Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
2,5-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,5-dinitrophenol + D-glucose 6-phosphate
-
-
-
-
?
2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
2-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
2-nitrophenyl beta-D-galactoside 6-phosphate + H2O
2-nitrophenol + beta-D-galactose 6-phosphate
-
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-glucose 6-phosphate
3,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
3,4-dinitrophenol + D-glucose 6-phosphate
-
-
-
-
?
3,5-dichlorophenyl 6-phospho-beta-D-glucoside + H2O
3,5-dichlorophenol + D-glucose 6-phosphate
-
-
-
-
?
3-nitrophenyl 6-phospho-beta-D-glucoside + H2O
3-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-chloro-2-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-cyanophenyl 6-phospho-beta-D-glucoside + H2O
4-cyanophenol + D-glucose 6-phosphate
-
-
-
-
?
4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside + H2O
?
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O
4-methylumbelliferone + D-glucose 6-phosphate
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + D-glucose
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
strains expressing the Bgl isoform Val204 show a lower hydrolysing activity on 4-nitrophenyl-beta-D-glucopyranoside when compared to strains that possess the Bgl isoform Ala204
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucopyranose 6-phosphate
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
4-nitrothiophenyl beta-D-glucoside 6-phosphate + H2O
4-nitrothiophenol + beta-D-glucose 6-phosphate
-
-
-
-
?
4-tert-butylphenyl 6-phospho-beta-D-glucoside + H2O
4-tert-butylphenol + D-glucose 6-phosphate
-
-
-
-
?
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O
D-glucose + D-glucose 6-phosphate
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
cellobiitol monophosphate + H2O
?
-
-
-
-
?
cellobiose 6-phosphate + H2O
beta-D-glucose + glucose 6-phosphate
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
esculin 6-phosphate + H2O
?
-
-
-
-
?
gentiobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
lactose 6-phosphate + H2O
beta-D-galactose-6-phosphate + D-glucose
laminaribiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
?
methyl beta-glucoside 6-phosphate + H2O
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
o-nitrophenyl-beta-D-galactose + H2O
o-nitrophenol + D-galactose
-
very low activity
-
-
?
p-nitrophenyl 6-phospho-beta-D-thioglucoside + H2O
p-nitrophenol + 6-phospho-beta-D-thioglucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + D-glucose
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucose + H2O
p-nitrophenol + D-glucose
-
very low activity
-
-
?
p-nitrothiophenyl beta-glucoside 6-phosphate + H2O
?
-
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
sophorose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
additional information
?
-
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
-
high activity
-
-
?
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
-
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
?
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
gentiobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
gentiobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
gentiobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
lactose 6-phosphate + H2O
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
lactose 6-phosphate + H2O
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
?
-
-
-
-
?
methyl beta-D-glucoside monophosphate + H2O
?
-
-
-
-
?
methyl beta-glucoside 6-phosphate + H2O
?
-
-
-
-
?
methyl beta-glucoside 6-phosphate + H2O
?
-
-
-
?
methyl beta-glucoside 6-phosphate + H2O
?
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
isoenzyme B
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
-
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
-
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
isoenzyme B
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
-
?
additional information
?
-
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
?
additional information
?
-
-
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
?
additional information
?
-
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
?
additional information
?
-
-
expression of P-beta-glc induced by lactose is characteristic of Lactobacillus gasseri. Because this lactic acid bacterium is a major inhabitant of the human intestine, this enzyme is a key glycosidase involved in lactose utilization
-
-
?
additional information
?
-
the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85
-
-
?
additional information
?
-
-
the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85
-
-
?
additional information
?
-
the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85
-
-
?
additional information
?
-
single nucleotide polymorphism in the bgl gene coding for the 6-phospho-beta-glucosidase is one possible cause of the difference in bacterial virulence among strains of Mycoplasma mycoides subsp. mycoides SC. Bgl does not act as a direct virulence factor, but strains possessing the Bgl isoform Val204 with low hydrolysing activity are more prone to survive in environments that contain high levels of beta-D-glucosides, thus contributing in some extent to mycoplasmaemia
-
-
?
additional information
?
