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EC Tree
IUBMB Comments The enzyme cleaves acyl-[acyl-carrier-protein] species with acyl chains of 6-16 carbon atoms although it appears to demonstrate a preference for the unacylated acyl-carrier protein (ACP) and short-chain ACPs over the medium- and long-chain species . Deletion of the gene encoding this enzyme abolishes ACP prosthetic-group turnover in vivo . Activation of apo-ACP to form the holoenzyme is carried out by EC 2.7.8.7, holo-[acyl-carrier-protein] synthase.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
acyl carrier protein phosphodiesterase, acp phosphodiesterase, 4'-phosphopantetheine hydrolase, acp hydrolyase,
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4'-phosphopantetheine hydrolase
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ACP phosphodiesterase
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acyl carrier protein phosphodiesterase
holofatty acid synthetase hydrolase
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AcpH
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acyl carrier protein phosphodiesterase
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acyl carrier protein phosphodiesterase
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acyl carrier protein phosphodiesterase
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acyl carrier protein phosphodiesterase
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holo-[acyl-carrier protein] + H2O = 4'-phosphopantetheine + apo-[acyl-carrier protein]
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hydrolysis of phosphoric ester
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holo-[acyl-carrier protein] 4'-pantetheine-phosphohydrolase
The enzyme cleaves acyl-[acyl-carrier-protein] species with acyl chains of 6-16 carbon atoms although it appears to demonstrate a preference for the unacylated acyl-carrier protein (ACP) and short-chain ACPs over the medium- and long-chain species [3]. Deletion of the gene encoding this enzyme abolishes ACP prosthetic-group turnover in vivo [3]. Activation of apo-ACP to form the holoenzyme is carried out by EC 2.7.8.7, holo-[acyl-carrier-protein] synthase.
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acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O
?
acyl-carrier protein from Escherichia coli fatty acid synthesis pathway + H2O
?
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complete conversion
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?
acyl-carrier-protein + H2O
4'-phosphopantetheine + apo-[acyl-carrier-protein]
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?
bis-4-nitrophenyl phosphate + H2O
?
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?
Clostridium butyricum acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein acyl-carrier protein
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?
E. coli acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein of acyl-carrier protein
ethyldethia-acyl-carrier protein + H2O
? + apoprotein
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?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
peptidyl carrier protein domain of non-ribosomal peptide synthase + H2O
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complete conversion
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?
thymidine 5-monophosphate 4-nitrophenyl ester + H2O
?
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?
additional information
?
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2',3'-cAMP + H2O
3'-AMP
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?
2',3'-cAMP + H2O
3'-AMP
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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CoA donates 4'-phosphopantetheine as prosthetic group
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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ACP phosphodiesterase activity is not essential for growth, although it is required for turnover of the acyl carrier protein prosthetic group in vivo
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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cleavage of acyl carrier protein species with acyl chains of 6-16 carbon atoms and is active on some non-native acyl carrier protein species. Preference for inacylated acyl carrier proteins and short chain acyl carrier proteins over the medium and long chain species
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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CoA donates 4'-phosphopantetheine as prosthetic group
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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?
acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O
?
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partial conversion
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?
acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O
?
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partial conversion
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?
E. coli acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein of acyl-carrier protein
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?
E. coli acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein of acyl-carrier protein
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no activity on large peptides of E. coli acyl-carrier-protein
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?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
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no activity on coenzyme A
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?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
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1 mol holo-fatty acid synthetase contains 1 mol 4'-phosphopantetheine
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?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
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specific for rat liver holo-fatty acid synthetase, no activity on pigeon liver holo-fatty acid synthetase
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?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
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?
additional information
?
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AcpH readily removes the N-pentylpantothenamide-modified prosthetic group of acyl carrier proteinboth in vivo and in vitro
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?
additional information
?
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AcpH does not hydrolyze 3',5'-cAMP
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?
additional information
?
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acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH
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?
additional information
?
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AcpH does not hydrolyze 3',5'-cAMP
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?
additional information
?
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acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH
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?
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acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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CoA donates 4'-phosphopantetheine as prosthetic group
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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ACP phosphodiesterase activity is not essential for growth, although it is required for turnover of the acyl carrier protein prosthetic group in vivo
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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CoA donates 4'-phosphopantetheine as prosthetic group
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?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
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?
