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1-galactosyl-mannotriose + H2O
?
-
-
-
-
?
2,4-dinitrophenyl-beta-D-mannopyranoside + H2O
2,4-dinitrophenol + alpha-D-mannose
-
-
-
-
?
2-acetamido-2-deoxymannopyranose + H2O
?
-
-
-
-
?
2-amino-2-deoxymannopyranose + H2O
?
-
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
4-nitrophenol beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
-
129% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + beta-D-glucose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannose
-
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannopyranose
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
4-nitrophenyl beta-D-mannopyranoside + methyl alpha-D-mannopyranoside
4-nitrophenyl alpha-D-mannopyranoside + methyl beta-D-mannopyranoside
-
the enzyme shows low transglycosylation activity at 50°C and pH 5.0
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
3.1% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-L-arabinopyranoside + H2O
4-nitrophenol + beta-L-arabinopyranose
-
16% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + 1-propanol
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-fructose
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-galactose
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-xylose
?
-
transglycosylation reaction, 18.4% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + ethanol
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
4-O-beta-D-mannopyranosyl-D-mannopyranose + L-arabinose
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + methanol
?
-
transglycosylation reaction, 42.6% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + N-acetyl-D-glucosamine
?
-
transglycosylation reaction, 1.4% transfer ratio
-
-
?
alpha-D-mannopyranose 1-phosphate + H2O
alpha-D-mannose + phosphate
-
-
-
-
?
alpha-Man-(1-4)-beta-Man-(1-4)-Man + H2O
?
-
-
-
-
?
Asn-(GlcNAc)2(Man) + H2O
?
-
-
-
-
?
beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-Asn-Lys + H2O
?
-
-
-
-
?
beta-D-Man-(1-4)-beta-D-Man-(1-4)-D-Man + H2O
?
-
-
-
-
?
beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc(1->6)]-beta-D-GlcNAc-Asn-peptide + H2O
beta-D-GlcNAc-(1->4)-[alpha-L-Fuc(1->6)]-beta-D-GlcNAc-Asn-peptide + D-mannopyranose
-
-
-
-
?
beta-D-mannohexaitol + H2O
?
beta-D-mannopentaitol + H2O
?
beta-D-mannosyl-1,4-beta-D-glucoside + H2O
D-mannose + beta-D-glucose
-
-
-
-
?
beta-D-mannotetraitol + H2O
?
beta-D-mannotriitol + H2O
?
beta-mannotetraitol + H2O
?
beta1-4-mannobiose + H2O
?
cellobiose + H2O
?
-
33% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellohexaose + H2O
?
-
303% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellopentaose + H2O
?
-
225% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellotetraose + H2O
?
-
52% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellotriose + H2O
?
-
19% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
D-mannofuranurono-6,3-lactone + H2O
?
-
-
-
-
?
D-mannoheptulose + H2O
?
-
-
-
-
?
D-mannono-1,4-lactone + H2O
?
-
-
-
-
?
L-mannono-1,4-lactone + H2O
?
-
-
-
-
?
laminaribiose + H2O
?
-
13% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
laminaritriose + H2O
?
-
2.8% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
Man-(beta1-4)GlcNAc + H2O
mannose + GlcNAc
Man-(beta1-4)ManNAc + H2O
mannose + ManNAc
-
-
-
?
Man-GlcNAc-GlcNAc + H2O
mannose + GlcNAc-GlcNAc
-
-
-
?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O
mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn
Manbeta1-2Man + H2O
D-mannose
Manbeta1-4GlcNAcbeta1-4Manbeta1-4GlcNAcbeta1-4GlcNAc + H2O
mannose + GlcNAcbeta1-4Manbeta1-4GlcNAcbeta1-4GlcNAc
-
-
-
?
Manbeta1-4Man + H2O
D-mannose
-
-
-
-
?
mannobiose + glucose + D-glucose
?
