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2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
?
2-[2-(2-[[4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoyl]amino]ethoxy)ethoxy]ethanaminium + H2O
?
-
-
-
-
?
4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoic acid + H2O
?
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl benzoate + H2O
4-nitrophenol + benzoate
-
-
-
?
4-[2-(2-[[4-([1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino)-4-oxobutanoyl]amino]ethoxy)ethoxy]butanoate + H2O
?
-
-
-
-
?
acetyl-L-Leu methyl ester + H2O
acetyl-L-Leu + methanol
-
-
-
-
?
acetyl-L-Trp ethyl ester + H2O
acetyl-L-Trp + ethanol
-
-
-
-
?
acetyl-L-Tyr ethyl ester + H2O
acetyl-L-Tyr + ethanol
-
-
-
-
?
acetyl-Leu methyl ester + H2O
acetyl-Leu + methanol
-
-
-
-
?
acetyl-Phe ethyl ester + H2O
acetyl-Phe + ethanol
-
-
-
-
?
acetyl-Trp ethyl ester + H2O
acetyl-Trp + ethanol
-
-
-
-
?
acetyl-Tyr ethyl ester + H2O
acetyl-Tyr + ethanol
-
-
-
-
?
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
?
-
-
-
-
?
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
?
azocasein + H2O
?
-
-
-
-
?
azocasein + H2O
fragments of azocasein
benzoyl-4-guanidinophenyl ester + H2O
benzoyl + 4-guanidinophenol
-
-
-
?
benzoyl-D-Ala-4-guanidinophenyl ester + H2O
benzoyl-D-Ala + 4-guanidinophenol
-
5% of activity with N-benzoyl-L-Ala-4-guanidinophenyl ester
-
?
benzoyl-D-Leu-4-guanidinophenyl ester + H2O
benzoyl-D-Leu + 4-guanidinophenol
-
-
-
?
benzoyl-D-Phe-4-guanidinophenyl ester + H2O
benzoyl-D-Phe + 4-guanidinophenol
-
-
-
?
benzoyl-Gly-4-guanidinophenyl ester + H2O
benzoyl-Gly + 4-guanidinophenol
-
-
-
?
benzoyl-L-Ala-4-guanidinophenyl ester + H2O
benzoyl-L-Ala + 4-guanidinophenol
-
-
-
?
benzoyl-L-tyrosine ethyl ester + H2O
?
-
-
-
-
?
benzoyl-L-tyrosine ethyl ester + H2O
benzoyl-L-tyrosine + ethanol
-
-
-
-
?
benzoyl-Tyr-p-nitroanilide + H2O
benzoyl-tyrosine + p-nitroaniline
-
-
-
?
benzoyl-tyrosine p-nitroanilide + H2O
benzoyl-tyrosine + p-nitroaniline
-
-
-
?
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu + 4-nitroaniline
-
-
-
-
?
beta-lactoglobulin A + H2O
?
-
-
chymotrypsin hydrolyzes beta-lactoglobulin A after Tyr, Trp, Phe, Met, and Leu, peptides corresponding to the cleavage of bonds with Glu, Gln, and Lys at the N-terminal side are also found
-
?
BH3 interacting domain death agonist + H2O
?
-
-
-
?
bovine chymotrypsinogen + H2O
?
-
-
-
-
?
Bovine serum albumin + H2O
?
bovine serum albumine + H2O
?
-
-
-
-
?
calcineurin + H2O
?
-
-
chymotrypsin cleaves calcineurin into a 47-kDa fragment and a 13-kDa fragment, the cleavage activates the phosphatase activity of calcineurin in a Ca2+/CaM-independent manner
-
?
casein + H2O
fragments of casein
cationic trypsinogen + H2O
?
-
-
-
-
?
cheese whey protein + H2O
?
-
-
-
-
r
Collagen + H2O
?
-
-
-
-
?
deltamethrin + H2O
3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
-
-
-
?
Gelatin + H2O
?
-
chymotrypsin A is active in degrading gelatin, while chymotrypsin B does not reveal activity against gelatin
-
-
?
hippuryl-L-phenylalanine + H2O
hippuric acid + L-phenylalanine
-
1 mM in 25mM Tris-HCl buffer containing 0.5M NaCl
-
-
?
L-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
L-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
?
L-Tyr ethyl ester + H2O
L-Tyr + ethanol
m-calpain + H2O
fragments of m-calpain
-
-
-
?
mCry3A + H2O
mCry3A fragments
-
Bacillus thuringiensis toxin
chymotrypsin cleaves mCry3A into stable 55000 and 49000 Da fragments, as well as smaller polypeptides having molecular masses of 6000 to 11000 Da
-
?
milk-acetate mixture + H2O
?
-
-
-
-
?
mu-calpain + H2O
fragments of mu-calpain
-
-
-
?
muscle collagen + H2O
?
-
moderate in vitro activity using muscle collagen as a substrate
-
-
?
N-acetyl-DL-Phe methyl ester + H2O
N-acetyl-L-Phe + methanol
-
alpha-chymotrypsin immobilized to mesoporous silica
enatiomeric excess greater than 99%
?
N-acetyl-DL-Phe-beta-naphthylester + H2O
N-acetyl-DL-Phe + beta-naphthol
-
-
-
?
N-acetyl-DL-phenylglycine methyl ester + H2O
N-acetyl-L-phenylglycine + methanol
-
alpha-chymotrypsin immobilized to mesoporous silica
enatiomeric excess greater than 99%
?
N-acetyl-L-leucine methyl ester + H2O
N-acetyl-L-leucine + methanol
N-acetyl-L-Phe methyl ester + H2O
N-acetyl-L-Phe + methanol
-
alpha-chymotrypsin immobilized to mesoporous silica
-
?
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Pal + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOH)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOH) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Phe + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid + H2O
N-acetyl-L-Phe-Ala-Thr-Tyr + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-acetyl-L-phenylalanine ethyl ester + glycinamide
N-Ac-Phe-Gly-NH2 + ethanol
-
-
-
-
?
N-acetyl-L-phenylalanine ethyl ester + H2O
N-acetyl-L-phenylalanine + ethanol
N-acetyl-L-tryptophan ethyl ester + H2O
N-acetyl-L-tryptophan + ethanol
N-acetyl-L-Tyr ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
-
-
?
N-acetyl-L-Tyr-ethyl ester + H2O
N-acetyl-L-Tyr + ethanol
-
-
-
?
N-acetyl-L-Tyr-p-nitroanilide + H2O
N-acetyl-L-Tyr + p-nitroaniline
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
N-alpha-acetyl-L-tyrosine ethyl ester + H2O
N-alpha-acetyl-L-tyrosine + ethanol
-
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
? + p-nitroaniline
N-alpha-benzoyl-L-tyrosine ethyl ester + H2O
?
-
-
-
-
?
N-alpha-benzoyl-L-tyrosyl ethyl ester + H2O
?
-
-
-
-
?
N-benzoyl-L-arginine ethyl ester + H2O
N-benzoyl-L-arginine + ethanol
-
0.5 mM in 50 mM phosphate buffer, pH 7.6
-
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
N-benzoyl-L-Tyr + ethanol
N-benzoyl-L-Tyr p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-tyrosine + 4-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine 4-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
?
-
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
N-benzoyl-L-tyrosine ethyl ester + methanol
N-benzoyl-L-tyrosine methyl ester + ethanol
-
transesteriferication
-
?
N-benzoyl-L-tyrosine methyl ester + ethanol
N-benzoyl-L-tyrosine ethyl ester + methanol
-
transesteriferication
-
?
N-benzoyl-L-tyrosine methyl ester + H2O
N-benzoyl-L-tyrosine + methanol
-
-
-
?
N-benzoyl-L-tyrosine p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine-4-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
N-benzoyl-L-tyrosyl-ethyl ester + H2O
?
-
-
-
?
N-benzoyl-Tyr p-nitroanilide + H2O
N-benzoyl-Tyr + p-nitroaniline
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid + H2O
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2) + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid + H2O
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr + 5-amino-2-nitrobenzoic acid
-
-
-
-
?
N-glutamyl-L-phenylalanine p-nitroanilide + H2O
p-nitroaniline + N-glutamyl-L-phenylalanine
-
-
-
-
?
N-glutaryl-L-Phe-p-nitroanilide + H2O
N-glutaryl-L-Phe + p-nitroaniline
-
-
-
?
N-glutaryl-L-phenylalanine-4-nitroanilide + H2O
N-glutaryl-L-phenylalanine + 4-nitroaniline
-
-
-
?
N-glutaryl-L-phenylalanine-p-nitroanilide + H2O
N-glutaryl-L-phenylalanine + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Ala p-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Arg + p-nitroaniline
-
very low activity
-
?
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Lys + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Lys + p-nitroaniline
-
extremly low activity
-
?
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Met + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Nle + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Nva + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
N-succinyl-Ala-Phe-Ala + H2O
N-succinyl-Ala-Phe + alanine
-
-
-
?
N-succinyl-Ala-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Gly-Gly-4-nitroanilide + H2O
N-succinyl-Gly-Gly + 4-nitroaniline
-
-
-
?
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Pro + p-nitroaniline
-
-
-
-
?
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-L-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine p-nitroanilide + H2O
p-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine
-
-
-
-
?
N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-p-nitroanilide + H2O
p-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine
-
-
-
-
?
N-succinyl-L-Phe-p-nitroanilide + H2O
N-succinyl-L-Phe + p-nitroaniline
N-succinyl-L-phenylalanine p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
-
-
-
-
?
N-succinyl-L-phenylalanine-4-nitroanilide + H2O
N-succinyl-L-phenylalanine + 4-nitroaniline
-
-
-
?
N-succinyl-L-phenylalanine-p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Phe + 7-amino-4-methylcoumarin
N-succinyl-Phe-p-nitroanilide + H2O
p-nitroaniline + N-succinyl-Phe
-
-
-
-
?
N-[1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl]-N'-[2-[2-(2-hydroxyethoxy)ethoxy]ethyl]butanediamide + H2O
?
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Ala + p-nitrophenol
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Leu p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Leu + p-nitrophenol
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Lys + p-nitrophenol
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Tyr + p-nitrophenol
-
-
-
-
?
Nalpha-tosyl-L-Tyr benzyl ester + H2O
Nalpha-tosyl-L-Tyr + phenol
-
-
-
-
?
native collagen + H2O
?
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
rat chymotrypsinogen + H2O
?
-
-
-
-
?
SPPNA + H2O
?
-
specific substrate for chymotrypsin
-
-
?
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
Suc-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
succinimidyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinimidyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
42% of activity with succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
?
succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Met + p-nitroaniline
66% of activity with succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
?
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
-
57 microM, pH 7.8
-
-
?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Tyr + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-Gly-Gly-p-nitroanilide + H2O
succinyl-Gly-Gly + p-nitroaniline
-
-
-
-
?
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide + H2O
succinyl-L-Ala-Ala-Pro-L-Phe + 4-nitroaniline
-
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Leu + 4-nitroaniline
-
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Phe + p-nitroaniline
-
-
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
Trypsinogen + H2O
?