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Co2+
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required, or other divalent cations
Fe2+
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required, or other divalent cations
Zn2+
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required, or other divalent cations
Mg2+
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Mg2+
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required, or other divalent cations
Mg2+
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required, or other divalent cations
Mn2+
required
Mn2+
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required, or other divalent cations
Mn2+
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increased activity in combination with Mg2+
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Triton X-100
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biphasic, optimal stimulation at 1 mM
2-mercaptoethanol
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required
2-mercaptoethanol
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required
dithiothreitol
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required
dithiothreitol
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required
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0.0017
acyl-carrier protein
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0.4
bis-4-nitrophenyl phosphate
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0.21
bis-4-nitrophenyl phosphate
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UniProt
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brenda
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brenda
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aggregates upon overexpression in vivo or concentration in vitro
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brenda
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25000
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x * 25000, SDS-PAGE
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monomer
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about half of the soluble fraction of AcpH is obtained as monomeric protein
monomer
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about half of the soluble fraction of AcpH is obtained as monomeric protein
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D24N
low level of activity
H6Q
low level of activity
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60
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loss of activity after 15 min in the absence of SDS
90
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stable in the presence of 1% SDS
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90°C, 1% SDS, 10 min, 20% loss of activity
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-20oC, dithiothreitol, several months, no loss of activity
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-20oC, under nitrogen, dithiothreitol or 2-mercaptoethanol, long periods, no loss of activity
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AcpH is stable enough that it can be concentrated and stored at concentrations up to 1 mg/ml without causing severe aggregation
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1 mol holo-fatty acid synthetase contains 1 mol 4'-phosphopantetheine
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4'-phosphopantetheine is periodically removed from and replaced on the holo-fatty acid synthetase
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Ni2+-NTA resin column chromatography and Superdex-75 gel filtration
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partial, amino acid composition
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partial
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expressed in Escherichia coli BL21(DE3) cells
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refolding in purification step using Profoldin soluble protein folding column
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Vagelos, P.R.; Larrabee, A.R.
Acyl carrier protein. IX. Acyl carrier protein hydrolase
J. Biol. Chem.
242
1776-1781
1967
Escherichia coli
brenda
Burton, D.N.; Sobhy, C.; Jenik, R.A.; Porter, J.W.
Interconversion of apo- and holofatty acid synthetases of rat and pigeon liver
Methods Enzymol.
62
249-262
1979
Rattus norvegicus
brenda
Sobhy, C.
Regulation of fatty acid synthetase activity. The 4'-phosphopantetheine hydrolase of rat liver
J. Biol. Chem.
254
8561-8566
1979
Rattus norvegicus
brenda
Fischl, A.S.; Kennedy, E.P.
Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli
J. Bacteriol.
172
5445-5449
1990
Escherichia coli
brenda
Burton, D.N.; Haavik, A.G.; Porter, J.W.
Comparative studies of the rat and pigeon liver fatty acid synthetases
Arch. Biochem. Biophys.
126
141-154
1968
Rattus norvegicus, Sus scrofa
brenda
Tweto, J.; Liberati, M.; Larrabee, A.R.
Protein turnover and 4'-phosphopantetheine exchange in rat liver fatty acid synthetase
J. Biol. Chem.
246
2468-2471
1971
Rattus norvegicus
brenda
Kim, M.; Qureshi, A.A.; Jenik, R.A.; Lornitzo, F.A.; Porter, J.W.
Apo- and holofatty acid synthetases from pigeon liver
Arch. Biochem. Biophys.
181
580-590
1977
Sus scrofa
brenda
Thomas, J.; Cronan, J.E.
The enigmatic acyl carrier protein phosphodiesterase of Escherichia coli: genetic and enzymological characterization
J. Biol. Chem.
280
34675-34683
2005
Escherichia coli
brenda
Thomas, J.; Rigden, D.J.; Cronan, J.E.
Acyl carrier protein phosphodiesterase (AcpH) of Escherichia coli is a non-canonical member of the HD phosphatase/phosphodiesterase family
Biochemistry
46
129-136
2007
Escherichia coli (P21515), Escherichia coli
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Thomas, J.; Cronan, J.E.
Antibacterial activity of N-pentylpantothenamide is due to inhibition of coenzyme A synthesis
Antimicrob. Agents Chemother.
54
1374-1377
2010
Escherichia coli
brenda
Murugan, E.; Kong, R.; Sun, H.; Rao, F.; Liang, Z.X.
Expression, purification and characterization of the acyl carrier protein phosphodiesterase from Pseudomonas aeruginosa
Protein Expr. Purif.
71
132-138
2010
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO-1
brenda
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