-
transglycosylation reaction, 40.1% transfer ratio
-
-
?
mannobiose + H2O
2 D-mannopyranose
mannopyranosylamine + H2O
?
-
-
-
-
?
mannotetraose + H2O
4 D-mannopyranose
mannotriitol + H2O
?
-
-
-
-
?
mannotriose + H2O
3 D-mannopyranose
methyl alpha-D-mannopyranoside + H2O
?
-
-
-
-
?
naphthyl-beta-D-mannopyranoside + H2O
2-naphthol + beta-D-mannose
-
-
-
-
?
o-nitrophenyl-beta-D-mannopyranoside + H2O
o-nitrophenol + alpha-D-mannose
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannopyranose
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
4.6% activity compared to p-nitrophenyl-beta-D-mannoside
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
34.5% activity compared to p-nitrophenyl-beta-D-mannoside
-
-
?
p-nitrophenyl-beta-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannose
-
self-transfer of the mannosyl group is observed, and a 10-15% yield of a beta1,4-disaccharide is obtained
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
p-nitrophenyl-beta-galactoside + H2O
p-nitrophenol + beta-D-galactopyranose
-
low activity
-
-
?
p-nitrophenyl-beta-mannoside + H2O
p-nitrophenol + beta-D-mannopyranose
-
-
-
-
?
phenyl beta-D-mannoside + H2O
?
sophorose + H2O
?
-
15% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
additional information
?
-
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
4-methylumbelliferyl beta-D-mannopyranoside
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
-
-
-
?
4-nitrophenol beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-nitrophenol beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
17% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-galactopyranoside is 20% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-galactopyranoside is 20% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-glucopyranoside is 26% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-mannopyranoside. The catalytic efficiency (kcat/Km) of the mutant enzymes Q77R, D206N and Q77R/D206N for 4-nitrophenyl beta-D-glucopyranoside is higher then that for 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-glucopyranoside is 26% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-mannopyranoside. The catalytic efficiency (kcat/Km) of the mutant enzymes Q77R, D206N and Q77R/D206N for 4-nitrophenyl beta-D-glucopyranoside is higher then that for 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
238% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannopyranose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannopyranose
-
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + 1-propanol
?
-
transglycosylation reaction, 50% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + 1-propanol
?
-
transglycosylation reaction, 50% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-fructose
?
-
transglycosylation reaction, 72.3% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-fructose
?
-
transglycosylation reaction, 72.3% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-galactose
?
-
transglycosylation reaction, 14.2% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-galactose
?
-
transglycosylation reaction, 14.2% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + ethanol
?
-
transglycosylation reaction, 41.1% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + ethanol
?
-
transglycosylation reaction, 41.1% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
-
-
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
-
-
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
-
-
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + L-arabinose
?
-
transglycosylation reaction, 6.4% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + L-arabinose
?
-
transglycosylation reaction, 6.4% transfer ratio
-
-
?
beta-D-mannohexaitol + H2O
?
-
-
-
-
?
beta-D-mannohexaitol + H2O
?
-
-
-
-
?
beta-D-mannopentaitol + H2O
?
-
-
-
-
?
beta-D-mannopentaitol + H2O
?
-
-
-
-
?
beta-D-mannotetraitol + H2O
?
-
-
-
-
?
beta-D-mannotetraitol + H2O
?
-
-
-
-
?
beta-D-mannotriitol + H2O
?
-
-
-
-
?
beta-D-mannotriitol + H2O
?
-
-
-
-
?
beta-D-mannotriitol + H2O
?
-
-
-
-
?
beta-mannotetraitol + H2O
?
-
-
-
-
?
beta-mannotetraitol + H2O
?
-
-
-
-
?
beta1-4-mannobiose + H2O
?
Polyporus sulfureus
-
-
-
-
?
beta1-4-mannobiose + H2O
?
-
-
-
-
?
beta1-4-mannobiose + H2O
?
-
-
-
-
?
locust bean gum + H2O
?
-
-
-
-
?
locust bean gum + H2O
?
