-Tyr-Phe-stimulated cleavage activity
D221A/H198P
mutant displays more than 65% of peptide Tyr-Tyr-Phe-stimulated cleavage activity of mutant H198P
D232V
-
the mutation causes a stimulation of proteolytic activity
D52A
mutation within LA loop. Mutation has no impact on the proteolytic activity of HtrA
D53A
mutation within LA loop. Mutation has no impact on the proteolytic activity of HtrA
DELTA360-448
-
mutant lacking the PDZ2 domain. Results of gel filtration reveal that the removal of the whole PDZ2 domain, results in the formation of only trimers that form neither the hexamers nor the 12- or 24-mers. Such a mutant trimeric form of DegP exhibits both chaperone-like and protease activities at a level comparable to that of the wild-type protein. Mutant shows no concentration effect compared to wild-type
DELTA440-448
-
the removal of the beta26 strand on the C terminus of the PDZ2 domain (residues 440-448), which is shown to directly interact with the neighboring PDZ1 domain, does not disrupt the formation of DegP hexamers but prevents their conversion to the 12- or 24-mers. Mutant protein exhibits significantly lower chaperone-like and protease activity, suggesting an inhibitory role of the PDZ2 domain for DegP to exhibit chaperone and protease activities. Mutant shows no concentration effect compared to wild-type
F220A
mutant has almost no cleavage activity
F234A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
F46Y
mutation within LA loop. Mutant displays increased activity with substrate beta-casein
F49Y/F50Y
mutation within LA loop. Mutant displays increased activity with substrate beta-casein
F50W/S210A
catalytically inactive mutant
F56S
mutation within LA loop. Mutation has no impact on the proteolytic activity of HtrA
F63Y
mutation within LA loop. Mutant displays increased activity with substrate beta-casein
F68Y
mutation within LA loop. Mutant displays increased activity with substrate beta-casein
H105R
-
loss of protease activity, no change in secondary structure
H198P
mutation stabilizes active DegS and increases the proteolytic activity of otherwise wild-type DegSabout 6-fold under assay conditions
I179A
mutant displays reduced peptide Tyr-Tyr-Phe-stimulated cleavage activity
I179A/H198P D221A
mutant displays more than 65% of peptide Tyr-Tyr-Phe-stimulated cleavage activity of mutant H198P
I228D
-
the mutation does not markedly affect the proteolytic activity of HtrA
I232A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
I238N
-
the proteolytic activity of the mutant enzyme is undetectable
K305A/K379A/K381A/K416A
to monitor directly the influence of the PDZ domains on lipid binding, mutants in which the surface-exposed lysine residues are replaced by alanine: Dose-response experiments reveal that the lipid affinity of the DegP 24-mer mutant is significantly decreased. Thus DegP could function as a periplasmic macropore, allowing the protected diffusion of outer-membrane protein precursors from the inner membrane to the outer membrane
K379E/K381E/K416E
to monitor directly the influence of the PDZ domains on lipid binding, mutants in which the surface-exposed lysine residues are replaced by glutamate alanine: Dose-response experiments reveal that the lipid affinity of the DegP 24-mer mutant is almost entirely impaired. Thus DegP could function as a periplasmic macropore, allowing the protected diffusion of outer-membrane protein precursors from the inner membrane to the outer membrane
K455A
-
proteolytically inactive
L164A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
L229N
-
the proteolytic activity of the mutant enzyme is undetectable
N197A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
N235I
-
the proteolytic activity of the mutant enzyme is undetectable
P161A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
P43G
mutation within LA loop. At 20 °C the activities of are similar to wild-type, whereas at higher temperatures of 35 or 45 °C the mutant shows a higher activity
Q187A
mutant displays reduced peptide Tyr-Tyr-Phe-stimulated cleavage activity
Q187A/H198P
mutant displays more than 65% of peptide Tyr-Tyr-Phe-stimulated cleavage activity of mutant H198P
Q191A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
Q47L
mutation within LA loop. Mutant displays increased activity with substrate beta-casein
Q64A
mutation within LA loop. Mutation has no impact on the proteolytic activity of HtrA
Q64I
mutation within LA loop. Mutation has no impact on the proteolytic activity of HtrA
Q70A
mutation within LA loop. Mutant displays increased activity with substrate beta-casein
R178A
mutant has almost no cleavage activity
R178A/H198P
9-24% of wild-type activity
R44A
mutation within LA loop. Mutation leads to dramatic autocleavage of the protein, occurring both within cells and during their preparation
R44A/F50W/S210A
catalytically inactive mutant
S54A
mutation within LA loop. Mutation has no impact on the proteolytic activity of HtrA
T167V
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
T169A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
Y162A
