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1-kestose + H2O
D-fructose + sucrose
3 levan + 7 H2O
10 levanbiose
Pseudomonas sp. No. 43
-
-
-
?
cellulose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
D(+)-raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
D(+)-raffinose + H2O
D-fructose + ?
-
-
-
?
D-(+)-xylose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
dextran + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
Erwinia herbicola levan + H2O
D-fructose + fructooligosacchrides
-
-
-
-
?
fructose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
galactose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
glucose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
high molecular weight levan + H2O
D-fructose + ?
high molecular weight levan + H2O
D-fructose + fructooligosaccharides DP2-10
-
-
-
-
?
inulin + H2O
D-fructose + ?
lactose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
levan + H2O
D-fructose + ?
levan + H2O
D-fructose + fructooligosaccharides
-
-
-
?
levan + H2O
fructose + beta-1,2-oligofructans
levan + H2O
levanbiose + D-fructose
-
-
final products, thinlayer chromatography analysis
-
?
levan + H2O
levanheptaose
-
-
-
?
levan + H2O
O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ?
levan + H2O
oligofructosides
levan-type fructans (beta-2,6)
D-fructose
-
-
-
-
?
levanotetraose + H2O
?
-
-
-
-
?
levans + H2O
oligofructosides
low molecular weight levan + H2O
D-fructose + ?
low molecular weight levan + H2O
D-fructose + fructooligosaccharides DP2-10
-
-
-
-
?
maltose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
raffinose + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
soluble starch + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
sucrose + H2O
D-fructose + D-glucose
Timothy grass fructan + H2O
?
xylan + H2O
?
Pseudomonas strain K-52
-
-
-
-
?
levan + H2O
D-fructose + ?
1-kestose + H2O
D-fructose + sucrose
-
-
-
?
1-kestose + H2O
D-fructose + sucrose
-
-
-
?
D(+)-raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
-
-
-
-
?
D(+)-raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
-
-
-
-
?
high molecular weight levan + H2O
D-fructose + ?
-
minor activity
-
-
?
high molecular weight levan + H2O
D-fructose + ?
-
minor activity
-
-
?
inulin + H2O
?
-
-
-
-
?
inulin + H2O
D-fructose + ?
-
without formation of intermediate oligofructans
-
-
?
inulin + H2O
D-fructose + ?
without formation of intermediate oligofructans
-
-
?
inulin + H2O
D-fructose + ?
-
without formation of intermediate oligofructans
-
-
?
inulin + H2O
D-fructose + ?
without formation of intermediate oligofructans
-
-
?
levan + H2O
?
-
commercial Erwinia herbicola levan and levan from Leuconostoc mesenteroides DSM 20343-fermented fava bean doughs, combined activity of exoinulinase/levanase
-
-
?
levan + H2O
?
-
substrate from Erwinia herbicola
-
-
?
levan + H2O
?
-
substrate from Erwinia herbicola
-
-
?
levan + H2O
D-fructose + ?
4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
-
?
levan + H2O
D-fructose + ?
-
without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
-
?
levan + H2O
D-fructose + ?
-
without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
-
?
levan + H2O
D-fructose + ?
4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
-
?
levan + H2O
D-fructose + ?
-
-
-
?
levan + H2O
fructose + ?
-
-
-
?
levan + H2O
fructose + ?
-
-
-
?
levan + H2O
fructose + beta-1,2-oligofructans
-
-
-
?
levan + H2O
fructose + beta-1,2-oligofructans
-
-
-
?
levan + H2O
O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ?
almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase
major product
-
?
levan + H2O
O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ?
almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase
major product
-
?
levan + H2O
oligofructosides
-
-
-
?
levan + H2O
oligofructosides
-
-
-
?
levan + H2O
oligofructosides
-
-
-
?
levan + H2O
oligofructosides
-
-
-
?
levan + H2O
oligofructosides
-
-
-
?
levan + H2O
oligofructosides
-
-
-
?
levan + H2O
oligofructosides
-
-
-
?
levan + H2O
oligofructosides
yeasts
-
-
-
?
levan + H2O
oligofructosides
yeasts
-
-
-
?
levans + H2O
oligofructosides
-
-
-
?
levans + H2O
oligofructosides
-
-
-
?
levans + H2O
oligofructosides
-
-
-
?
levans + H2O
oligofructosides
-
-
-
?
levans + H2O
oligofructosides
-
-
-
?
levans + H2O
oligofructosides
-
-
-
?
low molecular weight levan + H2O
D-fructose + ?
-
minor activity
-
-
?
low molecular weight levan + H2O
D-fructose + ?
-
minor activity
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
-
-
-
?
Timothy grass fructan + H2O
?
-
-
-
-
?
Timothy grass fructan + H2O
?
-
-
-
-
?
levan + H2O
D-fructose + ?
additional information
-
-
-
-
?
additional information
?