-
-
the enzyme is not active against non-phosphorylated beta-glucosides
-
-
?
additional information
?
-
no activity with beta-(1,4)-thiocellobiose 6-phosphate
-
-
?
additional information
?
-
-
no activity with beta-(1,4)-thiocellobiose 6-phosphate
-
-
?
additional information
?
-
-
no activity with 2-nitrophenyl beta-D-galactopyranoside, 4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide, 4-methylumbelliferyl beta-D-glucopyranoside, 4-methylumbelliferyl alpha-D-glucopyranoside, 2-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucoside 6-phosphate, 4-nitrophenyl alpha-D-mannoside 6-phosphate, and 4-nitrophenyl alpha-D-galactoside 6-phosphate
-
-
?
additional information
?
-
the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine
-
-
?
additional information
?
-
-
the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine
-
-
?
additional information
?
-
the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine
-
-
?
additional information
?
-
the enzyme shows no beta-glucosidase activity, EC 3.2.1.21
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0444
2,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.0166
2,5-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.0174
2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.25
2-nitrophenyl beta-D-galactoside 6-phosphate
-
isoenzyme B
-
0.66 - 0.97
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate
0.0314
3,4-dinitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.0414
3,5-dichlorophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.0729
3-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.015
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.0319 - 0.0454
4-cyanophenyl 6-phospho-beta-D-glucoside
0.3
4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside
-
0.0092
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate
-
-
0.048
4-nitrophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.73 - 1.416
4-nitrophenyl 6-phospho-beta-glucoside
0.44
4-nitrophenyl beta-D-glucopyranoside 6-phosphate
-
0.478 - 4.8
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
0.11
4-nitrothiophenyl beta-D-glucoside 6-phosphate
-
isoenzyme B
-
0.0583
4-tert-butylphenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.23 - 12.5
6-O-(6-O-phosphono-beta-D-glucopyranosyl)-beta-D-glucopyranose
0.0977 - 0.5
arbutin 6-phosphate
0.23 - 0.5
cellobiitol monophosphate
0.1823 - 1.515
cellobiose 6-phosphate
0.24 - 0.3355
gentiobiose 6-phosphate
0.0875
laminaribiose 6-phosphate
-
-
0.19 - 2.22
methyl beta-D-glucoside 6-phosphate
0.23 - 0.5
methyl beta-D-glucoside monophosphate
-
-
0.27
methyl beta-D-glucoside phosphate
-
-
0.1384
methyl-beta-D-glucoside 6-phosphate
-
-
0.206
p-nitrophenyl 6-phospho-beta-D-thioglucoside
pH 7.5, 50°C
0.21
p-nitrophenyl-beta-D-glucopyranoside
-
pH 7.0, 40°C
0.0291 - 0.18
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
0.33
p-nitrophenyl-D-beta-D-glucopyranoside 6-phosphate
-
isoenzyme A
0.23 - 0.6
phenyl beta-D-glucoside 6-phosphate
0.0886 - 0.51
Salicin 6-phosphate
0.1212
sophorose 6-phosphate
-
-
additional information
additional information
-
0.66
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.0, 40°C
0.97
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 6.0, 50°C
0.0319
4-cyanophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.0454
4-cyanophenyl 6-phospho-beta-D-glucoside
-
pH 7.5, 50°C
0.73
4-nitrophenyl 6-phospho-beta-glucoside
at pH 6.0 and 37°C
1.416
4-nitrophenyl 6-phospho-beta-glucoside
-
at pH 7.5 and 37°C
0.478
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.5, 37°C, recombinant His-tagged wild-type enzyme
0.512
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.5, 37°C, recombinant His-tagged mutant M423A
0.598
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.5, 37°C, recombinant His-tagged mutant Y126F
0.73
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 6.0, 37°C
4.8
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.0, 37°C
0.23 - 0.5
