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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans
Synonyms
glucosidase ii, mutanase rm1,
more
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exo-1,3-alpha-glucanase
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-
-
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alpha-(1->3)-glucanase
-
-
alpha-(1->3)-glucanase
-
-
-
exomutanase
-
-
MalA
-
mutanase
-
Mutanase RM1
E16590
-
Saci1160
locus name
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Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans
-
-
-
-
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hydrolysis of O-glycosyl bond
hydrolysis
E16590
of alpha-1,3-glucan, C-terminal domain with catalytic activity and low mutan-binding activity, N-terminal domain with mutan-binding activity
hydrolysis
-
of alpha-1,3-glucan, C-terminal domain with catalytic activity and low mutan-binding activity, N-terminal domain with mutan-binding activity
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hydrolysis of O-glycosyl bond
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hydrolysis of O-glycosyl bond
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hydrolysis of O-glycosyl bond
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hydrolysis of O-glycosyl bond
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hydrolysis of O-glycosyl bond
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-
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3-alpha-D-glucan 3-glucohydrolase
Does not act on nigeran.
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alpha-1,3-glucan + H2O
alpha-D-glucose
alpha-1,3-glucans + H2O
alpha-D-glucose
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exo-wise hydrolysis from non-reducing ends
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-
?
alpha-1,3-mutan + H2O
alpha-D-glucose
beta-glucan:chitin + H2O
?
nigerose + H2O
alpha-D-glucose + D-glucose
pseudonigeran + H2O
alpha-D-glucose
sucrose + H2O
D-glucose + D-fructose
additional information
?
-
alpha-1,3-glucan + H2O
alpha-D-glucose
-
-
-
?
alpha-1,3-glucan + H2O
alpha-D-glucose
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-
-
-
?
alpha-1,3-glucan + H2O
alpha-D-glucose
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-
-
-
?
alpha-1,3-glucan + H2O
alpha-D-glucose
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-
-
-
?
alpha-1,3-glucan + H2O
alpha-D-glucose
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-
-
-
?
alpha-1,3-glucan + H2O
alpha-D-glucose
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-
-
-
?
alpha-1,3-mutan + H2O
alpha-D-glucose
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-
-
?
alpha-1,3-mutan + H2O
alpha-D-glucose
involved in cell wall synthesis and sexual development
-
-
?
alpha-1,3-mutan + H2O
alpha-D-glucose
-
the mutanase extensively hydrolyzes streptococcal mutan, giving 23% of saccharification, and 83% of solubilization of glucan after 6 h
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-
?
alpha-1,3-mutan + H2O
alpha-D-glucose
-
the mutanase extensively hydrolyzes streptococcal mutan, giving 23% of saccharification, and 83% of solubilization of glucan after 6 h
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-
?
alpha-1,3-mutan + H2O
alpha-D-glucose
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-
-
-
?
alpha-1,3-mutan + H2O
alpha-D-glucose
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-
-
-
?
beta-glucan:chitin + H2O
?
-
different activities with substrates from cell walls of different microorganisms
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-
?
beta-glucan:chitin + H2O
?
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different activities with substrates from cell walls of different microorganisms
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-
?
mutan
glucan
E16590
-
low-molecular-weight
-
?
mutan
glucan
E16590
degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
-
-
?
mutan
glucan
E16590
-
low-molecular-weight
-
?
mutan
glucan
E16590
degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
-
-
?
nigerose + H2O
alpha-D-glucose + D-glucose
i.e. alpha-D-Glc-(1->3)-D-Glc. The multifunctional enzyme also catalyzes the hydrolysis of terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,6-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.10 hydrolase)
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-
?
nigerose + H2O
alpha-D-glucose + D-glucose
i.e. alpha-D-Glc-(1->3)-D-Glc. The multifunctional enzyme also catalyzes the hydrolysis of terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,6-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.10 hydrolase)
-
-
?
pseudonigeran + H2O
alpha-D-glucose
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-
-
-
?
pseudonigeran + H2O
alpha-D-glucose
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
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-
-
-
?
additional information
?