-
-
-
-
?
locust bean gum + H2O
?
-
-
-
-
?
Man-(beta1-4)GlcNAc + H2O
mannose + GlcNAc
-
-
-
?
Man-(beta1-4)GlcNAc + H2O
mannose + GlcNAc
Polyporus sulfureus
-
-
-
?
Man-(beta1-4)GlcNAc + H2O
mannose + GlcNAc
-
-
-
-
?
Man-(beta1-4)GlcNAc + H2O
mannose + GlcNAc
-
-
-
-
?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O
mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn
-
-
-
?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O
mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn
Polyporus sulfureus
-
-
-
-
?
Manbeta1-2Man + H2O
D-mannose
-
-
-
-
?
Manbeta1-2Man + H2O
D-mannose
-
-
-
-
?
mannan + H2O
mannose + ?
-
galactomannan
-
?
mannan + H2O
mannose + ?
-
glucomannans and galactomannans are completely hydrolyzed
-
-
?
mannobiose + H2O
2 D-mannopyranose
-
-
-
-
?
mannobiose + H2O
2 D-mannopyranose
activity is 15% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannobiose + H2O
2 D-mannopyranose
activity is 15% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannopentaose + H2O
?
-
-
-
-
?
mannopentaose + H2O
?
-
-
-
-
?
mannotetraose + H2O
4 D-mannopyranose
activity is 32% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotetraose + H2O
4 D-mannopyranose
activity is 32% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotetraose + H2O
?
-
-
-
-
?
mannotetraose + H2O
?
-
-
-
-
?
mannotetraose + H2O
?
-
-
-
-
?
mannotriose + H2O
3 D-mannopyranose
activity is 47% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotriose + H2O
3 D-mannopyranose
activity is 47% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotriose + H2O
?
-
-
-
-
?
mannotriose + H2O
?
-
-
-
-
?
mannotriose + H2O
?
-
-
-
-
?
mannotriose + H2O
?
Polyporus sulfureus
-
-
-
-
?
mannotriose + H2O
?
-
beta1-4-D-mannotriose
-
-
?
mannotriose + H2O
?
-
beta1-4-D-mannotriose
-
-
?
o-nitrophenyl-beta-D-mannopyranoside + H2O
o-nitrophenol + alpha-D-mannose
-
-
-
?
o-nitrophenyl-beta-D-mannopyranoside + H2O
o-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannopyranose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannopyranose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
no activity with p-nitrophenyl-alpha-D-mannoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
hydrolyses 4-nitrophenyl-beta-D-mannoside three times faster than p-nitrophenyl-beta-D-glucoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
Polyporus sulfureus
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
phenyl beta-D-mannoside + H2O
?
-
-
-
-
?
phenyl beta-D-mannoside + H2O
?
-
-
-
-
?
additional information
?
-
-
hydrolyses D-galactose substituted manno-oligosaccharides up to, but not beyond, a side group
-
-
?
additional information
?
-
-
locust bean gum and guar flour are the best substrates for enzyme formation
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
-
the Michaelis complex of the beta-mannosidase Man2A shows distortion to a 1S5 conformation supporting catalysis through a boat conformation
-
-
?
additional information
?
-
-
deficiency of lysosomal beta-mannosidase activity results in a severe neurodegenerative disease in goats and cattle and a relatively milder phenotype in humans
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
goat liver contains both a lysosomal beta-mannosidase, acidic enzyme form, deficient in beta-mannosidosis, and a neutral nonlysosomal
-
-
?
additional information
?
-
-
Glu519 is the catalytic nucleophile in beta-mannosidase 2A
-
-
?
additional information
?
-
-
no hydrolysis of p-nitrophenyl derivatives of alpha-mannose, beta-N-acetylglucosamine, beta-glucose, beta-xylose, beta-cellobiose or beta-gentiobiose
-
-
?
additional information
?
-
-
CmMan5A releases beta-1,4-linked mannose substrates exclusively from the nonreducing end
-
-
?
additional information
?