6-O-(6-O-phosphono-beta-D-glucopyranosyl)-beta-D-glucopyranose
-
-
12.5
6-O-(6-O-phosphono-beta-D-glucopyranosyl)-beta-D-glucopyranose
-
0.0977
arbutin 6-phosphate
-
-
0.23 - 0.5
arbutin 6-phosphate
-
-
0.31
arbutin 6-phosphate
-
-
0.35
arbutin 6-phosphate
-
0.23 - 0.5
cellobiitol monophosphate
-
-
0.25
cellobiitol monophosphate
-
-
0.1823
cellobiose 6-phosphate
-
-
0.23
cellobiose 6-phosphate
-
-
0.23 - 0.5
cellobiose 6-phosphate
-
-
1.135
cellobiose 6-phosphate
pH 7.5, 37°C, recombinant His-tagged wild-type enzyme
1.3
cellobiose 6-phosphate
-
1.32
cellobiose 6-phosphate
pH 7.5, 37°C, recombinant His-tagged mutant M423A
1.515
cellobiose 6-phosphate
pH 7.5, 37°C, recombinant His-tagged mutant Y126F
0.24
gentiobiose 6-phosphate
-
-
0.3355
gentiobiose 6-phosphate
-
-
0.19
methyl beta-D-glucoside 6-phosphate
-
isoenzyme B
0.28
methyl beta-D-glucoside 6-phosphate
-
isoenzyme A
2.22
methyl beta-D-glucoside 6-phosphate
-
0.0291
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
0.041
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.5
0.041
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
pH 7.5, 50°C
0.18
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
isoenzyme B
0.23 - 0.5
phenyl beta-D-glucoside 6-phosphate
-
-
0.46
phenyl beta-D-glucoside 6-phosphate
-
isoenzyme B
0.5
phenyl beta-D-glucoside 6-phosphate
-
-
0.6
phenyl beta-D-glucoside 6-phosphate
-
isoenzyme A
0.0886
Salicin 6-phosphate
-
-
0.23 - 0.5
Salicin 6-phosphate
-
-
0.44
Salicin 6-phosphate
-
0.5
Salicin 6-phosphate
-
-
0.51
Salicin 6-phosphate
-
isoenzyme B
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
malfunction
-
an enzyme deletion mutant shows reduced 6-phospho-glucosidase activity and is attenuated in growth on cellobiose and hyaluronic acid compared to the growth of wild type. Mutant-infected mice survive significantly longer than the wild-type-infected cohort, and the colony counts of the mutant are lower than those of the wild type in the lungs. The enzyme deletion mutant is also severely impaired in attachment to an abiotic surface
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1
evolution
the enzyme belongs to the glycosyl hydrolase family 1, GH1. Members of the GH-1 family share a common catalytic mechanism and exhibit similar structural folds, including a (beta/alpha)8 TIM-barrel
evolution
-
the enzyme belongs to the glycosyl hydrolase family 1, GH1
-
metabolism
the enzyme is involved in the metabolic pathway of lactose in Lactobacillus gasseri, overview
metabolism
-
the enzyme is involved in the metabolic pathway of lactose in Lactobacillus gasseri, overview
-
physiological function
the enzyme SPy1599 is involved in the intracellular degradation of 6-phosphoglycosides, which are likely to originate from import through one of the organism's many phosphoenolpyruvate phosphotransfer systems
physiological function
-
the enzyme SPy1599 is involved in the intracellular degradation of 6-phosphoglycosides, which are likely to originate from import through one of the organism's many phosphoenolpyruvate phosphotransfer systems
-
additional information
conserved residues S427, Lys435, and Tyr437 act as gatekeepers in a phosphate-binding loop and play important roles in phosphate recognition, homology structure modelling, overview
additional information
structure comparisons, active center and substrate specificity, overview
additional information
-
structure comparisons, active center and substrate specificity, overview
additional information
the overall structure of enzyme BglA-2 adopts a typical (beta/alpha)8 TIM-barrel, with the active site located at the center of the convex surface of the beta-barrel. Residues Tyr126, Tyr303, and Trp338, at subsite +1 of BglA-2 determine substrate specificity with respect to 1,4-linked 6-phospho-beta-glucosides. Residues Ser424, Lys430, and Tyr432 of BglA-2 play important roles in the hydrolytic selectivity toward phosphorylated rather than non-phosphorylated compounds, comparative structural analysis. Tryptophan versus a methionine/alanine residue at subsite -1 may contribute to the catalytic and substrate selectivity with respect to structurally similar 6-phospho-beta-galactosidases and 6-phospho-beta-glucosidases assigned to the GH-1 family