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-
the mutanase also degrades alpha-1,3-polymers of biofilms, formed in vitro by Streptococcus mutans, even after only 3 min of contact. Maximum dissociation of adherent biofilm (64%) occurs at the highest enzyme concentration (2 units/ml)
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-
?
additional information
?
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the mutanase also degrades alpha-1,3-polymers of biofilms, formed in vitro by Streptococcus mutans, even after only 3 min of contact. Maximum dissociation of adherent biofilm (64%) occurs at the highest enzyme concentration (2 units/ml)
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-
?
additional information
?
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lytic and antifungal activity against fungal plant panthogens
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?
additional information
?
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no activity with beta-glucan or alpha-1,4 or alpha-1,6 linked glucose chains
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-
?
additional information
?
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the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans
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-
?
additional information
?
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alpha-(1,3)-endo-glucanase activity with lyophilized Laetiporus sulphureus cell walls prepared from fruiting bodies, also glucans are isolated from fruiting bodies of Pleurotus precoce, Pleurotus djamor, Pleurotus citrinopileatus, Pleurotus eryngii, Piptoporus betulinus, Laetiporus sulphureus and Hericium erinaceus, and from mycelia of Aspergillus fumigatus CCM F-614, Aspergillus wentii CIM 449, and Aspergillus nidulans DIM AN1, and with dextranase-pretreated mutan synthesized from sucrose using cell-free glucosyltransferases of Streptococci, substrate specificity, overview
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-
?
additional information
?
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the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans
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-
?
additional information
?
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-
alpha-(1,3)-endo-glucanase activity with lyophilized Laetiporus sulphureus cell walls prepared from fruiting bodies, also glucans are isolated from fruiting bodies of Pleurotus precoce, Pleurotus djamor, Pleurotus citrinopileatus, Pleurotus eryngii, Piptoporus betulinus, Laetiporus sulphureus and Hericium erinaceus, and from mycelia of Aspergillus fumigatus CCM F-614, Aspergillus wentii CIM 449, and Aspergillus nidulans DIM AN1, and with dextranase-pretreated mutan synthesized from sucrose using cell-free glucosyltransferases of Streptococci, substrate specificity, overview
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-
?
additional information
?
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lytic and antifungal activity against fungal plant panthogens
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-
?
additional information
?
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no activity with beta-glucan or alpha-1,4 or alpha-1,6 linked glucose chains
-
-
?
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alpha-1,3-mutan + H2O
alpha-D-glucose
involved in cell wall synthesis and sexual development
-
-
?
additional information
?
-
mutan
glucan
E16590
degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
-
-
?
mutan
glucan
E16590
degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
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-
?
additional information
?
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lytic and antifungal activity against fungal plant panthogens
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-
?
additional information
?
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the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans
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-
?
additional information
?
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the enzyme preferentially catalyzes the hydrolysis of various streptococcal mutans and fungal alpha-(1->3)-glucans
-
-
?
additional information
?
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-
lytic and antifungal activity against fungal plant panthogens
-
-
?
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Mg2+
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activates slightly
additional information
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no or poor effects by Ba2+, K+, and Fe2+
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6-epicastanospermine
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poor inhibitor
Ca2+
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moderate inhibition at 1 mM
Co2+
-
moderate inhibition at 1 mM
Cu2+
-
moderate inhibition at 1 mM
Mn2+
-
moderate inhibition at 1 mM
Sn2+
-
moderate inhibition at 1 mM
Zn2+
-
moderate inhibition at 1 mM
Fe3+
-
-
Fe3+
-
moderate inhibition at 1 mM
Hg2+
-
-
Hg2+
-
complete inhibition at 1 mM
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alpha-1,3-glucan
-
streptococcal mutan, induction
cell wall material from fruiting bodies of Laetiporus sulphureus
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induction
-
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Carcinogenesis
Glucosidase II beta subunit (GluII?) plays a role in autophagy and apoptosis regulation in lung carcinoma cells in a p53-dependent manner.