-
-
-
-
?
Z-Phe-Arg-Thr-Tyr-NH-(3-carbamoyl-4-nitrophenol) + H2O
Z-Phe-Arg-Thr-Tyr + 5-amino-2-nitrobenzamide
-
-
-
-
?
additional information
?
-
azocasein + H2O
fragments of azocasein
-
-
-
?
azocasein + H2O
fragments of azocasein
-
-
-
?
Bovine serum albumin + H2O
?
-
-
-
?
Bovine serum albumin + H2O
?
-
-
-
-
?
Bovine serum albumin + H2O
?
-
partial hydrolysis
-
?
casein + H2O
?
-
-
-
-
?
casein + H2O
fragments of casein
-
-
-
?
casein + H2O
fragments of casein
-
-
-
?
casein + H2O
fragments of casein
-
-
-
?
casein + H2O
fragments of casein
-
-
?
L-Tyr ethyl ester + H2O
L-Tyr + ethanol
-
-
-
-
?
L-Tyr ethyl ester + H2O
L-Tyr + ethanol
-
-
-
-
?
N-acetyl-L-leucine methyl ester + H2O
N-acetyl-L-leucine + methanol
-
5% of kcat with N-acetyl-L-tyrosine ethyl ester
-
?
N-acetyl-L-leucine methyl ester + H2O
N-acetyl-L-leucine + methanol
-
5% of kcat with N-acetyl-L-tyrosine ethyl ester
-
?
N-acetyl-L-phenylalanine ethyl ester + H2O
N-acetyl-L-phenylalanine + ethanol
-
-
-
?
N-acetyl-L-phenylalanine ethyl ester + H2O
N-acetyl-L-phenylalanine + ethanol
-
-
-
?
N-acetyl-L-tryptophan ethyl ester + H2O
N-acetyl-L-tryptophan + ethanol
-
-
-
?
N-acetyl-L-tryptophan ethyl ester + H2O
N-acetyl-L-tryptophan + ethanol
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
-
-
-
?
N-acetyl-L-tyrosine ethyl ester + H2O
N-acetyl-L-tyrosine + ethanol
-
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
? + p-nitroaniline
-
BApNA
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
? + p-nitroaniline
-
BApNA
-
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
N-benzoyl-L-Tyr + ethanol
-
-
-
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
N-benzoyl-L-Tyr + ethanol
-
alpha-chymotrypsin immobilized to mesoporous silica
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
N-benzoyl-L-Tyr + ethanol
-
-
-
-
?
N-benzoyl-L-Tyr ethyl ester + H2O
N-benzoyl-L-Tyr + ethanol
-
-
-
-
?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
-
?
N-benzoyl-L-tyrosine ethyl ester + H2O
N-benzoyl-L-tyrosine + ethanol
-
-
-
?
N-benzoyl-L-tyrosine-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
-
-
-
?
N-benzoyl-L-tyrosine-p-nitroanilide + H2O
N-benzoyl-L-tyrosine + p-nitroaniline
-
-
-
-
?
N-benzoyl-Tyr p-nitroanilide + H2O
N-benzoyl-Tyr + p-nitroaniline
-
-
-
-
?
N-benzoyl-Tyr p-nitroanilide + H2O
N-benzoyl-Tyr + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
preferred substrate
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
SucAAPFpNA
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
SucAAPFpNA
-
-
?
N-succinyl-L-Phe-p-nitroanilide + H2O
N-succinyl-L-Phe + p-nitroaniline
-
-
-
?
N-succinyl-L-Phe-p-nitroanilide + H2O
N-succinyl-L-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-L-Phe-p-nitroanilide + H2O
N-succinyl-L-Phe + p-nitroaniline
-
-
-
-
?
N-succinyl-L-phenylalanine-p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
-
-
-
-
?
N-succinyl-L-phenylalanine-p-nitroanilide + H2O
N-succinyl-L-phenylalanine + p-nitroaniline
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Phe + 7-amino-4-methylcoumarin
-
-
-
?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Phe + 7-amino-4-methylcoumarin
-
-
-
?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
N-succinyl-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
Protein + H2O
?
-
enzyme form LCTb plays an important role in establishing the myiasis-causing larvae of Lucilia cuprina on host skin as well as providing nutrients for the rapidly growing larvae
-
-
?
Protein + H2O
?
-
catalyzes the hydrolysis of peptide bonds of protein foods in mammalian gut
-
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
-
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
chymotrypsin A shows much lower catalytic efficiency to the substrate than chymotrypsin B
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
additional information
?
-
-
chymotrypsin cleaves a number of detectable membrane proteins of bovine and equine erythrocytes (at least 87000 Da, 71000 Da and 33000 Da fragments)
-
-
?
additional information
?
-
a variety of experimental methods, including fluorescence emission, dynamic quenching, steady-state fluorescence anisotropy, far-UV circular dichroism, nuclear magnetic resonance spectroscopy, and dynamic light scattering are employed to characterize the conformational states of achymotrypsin that precede formation of amyloid fibrils
-
-
?
additional information
?
-
-
specificity of Chtr1 reactive site is determined by hydrolysis of the oxidized bovine insulin B-chain
-
-
?
additional information
?
-
-
chymotrypsin cleaves peptides at the carboxyl side of aromatic rings. Niosomal encapsulation by nonionic surfactant based vesicles protects the antineoplastic agent Paclitaxel from chymotrypsin degradation
-
-
?
additional information
?
-
-
chymotrypsin does not reveal activity against gelatin
-
-
?
additional information
?
-
-
mutant Y50G of the wheat subtilisin/chymotrypsin inhibitor acts as a common substrate for chymotrypsin
-
-
?
additional information
?
-
-
N-alpha-benzoyl-DL-arginine p-nitroanilide synthetic substrate
-
-
?
additional information
?
-
-
does not cleave the Bacillus thuringiensis toxin Cry3A
-
-
?
additional information
?
-
-
specific hydrolysis of peptide bonds on the carboxyl side of Tyr, Phe, Trp, His, Leu Thr and Gly
-
-
?
additional information
?
-
-
primary specificity (kcat/Km) for Phe rather than Tyr and Leu residues. No substrates: short substrates such as N-succinyl-Phe-7-amido-4-methylcoumarin
-
-
?
additional information
?
-
-
no activity with benzoyl-Arg-p-nitroanilide
-
-
?
additional information
?
-
the protease exhibits activity toward chymotrypsin and trypsin substrates. Substrate specificity to a variety of 7-amino-4-methylcoumarin-linked peptides in the order of decreasing activity: AAF, AFK, IIW, LY, GGF, AAPV. Little or no activity is detected on FVR, LLVY or GGR. No activity is noted on casein
-
-
?
additional information
?
-
-
the protease exhibits activity toward chymotrypsin and trypsin substrates. Substrate specificity to a variety of 7-amino-4-methylcoumarin-linked peptides in the order of decreasing activity: AAF, AFK, IIW, LY, GGF, AAPV. Little or no activity is detected on FVR, LLVY or GGR. No activity is noted on casein
-
-
?
additional information
?
-
the protease exhibits activity toward chymotrypsin and trypsin substrates. Substrate specificity to a variety of 7-amino-4-methylcoumarin-linked peptides in the order of decreasing activity: AAF, AFK, IIW, LY, GGF, AAPV. Little or no activity is detected on FVR, LLVY or GGR. No activity is noted on casein
-
-
?
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(Cbz-alanyl)aminomethyl boronic acid
-
inhibition decreases in the presence of arabinogalactan
(Cbz-phenylalanyl)aminomethyl boronic acid
-
inhibition decreases in the presence of arabinogalctan
(methanethiolato)[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium (V)
-
-
(p-methoxyphenylthiolato-S)[2,6-bis[(mercapto-kappaS)methyl]pyridine-kappaN] oxorhenium(V)
-
-
(S)-(1-benzyl-2-thiolethyl)-carbamic acid benzyl ester
-
inhibition of alpha-chymotrypsin in the presence of Zn2+
1,2-propanediol
residual activity of bovine pancreatic alpha-chymotrypsin is studied in water/1,2-propanediol mixtures in the entire range of water content at 25°C. The degree of stabilization/ destabilization of alpha-chymotrypsin depends strongly on the water content in organic solvent. At high water content, the residual activity values are higher than 100%. At low water content, the residual catalytic activity is about 90-95%, compared with that observed after incubation in pure water
2,2'-[[(1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphoryl]bis(oxybenzene-4,1-diyl)]diacetic acid
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
2,2'-[[(1-[[1-(tert-butoxycarbonyl)-L-prolyl]amino]-3-methylbutyl)phosphoryl]bis(oxybenzene-3,1-diyl)]diacetic acid
-
-
2,3-dichlorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
2,5-dichlorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
2-(2,6-difluorophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(2,6-dimethoxyphenyl)-4H-3,1-benzoxazin-4-one
mixed-type inhibition
2-(2-bromophenyl)-4H-3,1-benzoxazin-4-one
competitive inhibition
2-(2-fluorophenyl)-4H-3,1-benzoxazin-4-one
mixed-type inhibition
2-(2-methoxyphenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(2-methylphenyl)-4H-3,1-benzoxazin-4-one
competitive inhibition
2-(2-nitrophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(2-thienyl)-4H-3,1-benzoxazin-4-one
competitive inhibition
2-(3,4,5-trimethoxyphenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(3-bromophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(3-bromophenyl)-7-nitro-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(3-chlorophenyl)-4H-3,1-benzoxazin-4-one
mixed-type inhibition
2-(3-chlorophenyl)-7-methyl-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(3-fluorophenyl)-4H-3,1-benzoxazin-4-one
mixed-type inhibition
2-(3-methylphenyl)-4H-3,1-benzoxazin-4-one
competitive inhibition
2-(3-methylphenyl)-7-nitro-4H-3,1-benzoxazin-4-one
competitive inhibition
2-(3-nitrophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(4-bromophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(4-fluorophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(4-methoxyphenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-(4-methylphenyl)-4H-3,1-benzoxazin-4-one
mixed-type inhibition
2-(4-nitrophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
2-hydroxymethyl-1-(2-phenylethanecarbonyl)pyrrole
-
0.0125 mg/ml, 10% inhibition
2-hydroxymethyl-1-(2-phenylethansulfonyl)pyrrole
-
0.0125 mg/ml, 15% inhibition
2-hydroxymethyl-1-(2-phenylethenylsulfonyl)pyrrole
-
0.0125 mg/ml, 15% inhibition
2-hydroxymethyl-1-(N-phthalyl-L-leucine)pyrrole
-
0.0125 mg/ml, 10% inhibition
2-hydroxymethyl-1-(phenylsulfonyl)pyrrole
-
0.053 mM, 20% inhibition
2-nitrophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
2-oxo-2-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
enediyne-amino acid conjugate, competitive inhibitor
2-oxo-2-(piperidin-1-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
piperidine amino acid conjugate, exhibits lesser inhibitory activity compared to the enediyne-peptide conjugates
2-oxo-2-[(5Z)-3,4,7,8-tetradehydro-9,10-dihydroazecin-1(2H)-yl]ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
enediyne-amino acid conjugate
2-phenyl-4H-3,1-benzoxazin-4-one
mixed-type inhibition
3,4-dichloroisocoumarin
complete inhibition at 0.2 mM
3,5-dichlorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-bromophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-chloro-5-methoxyphenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-chlorophenyl-6(acetoxymethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-chlorophenyl-6-methyl-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-fluorophenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-methoxyphenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
3-trifluoromethylphenyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
4-fluorophenylboronic acid
-
-
4-methyl-N-[2-(4-oxo-4H-3,1-benzoxazin-2-yl)phenyl]benzenesulfonamide
non-competitive inhibition