-
-
the enzyme plays a key role in the hydrolysis of galactomannan in germinating guar seed
-
-
?
additional information
?
-
no substrate: 4-nitrophenyl-alpha-D-mannose
-
-
?
additional information
?
-
-
no substrate: 4-nitrophenyl-alpha-D-mannose
-
-
?
additional information
?
-
-
4-nitrophenyl-N-acetyl-beta-D-glucosaminide, 4-nitrophenyl-beta-D-xyloside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-gentiobioside, and 4-nitrophenyl-cellobioside are no substrates
-
-
?
additional information
?
-
-
comparison with the activities of rice family GH1 glycoside hydrolases
-
-
?
additional information
?
-
in glycoside recognition and substrate specificity of HvBII, a combination of the following determinants is likely to play key roles: the inherent conformational and spatial flexibilities of gluco- and manno-configured substrates in the enzymes' active sites, the subtle differences in the spatial disposition of active site residues and their capacities to form interactions with specific groups of substrates, and the small variations in the charge distributions and shapes of the catalytic sites
-
-
?
additional information
?
-
-
in glycoside recognition and substrate specificity of HvBII, a combination of the following determinants is likely to play key roles: the inherent conformational and spatial flexibilities of gluco- and manno-configured substrates in the enzymes' active sites, the subtle differences in the spatial disposition of active site residues and their capacities to form interactions with specific groups of substrates, and the small variations in the charge distributions and shapes of the catalytic sites
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
Polyporus sulfureus
-
the enzyme liberates mannose from core glycopeptide isolated from Taka-amylase A and alpha1-acid glycoprotein
-
-
?
additional information
?
-
-
extensively hydrolyzes the carbohydrate portion of glycopeptides obtained from ovalbumin
-
-
?
additional information
?
-
-
beta-mannosidase and endo-beta-mannanase are involved in the mobilization of the mannan-containing cell walls of the tomato seed endosperm. A lack of coordination of the activities of these two enzymes within individuals of a population
-
-
?
additional information
?
-
-
coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan
-
-
?
additional information
?
-
-
coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan
-
-
?
additional information
?
-
-
calculation of conformational free-energy surface of a beta-D-mannopyranose molecule associated with the ideal Stoddart conformational diagram. 1S5 is among the most stable conformers and simultaneously is the most preactivated conformation in terms of elongation/shortening of the C1-O1/C1-O5 bonds, C1-O1 orientation, and charge development at the anomeric carbon. Analysis of the computed free-energy surface gives support to the proposed 1S5-B2,5-OS2 catalytic itinerary. The degree of preactivation of the substrate in glycoside hydrolases is related to the properties of an isolated sugar ring. Introduction of a simple preactivation index integrating several structural, electronic, and energetic properties that can be used to predict the conformation of the substrate in the Michaelis complex of any glycoside hydrolase
-
-
?
additional information
?
-
the enzyme also exhibited transglycosidase activity when the reactionwas carried out with 10% (w/v) of mannobiose in the presence of alcohols or galactose
-
-
?
additional information
?
-
the enzyme also exhibited transglycosidase activity when the reactionwas carried out with 10% (w/v) of mannobiose in the presence of alcohols or galactose
-
-
?
additional information
?
-
-
in addition to hydrolysis, enzyme catalyzes transglycosylation with substrate 4-nitrophenyl-beta-D-mannopyranoside to yield 4-nitrophenyl-mannobiosides and 4-nitrophenyl mannotriosides containing beta-(1,4) and beta-(1,3) linkages. The rate of donor substrate hydrolysis increases in presence of acetonitrile and dimethylformamide
-
-
?