Carcinoma
Glucosidase II beta subunit (GluII?) plays a role in autophagy and apoptosis regulation in lung carcinoma cells in a p53-dependent manner.
Carcinoma, Hepatocellular
Endoplasmic reticulum stress underlying the pro-apoptotic effect of epigallocatechin gallate in mouse hepatoma cells.
Carcinoma, Hepatocellular
Glucosidase II, a protein of the endoplasmic reticulum with high mannose oligosaccharide chains and a rapid turnover.
Carcinoma, Hepatocellular
[Effect of green tea flavanols on the functions of the endoplasmic reticulum.]
Dental Plaque
Purification and properties of an ?-(1 ? 3)-glucanase (EC 3.2.1.84) from Trichoderma harzianum and its use for reduction of artificial dental plaque accumulation.
Diabetes Mellitus, Type 2
Metabolic syndrome perturbs deglucosylation and reglucosylation in the glycoprotein folding cycle.
Infections
Antibody-independent, complement-mediated enhancement of HIV-1 infection by mannosidase I and II inhibitors.
Infections
Proteomics analysis reveals novel host molecular mechanisms associated with thermotherapy of 'Ca. Liberibacter asiaticus'-infected citrus plants.
Liver Diseases
Molecular characterization of hepatocystin, the protein that is defective in autosomal dominant polycystic liver disease.
Liver Diseases
Mutations in SEC63 cause autosomal dominant polycystic liver disease.
Liver Diseases
N-glycosylation determines the abundance of the transient receptor potential channel TRPP2.
Liver Diseases
Polycystic liver disease is a disorder of cotranslational protein processing.
Lung Neoplasms
Knockout of glucosidase II beta subunit inhibits growth and metastatic potential of lung cancer cells by inhibiting receptor tyrosine kinase activities.
Lymphoma
A lectin-resistant mouse lymphoma cell line is deficient in glucosidase II, a glycoprotein-processing enzyme.
Lymphoma
Expression of a cDNA encoding the glucose trimming enzyme glucosidase II in CHO cells and molecular characterization of the enzyme deficiency in a mutant mouse lymphoma cell line.
Lymphoma
Identification of a novel mechanism for the removal of glucose residues from high mannose-type oligosaccharides.
Lymphoma
Identity of neutral alpha-glucosidase AB and the glycoprotein processing enzyme glucosidase II. Biochemical and genetic studies.
Lymphoma
N-linked oligosaccharide processing enzyme glucosidase II produces 1,5-anhydrofructose as a side product.
Lymphoma
The effect of castanospermine on the oligosaccharide structures of glycoproteins from lymphoma cell lines.
Metabolic Syndrome
Metabolic syndrome perturbs deglucosylation and reglucosylation in the glycoprotein folding cycle.
Neoplasms
Glucosidase II exhibits similarity to the p53 tumor suppressor in regards to structure and behavior in response to stress signals: A potential novel cancer biomarker.
Neoplasms
Knockout of glucosidase II beta subunit inhibits growth and metastatic potential of lung cancer cells by inhibiting receptor tyrosine kinase activities.
Neoplasms
Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.
Obesity
Metabolic syndrome perturbs deglucosylation and reglucosylation in the glycoprotein folding cycle.
Polycystic Kidney Diseases
GANAB and PKD1 Variations in a 12 Years Old Female Patient With Early Onset of Autosomal Dominant Polycystic Kidney Disease.
Polycystic Kidney, Autosomal Dominant
GANAB and PKD1 Variations in a 12 Years Old Female Patient With Early Onset of Autosomal Dominant Polycystic Kidney Disease.
Vesicular Stomatitis
Identification of a novel mechanism for the removal of glucose residues from high mannose-type oligosaccharides.