4-[(4-nitrophenyl)sulfonyl]-1,2,7,8-tetradehydro-3,4,5,6-tetrahydro-4-benzazecine
-
5'-demethoxyepiexcelsin
-
from Commiphora mukul, noncompetitive inhibitor
7-chloro-2-(2-fluorophenyl)-4H-3,1-benzoxazin-4-one
mixed-type inhibition
7-chloro-2-(2-methylphenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
7-chloro-2-(4-nitrophenyl)-4H-3,1-benzoxazin-4-one
non-competitive inhibition
7-chloro-2-phenyl-4H-3,1-benzoxazin-4-one
non-competitive inhibition
7-hydroxycoumarin
0.78 mM, 50% inhibition
Ac-Phe-Val-Thr-p-amino-L-phenylalanine-CHO
-
-
Ac-Phe-Val-Thr-p-nitro-L-phenylalanine-CHO
-
-
Ac-Phe-Val-Thr-Phe-CHO
-
-
Ac-Phe-Val-Thr-pyridyl-L-alanine-CHO
-
-
Ac-Phe-Val-Thr-Tyr-CHO
-
-
Acacia confusa chymotrypsin inhibitor
-
large, dimeric 70 kDa chymotrypsin inhibitor with a molar ratio of inhibition between the chymotrypsin inhibitor and alpha-chymotrypsin of 2:1, which gives about 100% inhibition. The molar ratio of chymotrypsin inhibitor to chymotrypsin is usually 1:1. Its chymotrypsin inhibitory activity is stable from pH 3-10 and from 0-50°C. Exerts antiproliferative activity toward breast cancer MCF-7 cells
-
Acacia plumosa trypsin inhibitor A
-
strong inhibition
-
Acacia plumosa trypsin inhibitor B
-
strong inhibition
-
Acacia plumosa trypsin inhibitor C
-
strong inhibition
-
acetone
-
10-50%, significantly reduces residual activity to about 80 % at all tested concentrations
aceto[2,6-bis[(butylthio-jS)methyl]phenyl-kappaC]-,(SP-4-3)-palladium(II)
-
-
alpha-chymostatin
-
specific inhibitor
alpha1-Aantichymotrypsin
-
forms very long-lived, enzymatically inactive 1/1 compexes with its target
-
alpha2-Macroglobulin
-
nonspecific proteinase inhibitor
-
Alzheimer's amyloid beta-protein precursor
-
engineering of inhibitors with altered specificities
-
anti-trypsin-chymotrypsin A
-
highly potent serine protease inhibitor protein from Theromyzon tessulatum
-
anti-trypsin-chymotrypsin B
-
highly potent serine protease inhibitor protein from Theromyzon tessulatum
-
basic pancreatic trypsin inhibitor
-
Belactosin C
-
irreversible inhibitor
benzoxazinone
the presence of substituents on benzene ring reduces the inhibitory potential of benzoxazinone. The increased inhibitory potential due to fluoro group at phenyl substituent is observed followed by chloro and bromo substituents. Compounds with strong electron donating or withdrawing groups on phenyl substituent, show a good inhibitory potential at ortho > meta > para position. Kinetic studies show diverse types of inhibition, except uncompetitive-type inhibition
benzyloxycarbonyl-Ala-Ala-Phe-glyoxal
-
-
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
benzyloxycarbonyl-Phe-glyoxal
-
-
benzyloxycarbonyl-Pro-Phe-glyoxal
-
-
BGTI1
-
concentration 228 nM: 100% inhibition, concentration 456 nM: 97.8% inhibition, concentration 114 nM: 73.3% inhibition, concentration 57 nM: 41.3% inhibition. Inhibitor from Vigna mungo seeds
-
BGTI2
-
concentration 2188 nM: 53.3% inhibition, concentration 1094 nM: 28.9% inhibition, concentration 547 nM: 11.1% inhibition, concentration 273 nM: 6.7% inhibition. Inhibitor from Vigna mungo seeds
-
BGTI3
-
concentration 5000 nM: 36.7% inhibition, concentration 2500 nM: 22.3% inhibition, concentration 1250 nM: 10.0% inhibition, concentration 625 nM: 0.0% inhibition. Inhibitor from Vigna mungo seeds
-
bis(2,3-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(2-methylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(3,4,5-trimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
bis(3,4-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
bis(3-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
bis(4-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(4-methoxyphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis(4-tert-butylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis[4-(propan-2-yl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
one of the most potent inhibitors of chymotrypsin within phosphonic analogs of phenylalanine
bis[4-(sulfanylmethyl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
black-eyed pea trypsin/chymotrypsin inhibitor
BTCI, is able to inhibit trypsin and chymotrypsin simultaneously by forming a stable ternary complex
-
bovine basic panreatic trypsin inhibitor
-
-
-
Bovine pancreatic trypsin inhibitor
-
300fold stronger inhibition of alpha-chymotrypsin in the presence of 3 M NaCl
-
Bowman-Birk inhibitor
-
-
BPTI
-
basic pancreatic trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected
-
brein 3-O-myristate
-
0.000078 mM, 50% inhibition, noncompetitive inhibition
brein 3-O-palmitate
-
0.000042 mM, 50% inhibition, noncompetitive inhibition
broad bean trypsin-chymotrypsin inhibitor
-
7500 Da peptide isolated from from Vicia faba, almost complete inhibition at a molar ratio of 20
-
Cd2+
-
partially inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 76.8% and 49.7% residual activity for chymotrypsin A and B, respectively
chicken ovomucoid inhibitor
-
-
-
chloro[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium(V)
-
-
CMTI I
-
Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect
-
CMTI IV
-
Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect
-
complex of vanadate and benzohydroxamic acid
competitive inhibitor of alpha-chymotrypsin, consisting of 1 mM vanadate and 2 mM benzohydroxamic acid
CPGT I
-
Cucurbita pepo var. giramontia inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed
-
CPTI IV
-
Cucurbita pepo inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed
-
cyanopeptolin
Arg-containing cyanopeptolins inhibit trypsin at low IC50 values (0.00024-0.00026 mM)and show mild activity against chymotrypsin (IC50 0.0031-0.0038 mM), while Tyr-containing cyanopeptolins are selectively and potently active against chymotrypsin (IC50 0.00026 mM). Neither of the cyanopeptolins are active against thrombin, elastase or protein phosphatase 1. Two cyanopeptolins (CP962 and CP985) have no cytotoxic effects on MCF-7 breast cancer cells. Strong and selective activity of the new cyanopeptolin variants makes them potential candidates for the development of drugs against metabolic disorders and other diseases
-
cyanopeptolin 1020
isolated from Nostoc edaphicum CCNP1411
cyanopeptolin 1027
isolated from Nostoc edaphicum CCNP1411
cyanopeptolin 978
isolated from Nostoc edaphicum CCNP1411
cyanopeptolin 985
isolated from Nostoc edaphicum CCNP1411
cyanopeptolin 992
isolated from Nostoc edaphicum CCNP1411
cycloart-24-ene-3beta-ol
-
0.00014 mM, 50% inhibition, noncompetitive inhibition
cyclododecyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
cyclohexyl-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carboxylate
-
-
cyclo[-Gly-Arg-cyclo[-S-Cys-Thr-Lys-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp-]
-
sunflower trypsin inhibitor, SFTI-1
CyPTI VII
-
trypsin inhibitors from Cyclanthera pedata, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed
-
dammara-20,24-dien-3beta-ol
-
0.00013 mM, 50% inhibition, competitive inhibition
diphenyl (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
-
DMSO
-
10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45%
epiexcelsin
-
from Commiphora mukul, noncompetitive inhibitor
faradiol 3-O-myristate
-
0.000032 mM, 50% inhibition, noncompetitive inhibition
faradiol 3-O-palmitate
-
0.000072 mM, 50% inhibition, noncompetitive inhibition
Gly-Arg-cyclo[-S-Cys-Thr-Pal-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-CH3)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-F)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-guanidine)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-NH2)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Phe(p-NO2)-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
Gly-Arg-cyclo[-S-Cys-Thr-Tyr-Ser-Ile-Pro-Pro-Ile-Cys-S-]-Phe-Pro-Asp
-
-
IVNGEEAVPGSWPW
-
autocatalytically produced 14-residue fragment from a three-phase-partitioning-treated chymotrypsin
Leu-Phe-p-fluorophenylmethylamide
-
strong inhibition
Lima bean trypsin inhibitor
-
lup-20(29)-ene-3beta,16beta-diol
-
0.00012 mM, 50% inhibition, competitive inhibition
LUTI
-
Linum usitatissimum inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect
-
maniladiol 3-O-myristate
-
0.000078 mM, 50% inhibition, competitive inhibition
maniladiol 3-O-palmitate
-
0.000084 mM, 50% inhibition, noncompetitive inhibition
methyl-4-hydroxy-4-[1-(phenylsulfonyl)pyrrol-2-yl]butanoate
-
0.0125 mg/ml, 10% inhibition
MG-132
-
reversible inhibitor
micropeptin 478-A
-
potent inhibitor
micropeptin 478-B
-
moderate inhibitor
micropeptin HU1021
-
weak inhibitor
micropeptin HU1041
-
weak inhibitor
micropeptin HU1069
-
weak inhibitor
micropeptin HU895A
-
moderate inhibitor
micropeptin HU895B
-
potent inhibitor
micropeptin HU909
-
potent inhibitor
micropeptin HU975
-
moderate inhibitor
micropeptin HU989
-
moderate inhibitor
mixed monolayer protected gold cluster
-
anionically functionalized amphiphilic nanoparticles
-
Mn2+
-
partially inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 85.3% and 48.9% residual activity for chymotrypsin A and B, respectively
N-(2-acetylphenyl)-N'-(3-methylphenyl) urea
-
N-(2-methylphenyl)-2-oxo-1-pyrrolidine carboxamide
most active inhibitor
N-(tert-butoxycarbonyl)-L-alanyl-N-[4-[2-(3-oxoprop-1-yn-1-yl)phenyl]but-3-yn-1-yl]-L-phenylalaninamide
enediyne-amino acid conjugate
N-(tert-butoxycarbonyl)-L-alanyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-alanyl-N6-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N-(naphthalen-2-ylmethyl)-6-oxo-L-lysinamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-leucyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-phenylalanyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-(diphenoxyphosphoryl)-2-phenylethyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-tert-butylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
tripeptide containing leucine-related phosphonates, which is a relatively weak chymotrypsin inhibitor
N-(tert-butoxycarbonyl)glycyl-N1-benzyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-L-glutamamide
-
irreversible inhibitor, significant inhibition of 20S proteasome chymotrypsin-like (beta5) activity, inhibitory potency is similar to that of Belactosin C
N-(tert-butoxycarbonyl)glycyl-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor, possesses the greatest inhibition of the chymotrypsin-like activity, inhibitory potency is similar to that of Belactosin C. Increases the G2/M cell distribution in a similar manner to that of PS-341, and induces apoptosis at more than 0.001 mM concentration of compound in HeLa cells
N-(tert-butoxycarbonyl)glycyl-N6-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N-(naphthalen-2-ylmethyl)-6-oxo-L-lysinamide
-
irreversible inhibitor
N-acetyl-L-Phe-Ala-Thr-Pal aldehyde
-
-
N-acetyl-L-Phe-Ala-Thr-Phe aldehyde
-
-
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
-
N-acetyl-L-Phe-Ala-Thr-Tyr aldehyde
-
-
N-alpha-tosyl-L-Phe chloromethyl ketone
N-benzoyl-L-tyrosine ethyl ester
-
-
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone
N-p-tosyl-L-phenylalanine-chloromethyl ketone
complete inhibition at 5 mM
N-succinyl-L-phenylalanine
-
-
N-toluenesulfonyl-L-phenylalanine chloromethyl-ketone