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(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
-
(5S,6R,8R)-5-(hydroxymethyl)-2-(2-phenylethyl)-5,6,7,8-tetrahydroimidazo[1,2-a]pyridine-6,7,8-triol
persuasive transition state mimic
(5S,6R,8R)-5-(hydroxymethyl)-2-(phenoxymethyl)-5,6,7,8-tetrahydroimidazo[1,2-a]pyridine-6,7,8-triol
persuasive transition state mimic
(5S,6R,8R)-5-(hydroxymethyl)-2-[(phenylamino)methyl]-5,6,7,8-tetrahydroimidazo[1,2-a]pyridine-6,7,8-triol
persuasive transition state mimic
2-amino-2-deoxy-D-mannose
2-deoxy-2-fluoro-beta-D-mannosyl fluoride
-
time-dependent inactivation through accumulation of a covalent 2-deoxy-2-fluoro-alpha-D-mannosyl-beta-mannosidase 2A enzyme intermediate
2-deoxy-2-fluoro-beta-glycosyl fluorides
-
in vivo inhibition
-
4-nitrophenyl beta-D-thioglucoside
competitive, adopts 4NP-S-Glc 3S5 or 1S3 conformations upon binding according to STD NMR and trNOESY experiments. QM modeling and docking, based on GLIDE scores, predicts that 4-nitrophenyl beta-D-thioglucoside preferentially binds in 1S3 geometries
4-nitrophenyl beta-D-thiomannoside
competitive, adopts 4C1 geometry upon binding according to STD NMR and trNOESY experiments. QM modeling and docking, based on GLIDE scores, predicts that 4-nitrophenyl beta-D-thiomannoside preferentially binds in 1S3 geometries
beta-1,4-mannooligosaccharides
-
inhibit binding of the enzyme to galactomannans
-
Ca2+
-
5 mM Ca(NO3)2, 45% inhibition
D-mannono-1,4-lactone
-
-
dimethyl sulfoxide
5 mM, no residual activity
dimethylformamide
5 mM, no residual activity
ethanol
5 mM, no residual activity
Fe3+
Polyporus sulfureus
-
-
guanidine hydrochloride
-
0.6 M, 30% inhibition
iodoacetate
5 mM, no residual activity
mannose
-
relative inhibition of 20%, three concentrations of 10, 50, and 100 mM tested
Mg2+
1 mM, 4% loss of activity
Mn2+
1 mM, 4% loss of activity
N,N-(2-aminoethyl)-D-glucoamidine
-
-
N,N-(2-aminoethyl)-D-mannoamidine
-
-
N,N-(3-aminopropyl)-D-glucoamidine
-
-
N,N-(3-aminopropyl)-D-mannoamidine
-
-
N,N-(3-hydroxypropyl)-D-glucoamidine
-
-
N,N-(3-hydroxypropyl)-D-mannoamidine
-
-
N,N-(4-aminobutyl)-D-glucoamidine
-
-
N,N-(4-aminobutyl)-D-mannoamidine
-
-
N,N-propyl-D-mannoamidine
-
-
n-Dodecylbenzene sulfonate
-
-
N-propyl-D-glucoamidine
-
-
N-[(2Z,3R,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]ethane-1,2-diaminium
-
N-[(2Z,3R,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]propan-1-aminium
-
p-nitrophenyl-alpha-D-mannopyranoside
p-nitrophenyl-beta-mannoside
-
above 0.4 mM
Sodium dodecyl sulfate
5 mM, no residual activity
2-amino-2-deoxy-D-mannose
-
competitive
2-amino-2-deoxy-D-mannose
-
-
Ag+
-
-
Ag+
1.5 mM, no residual activity
Ag+
Polyporus sulfureus
-
2 mM, complete inhibition
Ag+
1 mM, 98% loss of activity
Cd2+
-
-
Co2+
1.5 mM, 80% residual activity
Co2+
1 mM, 12% loss of activity
Cu2+
-
-
Cu2+
1.5 mM, no residual activity
Cu2+
Polyporus sulfureus
-
-
Cu2+
1 mM, 72% loss of activity
Cu2+
-
0.2 mM, 76% inhibition
D-mannose
-
with p-nitrophenyl-beta-D-mannopyranoside as substrate
D-mannose
Polyporus sulfureus
-
-
D-mannose
-
hydrolysis of p-nitrophenyl beta-D-mannoside, competitive
Hg2+
-
-
Hg2+
-
0.05 mM, 50% inhibition
Hg2+
Polyporus sulfureus
-
-
Hg2+
1 mM, 98% loss of activity
p-nitrophenyl-alpha-D-mannopyranoside
-
hydrolysis of p-nitrophenyl-beta-D-mannoside
p-nitrophenyl-alpha-D-mannopyranoside
-
activates at low concentration, competitively inhibits at higher concentrations, 2-3 mM
Pb2+
1.5 mM, 78% residual activity
PCMB
-
-
PMSF
-
-
PMSF
Polyporus sulfureus
-
-
SDS
-
-
Zn2+
-
-
Zn2+
1.5 mM, no residual activity
Zn2+
Polyporus sulfureus
-
-
Zn2+
-
10 mM ZnSO4, 30% inhibition
Zn2+
-
0.1 mM, 35% inhibition
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0.0028 - 0.089
2,4-dinitrophenyl-beta-D-mannopyranoside
0.13 - 0.91
4-methylumbelliferyl beta-D-mannopyranoside
0.94 - 1.29
4-nitrophenyl beta-D-fucopyranoside
7.3 - 36.1
4-nitrophenyl beta-D-galactopyranoside
0.3 - 30.4
4-nitrophenyl beta-D-glucopyranoside
0.4 - 3.2
4-nitrophenyl beta-D-mannopyranoside
0.5
4-nitrophenyl-beta-D-glucopyranose
pH 4.0, 30°C
0.25 - 1.6
4-nitrophenyl-beta-D-mannopyranose