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additional information
additional information
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Km is 0.73 mg/ml with lyophilized Laetiporus sulphureus cell walls, pH 5.5, 45°C
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7.1
pseudonigeran
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-
-
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0.178
-
after incubation for 1 h at 45°C, medium supplementation with 2.0% cell wall preparation of Laetiporus sulphureus
1.72
-
after incubation for 1 h at 45°C, medium supplementation with 0.4% cell wall preparation of Laetiporus sulphureus
115.6
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purified enzyme with lyophilized Laetiporus sulphureus cell walls, pH 5.5, 45°C
5.6
E16590
incubation at 35°C for 10 min, stop reaction by adding 0.4 ml of dinitrosalicylic acid solution, N-terminal domain with mutan-binding activity, C-terminal domain with mutanase activity and only low mutan-binding activity
additional information
E16590
the specific activity for degradation of Streptococcus mutans biofilms is 1.4 micromol/ml after incubation at 35°C at pH 6.0 for 3 min, degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
additional information
-
the specific activity for degradation of Streptococcus mutans biofilms is 1.4 micromol/ml after incubation at 35°C at pH 6.0 for 3 min, degradation of Streptococcus mutans biofilms only with structural integrity of the N-terminal Mutan-binding-domain and the C-terminal Mutanase-activity-domain separated by a characteristic sequence of proline and threonine repeats
additional information
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medium supplementation with more than 0.4% cell wall preparation results in lower specific activity
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4.5
-
-
5.5
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-
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4 - 7
E16590
80% of residual activity in comparison to the activity at ph 4.0
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55
-
-
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4.9
calculated from amino acid sequence
5.9
calculated from sequence
7.1
-
isoelectric focusing
7.5
-
basic chromatofocusing
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brenda
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brenda
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brenda
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brenda
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E16590
GenBank
brenda
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UniProt
brenda
-
UniProt
brenda
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-
-
brenda
sequence was found to be identical to previously published data
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-
brenda
rich in alpha-(1-3)-glucans
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brenda
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brenda
mung bean
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brenda
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brenda
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SwissProt
brenda
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E16590
GenBank
brenda
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brenda
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brenda
rich in alpha-(1-3)-glucans
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brenda
sequence was found to be identical to previously published data
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-
brenda
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brenda
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brenda
the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars
brenda
-
the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars
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brenda
the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 10fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, than during growth on other sugars
brenda
-
the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 10fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, than during growth on other sugars