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, partially inhibits (53% residual activity)
N-toluenesulfonyl-L-phenylalanine chloromethylketone
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, partially inhibits chymotrypsin I (56% residual activity) and chymotrypsin II (60% residual activity)
N-toluenesulfonyl-L-phenylalanine-chloromethyl ketone
-
56% residual activity at 1 mg/ml
N-tosyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
-
N-tosyl-L-phenylalanine chloromethyl ketone
-
significant inhibition, especially to chymotrypsin B. At room temperature, at 0.05 mM concentration, 35.6% and 17.5% residual activity for chymotrypsin A and B, respectively
N-tosyl-L-phenylalanine-chloromethyl ketone
-
chymotrypsin inhibitor TPCK
N-tosyl-L-phenylalanyl chloromethyl ketone
-
-
N2-(tert-butoxycarbonyl)-N4-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-aspartamide
-
irreversible inhibitor
N2-(tert-butoxycarbonyl)-N5-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N1-(naphthalen-2-ylmethyl)-L-glutamamide
-
irreversible inhibitor
N2-(tert-butoxycarbonyl)-N6-[(1R)-1-(dihydroxyboranyl)-3-methylbutyl]-N-(naphthalen-2-ylmethyl)-6-oxo-L-lysinamide
-
irreversible inhibitor
NaCl
-
continuous decrease in activity with increasing NaCl concentration, showing 95, 91, 85, 74, 68, and 63% residual activity at 5, 10, 15, 20, 25, and 30% of NaCl, respectively
p-nitrophenyl-p'-guanidino-benzoate
-
NPGB
p-octyloxybenzyltributylammonium bromide
-
5 mM, approx. 90% inactivation after 24 h
p-octyloxybenzyltrimethylammonium bromide
-
10 mM, complete inactivation
p-tosyl-L-phenylalanine chloromethane
-
-
P1 bovine chymotrypsin-bovine pancreatic trypsin inhibitor
-
P1 BPTI, P1-Xaa variants
-
pars intercerebralis major peptide
-
i.e. PMP-C, potent alpha-chymotrypsin inhibitor protein from the insect Locusta migratoria
-
Pefabloc SC
-
effectively inhibits both chymotrypsin A and B. At room temperature, at 1 mM concentration, 1.2% and 1.1% residual activity for chymotrypsin A and B, respectively
pepstatin
-
shows minimal inhibitory effect on both chymotrypsin A and B. At room temperature, at 0.03 mM concentration, 95.9% and 92.3% residual activity for chymotrypsin A and B, respectively
phenyl methyl sulfonyl fluoride
-
-
phenylmethanesulfonyl fluoride
-
effectively inhibits both chymotrypsin A and B. At room temperature, at 1 mM concentration, 0.4% and 0.3% residual activity for chymotrypsin A and B, respectively
phenylmethylsulfonyl fluoride
phenylmethylsulfonyl-fluoride
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits (4% residual activity)
Phenylmethylsulfonylfluoride
photolytic 1-(2-nitrophenyl)ethanol residues
-
1-(2-nitrophenyl)ethanol coated protein can be reactivated by 15 min UV-A radiation
-
Propanol
-
reduces the residual activity to about 80 % when tested at concentrations of 10 and 30%. Activity is not affected using 50%
PS-341
-
reversible inhibitor
S-(3'-chlorophenyl)-6-(chloromethyl)-2-oxo-2H-1-benzopyran-3-carbothioate
-
-
SDS
1%, activity is reduced 6fold
SERPINB3
partial inhibition
-
SERPINB4
partial inhibition
-
Soybean trypsin inhibitor
-
STI
-
soybean trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected
-
Suc-Val-Pro-PheP(OPh)2
-
most potent phosphonate chymotrypsin inhibitor
symplocamide A
-
from the Marine Cyanobacterium Symploca sp.. A potent cancer cell cytotoxin, which also inhibits serine proteases with a 200fold greater inhibition of chymotrypsin over trypsin
taraxast-20(39)-ene-3beta,16beta-diol
-
0.000096 mM, 50% inhibition, noncompetitive inhibition
taraxast-20-ene-3beta,16beta-diol
-
0.00016 mM, 50% inhibition, competitive inhibition
tert-butyl (2S)-2-([1-[bis(2-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(3,4-dimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-([1-[bis(4-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-[(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)carbamoyl]pyrrolidine-1-carboxylate
-
-
tert-butyl (2S)-2-[[1-(diphenoxyphosphoryl)-2-phenylethyl]carbamoyl]pyrrolidine-1-carboxylate
-
-
tert-butyl [(2S)-1-(3,4-dihydroquinolin-1(2H)-yl)-1-oxo-3-phenylpropan-2-yl]carbamate
isoquinoline amino acid conjugate, exhibits lesser inhibitory activity compared to the enediyne-peptide conjugates, does not interact directly with the catalytic triad of chymotrypsin
tert-butyl [(2S)-1-oxo-3-phenyl-1-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)propan-2-yl]carbamate
enediyne-amino acid conjugate
tirucalla-7,24-dien-3beta-ol
-
0.000098 mM, 50% inhibition, noncompetitive inhibition
TLCK
-
1 mM, 10% loss of activity
Toluene
-
10% DMSO decreases the activity to 85%. 30% DMSO reduces the activity to about 70%, and 50% DMSO reduces the activity to about 45%
tosyl-L-phenylalanine chloromethyl ketone
trypsin inhibitor I-S
-
from soybean
-
trypsin-inhibitor
-
from Glycine max cv. Small Glossy Black
-
turkey ovomucoid inhibitor
-
-
-
urs-12-ene-3beta,16beta-diol
-
0.00012 mM, 50% inhibition, noncompetitive inhibition
WCI2
-
Psophocarpus tetragonolobus chymotrypsin inhibitor retards growth of Helicoverpa armigera and strongly inhibits commercially available bovine chymotrypsin
-
WCI5
-
Psophocarpus tetragonolobus chymotrypsin inhibitor retards growth of Helicoverpa armigera and strongly inhibits commercially available bovine chymotrypsin
-
wheat subtilisin/chymotrypsin inhibitor
-
as the wild-type inhibitor, the recombinant inhibitor, while acting as a strong subtilisin inhibitor, is much less efficient against alpha-chymotrypsin. Mutant E49S of the wheat subtilisin/chymotrypsin inhibitor shows a limited anti-chymotrypsin activity. Mutants E49P, Y50G and M48P/E49G are inactive against alpha-chymotrypsin
-
winged bean chymotrypsin-trypsin inhibitor
-
inhibitor from Psophocarpus tetragonolobus, inhibits and binds trypsin and chymotrypsin in a 1:1 molar ratio. Complete inhibition of chymotrypsin with purified recombinant inhibitor at a 1:1 molar ratio, similarly to the inhibitor obtained from winged bean seeds
-
Aprotinin
-
-
Aprotinin
partial inhibition at 0.1 mg/ml
basic pancreatic trypsin inhibitor
-
engineering of inhibitors with altered specificities
-
basic pancreatic trypsin inhibitor
-
-
-
basic pancreatic trypsin inhibitor
-
moderate inhibition at pH 8.3, mutants of basic pancreatic trypsin inhibitor, i.e. BPTI, that strongly inhibit alpha-chymotrypsin are obtained by affinity selection of an M13 phage displayed library of BPTI variants
-
benzamidine
-
-
benzamidine
-
partially inhibits both chymotrypsin A and B. At room temperature, at 5 mM concentration, 60.3% and 62.5% residual activity for chymotrypsin A and B, respectively
benzamidine
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, inhibits chymotrypsin II, whereas chymotrypsin I is not inhibited
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
-
-
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
-
extremely potent inhibitor
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
-
-
chymostatin
-
-
chymostatin
competitive inhibition
chymostatin
-
effectively inhibits both chymotrypsin A and B. At room temperature, at 0.01 mM concentration, no residual activity for both chymotrypsin A and B
chymostatin
-
strong inhibitory effect in concentrations starting from 0.001 mM, 50% inhibition by 0.05 mM
chymostatin
-
0.02 mM, complete loss of activity
chymostatin
complete inhibition at 0.25 mM
chymostatin
-
0.01 mM, no residual activity
Cu2+
-
strongly inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 0% and 2.5% residual activity for chymotrypsin A and B, respectively
Cu2+
-
5 mM, 80% residual activity
Cu2+
-
5 mM, 1.4% residual activity
DFP
-
-
DFP
-
0.1 mM, 6.3% residual activity
EDTA
-
shows minimal inhibitory effect on both chymotrypsin A and B. At room temperature, at 1 mM concentration, 99% and 97% residual activity for chymotrypsin A and B, respectively
EDTA
-
5 mM, 580.7% residual activity
EDTA
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, inhibits chymotrypsin II, whereas chymotrypsin I is not inhibited
EDTA
10 mM, 50% loss of activity
Fe2+
-
strongly inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 14.6% and 21.1% residual activity for chymotrypsin A and B, respectively
Fe2+
-
5 mM, 73% residual activity
Fe2+
-
5 mM, 6% residual activity
Hg2+
-
5 mM, 44% residual activity
Hg2+
-
5 mM, 12% residual activity
lima bean inhibitor
-
-
-
lima bean inhibitor
-
-
-
lima bean inhibitor
-
-
-
Lima bean trypsin inhibitor
-
-
-
Lima bean trypsin inhibitor
-
-
-
N-alpha-tosyl-L-Phe chloromethyl ketone
-
TPCK
N-alpha-tosyl-L-Phe chloromethyl ketone
-
TPCK
N-alpha-tosyl-L-Phe chloromethyl ketone
TPCK
N-alpha-tosyl-L-Phe chloromethyl ketone
TPCK
N-alpha-tosyl-L-Phe chloromethyl ketone
-
TPCK
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone
-
Z-GGF-CK
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone
-
Z-GGF-CK
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone
Z-GGF-CK
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone
Z-GGF-CK
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone
-
Z-GGF-CK
phenylmethylsulfonyl fluoride
-
1 mM, 95% loss of activity
phenylmethylsulfonyl fluoride
complete inhibition
phenylmethylsulfonyl fluoride
-
5 mM, 0.7% residual activity
phenylmethylsulfonyl fluoride
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits chymotrypsin I and chymotrypsin II (1-2% residual activity)
phenylmethylsulfonyl fluoride
-
0.1 mM, 1.4% residual activity
Phenylmethylsulfonylfluoride
-
the enzymatic activity is completely lost at 1 mM and room temperature
Phenylmethylsulfonylfluoride
complete inhibition at 2 mM
Phenylmethylsulfonylfluoride
-
1% residual activity at 1.4 mg/ml
Phenylmethylsulfonylfluoride
-
Proflavin
-
Proflavin
-
substrate mimic inhibitor
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits (17% residual activity)
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
complete inhibition at 0.1 mg/ml
-
Soybean trypsin inhibitor
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, fully inhibits chymotrypsin I and chymotrypsin II (no residual activity)
-
Soybean trypsin inhibitor
-
6% residual activity at 0.5 mg/ml
-
Soybean trypsin inhibitor
-
0.0015 mM, 2.1% residual activity
-
tosyl-L-phenylalanine chloromethyl ketone
-
5 mM, 5.8% residual activity
tosyl-L-phenylalanine chloromethyl ketone
-
TPCK. Inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected
TPCK
-
-
TPCK
-
1 mM, 10% loss of activity
WSCI
-
proteinase inhibitor isolated from Triticum aestivum
WSCI
-
proteinase inhibitor isolated from Triticum aestivum
additional information
-
an inhibitor from Arachis hypogaea
-
additional information
not inhibited by 4',6-diamidino-2-phenylindole
-
additional information
-
protease inhibitor does not exert any inhibitory effect on hepatoma (Hep G2) and breast cancer (MCF 7) cells or antifungal action toward Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola
-
additional information
novel synthesized enediyne-amino acid conjugates effectively target chymotrypsin inhibiting its proteolytic activity. Mode of inhibition is mostly competitive or of a mixed type depending on the nature of the inhibitor
-
additional information
-
at 25°C, pH 8.0, in 50 mM Tris-HCl buffer and 10 mM CaCl2, is not inhibited by benzamidine and EDTA
-
additional information
-
