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IUBMB Comments A number of other substances, including phenyl phosphate, 4-nitrophenyl sulfate, acetyl phosphate and glycerol phosphate, are not substrates.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
p-nitrophenylphosphatase, p-nitrophenyl phosphatase, pnppase, k+-phosphatase, p-nppase, 4-nitrophenylphosphatase, para-nitrophenyl phosphatase, k+-p-nitrophenyl phosphatase, tpnppase,
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K+-p-nitrophenyl phosphatase
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p-nitrophenyl phosphatase
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para-nitrophenyl phosphatase
p-nitrophenylphosphatase
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p-nitrophenylphosphatase
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p-nitrophenylphosphatase
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p-nitrophenylphosphatase
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para-nitrophenyl phosphatase
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para-nitrophenyl phosphatase
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PNPPase
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4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate
K-p-nitrophenylphosphatase is a partial reaction of the Na,K-ATPase
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hydrolysis of phosphoric ester
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4-nitrophenylphosphate phosphohydrolase
A number of other substances, including phenyl phosphate, 4-nitrophenyl sulfate, acetyl phosphate and glycerol phosphate, are not substrates.
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
ATP + H2O
ADP + phosphate
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diphosphate + H2O
2 phosphate
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p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
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additional information
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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high and low rearing subgroups of rats selected in the open field differ in the activity of K+-stimulated p-nitrophenylphosphatase in the hippocampus, overview
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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additional information
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the canine Na+/K+-ATPase shows 4-nitrophenyl phosphatase activity
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additional information
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the Kdp-ATPase, i.e. KdpA, the potassium channel part of the KdpFABC complex, of Escherichia coli shows 4-nitrophenyl phosphatase activity
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additional information
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the freshwater shrimp Na+/K+-ATPase shows 4-nitrophenyl phosphatase activity
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additional information
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the rat Na+/K+-ATPase shows 4-nitrophenyl phosphatase activity
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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high and low rearing subgroups of rats selected in the open field differ in the activity of K+-stimulated p-nitrophenylphosphatase in the hippocampus, overview
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KCl
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, gradually inhibits ATPase activity of the enzyme
NaCl
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase, increases stimulation by other agents, e.g. KCl, oligomycin, and ATP, stimulation mechanism and kinetics, is also required for ATPase activity of the enzyme
NH4+
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stimulates 4-nitrophenyl phosphatase activity
additional information
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neither Ba2+ nor Sr2+ is able to activate pNPPase activity
K+
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dependent
K+
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required, stimulates the 4-nitrophenyl phosphatase activity
K+
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stimulates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase
Mg2+
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Mg2+
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activity stimulating
Mg2+
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2 mM, 120% of initial activity
Mg2+
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the 4-nitrophenyl phosphatase activity is dependent on Mg2+
Mg2+
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required, stimulates the 4-nitrophenyl phosphatase activity
Mn2+
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activates
Mn2+
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activity stimulating
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arsenate
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competitive. 10 mM, 39% residual activity
choline chloride
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synergistic with ouabain
Ethacrynic acid
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synergistic with ouabain
ethane diamine
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inhibits Mn2+ stimulation of pNPPase (as well as K+ and Mg2+ stimulation)
Fluorescein isothiocyanate
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i.e. FITC, inhibits both the ATPase and 4-nitrophenyl phosphatase activities of the enzyme, fluorescence labeling, inhibition depends on pH
NaCl
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synergistic with ouabain
o-vanadate
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inhibits the 4-nitrophenyl phosphatase activity
orthovanadate
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complete inhibition of 4-nitrophenyl phosphatase activity, synergistic with ouabain
Ouabain
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80% inhibition of 4-nitrophenyl phosphatase activity, synergistic with other inhibitors, overview
phosphate
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competitive. 10 mM, 58% residual activity
vanadate
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10 mM, 63% residual activity
additional information
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not inhibitory: EDTA, calcium, copper, cobalt, levamisole, and 4-hydroxymercuribenzoate
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ATP
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stimulates the 4-nitrophenyl phosphatase activity at concentrations of 0.001-0.030 mM, but inhibits it at concentrations above 0.5 mM, mechanism
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oligomycin
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl
ATP
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activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, cannot be substituted by nonhydrolyzable ATP analogues for stimulation
ATP
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stimulates the 4-nitrophenyl phosphatase activity 3.5fold at concentrations of 0.001-0.030 mM, but inhibits it at concentrations above 0.5 mM, mechanism
additional information
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stimulation mechanism and kinetics
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additional information
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not influenced by glutamate, alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid, kainite, or N-methyl-D-aspartate
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Anaphylaxis
Disruption of Kupffer cells during systemic anaphylaxis in the mouse. I. Properties and distribution of heat- and formaldehyde-stable liver acid p-nitrophenylphosphatase.