additional information
-
the overall structure of enzyme BglA-2 adopts a typical (beta/alpha)8 TIM-barrel, with the active site located at the center of the convex surface of the beta-barrel. Residues Tyr126, Tyr303, and Trp338, at subsite +1 of BglA-2 determine substrate specificity with respect to 1,4-linked 6-phospho-beta-glucosides. Residues Ser424, Lys430, and Tyr432 of BglA-2 play important roles in the hydrolytic selectivity toward phosphorylated rather than non-phosphorylated compounds, comparative structural analysis. Tryptophan versus a methionine/alanine residue at subsite -1 may contribute to the catalytic and substrate selectivity with respect to structurally similar 6-phospho-beta-galactosidases and 6-phospho-beta-glucosidases assigned to the GH-1 family
additional information
-
structure comparisons, active center and substrate specificity, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
E173S
site-directed mutagenesis
E173S/I174V
site-directed mutagenesis
I174V
site-directed mutagenesis
E173S
-
site-directed mutagenesis
-
E173S/I174V
-
site-directed mutagenesis
-
I174V
-
site-directed mutagenesis
-
C195G
the mutant shows significantly enhanced activity towards salicin compared to the wild type enzyme
W433A
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
W433M
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
E170Q
-
catalytic mutant
-
W433A
-
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
-
W433M
-
the mutation shifts the enzyme specificity from dual activity to a significant preference toward 6-phospho-beta-glucosidase activity
-
E171A
site-directed mutagenesis, inactive mutant
E171Q
site-directed mutagenesis, inactive mutant
E364A
site-directed mutagenesis, inactive mutant
E364Q
site-directed mutagenesis, inactive mutant
K430A
site-directed mutagenesis, inactive mutant
M423A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
S424A
site-directed mutagenesis, inactive mutant
W338A
site-directed mutagenesis, inactive mutant
Y126A
site-directed mutagenesis, inactive mutant
Y126F
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y303A
site-directed mutagenesis, inactive mutant
Y303F
site-directed mutagenesis, inactive mutant
Y432F
site-directed mutagenesis, inactive mutant
E179A
site-directed mutagenesis, inactive mutant
E372A
site-directed mutagenesis, inactive mutant
K435L
site-directed mutagenesis, inactive mutant
S427A
site-directed mutagenesis, the mutant shows slightly decreased activity but substrate affinities compared to the wild-type enzyme
S427C
site-directed mutagenesis, the mutant shows increased activity but substrate affinities compared to the wild-type enzyme
S427D
site-directed mutagenesis, almost inactive mutant
S427E
site-directed mutagenesis, almost inactive mutant
W420A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
W420F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Y311F
site-directed mutagenesis, inactive mutant
Y437F
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Schnetz, K.; Toloczyki, C.; Rak, B.
beta-Glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes
J. Bacteriol.
169
2579-2590
1987
Escherichia coli
brenda
Anderson, R.L.; Palmer, R.E.
Phosphocellobiase
Methods Enzymol.
42 C
494-497
1975
Klebsiella aerogenes
brenda
Wilson, G.; Fox, C.F.
The beta-glucoside system of Escherichia coli. IV. Purification and properties of phospho-beta-glucosidases A and B
J. Biol. Chem.
249
5586-5598
1974
Escherichia coli
brenda
Palmer, R.E.; Anderson, R.L.
Cellobiose metabolism in Aerobacter aerogenes. 3. Cleavage of cellobiose monophosphate by a phospho-beta-glucosidase
J. Biol. Chem.
247
3420-3423
1972
Klebsiella aerogenes
brenda
Prasad, I.; Young, B.; Schaefler, S.