substitution of the phosphonate diphenyl ester rings by a variety of different substituents at different positions does not significantly improve inhibitory activity
-
additional information
-
chymotrypsin inhibitory activity of protease inhibitor extract of chickpea resistant cultivar treated with salicylic acid and spermine, respectively, is increased by 92 UI/microg protein (52% over control), 72 UI/microg protein (38% over control) in roots and 79 UI/microg protein (63% over control), 66 UI/microg protein (57% over control) in shoots compared with their respective controls, whereas protease inhibitor extract of susceptible cultivar roots and shoots show 20-30% increase in chymotrypsin inhibitory activity over their respective controls
-
additional information
inhibitory activity of the urea compounds synthesized depends upon the position of the methyl group at the phenyl ring and the presence of substituents at the nitrogen of the urea bridge. The inhibitory trend suggests that alpha-chymotrypsin inhibitory activity declines with ortho, meta, para substitution order
-
additional information
-
no inhibition by N-p-tosyl-lysine chloroketone and 4-(amidinopheyl)methanesulfonyl fluoride
-
additional information
-
overview about structure-activity relationships for all Ahp-containing depsipeptides with inhibitory activity against trypsin or chymotrypsin
-
additional information
-
boron peptides selectively inhibit the chymotrypsin-like activity (beta5) of 20S proteasome
-
additional information
-
E-64 and pepstatin A have no effect on enzyme activity
-
additional information
not inhibited by benzamide and L-1-chloro-3-(4-tosylamido)-7-amino-2-heptanone
-
additional information
-
not inhibited by 0.25 mg/ml EDTA or 2 mg/ml benzamidine
-
additional information
-
affinity of Chtr1 to inhibitors containing Arg at P1 position. N-alpha-tosyl-L-lysine chloromethyl ketone (TLCK), Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition
-
additional information
-
efficient binding and inhibition of ChT by a self-assembled DNA quadruplex with protein recognition fragments appended on the 5'-ends of the assembled G-quartet: quadruplex containing two anionic residues in each loop (GDGD) displays the highest inhibition potency for ChT, exhibits slow binding inhibition. Quadruplex containing one anionic and one hydrophobic residue (GDGY) strongly inhibits ChT activity, exhibits slow binding inhibition. Quadruplex without tethered loops or containing a peptide loop tethered to a single strand of a sequence that is incapable of forming self-assembled structures, are weak inhibitors
-
additional information
-
lowest inhibition concentrations of chymotrypsin for glyceric acid at position 7, while 3-sulfoglyceric acid derivatives are less potent and disulfoglyceric acid derivatives exert much higher IC50's
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.5 - 1.4
4-nitrophenyl acetate
0.032 - 0.098
4-nitrophenyl benzoate
7.27
acetyl-L-Leu methyl ester
-
-
0.17
acetyl-L-Trp ethyl ester
-
-
1
acetyl-Leu methyl ester
-
-
1.04 - 1.39
acetyl-Phe ethyl ester
1.12
acetyl-Trp ethyl ester
-
-
1.27 - 2
Acetyl-Tyr ethyl ester
0.59 - 6.79
Ala-Ala-Phe-7-amido-4-methylcoumarin
0.44
benzoyl-4-guanidinophenyl ester
-
25°C, pH 7.6
4.94
benzoyl-D-Ala-4-guanidinophenyl ester
-
25°C, pH 7.6
1.49
benzoyl-D-Leu-4-guanidinophenyl ester
-
25°C, pH 7.6
2.58
benzoyl-D-Phe-4-guanidinophenyl ester
-
25°C, pH 7.6
2.81
benzoyl-Gly-4-guanidinophenyl ester
-
25°C, pH 7.6
2.88
benzoyl-L-Ala-4-guanidinophenyl ester
-
25°C, pH 7.6
0.13 - 0.64
benzoyl-Tyr ethyl ester
7.02 - 8.72
N-acetyl-L-leucine methyl ester
0.0891
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid
-
-
0.0101
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid
-
-
0.1131
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid
-
-
0.1203
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid
-
-
0.0635
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid
-
-
0.001
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
0.0191
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid
-
-
0.0598
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
1.12 - 2.58
N-acetyl-L-phenylalanine ethyl ester
0.14 - 0.19
N-acetyl-L-tryptophan ethyl ester
0.57 - 1.17
N-acetyl-L-tyrosine ethyl ester
0.939
N-alpha-benzoyl-DL-arginine-p-nitroanilide
-
-
0.1 - 0.27
N-benzoyl-L-Tyr ethyl ester
0.01 - 0.18
N-benzoyl-L-tyrosine 4-nitroanilide
0.12 - 8.1
N-benzoyl-L-tyrosine ethyl ester
2.23 - 20.6
N-benzoyl-L-tyrosine methyl ester
0.05 - 0.083
N-benzoyl-L-tyrosine-4-nitroanilide
0.067
N-benzoyl-L-tyrosyl-ethyl ester
pH 8.0, 37°C
0.08 - 0.21
N-benzoyl-Tyr p-nitroanilide
0.0009
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
0.0177
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
0.32 - 6.1
N-glutaryl-L-Phe-p-nitroanilide
0.44
N-glutaryl-L-phenylalanine-4-nitroanilide
pH 7.75, 25°C
0.0013 - 0.023
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
0.32 - 3
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
0.0346
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
0.26 - 0.44
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
0.32 - 2.6
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
0.02 - 0.37
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
0.052 - 0.58
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
0.16 - 1.6
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
0.05 - 0.19
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
0.012
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.0074 - 1.1
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.4 - 0.48
N-succinyl-Ala-Phe-Ala
2.5
N-succinyl-Ala-Phe-p-nitroanilide
-
30°C, pH 8.0
0.044
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide
-
at 25°C
0.074
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide
-
in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C
0.14
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 25°C
1.09
N-succinyl-L-Phe-p-nitroanilide
-
at 25°C
0.024 - 0.375
N-succinyl-L-phenylalanine-4-nitroanilide
0.012
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.01 - 0.032
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
2.3 - 5
N-succinyl-Phe-7-amido-4-methylcoumarin
0.09 - 0.1
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
0.62 - 0.64
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester
0.08
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester
-
-
0.4
Nalpha-tosyl-L-Tyr benzyl ester
-
-
0.0297 - 0.044
p-nitrophenyl acetate
0.072
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 9.0, 25°C
0.022 - 0.07
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
0.048 - 0.074
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.012
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
37°C, pH 8.0
0.28
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide
-
pH 8.0, 25°C
0.09
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide
-
at 30°C
0.054
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
pH 8.5, 25°C
0.022 - 0.12
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
0.0005 - 0.8
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
additional information
casein
0.5
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.7
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.7
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.7
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
0.82
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
1.4
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.032
4-nitrophenyl benzoate
presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.035
4-nitrophenyl benzoate
presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.048
4-nitrophenyl benzoate
presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.053
4-nitrophenyl benzoate
pH 7.8, 27°C
0.06
4-nitrophenyl benzoate
presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.08
4-nitrophenyl benzoate
presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
0.098
4-nitrophenyl benzoate
presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
1.04
acetyl-Phe ethyl ester
-
-
1.39
acetyl-Phe ethyl ester
-
-
1.27
Acetyl-Tyr ethyl ester
-
-
2
Acetyl-Tyr ethyl ester
-
-
0.59
Ala-Ala-Phe-7-amido-4-methylcoumarin
pH 7.8, temperature not specified in the publication, enzyme adsorbed silica particles
1.05
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
10°C
1.1
Ala-Ala-Phe-7-amido-4-methylcoumarin
pH 7.8, temperature not specified in the publication, soluble enzyme
3.91
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
20°C
4.37
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
50°C
4.73
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
30°C
6.79
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
40°C
0.13
benzoyl-Tyr ethyl ester
-
-
0.64
benzoyl-Tyr ethyl ester
-
-
7.02
N-acetyl-L-leucine methyl ester
-
25°C, pH 7.5
8.72
N-acetyl-L-leucine methyl ester
-
25°C, pH 7.5
1.12
N-acetyl-L-phenylalanine ethyl ester
-
25°C, pH 7.5
2.58
N-acetyl-L-phenylalanine ethyl ester
-
25°C, pH 7.5
0.14
N-acetyl-L-tryptophan ethyl ester
-
25°C, pH 7.5
0.19
N-acetyl-L-tryptophan ethyl ester
-
25°C, pH 7.5
0.57
N-acetyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
1.17
N-acetyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
0.1
N-benzoyl-L-Tyr ethyl ester
-
enzyme form B, at 35°C
0.12
N-benzoyl-L-Tyr ethyl ester
-
enzyme form A, at 10°C
0.14
N-benzoyl-L-Tyr ethyl ester
-
enzyme form A, at 25°C
0.17
N-benzoyl-L-Tyr ethyl ester
-
enzyme form A, at 35°C
0.2
N-benzoyl-L-Tyr ethyl ester
-
enzyme form B, at 25°C or 10°C
0.24
N-benzoyl-L-Tyr ethyl ester
-
at 35°C
0.27
N-benzoyl-L-Tyr ethyl ester
-
at 25°C or at 10°C
0.01
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 7% dimethyl sulfoxide, pH 8.7, 25°C
0.056
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 15% dimethyl sulfoxide, pH 8.7, 25°C
0.16
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 7% dimethyl sulfoxide-water, pH 8.7, 25°C
0.18
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 20% dimethyl sulfoxide-water, pH 8.7, 25°C
0.12
N-benzoyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
0.13
N-benzoyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
1.17
N-benzoyl-L-tyrosine ethyl ester
pH 8.0, 30°C, soluble enzyme
1.35 - 8.1
N-benzoyl-L-tyrosine ethyl ester
pH 8.0, 30°C, glyoxal-agarose immobilized enzymes
3.33
N-benzoyl-L-tyrosine ethyl ester
-
transesterfication in 30% methanol
6.02
N-benzoyl-L-tyrosine ethyl ester
-
transesterfication in 10% methanol
2.23
N-benzoyl-L-tyrosine methyl ester
-
transesterfication in 10% ethanol
20.6
N-benzoyl-L-tyrosine methyl ester
-
transesterfication in 90% ethanol
0.05
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C
0.07
N-benzoyl-L-tyrosine-4-nitroanilide
pH 7.6, 25°C
0.083
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C
0.08
N-benzoyl-Tyr p-nitroanilide
-
enzyme form A and enzyme form B, at 25°C
0.09
N-benzoyl-Tyr p-nitroanilide
-
enzyme form A, at 10°C
0.11
N-benzoyl-Tyr p-nitroanilide
-
enzyme form B, at 35°C
0.11
N-benzoyl-Tyr p-nitroanilide
-
at 10°C
0.11
N-benzoyl-Tyr p-nitroanilide
-
enzyme form B, at 10°C
0.14
N-benzoyl-Tyr p-nitroanilide
-
at 25°C
0.14
N-benzoyl-Tyr p-nitroanilide
-
enzyme form A, at 35°C
0.21
N-benzoyl-Tyr p-nitroanilide