-
-
in order to develop a specific analysis for levan in food matrices, a Paenibacillus amylolyticus endolevanase (EC 3.2.1.65) is combined with exoinulinase (EC 3.2.1.80) for levan hydrolysis achieving about 80% hydrolysis yield, method evaluation, and modelling. The levanase preparation exhibits an activity of 1116 U/ml towards levan and no activity towards inulin as determined by the reducing value method
-
-
?
additional information
?
-
a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C
-
-
?
additional information
?
-
-
a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C
-
-
?
additional information
?
-
-
the levansucrase-endolevanase fusion enzyme LevB1SacB or both enzymes (levanase LevB1 and levansucrase SacB) at equimolar concentrations in simultaneous reactions result in an optimal, wide and diverse L-FOS profile, including 6-kestose, levanbiose and blastose among other L-FOS and 1-kestose, which accumulates as by-product of SacB levan synthesis
-
-
?
additional information
?
-
a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C
-
-
?
additional information
?
-
-
enzyme reactions with levan in the presence of D-glucose and D-fructose results in the production of a wide variety of oligosaccharides such as blastose, levanobiose, inulobiose, 1-kestose and 6-kestose
-
-
?
additional information
?
-
-
enzyme reactions with levan in the presence of D-glucose and D-fructose results in the production of a wide variety of oligosaccharides such as blastose, levanobiose, inulobiose, 1-kestose and 6-kestose
-
-
?
additional information
?
-
-
the exo-acting enzyme is capable of hydrolyzing not only beta-2,6-fructosyl linkages, but also beta-2,1-fructosyl linkages
-
-
?
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Molecular characterization of Bacillus subtilis levanase and a C-terminal deleted derivative
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247-258
1988
Bacillus subtilis, Escherichia coli
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1987
Bacillus subtilis, Escherichia coli
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Purification and characterization of levanase from Actinomyces viscosus ATCC19246
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55
3001-3005
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Actinomyces viscosus, Bacillus subtilis
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Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase
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208
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Bacillus subtilis, Escherichia coli
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Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis
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3
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Actinomyces viscosus, Bacillus subtilis, Escherichia coli
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Bacteria and yeasts as possible candidates for the production of inulinases and levanases
Antonie van Leeuwenhoek
51
333-351
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Enterobacter cloacae, Trinickia caryophylli, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, yeasts
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Isolation and properties of levanase from Streptococcus salivarius KTA-19
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42
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Bacillus subtilis, Streptococcus salivarius
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Fructan hydrolases
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Arthrobacter sp.
-
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Use of homologous expression-secretion signals and vector-free stable chromosomal integration in engineering of Lactobacillus plantarum for alpha-amylase and levanase expression
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60
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Bacillus subtilis, Lactiplantibacillus plantarum
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Expression of Bacillus subtilis levanase gene in Lactobacillus plantarum and Lactobacillus casei
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43
297-303
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Bacillus subtilis, Lactiplantibacillus plantarum, Lacticaseibacillus casei, yeasts
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Purification and characterization of the Bacillus subtilis levanase produced in Escherichia coli
Appl. Environ. Microbiol.
61
1953-1958
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Bacillus subtilis, Escherichia coli
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Purification and characterization of a novel levanoctaoase-producing levanase from Pseudomonas strain K-52
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27
159-166
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Pseudomonas strain K-52
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46
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Rhodotorula sp.
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Purification and charakterization of a levanase from Streptomyces sp. 366L
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61
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Streptomyces sp. 366L
-
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Purification and characterization of a levanbiose-producing levanase from Pseudomonas sp. No. 43
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29
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Pseudomonas sp. No. 43
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Molecular cloning and expression in Escherichia coli of an exo-levanase gene from the endophytic bacterium Gluconacetobacter diazotrophicus SRT4
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45
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Gluconacetobacter diazotrophicus (Q43998), Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4 (Q43998), Gluconacetobacter diazotrophicus SRT4
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Functional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris
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-
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Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.)
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Beta vulgaris
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The enzymatic activity from the sediment of the Gilau dam reservoir - Cluj county
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69
261-272
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More
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Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus
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75
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Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4
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Fructanolytic and saccharolytic enzymes of Treponema zioleckii strain kT
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16
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Treponema zioleckii, Treponema zioleckii kT
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Efficient simultaneous saccharification and fermentation of inulin to 2,3-butanediol by thermophilic Bacillus licheniformis ATCC 14580
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Microbial production of levanase for specific hydrolysis of levan
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uncultured bacterium
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Levan-type FOS production using a Bacillus licheniformis endolevanase
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Levan-type fructooligosaccharides synthesis by a levansucrase-endolevanase fusion enzyme (LevB1SacB)
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208
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Intrinsic levanase activity of Bacillus subtilis 168 levansucrase (SacB)
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