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brenda
-
-
brenda
-
-
-
brenda
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brenda
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-
brenda
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brenda
E16590
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-
brenda
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-
-
-
brenda
-
-
-
brenda
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-
-
-
brenda
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-
-
-
brenda
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brenda
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brenda
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brenda
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PSL5_ARATH
921
0
104275
Swiss-Prot
Secretory Pathway (Reliability: 4 )
G7JC76_MEDTR
912
0
103720
TrEMBL
Secretory Pathway (Reliability: 5 )
A0A0B7IJZ5_9FLAO
816
0
91957
TrEMBL
-
A0A425HRL4_TOXGO
190
0
21703
TrEMBL
other Location (Reliability: 2 )
A0A5B6ZFQ5_DAVIN
454
0
52021
TrEMBL
Mitochondrion (Reliability: 5 )
D2BXL8_DICZ5
Dickeya zeae (strain Ech586)
791
0
91018
TrEMBL
-
A0A8B6E3Y6_MYTGA
933
0
106960
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A086KS74_TOXGO
846
1
94235
TrEMBL
Mitochondrion (Reliability: 4 )
M5BUR3_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
102
0
11560
TrEMBL
Secretory Pathway (Reliability: 5 )
M2X6J7_GALSU
945
1
109295
TrEMBL
Secretory Pathway (Reliability: 1 )
S7UN63_TOXGG
Toxoplasma gondii (strain ATCC 50853 / GT1)
1613
1
182942
TrEMBL
Mitochondrion (Reliability: 4 )
E3G2X8_ENTLS
Enterobacter lignolyticus (strain SCF1)
787
0
90294
TrEMBL
-
A0A422Q932_9TRYP
820
0
92359
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A139XSR7_TOXGO
425
0
48515
TrEMBL
other Location (Reliability: 2 )
A0A1Z5KGN8_FISSO
908
0
104425
TrEMBL
Secretory Pathway (Reliability: 2 )
U3TW29_9ENTR
245
0
28541
TrEMBL
-
A0A2I0A2R8_9ASPA
783
1
88756
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A1W2TVP5_ROSNE
979
1
110563
TrEMBL
Secretory Pathway (Reliability: 1 )
C6CFE6_DICC1
Dickeya chrysanthemi (strain Ech1591)
791
0
90941
TrEMBL
-
A0A086Q1J4_TOXGO
1440
1
162900
TrEMBL
Mitochondrion (Reliability: 4 )
A0A086LU00_TOXGO
205
0
24052
TrEMBL
other Location (Reliability: 3 )
A0A5B6ZHE6_DAVIN
903
0
103161
TrEMBL
Mitochondrion (Reliability: 5 )
A0A0B7HVX1_9FLAO
515
1
58143
TrEMBL
-
A0A0D2IUL4_9CHLO
112
0
13067
TrEMBL
Mitochondrion (Reliability: 3 )
I1DXM8_9GAMM
800
0
89677
TrEMBL
-
A0A139XSS1_TOXGO
658
0
73240
TrEMBL
Mitochondrion (Reliability: 5 )
A0A1Z5KG45_FISSO
28
0
3617
TrEMBL
other Location (Reliability: 2 )
A0A5B6ZIE0_DAVIN
645
0
74151
TrEMBL
other Location (Reliability: 2 )
A0A2P6RSU5_ROSCH
924
1
105245
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A0D2KEU3_9CHLO
248
0
26817
TrEMBL
other Location (Reliability: 2 )
A1L3J7_XENLA
933
1
106090
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A061ID46_CRIGR
966
1
109506
TrEMBL
Mitochondrion (Reliability: 5 )
A0A140N6E2_ECOBD
Escherichia coli (strain B / BL21-DE3)
793
0
92035
TrEMBL
-
F9YU50_CAPCC
Capnocytophaga canimorsus (strain 5)
1281
1
144581
TrEMBL
-
A0A0B7F6Z4_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
572
0
64313
TrEMBL
Secretory Pathway (Reliability: 3 )
K0KKJ4_WICCF
Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031 F-60-10)
914
0
104933
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A5B6ZEM0_DAVIN
472
0
53588
TrEMBL
Mitochondrion (Reliability: 5 )
A0A8J5B5C8_9ASCO
921
0
104018
TrEMBL
Secretory Pathway (Reliability: 1 )
B0E5R6_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
871
0
100268
TrEMBL
Secretory Pathway (Reliability: 3 )
A0A5B6ZEN6_DAVIN
923
0
105143
TrEMBL
Mitochondrion (Reliability: 5 )
A0A0B7HQS7_9FLAO
594
0
67031
TrEMBL
-
A0A3R7Z394_TOXGO
459
0
53658
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A086JU99_TOXGO
993
1
111001
TrEMBL
Mitochondrion (Reliability: 4 )
A0A086KS44_TOXGO
49
1
6111
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A0A1UF38_ENTIV
847
0
96744
TrEMBL
Mitochondrion (Reliability: 5 )
B0E7W7_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
761
0
89625
TrEMBL