-
at 35°C
0.32
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.75
0.36
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.75, in the presence of 10 mM tetrabutylammonium bromide
0.94
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.75, in the presence of 2.5 mM NaCl
1
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.75, in the presence of 2 mM NaClO4
3.2
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.75, in the presence of 1000 mM tetrabutylammonium bromide
6.1
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.75, in the presence of 400 mM tetrabutylammonium bromide
0.0013
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
-
0.006
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
0.01
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
-
0.013
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
-
0.023
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
0.32
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
3
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.26
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.44
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.32
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M KCl
0.49
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 800 mM NaF
0.51
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.61
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaBr
0.92
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M LiCl
2.6
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.02
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.37
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.052
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.58
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.16
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
1.6
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.05
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
alpha-chymotrypsin, unmodified
0.07
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 2.2
0.07
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG700-alpha-chymotrypsin, degree of PEGylation: 3.8
0.08
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 1.3
0.08
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 2.9
0.08
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 4.2
0.08
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 6.0
0.09
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 4.2
0.1
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 1.3
0.11
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 7.6
0.112
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
0.15
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 8.4
0.15
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG700-alpha-chymotrypsin, degree of PEGylation: 7.9
0.19
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 9.1
0.0074
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.0087
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.02
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
chymotrypsin 4
0.2
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
0.3
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
-
0.5
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
-
0.5
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
-
1.1
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
0.4
N-succinyl-Ala-Phe-Ala
-
30°C, pH 8.0, determined by competition with N-succinyl-Ala-Phe-p-nitroanilide
0.48
N-succinyl-Ala-Phe-Ala
-
30°C, pH 8.0, HPLC-based assay
0.024
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
0.044
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
0.062
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
0.065
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
0.109
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
0.254
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
0.303
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
0.359
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication
0.375
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
0.01
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
0.022
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
-
0.022
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
-
0.025
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
0.032
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
-
2.3
N-succinyl-Phe-7-amido-4-methylcoumarin
-
-
2.4
N-succinyl-Phe-7-amido-4-methylcoumarin
-
3
N-succinyl-Phe-7-amido-4-methylcoumarin
-
-
3
N-succinyl-Phe-7-amido-4-methylcoumarin
-
-
5
N-succinyl-Phe-7-amido-4-methylcoumarin
-
0.09
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
-
at pH 6.0
0.1
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
-
at pH 4.0
0.62
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester
-
at pH 6.0
0.64
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester
-
at pH 4.0
0.0297
p-nitrophenyl acetate
-
in the presence of 20 mM hexadecyltrimethylammonium bromide, at 25°C
0.044
p-nitrophenyl acetate
-
at 25°C
0.022
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
37°C, pH 8.0
0.07
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.048
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 30°C, chymotrypsin II
0.074
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 30°C, chymotrypsin I
0.022
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
-
pH 8.5, 25°C
0.12
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
pH 8.0, 25°C
0.0005
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin B, at pH 7.5 and 37°C
0.0014
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin A, at pH 7.5 and 37°C
0.8
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
at pH 7.5 and 37°C
additional information
casein
hydrolysis kinetics are analysed in the framework of a two-step model, with consecutive demasking and hydrolysis steps. Determination of kinetic parameters for casein hydrolysis by chymotrypsin using two ranges of substrate concentration
additional information
additional information
-
Michaelis-Menten metabolic rate constants (Vmax and Km) are calculated by nonlinear regression for chymotrypsin, the Vmax is 97.97 nmol/l/min and Km is 7.84 microM
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.015 - 0.1
4-nitrophenyl acetate
0.0075 - 0.047
4-nitrophenyl benzoate
6.6
acetyl-L-Leu methyl ester
-
-
48.4
acetyl-L-Trp ethyl ester
-
-
22.1
acetyl-Leu methyl ester
-
-
5.3 - 52.4
acetyl-Phe ethyl ester
9.3
acetyl-Trp ethyl ester
-
-
25.4 - 126
Acetyl-Tyr ethyl ester
0.45 - 21.7
Ala-Ala-Phe-7-amido-4-methylcoumarin
0.13
benzoyl-4-guanidinophenyl ester
-
25°C, pH 7.6
0.56 - 6.08
benzoyl-D-Ala-4-guanidinophenyl ester
3 - 6
benzoyl-D-Leu-4-guanidinophenyl ester
5.54
benzoyl-D-Phe-4-guanidinophenyl ester
-
25°C, pH 7.6
1.18 - 6.08
benzoyl-Gly-4-guanidinophenyl ester
10.4
benzoyl-L-Ala-4-guanidinophenyl ester
-
25°C, pH 7.6
57.5 - 76.6
benzoyl-Tyr ethyl ester
4.6 - 6.5
N-acetyl-L-leucine methyl ester
6.4
N-acetyl-L-Phe-Ala-Thr-Pal-5-amido-2-nitrobenzoic acid
-
-
3
N-acetyl-L-Phe-Ala-Thr-Phe-(p-CN)-5-amido-2-nitrobenzoic acid
-
-
0.3
N-acetyl-L-Phe-Ala-Thr-Phe-(p-COOMe)-5-amido-2-nitrobenzoic acid
-
-
6.5
N-acetyl-L-Phe-Ala-Thr-Phe-(p-guanidine)-5-amido-2-nitrobenzoic acid
-
-
16.2
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NH2)-5-amido-2-nitrobenzoic acid
-
-
0.6
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
6.1
N-acetyl-L-Phe-Ala-Thr-Phe-5-amido-2-nitrobenzoic acid
-
-
22.7
N-acetyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
40.3 - 54.8
N-acetyl-L-phenylalanine ethyl ester
25.7 - 48.8
N-acetyl-L-tryptophan ethyl ester
130 - 220
N-acetyl-L-Tyr-ethyl ester
0.1 - 0.19
N-acetyl-L-Tyr-p-nitroanilide
82.8 - 120
N-acetyl-L-tyrosine ethyl ester
0.611
N-alpha-benzoyl-DL-arginine-p-nitroanilide
-
-
43 - 329
N-benzoyl-L-Tyr ethyl ester
0.013 - 2.3
N-benzoyl-L-tyrosine 4-nitroanilide
0.03 - 70.4
N-benzoyl-L-tyrosine ethyl ester
2.34 - 10.8
N-benzoyl-L-tyrosine methyl ester
0.04 - 0.5
N-benzoyl-L-tyrosine-4-nitroanilide
0.00942
N-benzoyl-L-tyrosyl-ethyl ester
pH 8.0, 37°C
0.05 - 0.82
N-benzoyl-Tyr p-nitroanilide
1.8
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Phe-(p-NO2)-5-amido-2-nitrobenzoic acid
-
-
16.7
N-benzyloxycarbonyl-L-Phe-Ala-Thr-Tyr-5-amido-2-nitrobenzoic acid
-
-
0.015 - 0.31
N-glutaryl-L-Phe-p-nitroanilide
0.0146
N-glutaryl-L-phenylalanine-4-nitroanilide
pH 7.75, 25°C
0.014 - 0.05
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
0.55
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
0.031 - 16
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
0.006 - 0.34
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
1.45 - 15
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
12 - 18
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
0.04 - 9.31
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
3.8 - 13.5
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
42
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
10.58 - 110
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
0.9 - 1.2
N-succinyl-Ala-Phe-Ala
0.65
N-succinyl-Ala-Phe-p-nitroanilide
-
30°C, pH 8.0
39
N-succinyl-L-Ala-Ala-Phe-p-nitroanilide
-
at 25°C
18.6
N-succinyl-L-Ala-Ala-Pro-p-nitroanilide
-
in 50 mM Tris-HCl pH 8.0 with 10 mM CaCl2, at 30 °C
7.8
N-succinyl-L-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 25°C
0.014
N-succinyl-L-Phe-p-nitroanilide
-
at 25°C
0.154 - 1.177
N-succinyl-L-phenylalanine-4-nitroanilide
23.9
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
0.008 - 6.43
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
0.6
Nalpha-benzyloxycarbonyl-L-Lys p-nitrophenyl ester
-
at pH 4.0
85
Nalpha-benzyloxycarbonyl-L-Tyr p-nitrophenyl ester
-
-
21.4
Nalpha-tosyl-L-Tyr benzyl ester
-
-
0.017 - 0.028
p-nitrophenyl acetate
19.24
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 9.0, 25°C
98.3
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
37°C, pH 8.0
4.8 - 18.6
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
105
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
37°C, pH 8.0
1.07
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide
-
pH 8.0, 25°C
1.9
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide
-
at 30°C
3.6
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
pH 8.5, 25°C
18 - 21.5
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
0.9 - 3.4
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
additional information
benzoyl-D-Phe-4-guanidinophenyl ester
0.015
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.024
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.046
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.046
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
0.062
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
0.1
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.0075
4-nitrophenyl benzoate
pH 7.8, 27°C
0.0086
4-nitrophenyl benzoate
presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.013
4-nitrophenyl benzoate
presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.017
4-nitrophenyl benzoate
presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.0214
4-nitrophenyl benzoate
presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.024
4-nitrophenyl benzoate
presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
0.047
4-nitrophenyl benzoate
presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
5.3
acetyl-Phe ethyl ester
-
-
52.4
acetyl-Phe ethyl ester
-
-
25.4
Acetyl-Tyr ethyl ester
-
-
126
Acetyl-Tyr ethyl ester
-
-
0.45
Ala-Ala-Phe-7-amido-4-methylcoumarin
pH 7.8, temperature not specified in the publication, enzyme adsorbed silica particles
0.6
Ala-Ala-Phe-7-amido-4-methylcoumarin
pH 7.8, temperature not specified in the publication, soluble enzyme
0.66
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
10°C
4.13
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
20°C
8.05
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
50°C
12.8
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
30°C
21.7
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
40°C
0.56
benzoyl-D-Ala-4-guanidinophenyl ester
-
25°C, pH 7.6
6.08
benzoyl-D-Ala-4-guanidinophenyl ester
-
25°C, pH 7.6
3 - 6
benzoyl-D-Leu-4-guanidinophenyl ester
-
25°C, pH 7.6
3.04
benzoyl-D-Leu-4-guanidinophenyl ester
-
25°C, pH 7.6
1.18
benzoyl-Gly-4-guanidinophenyl ester
-
25°C, pH 7.6
6.08
benzoyl-Gly-4-guanidinophenyl ester
-
25°C, pH 7.6
57.5
benzoyl-Tyr ethyl ester
-
-
76.6
benzoyl-Tyr ethyl ester
-
-
4.6
N-acetyl-L-leucine methyl ester
-
25°C, pH 7.5
6.5
N-acetyl-L-leucine methyl ester
-
25°C, pH 7.5
40.3
N-acetyl-L-phenylalanine ethyl ester
-
25°C, pH 7.5
54.8
N-acetyl-L-phenylalanine ethyl ester
-
25°C, pH 7.5
25.7
N-acetyl-L-tryptophan ethyl ester
-
25°C, pH 7.5
48.8
N-acetyl-L-tryptophan ethyl ester
-
25°C, pH 7.5
130
N-acetyl-L-Tyr-ethyl ester
-
25°C, pH 7.8, in non-denaturating water-acetone oxime mixture
192
N-acetyl-L-Tyr-ethyl ester
-
25°C, pH 7.8, in non-denaturating water-1,4-butanediol mixture
205
N-acetyl-L-Tyr-ethyl ester
-
20°C, pH 8.0, in non-denaturating water-dimethylsulfoxide mixture
220
N-acetyl-L-Tyr-ethyl ester
-
20°C, pH 8.0, in non-denaturating water-ethanol mixture
0.1
N-acetyl-L-Tyr-p-nitroanilide
-
20°C, pH 8.0, in non-denaturating water-dimethylsulfoxide mixture
0.19
N-acetyl-L-Tyr-p-nitroanilide
-
20°C, pH 8.0, in non-denaturating water-ethanol mixture
82.8
N-acetyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
119.5
N-acetyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
120
N-acetyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
43
N-benzoyl-L-Tyr ethyl ester
-
at 10°C
100
N-benzoyl-L-Tyr ethyl ester
-
at 35°C
117
N-benzoyl-L-Tyr ethyl ester
-
enzyme form A, at 10°C
120
N-benzoyl-L-Tyr ethyl ester
-
enzyme form B, at 10°C
142
N-benzoyl-L-Tyr ethyl ester
-
at 35°C
207
N-benzoyl-L-Tyr ethyl ester
-
enzyme form A, at 25°C
214
N-benzoyl-L-Tyr ethyl ester
-
enzyme form B, at 32°C
276
N-benzoyl-L-Tyr ethyl ester
-
enzyme form B, at 35°C
329
N-benzoyl-L-Tyr ethyl ester
-
enzyme form A, at 35°C
0.013
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 15% dimethyl sulfoxide, pH 8.7, 25°C
0.04
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 7% dimethyl sulfoxide-water, pH 8.7, 25°C
0.055
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 20% dimethyl sulfoxide-water, pH 8.7, 25°C
2.3
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 7% dimethyl sulfoxide, pH 8.7, 25°C
0.03 - 0.55
N-benzoyl-L-tyrosine ethyl ester
-
transesterfication in 10% methanol
0.031 - 0.51
N-benzoyl-L-tyrosine ethyl ester
-
transesterfication in 30% methanol
1.3
N-benzoyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
6.77
N-benzoyl-L-tyrosine ethyl ester
-
transesterfication in 10% methanol
8.13
N-benzoyl-L-tyrosine ethyl ester
-
transesterfication in 30% methanol
45.9
N-benzoyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
70.4
N-benzoyl-L-tyrosine ethyl ester
-
25°C, pH 7.5
2.34
N-benzoyl-L-tyrosine methyl ester
-
transesterfication in 10% ethanol
2.94
N-benzoyl-L-tyrosine methyl ester
-
transesterfication in 10% ethanol
10.8
N-benzoyl-L-tyrosine methyl ester
-
transesterfication in 90% ethanol
0.04
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C
0.15
N-benzoyl-L-tyrosine-4-nitroanilide
pH 7.6, 25°C
0.5
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C
0.05
N-benzoyl-Tyr p-nitroanilide
-
at pH 10°C
0.1
N-benzoyl-Tyr p-nitroanilide
-
enzyme form A and enzyme form B, at pH 10°C
0.3
N-benzoyl-Tyr p-nitroanilide
-
at 25°C
0.41
N-benzoyl-Tyr p-nitroanilide
-
at 35°C
0.41
N-benzoyl-Tyr p-nitroanilide
-
enzyme form A, 25°C
0.42
N-benzoyl-Tyr p-nitroanilide
-
enzyme form B, at 25°C
0.72
N-benzoyl-Tyr p-nitroanilide
-
enzyme form B, at 25°C
0.82
N-benzoyl-Tyr p-nitroanilide
-
enzyme form A, at 35°C
0.015
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.8
0.017
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.8, in the presence of 10 mM tetrabutylammonium bromide
0.025
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.8, in the presence of 1000 mM tetrabutylammonium bromide
0.034
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.8, in the presence of 2 mM NaClO4
0.039
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.8, in the presence of 2.5 mM NaCl
0.31
N-glutaryl-L-Phe-p-nitroanilide
-
25°C, pH 7.8, in the presence of 400 mM tetrabutylammonium bromide
0.014
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.05
N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.031 - 0.51
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
8.04
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
16
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.006
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.01
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M LiCl
0.026
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M KCl
0.26
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.27
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 800 mM NaF
0.34
N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaBr
1.45
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
15
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
12
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
18
N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
0.04 - 1.97
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
3.3
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
5.32
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
9.31
N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
3.8
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
6.2
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG700-alpha-chymotrypsin, degree of PEGylation: 7.9
7.6
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 4.2
7.9
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 9.1
8.3
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 6.0
8.6
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 7.6
8.7
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 4.2
9.4
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 8.4
10.2
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 2.9
10.7
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 2.2
11.6
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG700-alpha-chymotrypsin, degree of PEGylation: 3.8
12.1
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
alpha-chymotrypsin, unmodified
13.1
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG5000-alpha-chymotrypsin, degree of PEGylation: 1.3
13.5
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
PEG2000-alpha-chymotrypsin, degree of PEGylation: 1.3
10.58
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
chymotrypsin 4
77
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2, 3 M NaCl
110
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
21°C, pH 8.3, 20 mM CaCl2
0.9
N-succinyl-Ala-Phe-Ala
-
30°C, pH 8.0, determined by competition with N-succinyl-Ala-Phe-p-nitroanilide
1.2
N-succinyl-Ala-Phe-Ala
-
30°C, pH 8.0, HPLC-based assay
0.154
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
0.236
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
0.362
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
0.417
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
0.48
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
0.807
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
0.985
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication
1.054
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
1.177
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
0.008
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
-
at pH 4.0
0.3
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
-
at pH 6.0
6.43
Nalpha-benzyloxycarbonyl-L-Ala p-nitrophenyl ester
-
at pH 6.0
0.017
p-nitrophenyl acetate
-
in 20 mM Tris/HCl pH 7.5 at 25°C
0.028
p-nitrophenyl acetate
-
in the presence of 20 mM hexadecyltrimethylammonium bromide, in 20 mM Tris/HCl pH 7.5 at 25°C
4.8
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 30°C, chymotrypsin II
18.6
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
at 30°C, chymotrypsin I
18
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
-
pH 8.5, 25°C
21.5
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
pH 8.0, 25°C
0.9
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
at pH 7.5 and 37°C
2.7
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin A, at pH 7.5 and 37°C
3.4
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin B, at pH 7.5 and 37°C
additional information
benzoyl-D-Phe-4-guanidinophenyl ester
-
25°C, pH 7.6
additional information
additional information
-
-
-
additional information
additional information
-
influence of dimethylsulfoxide on the turnover-number
-
additional information
additional information
adsorption of the enzyme to silica particles leads to an 8fold increase of the turnover number
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.021 - 0.144
4-nitrophenyl acetate
0.141 - 0.668
4-nitrophenyl benzoate
0.23 - 220
N-benzoyl-L-tyrosine 4-nitroanilide
0.48 - 10
N-benzoyl-L-tyrosine-4-nitroanilide
0.14
N-benzoyl-L-tyrosyl-ethyl ester
pH 8.0, 37°C
0.0332
N-glutaryl-L-phenylalanine-4-nitroanilide
pH 7.75, 25°C
16
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
34
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 8.5, temperature not specified in the publication
3500
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
2.74 - 6.47
N-succinyl-L-phenylalanine-4-nitroanilide
2000
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
pH 8.5, temperature not specified in the publication
2670
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 9.0, 25°C
3.8
succinyl-L-Ala-Ala-Pro-L-Phe-4-nitroanilide
-
pH 8.0, 25°C
21
succinyl-L-Ala-Ala-Pro-L-phenylalanine-p-nitroanilide
-
at 30°C
70
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
pH 8.5, 25°C
179 - 820
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
1100 - 6800
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
0.021
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.033
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.048
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 20, pH 7.8, 27°C
0.056
4-nitrophenyl acetate
25 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
0.088
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5, pH 7.8, 27°C
0.144
4-nitrophenyl acetate
5 mM sodium 1,4-bis(2-ethylhexyl)sulfosuccinate, ratio [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, pH 7.8, 27°C
0.141
4-nitrophenyl benzoate