2.5 - 3
4-nitrophenyl-beta-D-mannopyranoside
pH 5.0, 37°C
0.000083
4-nitrophenyl-beta-mannopyranose
-
pH 7.0, 37°C, mutant enzyme E215A
16.9
Asn-(GlcNAc)2(Man)
-
-
2.8 - 12.5
Beta-D-mannohexaitol
2.8 - 12.5
beta-D-mannopentaitol
6.1
beta-D-mannosyl-1,4-beta-D-glucoside
-
pH 7.0, 37°C, wild-type enzyme
12 - 12.5
beta-D-mannotetraitol
12.5 - 80
beta-D-mannotriitol
0.9
mannobiose
-
pH 7.0, 37°C, wild-type enzyme
0.1
Mannotriose
-
pH 7.0, 37°C, wild-type enzyme
3.22
Naphthyl-beta-D-mannopyranoside
-
-
2.33 - 3
o-nitrophenyl-beta-D-mannopyranoside
0.55 - 9
p-nitrophenyl beta-D-mannopyranoside
0.017 - 11
p-nitrophenyl-beta-D-mannopyranoside
0.12 - 5
p-nitrophenyl-beta-D-mannoside
6.5 - 7.1
phenyl beta-D-mannoside
additional information
additional information
-
as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the KM-value of 0.3 mM is only an estimate
-
0.0028
2,4-dinitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.015
2,4-dinitrophenyl-beta-D-mannopyranoside
-
mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.088
2,4-dinitrophenyl-beta-D-mannopyranoside
-
wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.089
2,4-dinitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.13
4-methylumbelliferyl beta-D-mannopyranoside
-
beta-mannosidase B
0.35
4-methylumbelliferyl beta-D-mannopyranoside
-
beta-mannosidase A
0.5
4-methylumbelliferyl beta-D-mannopyranoside
-
-
0.56
4-methylumbelliferyl beta-D-mannopyranoside
-
-
0.8
4-methylumbelliferyl beta-D-mannopyranoside
-
-
0.91
4-methylumbelliferyl beta-D-mannopyranoside
-
-
0.94
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, wild-type
1.29
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
7.3
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, wild-type enzyme
7.8
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme D206N
16.8
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
36.1
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
0.3
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme D206N
4.6
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, wild-type
9.23
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
13.5
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, wild-type enzyme
16.2
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
30.4
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
0.4
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
0.44
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, wild-type enzyme
0.49
4-nitrophenyl beta-D-mannopyranoside
pH 7.0, 70°C
0.68
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
1.08
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, wild-type
1.1
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
3.2
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme D206N
0.25
4-nitrophenyl-beta-D-mannopyranose
pH 4.0, 30°C
1.6
4-nitrophenyl-beta-D-mannopyranose
-
pH 7.0, 37°C, wild-type enzyme
2.8
Beta-D-mannohexaitol
-
-
12.5
Beta-D-mannohexaitol
-
-
2.8
beta-D-mannopentaitol
-
-
12.5
beta-D-mannopentaitol
-
-
12
beta-D-mannotetraitol
-
-
12.5
beta-D-mannotetraitol
-
-
12.5
beta-D-mannotriitol
-
-
80
beta-D-mannotriitol
-
-
1.2
mannotriitol
-
enzyme from endosperm
10
mannotriitol
-
enzyme from cotyledons
2.33
o-nitrophenyl-beta-D-mannopyranoside
-
-
3
o-nitrophenyl-beta-D-mannopyranoside
-
-
0.55
p-nitrophenyl beta-D-mannopyranoside
-
-
3.1
p-nitrophenyl beta-D-mannopyranoside
-
-
9
p-nitrophenyl beta-D-mannopyranoside
-
-
0.017
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W395A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.079
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W198A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.18
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.19
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.19
p-nitrophenyl-beta-D-mannopyranoside
-
wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.3
p-nitrophenyl-beta-D-mannopyranoside
-
-
0.3
p-nitrophenyl-beta-D-mannopyranoside
-
-
0.31
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme Q646A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.31