Genetic determination of the constitutive biosynthesis of phospho-beta-glucosidase A in Escherichia coli K-12
J. Bacteriol.
114
909-915
1973
Escherichia coli
brenda
Thompson, J.; Robrish, S.A.; Bouma, C.L.; Freedberg, D.I.; Folk, J.E.
Phospho-beta-glucosidase from Fusobacterium mortiferum: Purification, clonig, and inactivation by 6-phosphoglucono-sigma-lactone
J. Bacteriol.
179
1636-1645
1997
Fusobacterium mortiferum
brenda
Thompson, J.; Ruvinov, S.B.; Freedberg, D.I.; Hall, B.G.
Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: Characterization and Assignment to the unsual family 4 of glycosylhydrolases
J. Bacteriol.
181
7339-7345
1999
Escherichia coli (P17411), Escherichia coli
brenda
Simons, G.; Nijhuis, M.; de Vos, W.
Integration and gene replacement in the Lactococcus lactis lac operon: Induction of a cryptic phospho-beta-glucosidase in LacG-deficient strains
J. Bacteriol.
175
5168-5175
1993
Lactococcus lactis
brenda
Yip, V.L.Y.; Varrot, A.; Davies, G.J.; Rajan, S.S.; Yang, X.; Thompson, J.; Anderson, W.F.; Withers, S.G.
An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima
J. Am. Chem. Soc.
126
8354-8355
2004
Thermotoga maritima (Q9X108)
brenda
Weber, B.A.; Klein, J.R.; Henrich, B.
Expression of the phospho-beta-glycosidase ArbZ from Lactobacillus delbrueckii subsp. lactis in Lactobacillus helveticus: substrate induction and catabolite repression
Microbiology
146
1941-1948
2000
Lactobacillus delbrueckii
brenda
Varrot, A.; Yip, V.L.; Li, Y.; Rajan, S.S.; Yang, X.; Anderson, W.F.; Thompson, J.; Withers, S.G.; Davies, G.J.
NAD+ and Metal-ion Dependent Hydrolysis by Family 4 Glycosidases: Structural Insight into Specificity for Phospho-b-D-glucosides
J. Mol. Biol.
346
423-435
2005
Thermotoga maritima (Q9X108), Thermotoga maritima
brenda
An, C.L.; Lim, W.J.; Hong, S.Y.; Shin, E.C.; Kim, M.K.; Lee, J.R.; Park, S.R.; Woo, J.G.; Lim, Y.P.; Yun, H.D.
Structural and biochemical analysis of the asc operon encoding 6-phospho-beta-glucosidase in Pectobacterium carotovorum subsp. carotovorum LY34
Res. Microbiol.
156
145-153
2005
Pectobacterium carotovorum
brenda
Yip, V.L.; Withers, S.G.
Mechanistic analysis of the unusual redox-elimination sequence employed by Thermotoga maritima BglT: a 6-phospho-beta-glucosidase from glycoside hydrolase family 4
Biochemistry
45
571-580
2006
Thermotoga maritima
brenda
Vilei, E.M.; Correia, I.; Ferronha, M.H.; Bischof, D.F.; Frey, J.
beta-D-Glucoside utilization by Mycoplasma mycoides subsp. mycoides SC: possible involvement in the control of cytotoxicity towards bovine lung cells
BMC Microbiol.
7
31
2007
Mycoplasma mycoides (Q6MSD6)
brenda
Honda, H.; Kataoka, F.; Nagaoka, S.; Kawai, Y.; Kitazawa, H.; Itoh, H.; Kimura, K.; Taketomo, N.; Yamazaki, Y.; Tateno, Y.; Saito, T.
beta-Galactosidase, phospho-beta-galactosidase and phospho-beta-glucosidase activities in Lactobacilli strains isolated from human faeces
Lett. Appl. Microbiol.
45
461-466
2007
Lactobacillus acidophilus, Lacticaseibacillus casei, Limosilactobacillus fermentum, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus amylovorus, Limosilactobacillus mucosae, Lactobacillus acidophilus JCM 1132T
brenda
Hall, B.G.; Pikis, A.; Thompson, J.
Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations
Mol. Biol. Evol.
26
2487-2497
2009
Erwinia rhapontici (Q9AI65), Erwinia rhapontici, Erwinia rhapontici DSM 448 (Q9AI65)
brenda
Capaldo, A.; Walker, M.; Ford, C.; Jiranek, V.
beta-Glucoside metabolism in Oenococcus oeni: Cloning and characterization of the phospho-beta-glucosidase CelD
J. Mol. Catal. B
69
27-34
2011
Oenococcus oeni
-
brenda
Stepper, J.; Dabin, J.; Eklof, J.M.; Thongpoo, P.; Kongsaeree, P.; Taylor, E.J.; Turkenburg, J.P.; Brumer, H.; Davies, G.J.
Structure and activity of the Streptococcus pyogenes family GH1 6-phospho-beta-glucosidase SPy1599
Acta Crystallogr. Sect. D
69
16-23
2013
Streptococcus pyogenes (Q1JK37), Streptococcus pyogenes, Streptococcus pyogenes M1 GAS SF370 (Q1JK37)
brenda
Yang, C.; Niu, Y.; Li, C.; Zhu, D.; Wang, W.; Liu, X.; Cheng, B.; Ma, C.; Xu, P.
Characterization of a novel metagenome-derived 6-phospho-beta-glucosidase from black liquor sediment
Appl. Environ. Microbiol.
79
2121-2127
2013
uncultured organism (L7Y2Z6)
brenda
Yu, W.L.; Jiang, Y.L.; Pikis, A.; Cheng, W.; Bai, X.H.; Ren, Y.M.; Thompson, J.; Zhou, C.Z.; Chen, Y.
Structural insights into the substrate specificity of a 6-phospho-beta-glucosidase BglA-2 from Streptococcus pneumoniae TIGR4
J. Biol. Chem.
288
14949-14958
2013
Streptococcus pneumoniae (A0A0H2UP35), Streptococcus pneumoniae
brenda
Honda, H.; Nagaoka, S.; Kawai, Y.; Kemperman, R.; Kok, J.; Yamazaki, Y.; Tateno, Y.; Kitazawa, H.; Saito, T.
Purification and characterization of two phospho-?-galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323
J. Gen. Appl. Microbiol.
58
11-17
2012
Lactobacillus gasseri (O06532), Lactobacillus gasseri, Lactobacillus gasseri ATCC 33323 (O06532)
brenda
Lansky, S.; Zehavi, A.; Belrhali, H.; Shoham, Y.; Shoham, G.
Structural basis for enzyme bifunctionality - the case of Gan1D from Geobacillus stearothermophilus
FEBS J.
284
3931-3953
2017
Geobacillus stearothermophilus (W8QF82), Geobacillus stearothermophilus T-1 (W8QF82)
brenda
Kwan, D.H.; Jin, Y.; Jiang, J.; Chen, H.M.; Koetzler, M.P.; Overkleeft, H.S.; Davies, G.J.; Withers, S.G.
Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases
FEBS Lett.
590
461-468
2016
Streptococcus pyogenes (Q99YP9), Streptococcus pyogenes M1 (Q99YP9)
brenda
Terra, V.S.; Zhi, X.; Kahya, H.F.; Andrew, P.W.; Yesilkaya, H.
Pneumococcal 6-phospho-beta-glucosidase (BglA3) is involved in virulence and nutrient metabolism
Infect. Immun.
84
286-292
2016
Streptococcus pneumoniae
brenda
Zangoui, P.; Vashishtha, K.; Mahadevan, S.
Evolution of aromatic beta-glucoside utilization by successive mutational steps in Escherichia coli
J. Bacteriol.
197
710-716
2015
Escherichia coli (Q46829)
brenda
Yang, S.; Chen, Q.; Li, Y.; Xing, Y.; He, L.; Liu, S.
Coloring and bioinformatic analysis of the phospho-beta-glucosidase gene in Oenococcus oeni
J. Chin. Inst. Food Sci. Technol.
17
197-205
2017
Oenococcus oeni
-
brenda