mutagenic analysis of allosteric activation, less than 2% of peptide Tyr-Tyr-Phe-stimulated wild-type activity
S236A
-
inactive mutant
-
S187A
a proteolytically inactive hexameric enzyme mutant
S210A
proteolytically inactive
S221A
loss of catalytic activity
S326A
mutation in active site, inactive
S217A
-
MucD altered in the protease motif is defective in temperature resistance and alginate gene regulation
E384K
-
naturally occuring single missense mutation in Rhodobacter capsulatus DsbA-null mutant revertant, the mutation leads to decreased protease activity, structural model
G217S
-
naturally occuring single missense mutation in Rhodobacter capsulatus DsbA-null mutant revertant, the mutation leads to decreased protease activity, structural model
G392C
-
naturally occuring single missense mutation in Rhodobacter capsulatus DsbA-null mutant revertant, the mutation leads to decreased protease activity, structural model
I120F
-
naturally occuring single missense mutation in Rhodobacter capsulatus DsbA-null mutant revertant, the mutation leads to decreased protease activity, structural model
L373S
-
naturally occuring single missense mutation in Rhodobacter capsulatus DsbA-null mutant revertant, the mutation leads to decreased protease activity, structural model
L382P
-
naturally occuring single missense mutation in Rhodobacter capsulatus DsbA-null mutant revertant, the mutation leads to decreased protease activity, structural model
V356M
-
naturally occuring single missense mutation in Rhodobacter capsulatus DsbA-null mutant revertant, the mutation leads to decreased protease activity, structural model
T230P
-
mutant P1388 with ciaH mutation
S237A
-
inactive mutant of HhoA
S258A
-
inactive mutant of HhoB
S296A
-
inactive mutant of HtrA
S198A
loss of proteolytic activity
S198A
-
loss of proteolytic activity
-
S210A
-
no proteolytic activity
S210A
-
loss of protease activity, no change in secondary structure
S210A
-
proteolytically inactive
S210A
proteolytically inactive
S210A
-
active site residue mutant, proteolytically inactive HtrA
S210A
-
proteolytically inactive mutant
S210A
-
experimental studies are carried out using protease deficient mutant
S210A
-
mutant S210A, lacking proteolytic activity but retaining chaperone activity is overexpressed in mutant strain htrAdsbA, lacking the functional htrA gene and the functional DsbA/DsbB oxidoreductase system, which: the presence of mutant S210A increases the survival rate of the htrAdsbA double-mutant bacteria. Thus, the proteolytic activity of HtrA seems to play an important role in bacterial cells even at low temperatures (30°C), at which the chaperone function is proposed as being dominant
S210A
proteolytic activity-null mutant
S210A
proteolytically inactive, the mutant does not show significant changes of the secondary structure at the temperature range 20-45°C
S210A
-
the mutant lacks protease activity but retains chaperone activity
S328A
-
enzymatically inactive
S328A
-
active site mutant
S328A
-
site-directed mutagenesis, catalytically inactive mutant HtrA1
S328A
-
the mutation abolishes protease activity in HTRA1
S234A
-
mutant P1386, release of competence inhibition when mutant P1388 is present alone
S234A
-
catalytic site variant
additional information
A0A2B6C4K2
generation of a truncated variant lacking the PDZ domain. Variant is abundantly secreted and processed
additional information
-
generation of a truncated variant lacking the PDZ domain. Variant is abundantly secreted and processed
additional information
-
insertional inactivation of the six-gene cluster in chromosome 1 including gene htrA by recombination with a suicide plasmid, pGPOMEGATp, genetic analyses and complementation studies, overview, inactivation of the htrA gene is associated with a bacterial survival defect in vivo in a rat agar bead model of chronic lung infection
additional information
-
mutant strain NCTC11168deltahtrA cat, main part of the HtrA gene replaced
additional information
-
mutant strain NCTC11168deltahtrA cat, main part of the HtrA gene replaced
-
additional information
-
degP null mutant strain CLC198
additional information
-
disruption of the ptd gende to obtain lack of activity of protease Do, mutant with prolonged lag period, reduced ability to degrade cell proteins and unable to survive at high temperatures
additional information
-
Escherichia coli HtrA mutant
additional information
-
HtrA mutant S210A suppresses the temperature-sensitive phenotype of the htrA mutant and alleviates the lethality of htrA bacteria at high temperatures
additional information
-
a htrAdsbA double-mutant, lacking the functional htrA gene and the functional DsbA/DsbB oxidoreductase system shows a dramatically inhibited growth rate in the presence of 7 mM DTT when compared to mutant strain dsbA containing a single mutation in the DsbA/DsbB oxidoreductase system, indicating that htrA gene is important for survival of the dsbA mutant in the reducing environment
additional information
