Information on EC 3.1.3.41 - 4-nitrophenylphosphatase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.1.3.41
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RECOMMENDED NAME
GeneOntology No.
4-nitrophenylphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate
show the reaction diagram
K-p-nitrophenylphosphatase is a partial reaction of the Na,K-ATPase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminobenzoate degradation
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Microbial metabolism in diverse environments
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degradation of aromatic, nitrogen containing compounds
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SYSTEMATIC NAME
IUBMB Comments
4-nitrophenylphosphate phosphohydrolase
A number of other substances, including phenyl phosphate, 4-nitrophenyl sulfate, acetyl phosphate and glycerol phosphate, are not substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-68-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NRRL-3, ATCC 9029
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activates
KCl
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, gradually inhibits ATPase activity of the enzyme
Mn2+
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activates; activity stimulating
NaCl
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase, increases stimulation by other agents, e.g. KCl, oligomycin, and ATP, stimulation mechanism and kinetics, is also required for ATPase activity of the enzyme
NH4+
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stimulates 4-nitrophenyl phosphatase activity
additional information
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neither Ba2+ nor Sr2+ is able to activate pNPPase activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
choline chloride
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synergistic with ouabain
Ethacrynic acid
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synergistic with ouabain
ethane diamine
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inhibits Mn2+ stimulation of pNPPase (as well as K+ and Mg2+ stimulation)
Fluorescein isothiocyanate
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i.e. FITC, inhibits both the ATPase and 4-nitrophenyl phosphatase activities of the enzyme, fluorescence labeling, inhibition depends on pH
NaCl
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synergistic with ouabain
o-vanadate
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inhibits the 4-nitrophenyl phosphatase activity
orthovanadate
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complete inhibition of 4-nitrophenyl phosphatase activity, synergistic with ouabain
Ouabain
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80% inhibition of 4-nitrophenyl phosphatase activity, synergistic with other inhibitors, overview
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
oligomycin
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.31 - 1.72
4-nitrophenyl phosphate
1.33
K+
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pH 7.5, 25C, 21% salinity
2.09
Mg2+
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pH 7.5, 25C, 21% salinity
11.4
NH4+
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pH 7.5, 25C, 21% salinity
additional information
additional information
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kinetics in freshwater and 21% salinity, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
108
4-nitrophenyl phosphate
Aspergillus niger
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pH 8.0, 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018 - 0.5
ATP
0.0022
o-vanadate
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pH 7.8, 37C, inhibition of 4-nitrophenyl phosphatase activity
0.305 - 0.762
Ouabain
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
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4-nitrophenyl phosphatase activity of Na+/K+-ATPase in microsomes
0.034
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enzyme isolated from brain, at 37C
0.127 - 175
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4-nitrophenyl phosphatase activity in different brain regions of high and low rearing subgroups of rats, overview
123.6
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4-nitrophenyl phosphatase activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 8.4
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assay at pH 6.4, pH 7.4, and at pH 8.4
7.4
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assay at
7.8
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assay at, 4-nitrophenyl phosphatase activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially by microsome preperation
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli M15 cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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mutants deficient in the 4-nitrophenyl phosphatase PHO13 resist growth inhibition on D-xylose
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