other Location (Reliability: 5 )
A0A5B6ZFG2_DAVIN
465
0
53265
TrEMBL
Mitochondrion (Reliability: 5 )
C4LCH3_TOLAT
Tolumonas auensis (strain DSM 9187 / TA4)
788
0
91651
TrEMBL
-
A0A3G2S783_9BASI
996
1
112106
TrEMBL
Secretory Pathway (Reliability: 1 )
M5BSQ7_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
151
0
16417
TrEMBL
other Location (Reliability: 2 )
M5BSR3_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
592
0
67303
TrEMBL
Secretory Pathway (Reliability: 3 )
A0A086PNG9_TOXGO
1005
1
112322
TrEMBL
Mitochondrion (Reliability: 4 )
A0A8B6E2Z9_MYTGA
938
0
107583
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A086JU88_TOXGO
451
0
52702
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A1I9WIF2_ERYPI
239
0
27699
TrEMBL
other Location (Reliability: 4 )
B9WGW8_CANDC
Candida dubliniensis (strain CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841)
867
0
99583
TrEMBL
Secretory Pathway (Reliability: 3 )
A0A369QEA1_9BACT
293
0
32755
TrEMBL
-
A0A061IF92_CRIGR
944
1
106951
TrEMBL
Mitochondrion (Reliability: 5 )
A0A086LU01_TOXGO
1235
2
139296
TrEMBL
Mitochondrion (Reliability: 4 )
A0A5B6ZG17_DAVIN
690
0
79004
TrEMBL
Mitochondrion (Reliability: 4 )
A0A7U3Z4S9_9GAMM
788
0
90167
TrEMBL
-
A0A086KS51_TOXGO
294
0
33413
TrEMBL
other Location (Reliability: 2 )
A0A2G8XTA3_TOXGO
1185
1
133811
TrEMBL
Mitochondrion (Reliability: 4 )
A0A086PNG4_TOXGO
451
0
52702
TrEMBL
Secretory Pathway (Reliability: 2 )
A0A086KS56_TOXGO
273
0
32222
TrEMBL
other Location (Reliability: 2 )
C6DK00_PECCP
Pectobacterium carotovorum subsp. carotovorum (strain PC1)
788
0
91086
TrEMBL
-
E0LY75_9GAMM
787
0
91133
TrEMBL
-
B9STU2_RICCO
923
1
105263
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A2G8XSA6_TOXGO
158
0
18410
TrEMBL
other Location (Reliability: 3 )
A0A2P6RSV1_ROSCH
901
1
102898
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A023BBH9_GRENI
1170
0
130942
TrEMBL
Secretory Pathway (Reliability: 5 )
M5BR65_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
185
0
21172
TrEMBL
other Location (Reliability: 1 )
D7G789_ECTSI
521
0
58620
TrEMBL
other Location (Reliability: 3 )
E0VVD2_PEDHC
721
0
82287
TrEMBL
other Location (Reliability: 1 )
B6AAK9_CRYMR
Cryptosporidium muris (strain RN66)
1234
1
146688
TrEMBL
Secretory Pathway (Reliability: 5 )
M5C2Z0_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
460
0
51793
TrEMBL
Secretory Pathway (Reliability: 2 )
B0EAF9_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
842
0
97395
TrEMBL
Mitochondrion (Reliability: 5 )
B9QIT9_TOXGV
Toxoplasma gondii (strain ATCC 50861 / VEG)
1618
1
183409
TrEMBL
Mitochondrion (Reliability: 4 )
A0A0B7I8P8_9FLAO
1281
1
144632
TrEMBL
-
A0A8B6E2P4_MYTGA
930
0
106639
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A3R7MQZ9_TRYRA
823
1
93310
TrEMBL
Secretory Pathway (Reliability: 1 )
C6C3R4_MUSP7
Musicola paradisiaca (strain Ech703)
788
0
91275
TrEMBL
-
A0A425HRS9_TOXGO
993
1
110886
TrEMBL
Mitochondrion (Reliability: 4 )
F2YBR1_TRIHA
634
0
67638
TrEMBL
Mitochondrion (Reliability: 3 )
B7QCA7_IXOSC
904
0
102694
TrEMBL
other Location (Reliability: 4 )
A0A0U2T0U4_PLAOV
427
0
48179
TrEMBL
other Location (Reliability: 2 )
A0A8J5DZA1_9ASCO
919
0
103665
TrEMBL
Secretory Pathway (Reliability: 1 )
A0A1Z5JSS4_FISSO
743
0
85558
TrEMBL
Mitochondrion (Reliability: 5 )
A0A0B7IG73_9FLAO
500
1
56909
TrEMBL
-
S8GTF8_TOXGM
Toxoplasma gondii (strain ATCC 50611 / Me49)
1616
1
183344
TrEMBL
Mitochondrion (Reliability: 4 )
G7LN15_9GAMM
794
0
91375
TrEMBL
-
A0A7D9H5K0_9GAMM
670
0
75307
TrEMBL
-
Q4J9M3_SULAC
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
627
0
73008
TrEMBL
-
Q96VT3_EMEND
431
0
48072
TrEMBL
Mitochondrion (Reliability: 4 )
GANAB_MOUSE
944
1
106911
Swiss-Prot
Mitochondrion (Reliability: 5 )
GLU2B_SCHPO
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
506
0
57075
Swiss-Prot
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
135000
E16590
by MALDI-TOF mass spectrometry
48000
calculated from amino acid sequence
63800
-
calculated from amino acid sequence
73000
12 * 73000, calculated from sequence
88000
-
gel filtration, SDS-PAGE
67000
-
gel filtration
67000
-
x * 67000, SDS-PAGE
72000
-
SDS-PAGE
72000
12 * 72000, SDS-PAGE
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?