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme Y537A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.43
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.46
p-nitrophenyl-beta-D-mannopyranoside
-
-
0.5
p-nitrophenyl-beta-D-mannopyranoside
-
-
1.3
p-nitrophenyl-beta-D-mannopyranoside
-
-
1.43
p-nitrophenyl-beta-D-mannopyranoside
-
-
2.2
p-nitrophenyl-beta-D-mannopyranoside
-
-
10
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W200A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
10
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W645A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
11
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme D199A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.12
p-nitrophenyl-beta-D-mannoside
-
-
0.29
p-nitrophenyl-beta-D-mannoside
-
enzyme from endosperm
0.29
p-nitrophenyl-beta-D-mannoside
-
enzyme from cotyledons
0.29
p-nitrophenyl-beta-D-mannoside
-
enzyme from cotyledons
0.29
p-nitrophenyl-beta-D-mannoside
-
isozyme GM-1
0.32
p-nitrophenyl-beta-D-mannoside
-
-
0.34
p-nitrophenyl-beta-D-mannoside
-
isozyme OT-1
0.7
p-nitrophenyl-beta-D-mannoside
-
lysosomal, acidic enzyme
0.83
p-nitrophenyl-beta-D-mannoside
-
enzyme from endosperm
1.1
p-nitrophenyl-beta-D-mannoside
-
in sodium citrate buffer (100 mM, pH 3)
1.6
p-nitrophenyl-beta-D-mannoside
Polyporus sulfureus
-
-
1.78
p-nitrophenyl-beta-D-mannoside
-
isozyme GM-2
2
p-nitrophenyl-beta-D-mannoside
-
about
2.1
p-nitrophenyl-beta-D-mannoside
-
-
2.4
p-nitrophenyl-beta-D-mannoside
-
-
2.9
p-nitrophenyl-beta-D-mannoside
-
-
5
p-nitrophenyl-beta-D-mannoside
-
nonlysosomal, neutral enzyme
6.5
phenyl beta-D-mannoside
-
-
7.1
phenyl beta-D-mannoside
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.00183 - 214.4
2,4-dinitrophenyl-beta-D-mannopyranoside
322.25 - 323.41
4-nitrophenyl beta-D-fucopyranoside
13.5 - 571
4-nitrophenyl beta-D-galactopyranoside
25.5 - 439
4-nitrophenyl beta-D-glucopyranoside
1.6 - 50.99
4-nitrophenyl beta-D-mannopyranoside
0.5
4-nitrophenyl-beta-D-glucopyranose
pH 4.0, 30°C
0.033 - 3.1
4-nitrophenyl-beta-D-mannopyranose
0.1
4-nitrophenyl-beta-mannopyranose
-
pH 7.0, 37°C, mutant enzyme E215A
2.17
4-O-beta-D-mannopyranosyl-D-mannopyranose
-
pH 7.0, 37°C, wild-type enzyme
0.053
beta-D-mannosyl-1,4-beta-D-glucoside
-
pH 7.0, 37°C, wild-type enzyme
166.7
Mannotriose
-
pH 7.0, 37°C, wild-type enzyme
67.8
p-nitrophenyl beta-D-mannopyranoside
-
-
0.0097 - 157
p-nitrophenyl-beta-D-mannopyranoside
additional information
additional information
-
as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the turnover number of 167 per s is only an estimate
-
0.00183
2,4-dinitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.008
2,4-dinitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.85
2,4-dinitrophenyl-beta-D-mannopyranoside
-
mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
214.4
2,4-dinitrophenyl-beta-D-mannopyranoside
-
wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
322.25
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, wild-type
323.41
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
13.5
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, wild-type enzyme
14.2
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
66.5
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
571
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme D206N
25.5
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
34.4
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, wild-type enzyme
227.64
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, wild-type
252
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme D206N
349.62
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
439
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
1.6
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
4.2
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, wild-type enzyme
6.8
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
20.69
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
45
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme D206N
50.99
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, wild-type
0.033
4-nitrophenyl-beta-D-mannopyranose
