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modified soluble cyanobacterial granule polypeptide + H2O
beta-aspartate-arginine + beta-aspartate-homoarginine + ?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
modified soluble cyanobacterial granule polypeptide + H2O
beta-aspartate-arginine + beta-aspartate-homoarginine + ?
in vitro guanidination of lysine side chains of soluble cyanobacterial granule polypeptide with o-methylisourea yields the nonproteinogenic amino acid homoarginine. The modified soluble cyanobacterial granule polypeptide consists of 51 mol% aspartate, 14 mol% arginine, and 35 mol% homoarginine. The modified cyanobacterial granule polypeptide can be digested in vitro using cyanophycinase, yielding a new dipeptide consisting of aspartate and homoarginine
-
-
?
modified soluble cyanobacterial granule polypeptide + H2O
beta-aspartate-arginine + beta-aspartate-homoarginine + ?
in vitro guanidination of lysine side chains of soluble cyanobacterial granule polypeptide with o-methylisourea yields the nonproteinogenic amino acid homoarginine. The modified soluble cyanobacterial granule polypeptide consists of 51 mol% aspartate, 14 mol% arginine, and 35 mol% homoarginine. The modified cyanobacterial granule polypeptide can be digested in vitro using cyanophycinase, yielding a new dipeptide consisting of aspartate and homoarginine
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
Thermosynechococcus vestitus
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
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N100A
site-directed mutagenesis, 40% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
N158A
site-directed mutagenesis, 41% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
N172A
site-directed mutagenesis, 0.02% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
N17A
site-directed mutagenesis, 60% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
N202A
site-directed mutagenesis, 8% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
Q101A
site-directed mutagenesis, 0.02% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
Q173A
site-directed mutagenesis, 0.5% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
R178A
site-directed mutagenesis, 1% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
R180A
site-directed mutagenesis, 0.01% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
R183a
site-directed mutagenesis, 0.01% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
S132A
site-directed mutagenesis, 0.002% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C
additional information
coexpression of cyanophycin and CGPase in the same plant without substrate degradation in planta by transient expression of CGPase CphB either in the plastid or cytosol, or the Pseudomonas alcaligenes CGPase CphE in the cytosol of CGP-producing Nicotiana tabacum plants. No cyanophycin degradation appears prior to cell homogenization independent of the CGPase produced. In crude plant extracts, only cytosolic CphE leads to a fast degradation of cyanophycin. CphE also shows higher stability and in vitro activity compared to both CphB variants
additional information
Gly101, Asp172, Gly173, Arg178, Arg180 and Arg183 form a conserved pocket adjacent to the catalytic Ser132, these are functionally critical residues. The cyanophycinase active site contains three absolutely conserved Arg residues (Arg178, Arg180 and Arg183), which line up along one side of the pocket
additional information
Thermosynechococcus vestitus
coexpression of cyanophycin and CGPase in the same plant without substrate degradation in planta by transient expression of CGPase CphB either in the plastid or cytosol, or the Pseudomonas alcaligenes CGPase CphE in the cytosol of CGP-producing Nicotiana tabacum plants. No cyanophycin degradation appears prior to cell homogenization independent of the CGPase produced. In crude plant extracts, only cytosolic CphE leads to a fast degradation of cyanophycin. CphE also shows higher stability and in vitro activity compared to both CphB variants
additional information
Thermosynechococcus vestitus
optimized transient expression in Nicotiana benthamiana plants to produce high amounts of active CGPase. Protein stability is increased by the translational fusion of CGPase to the green fluorescent protein or to the transit peptide of the small subunit of RuBisCO for peptide production in the chloroplasts
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Richter, R.; Hejazi, M.; Kraft, R.; Ziegler, K.; Lockau, W.
Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme
Eur. J. Biochem.
263
163-169
1999
Synechocystis sp. (P73832), Synechocystis sp.
brenda
Obst, M.; Oppermann-Sanio, F.B.; Luftmann, H.; Steinbchel, A.
Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI
J. Biol. Chem.
277
25096-25105
2002
Pseudomonas anguilliseptica (Q8KQN8), Pseudomonas anguilliseptica, Pseudomonas anguilliseptica BI (Q8KQN8)
brenda
Fueser, G.; Steinbuechel, A.
Analysis of genome sequences for genes of cyanophycin metabolism: identifying putative cyanophycin metabolizing prokaryotes
Macromol. Biosci.