-
x * 67000, SDS-PAGE
?
-
x * 67000, SDS-PAGE
-
dodecamer
12 * 72000, SDS-PAGE
dodecamer
12 * 73000, calculated from sequence
dodecamer
-
12 * 72000, SDS-PAGE
-
dodecamer
-
12 * 73000, calculated from sequence
-
monomer
-
equal molecular weight determined with SDS-PAGE (72000 Da) and gel filtration (67000 Da)
monomer
-
equal molecular weight determined with SDS-PAGE (72000 Da) and gel filtration (67000 Da)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.5 - 6
-
purified enzyme, 45°C, 1 h, stable at
731044
3.5 - 7
-
at 4°C
136906
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
60
E16590
above unstable, stable for 10 min
45
-
stable for 12 h
45
-
purified enzyme, pH 5.5, 1 h, stable
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
alpha-1,3-glucan stabilizes the enzyme
-
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-20°C, 100 mM acetate buffer, pH 5.5
-
4°C, stored for at least 6 months without any loss of activity
below 60°C, 10 min
E16590
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-fold purification and 98% residual enzyme activity after precipitation with propanol, around 2-fold purification and 77% enzyme recovery after salting out with ammonium sulfate at 50% saturation, 10-fold concentrated preparation of the enzyme with a yield of 98% after ultrafiltration, mutanase recovery of 97% after lyophilization and concentration of the culture broth in a vacuum evaporator
-
by chromatographic procedures
E16590
native extracellular enzyme 18.4fold by ultrafiltration, anion exchange and hydrophobic interaction chromatography, and chromatofocusing to homogeneity
-
yield of 56.3% of alkali-soluble and water-insoluble D-glucan
-
-
-
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construction of a cDNA library
expressed in Sulfolobus acidocaldarius. Attempts to obtain soluble enzyme from Escherichia coli strains are unsuccessful
recombinant proteins in E. coli strains NV522 an B221
E16590
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium
the water-insoluble fraction of Laetiporus sulphureus fruiting bodies from 0.15 to 0.2% (w/v) induces mutanase activity in Paenibacillus sp. strain MP-1
the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium
the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium
-
-
the water-insoluble fraction of Laetiporus sulphureus fruiting bodies from 0.15 to 0.2% (w/v) induces mutanase activity in Paenibacillus sp. strain MP-1
-
the water-insoluble fraction of Laetiporus sulphureus fruiting bodies from 0.15 to 0.2% (w/v) induces mutanase activity in Paenibacillus sp. strain MP-1
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
biotechnology
-
transgenic plants might have improved resistance to fungal pathogens
biotechnology
-
transgenic plants might have improved resistance to fungal pathogens
-
medicine
E16590
degradation of Streptococcus mutans biofilms, treatment of dental caries
medicine
-
removal of oral biofilms; possible active ingredients in chewing gum, mouthwash, dental gel, toothpaste
medicine
-
degradation of Streptococcus mutans biofilms, treatment of dental caries
-
medicine
-
removal of oral biofilms; possible active ingredients in chewing gum, mouthwash, dental gel, toothpaste
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tsunoda, A.; Sakano, Y.; Kobayashi, T.
Purification and properties of an exo-alpha-1,3-glucanase from Cladosporium resinae
Agric. Biol. Chem.