in degP-null mutant strains the levels of outer-membrane protein A, outer-membrane protein F and to smaller extent also outer-membrane protein C are decreased, indicating an active role of DegP in the outer-membrane protein biogenesis
additional information
-
deletion of region D232 abolishes the activity
additional information
-
downregulation of HtrA1 inovarian cancer attenuates cisplatin- and paclitaxel-induced cytotoxicity, while overexpression of HtrA1 enhances cisplatin- and paclitaxel-induced cytotoxicity
additional information
-
a genetic variation (-625G>A, rs11200638) at the HTRA1 gene promoter locus is associated with spinal disc degeneration
additional information
-
high-temperature requirement A-1 gene promoter polymorphism (G512A) is associated with age-related macular degeneration
additional information
-
HTRA1 rs11200638 gene polymorphisms confer a 10.1fold risk of age-related macular degeneration disease in the Hungarian population
additional information
-
the single nucleotide polymorphisms rs2672598, rs1049331, and rs2293870 in the HRTA1 gene are associated with age-related macular degeneration
additional information
-
mutations in the HTRA1 gene cause a hereditary cerebral small-vessel disease, cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy
additional information
-
htrA mutation in Lactococcus lactis (strain NZ9000htrA) leads to significant reduction of the efficiency of recombinant-protein secretion, as shown by recombinantly expressed streptokinase and nuclease in Lactococcus lactis
additional information
-
a htrA-deletion mutant strain shows reduced biofilm formation at high temperatures and gain sensitivity to puromycin, overview
additional information
-
a htrA-deletion mutant strain shows reduced biofilm formation at high temperatures and gain sensitivity to puromycin, overview
-
additional information
-
downregulation of HtrA1 by siRNA increases osteoblast mineralization, HtrA1 overexpression inhibits osteoblast mineral deposition in vitro and prevents BMP-2-induced mineralization, overview
additional information
-
a htrA mutant, consisting of a truncated gene, demonstrates no significant difference to the W83 parent strain when subjected to high temperature and pH values from 3 to 11. Mutant shows increased sensitivity to H2O2. HtrA mutant shows increased invasion in KB cells and gingival epithelial cells. Microarray experiments indicate that a total of 253 genes are differentially regulated in the htrA mutant, including a group of stress-related genes. In animal experiments, a competition assay shows that the htrA mutant does not survive as well as the wild-type. Fewer mice infected with the htrA mutant die than mice infected with W83, suggesting that the htrA gene is virulence-related
additional information
-
the respiratory defect of Rhodobacter capsulatus DsbA-null mutants originates from the overproduction of the periplasmic protease DegP, which renders them temperature sensitive for growth, the mutants are viable and proficient in photosynthesis, phenotype, overview, overproduction of DegP abolishes the newly restored respiratory growth ability of the revertants in all growth media
additional information
-
disruption of the YNM3 gene encoding Ynm3p results in increased fatty acid uptake, triglyceride accumulation, and reduced expression of the fatty acid-responsive OLE1 gene encoding the essential DELTA9-acyl-CoA desaturase, as well as in increased Faa1p and Faa4p ACSL activities, strain-specific phenotypes associated with deletion of YNM3 include the inability to grow on non-fermentable carbon sources and altered cellular morphology, overview
additional information
construction of deg deletion mutants by allelic replacement, the viability of degP and degS deletion mutants, as well as of the double mutant DELTAdegP-degS, is affected at elevated temperatures, while the degQ deletion mutant viability is not, also the triple mutant degP-degS-degQ is not severely affected, transfection of the mutants in the mouse-virulent strain SL1344 and injection into BALB/c mice, which leads attenuation of degP, degS, double, and triple mutants, while the degQ mutant is virulent as the wild-type strain, several maturally occuring mutations in the deg genes influence the virulence and pathology of Salmonella typhimurium strains, overview
additional information
-
mutant P1149 containing an insertion deletion mutation in the caiRH locus resulting in substantially lower HtrA expression
additional information
-
mutant HTR10 with in-frame deletion in htrA, mutant HTR10 is unable to efficiently process the protease proprotein, shows a dramatic increase in hemolytic activity and is virulent in a murine model of subcutaneous infection, mutant HSC11 with a deletion control strain that is a sibling of mutant HTR10 but contains wild-type htrA
additional information
-
construction of the deletion mutant HSC5DELTAhtrA
additional information
-
deletion of the PDZ domain severely impairs proteolytic activity
additional information
HtrA knockdown promotes anterior development and impairs mesoderm and neuronal differentiation, phenotype, overview
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