Anaphylaxis
Disruption of Kupffer cells during systemic anaphylaxis in the mouse. II. Changes in tissue and plasma heat- and formaldehyde-stable acid p-nitrophenylphosphatase.
Carcinoma, Ehrlich Tumor
Further investigations on the p-nitrophenylphosphatase activity of intact Ehrlich ascites tumor cells.
Carcinoma, Ehrlich Tumor
Mg2+- or Mn2+-dependent p-nitrophenylphosphatase activity is present in Ehrlich ascites tumor cells.
Carcinoma, Ehrlich Tumor
On the pH- and temperature dependence of the p-nitrophenylphosphatase activity of intact Ehrlich ascites tumor cells.
Carcinoma, Ehrlich Tumor
The effect of SH-blocking agents on the p-nitrophenylphosphatase activity of intact Ehrlich ascites tumor cells.
Glioma
Alterations of membrane integrity and cellular constituents by arachidonic acid in neuroblastoma and glioma cells.
Hyperalgesia
Involvement of NIPSNAP1, a neuropeptide nocistatin-interacting protein, in inflammatory pain.
Hypokalemia
Hypokalemia decreases Na(+)-K(+)-ATPase alpha 2- but not alpha 1-isoform abundance in heart, muscle, and brain.
Infections
Changes in Na,K-ATPase, sodium ion, and glucose transport in isolated enterocytes in an experimental model of malabsorption.
Lymphoma
A reinterpretation of phosphatase-N.
Muscular Dystrophy, Duchenne
4-Nitrophenylalkaline phosphatase inhibition in muscular dystrophy.
Neoplasms
Further investigations on the p-nitrophenylphosphatase activity of intact Ehrlich ascites tumor cells.
Neoplasms
Mg2+- or Mn2+-dependent p-nitrophenylphosphatase activity is present in Ehrlich ascites tumor cells.
Neoplasms
On the pH- and temperature dependence of the p-nitrophenylphosphatase activity of intact Ehrlich ascites tumor cells.
Neoplasms
The effect of SH-blocking agents on the p-nitrophenylphosphatase activity of intact Ehrlich ascites tumor cells.
Neuroblastoma
Alterations of membrane integrity and cellular constituents by arachidonic acid in neuroblastoma and glioma cells.
Starvation
Effects of starvation, feeding, and time of day on the activity of proton transport adenosine triphosphatase in the parietal cells of the mouse gastric glands.
Stomach Ulcer
PYY-Tag transgenic mice displaying abnormal (H+-K+)ATPase activity and gastric mucosal barrier impairment.
Thyroid Neoplasms
Changes in localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in human thyroid carcinoma cells.
Uremia
The effect of experimental uremia on potassium-activated phosphatase from erythrocyte and cardiac membranes.