-
pH 7.0, 37°C, wild-type enzyme
3.1
4-nitrophenyl-beta-D-mannopyranose
pH 4.0, 30°C
0.0097
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.045
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme D199A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.092
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.25
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.478
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W645A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.886
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W395A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
3.5
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W198A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
6.08
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W395A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
15.9
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme Y537A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
20.38
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme W198A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
26.85
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme Y537A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
34.43
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme Q646A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
128.1
p-nitrophenyl-beta-D-mannopyranoside
-
wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
157
p-nitrophenyl-beta-D-mannopyranoside
-
wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
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Ceratonia siliqua, Medicago sativa
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Purification and properties of beta-mannosidase from malted barley
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Lysosomal beta-D-mannosidase of rat liver
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Rattus norvegicus
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1973
Homo sapiens, Mus musculus
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Bos taurus
-
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-
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beta-Mannosidase from Thermotoga species
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beta-Mannosidase (EC 3.2.1.25) activity during and following germination of tomato (Lycopersicon esculentum Mill.) seeds. Purification, cloning and characterization
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Acid glycosidases from hen oviduct and egg albumen
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Cellvibrio mixtus
brenda
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26-35
2005
Aplysia fasciata
brenda
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Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase
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399
77-90
2006
Hordeum vulgare
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Production of thermostable beta-mannosidase by a strain of Thermoascus aurantiacus: Isolation, partial purification and characterization of the enzyme
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40
969-975
2007
Thermoascus aurantiacus
-
brenda
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Glycolytic and chitinolytic activities of Phlebotomus papatasi (Diptera: Psychodidae) from diverse ecological habitats
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54
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2007
Phlebotomus papatasi
brenda
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Mannose foraging by Bacteroides thetaiotaomicron: structure and specificity of the beta-mannosidase, BtMan2A
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282
11291-11299
2007
Bacteroides thetaiotaomicron
brenda
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Development of an efficient production method for beta-mannosidase by the creation of an overexpression system in Aspergillus aculeatus
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45
142-147
2007
Aspergillus aculeatus, Aspergillus aculeatus F-50
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