7
278-296
2007
Cupriavidus necator, Caulobacter vibrioides, Chloroflexus aurantiacus, Clostridium beijerinckii, Clostridium botulinum, Clostridium perfringens, Clostridium tetani, Acetivibrio thermocellus, Desulfitobacterium hafniense, Francisella tularensis, Mycobacterium marinum, Nostoc punctiforme, Nostoc sp., Rubrivivax gelatinosus, Synechococcus sp., Syntrophomonas wolfei, Thermoanaerobacter ethanolicus, Gloeobacter violaceus, Thermosynechococcus vestitus, Trichodesmium erythraeum, Chromohalobacter salexigens, Colwellia psychrerythraea, Acinetobacter baylyi, Crocosphaera watsonii, Gemmata obscuriglobus, Janibacter sp., Polaromonas sp., Trichormus variabilis (O86108), Pseudomonas anguilliseptica (Q8KQN8), Caldanaerobacter subterraneus subsp. tengcongensis (Q8R6L6), Chromohalobacter salexigens DSM 3043, Clostridium beijerinckii NCIMB 8052, Clostridium tetani E88, Syntrophomonas wolfei Goettingen, Chloroflexus aurantiacus J-10-fl, Pseudomonas anguilliseptica BI (Q8KQN8), Caulobacter vibrioides CB15, Crocosphaera watsonii WH 8501, Desulfitobacterium hafniense DCB-2, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Rubrivivax gelatinosus PM1
brenda
Sallam, A.; Kast, A.; Przybilla, S.; Meiswinkel, T.; Steinbchel, A.
Biotechnological process for production of beta-dipeptides from cyanophycin on a technical scale and its optimization
Appl. Environ. Microbiol.
75
29-38
2009
Pseudomonas alcaligenes, Pseudomonas alcaligenes DIP1
brenda
Law, A.M.; Lai, S.W.; Tavares, J.; Kimber, M.S.
The structural basis of beta-peptide-specific cleavage by the serine protease cyanophycinase
J. Mol. Biol.
392
393-404
2009
Synechocystis sp. PCC 6803 (P73832)
brenda
Burnat, M.; Herrero, A.; Flores, E.
Compartmentalized cyanophycin metabolism in the diazotrophic filaments of a heterocyst-forming cyanobacterium
Proc. Natl. Acad. Sci. USA
111
3823-3828
2014
Nostoc sp., Nostoc sp. PCC 7120
brenda
Frommeyer, M.; Bergander, K.; Steinbuechel, A.
Guanidination of soluble lysine-rich cyanophycin yields a homoarginine-containing polyamide
Appl. Environ. Microbiol.
80
2381-2389
2014
Pseudomonas alcaligenes (G8HR04), Pseudomonas alcaligenes, Pseudomonas alcaligenes DIP1 (G8HR04)
brenda
Liu, H.; Ray, W.K.; Helm, R.F.; Popham, D.L.; Melville, S.B.
Analysis of the spore membrane proteome in Clostridium perfringens implicates cyanophycin in spore assembly
J. Bacteriol.
198
1773-1782
2016
Clostridium perfringens (Q0SQW9), Clostridium perfringens, Clostridium perfringens SM101 (Q0SQW9)
brenda
Nausch, H.; Broer, I.
Cyanophycinase CphE from P. alcaligenes produced in different compartments of N. benthamiana degrades high amounts of cyanophycin in plant extracts
Appl. Microbiol. Biotechnol.
101
2397-2413
2017
Pseudomonas alcaligenes (G8HR04), Pseudomonas alcaligenes
brenda
Ponndorf, D.; Ehmke, S.; Walliser, B.; Thoss, K.; Unger, C.; Goers, S.; Das, G.; Metges, C.C.; Broer, I.; Nausch, H.
Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine
Plant Biotechnol. J.
15
605-613
2017
Thermosynechococcus vestitus (P0C8P3)
brenda
Ponndorf, D.; Broer, I.; Nausch, H.
Expression of CphB- and CphE-type cyanophycinases in cyanophycin-producing tobacco and comparison of their ability to degrade cyanophycin in plant and plant extracts
Transgenic Res.
26
491-499
2017
Pseudomonas alcaligenes (G8HR04), Thermosynechococcus vestitus (P0C8P3)
brenda