pH 7.8, 27°C
0.143
4-nitrophenyl benzoate
presence of 0.5 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.247
4-nitrophenyl benzoate
presence of 0.5 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
0.345
4-nitrophenyl benzoate
presence of 0.5 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.365
4-nitrophenyl benzoate
presence of 2.0 mM cetyltrimethylammonium bromide, pH 7.8, 27°C
0.583
4-nitrophenyl benzoate
presence of 2.0 mM cetyltriphenylphosphonium bromide, pH 7.8, 27°C
0.668
4-nitrophenyl benzoate
presence of 2.0 mM cetyltributylphosphonium bromide, pH 7.8, 27°C
0.23
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 15% dimethyl sulfoxide, pH 8.7, 25°C
0.254
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 7% dimethyl sulfoxide-water, pH 8.7, 25°C
0.305
N-benzoyl-L-tyrosine 4-nitroanilide
water-dimethyl sulfoxide mixture encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles, 20% dimethyl sulfoxide-water, pH 8.7, 25°C
220
N-benzoyl-L-tyrosine 4-nitroanilide
presence of 7% dimethyl sulfoxide, pH 8.7, 25°C
0.48
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)6, pH 7.6, 25°C
2.14
N-benzoyl-L-tyrosine-4-nitroanilide
pH 7.6, 25°C
10
N-benzoyl-L-tyrosine-4-nitroanilide
presence of alpha,omega-alkanedyl-bis(hydroxyethylmethylcetyl) dibromide with a polymethylene spacer n-(CH2)10, pH 7.6, 25°C
2.74
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, pH 7.7, temperature not specified in the publication
3.14
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
3.18
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
3.48
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
4.44
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.1 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
5.35
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-1-methylpyrrolidinium, pH 7.7, temperature not specified in the publication
5.87
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-hexyl-3-methylimidazolium chloride, pH 7.7, temperature not specified in the publication
6.42
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-3-methylimidazoliumchloride, pH 7.7, temperature not specified in the publication
6.47
N-succinyl-L-phenylalanine-4-nitroanilide
presence of 2 mM cetyltrimethylammonium bromide, 0.5 M 1-butyl-4-methylpyridinium chloride, pH 7.7, temperature not specified in the publication
179
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
pH 8.0, 25°C
820
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
-
pH 8.5, 25°C
1100
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
at pH 7.5 and 37°C
1900
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin A, at pH 7.5 and 37°C
6800
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin
-
chymotrypsin B, at pH 7.5 and 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.08
(Cbz-alanyl)aminomethyl boronic acid
-
37°C, pH 8.0
1.96
(Cbz-phenylalanyl)aminomethyl boronic acid
-
37°C, pH 8.0
0.00001
2,2'-[[(1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphoryl]bis(oxybenzene-4,1-diyl)]diacetic acid
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0027
2,2'-[[(1-[[1-(tert-butoxycarbonyl)-L-prolyl]amino]-3-methylbutyl)phosphoryl]bis(oxybenzene-3,1-diyl)]diacetic acid
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0301
2-(2,6-difluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0277
2-(2,6-dimethoxyphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0091
2-(2-bromophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.00047
2-(2-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0125
2-(2-methoxyphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0098
2-(2-methylphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0224
2-(2-nitrophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0091
2-(2-thienyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0226
2-(3-chlorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0178
2-(3-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0161
2-(3-methylphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.01619
2-(3-methylphenyl)-7-nitro-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.341
2-(3-nitrophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.094
2-(4-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0126
2-(4-methoxyphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.0164
2-(4-methylphenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.03
2-oxo-2-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.75
2-oxo-2-(piperidin-1-yl)ethyl N-(tert-butoxycarbonyl)-L-phenylalaninate
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.0087
2-phenyl-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
31.01
4-fluorophenylboronic acid
-
37°C, pH 8.0
0.3363
5'-demethoxyepiexcelsin
-
-
0.303
7-chloro-2-(2-fluorophenyl)-4H-3,1-benzoxazin-4-one
pH 7.6, 30°C
0.00057
Ac-Phe-Val-Thr-p-amino-L-phenylalanine-CHO
-
-
0.0000032
Ac-Phe-Val-Thr-p-nitro-L-phenylalanine-CHO
-
-
0.000011
Ac-Phe-Val-Thr-Phe-CHO
-
-
0.001
Ac-Phe-Val-Thr-pyridyl-L-alanine-CHO
-
-
0.0000017
Ac-Phe-Val-Thr-Tyr-CHO
-
-
0.00001
Acacia plumosa trypsin inhibitor A
-
in phosphate buffer saline, pH 7.4, at 37°C for 30 min
-
0.0000102
Acacia plumosa trypsin inhibitor B
-
in phosphate buffer saline, pH 7.4, at 37°C for 30 min
-
0.0000109
Acacia plumosa trypsin inhibitor C
-
in phosphate buffer saline, pH 7.4, at 37°C for 30 min
-
0.00000000035
anti-trypsin-chymotrypsin A
-
-
-
0.0000000004
anti-trypsin-chymotrypsin B
-
-
-
0.0000075 - 0.000018
Aprotinin
0.344
benzyloxycarbonyl-Ala-Ala-Phe-glyoxal
-
at 25°C
0.000025 - 0.024
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
0.065
benzyloxycarbonyl-Phe-glyoxal
-
-
0.00085
benzyloxycarbonyl-Pro-Phe-glyoxal
-
37°, pH 7.4
0.00002
bis(2,3-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.02
bis(2-methylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.015
bis(3,4,5-trimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0062
bis(3,4-dimethylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00014
bis(3-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0015
bis(4-chlorophenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.000045
bis(4-methoxyphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0013
bis(4-tert-butylphenyl) (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0033
bis[4-(propan-2-yl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00065
bis[4-(sulfanylmethyl)phenyl] (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.11
brein 3-O-myristate
-
-
0.11
brein 3-O-palmitate
-
-
0.0026
chymostatin
pH 7.6, 30°C
0.014
complex of vanadate and benzohydroxamic acid
in 20 mM sodium cacodylate (pH 7.4) and 75% saturated ammonium sulfate
0.42
cycloart-24-ene-3beta-ol
-
-
0.06
dammara-20,24-dien-3beta-ol
-
-
0.0047
diphenyl (1-[[(benzyloxy)carbonyl]amino]-2-phenylethyl)phosphonate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.03
faradiol 3-O-myristate
-
-
0.058
faradiol 3-O-palmitate
-
-
0.000000027
IVNGEEAVPGSWPW
-
-
0.00013 - 0.00017
Lima bean trypsin inhibitor
-
0.057
lup-20(29)-ene-3beta,16beta-diol
-
-
0.026
maniladiol 3-O-myristate
-
-
0.12
maniladiol 3-O-palmitate
-
-
0.0052
micropeptin 478-A
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.072
micropeptin 478-B
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.1
micropeptin HU1021
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.1
micropeptin HU1041
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.1
micropeptin HU1069
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0196
micropeptin HU895A
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0054
micropeptin HU895B
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0028
micropeptin HU909
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.024
micropeptin HU975
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.0182
micropeptin HU989
-
at 37°C, in 50 mM Tris-HCl buffer, 100 mM NaCl, 1 mM CaCl2 and 1 mM HCl
0.00001
mixed monolayer protected gold cluster
-
-
-
0.012
N-(tert-butoxycarbonyl)-L-alanyl-N-[4-[2-(3-oxoprop-1-yn-1-yl)phenyl]but-3-yn-1-yl]-L-phenylalaninamide
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.00005
N-(tert-butoxycarbonyl)-L-valyl-N-(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00036
N-(tert-butoxycarbonyl)-L-valyl-N-[1-(diphenoxyphosphoryl)-2-phenylethyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00033
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00179
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.00075
N-(tert-butoxycarbonyl)-L-valyl-N-[1-[bis(4-tert-butylphenoxy)phosphoryl]-3-methylbutyl]-L-prolinamide
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.003432
N-acetyl-L-Phe-Ala-Thr-Pal aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.000323
N-acetyl-L-Phe-Ala-Thr-Phe aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.000159
N-acetyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.000281
N-acetyl-L-Phe-Ala-Thr-Tyr aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
0.0000112
N-tosyl-L-Phe-Ala-Thr-Phe-(p-NO2) aldehyde
-
in Tris-HCl buffer with 20 mM CaCl2, at pH 8.3
9.7
phenylboronic acid
-
37°C, pH 8.0
0.00067 - 0.0019
Soybean trypsin inhibitor
-
0.000074
Suc-Val-Pro-PheP(OPh)2
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.063
taraxast-20(39)-ene-3beta,16beta-diol
-
-
0.068
taraxast-20-ene-3beta,16beta-diol
-
-
0.0005
tert-butyl (2S)-2-([1-[bis(2-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0046
tert-butyl (2S)-2-([1-[bis(3,4,5-trimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0108
tert-butyl (2S)-2-([1-[bis(3,4-dimethylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.02
tert-butyl (2S)-2-([1-[bis(4-methoxyphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.02
tert-butyl (2S)-2-([1-[bis(4-methylphenoxy)phosphoryl]-3-methylbutyl]carbamoyl)pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.073
tert-butyl (2S)-2-[(1-[bis[4-(sulfanylmethyl)phenoxy]phosphoryl]-2-phenylethyl)carbamoyl]pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.0104
tert-butyl (2S)-2-[[1-(diphenoxyphosphoryl)-2-phenylethyl]carbamoyl]pyrrolidine-1-carboxylate
-
in 100 mM HEPES buffer, 500 mM NaCl, pH 7.5, 9% dimethyl sulfoxide
0.003
tert-butyl [(2S)-1-oxo-3-phenyl-1-(1,2,7,8-tetradehydro-5,6-dihydro-4-benzazecin-4(3H)-yl)propan-2-yl]carbamate
in 80 mM Tris HCl buffer, pH 7.8 and 3 M CaCl2
0.072
tirucalla-7,24-dien-3beta-ol
-
-
0.11
urs-12-ene-3beta,16beta-diol
-
-
0.00000188
winged bean chymotrypsin-trypsin inhibitor
-
Dixon plot analysis, same for recombinant inhibitor and inhibitor obtained from winged bean seeds
-
0.0000072 - 0.0000094
WSCI
0.0000075
Aprotinin
-
25°C, pH 7.5
0.000018
Aprotinin
-
25°C, pH 7.5
0.000025
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
-
25°, pH 7.0
0.00057
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
-
25°, pH 3.2
0.019
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
-
at pH 7.23 and 25°C
0.024
benzyloxycarbonyl-Ala-Pro-Phe-glyoxal
-
at pH 7.17 and 25°C
0.00013
Lima bean trypsin inhibitor
-
25°C, pH 7.5
-
0.00017
Lima bean trypsin inhibitor
-
25°C, pH 7.5
-
0.00067
Soybean trypsin inhibitor
-
25°C, pH 7.5
-
0.0019
Soybean trypsin inhibitor
-
25°C, pH 7.5
-
0.0000072
WSCI
-
-
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