42
1045-1053
1978
Amorphotheca resinae
-
brenda
Tsunoda, A.; Nagaki, T.; Sakano, Y.; Kobayashi, T.
Purification and properties of an exo-alpha-1,3-glucanase from Trichoderma viride
Agric. Biol. Chem.
41
939-943
1977
Trichoderma sp.
-
brenda
Zonneveld, B.J.M.
A new type of enzyme, and exo-splitting alpha-1,3 glucanase from non-induced cultures of Aspergillus nidulans
Biochim. Biophys. Acta
258
541-547
1972
Aspergillus nidulans
brenda
Molyneux, R.J.; Roitman, J.N.; Dunnheim, G.; Szumilo, T.; Elbein, A.D.
6-Epicastanospermin, a novel indolizidine alkaloid that inhibits alpha-glucosidase
Arch. Biochem. Biophys.
251
450-457
1986
Vigna radiata
brenda
Wei, H.; Scherer, M.; Singh, A.; Liese, R.; Fischer, R.
Aspergillus nidulans alpha-1,3 glucanase (mutanase), mutA, is expressed during sexual development and mobilizes mutan
Fungal Genet. Biol.
34
217-227
2001
Aspergillus nidulans (Q96VT3)
brenda
Ait-Lahsen, H.; Soler, A.; Rey, M.; de La Cruz, J.; Monte, E.; Llobell, A.
An antifungal exo-alpha-1,3-glucanase (AGN13.1) from the biocontrol fungus Trichoderma harzianum
Appl. Environ. Microbiol.
67
5833-5839
2001
Trichoderma harzianum, Trichoderma harzianum CECT2413
brenda
Shimotsuura, I.; Kigawa, H.; Ohdera, M.; Kuramitsu, H.K.; Nakashima, S.
Biochemical and Molecular Characterization of a Novel Type of Mutanase from Paenibacillus sp. Strain RM: Identificatiion of its Mutan-Binding Domain, Essential for Degradation of Streptococcus mutans Biofilms
Appl. Environ. Microbiol.
74
2759-2765
2008
Paenibacillus sp. (E16590), Paenibacillus sp., Paenibacillus sp. RM1 (E16590)
brenda
Wiater, A.; Szczodrak, J.; Pleszczynskaa, M.
Mutanase Induction in Trichoderma harzianum by Cell Wall of Laetiporus sulphureus and its Application for Mutan Removal from Oral Biofilms
J. Microbiol. Biotechnol.
18
1335-1341
2008
Trichoderma harzianum, Trichoderma harzianum F-340
brenda
Pleszczynska, M.; Wiater, A.; Szczodrak, J.
Methods for Obtaining Active Mutanase Preparations from Paenibacillus curdlanolyticus
Prep. Biochem. Biotechnol.
38
389-396
2008
Paenibacillus curdlanolyticus, Paenibacillus curdlanolyticus MP-1
brenda
Pleszczy?ska, M.; Wiater, A.; Szczodrak, J.
Mutanase from Paenibacillus sp. MP-1 produced inductively by fungal alpha-1,3-glucan and its potential for the degradation of mutan and Streptococcus mutans biofilm
Biotechnol. Lett.
32
1699-1704
2010
Paenibacillus sp., Paenibacillus sp. MP-1
brenda
Choi, K.H.; Hwang, S.; Cha, J.
Identification and characterization of MalA in the maltose/maltodextrin operon of Sulfolobus acidocaldarius DSM639
J. Bacteriol.
195
1789-1799
2013
Sulfolobus acidocaldarius (Q4J9M3), Sulfolobus acidocaldarius DSM 639 (Q4J9M3)
brenda
Wiater, A.; Pleszczynska, M.; Rogalski, J.; Szajnecka, L.; Szczodrak, J.
Purification and properties of an alpha-(1->3)-glucanase (EC 3.2.1.84) from Trichoderma harzianum and its use for reduction of artificial dental plaque accumulation
Acta Biochim. Pol.
60
123-128
2013
Trichoderma harzianum, Trichoderma harzianum CCM F-340
brenda
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