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0.06 - 1.72
4-nitrophenyl phosphate
0.11
ATP
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K0.5 value, Hill coefficient 1.6, pH 3.5, 37°C
0.084
diphosphate
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K0.5 value, Hill coefficient 2.3, pH 3.5, 37°C
1.33
K+
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pH 7.5, 25°C, 21% salinity
2.09
Mg2+
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pH 7.5, 25°C, 21% salinity
11.4
NH4+
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pH 7.5, 25°C, 21% salinity
additional information
additional information
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kinetics in freshwater and 21% salinity, overview
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0.06
4-nitrophenyl phosphate
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K0.5 value, Hill coefficient 1.2, pH 3.5, 37°C
1.31
4-nitrophenyl phosphate
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pH 7.5, 25°C, 21% salinity
1.72
4-nitrophenyl phosphate
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pH 7.5, 25°C, fresh water
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108
4-nitrophenyl phosphate
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pH 8.0, 30°C
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0.6
arsenate
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pH 3.5, 37°C
0.0022
o-vanadate
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pH 7.8, 37°C, inhibition of 4-nitrophenyl phosphatase activity
1.8
phosphate
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pH 3.5, 37°C
0.018
ATP
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pH 7.5, 25°C
0.5
ATP
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about, pH 7.8, 37°C, inhibition of 4-nitrophenyl phosphatase activity
0.305
Ouabain
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pH 7.5, 25°C, 21% salinity
0.762
Ouabain
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pH 7.5, 25°C, fresh water
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0.002
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4-nitrophenyl phosphatase activity of Na+/K+-ATPase in microsomes
0.034
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enzyme isolated from brain, at 37°C
0.127 - 175
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4-nitrophenyl phosphatase activity in different brain regions of high and low rearing subgroups of rats, overview
123.6
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4-nitrophenyl phosphatase activity
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6.4 - 8.4
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assay at pH 6.4, pH 7.4, and at pH 8.4
7.8
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assay at, 4-nitrophenyl phosphatase activity
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37
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assay at, 4-nitrophenyl phosphatase activity
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NRRL-3, ATCC 9029
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SwissProt
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male Wistar rats
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medulla
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additional information
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mutants deficient in the 4-nitrophenyl phosphatase PHO13 resist growth inhibition on D-xylose
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4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months
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partially by microsome preperation
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partially by microsome preperation
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partially by microsome preperation
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expressed in Escherichia coli M15 cells
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expression is induced by presence of phosphate
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Attias, J.; Durand, H.
Further characterization of a specific p-nitrophenylphosphatase from baker's yeast
Biochim. Biophys. Acta
321
561-568
1973
Saccharomyces cerevisiae
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Attias, J.; Bonnet, J.L.
A specific alkaline p-nitrophenylphosphatase activity from baker's yeast
Biochim. Biophys. Acta
268
422-430
1972
Saccharomyces cerevisiae
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Yang, J.; Sunder, S.D.; Schweingruber, M.E.
Characterisation of the specific p-nitrophenylphosphatase gene and protein of Schizosaccharomyces pombe
Eur. J. Biochem.
198
493-497
1991
Schizosaccharomyces pombe
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Versaw, W.K.; Bevins, M.A.; Markwell, J.
Purification and properties of a 4-nitrophenylphosphatase from Aspergillus niger
Arch. Biochem. Biophys.
287
85-90
1991
Aspergillus niger
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Kuznetsova, E.; Proudfoot, M.; Sanders, S.A.; Reinking, J.; Savchenko, A.; Arrowsmith, C.H.; Edwards, A.M.; Yakunin, A.F.
Enzyme genomics: Application of general enzymatic screens to discover new enzymes
FEMS Microbiol. Rev.
29
263-279
2005
Thermoplasma acidophilum (Q9HJW8)
brenda
Bramkamp, M.; Gassel, M.; Altendorf, K.
FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coli
Biochemistry
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4559-4567
2004
Escherichia coli
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Alves, R.; Barbosa de Carvalho, J.G.; Benedito, M.A.
High and low rearing subgroups of rats selected in the open field differ in the activity of K+-stimulated p-nitrophenylphosphatase in the hippocampus
Brain Res.
1058
178-182
2005
Rattus norvegicus
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Mendonca, N.N.; Masui, D.C.; McNamara, J.C.; Leone, F.A.; Furriel, R.P.
Long-term exposure of the freshwater shrimp Macrobrachium olfersii to elevated salinity: effects on gill (Na+,K+)-ATPase alpha-subunit expression and K+-phosphatase activity
Comp. Biochem. Physiol. A
146
534-543
2007
Macrobrachium olfersii
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Homareda, H.; Ushimaru, M.
Stimulation of p-nitrophenylphosphatase activity of Na+/K+-ATPase by NaCl with oligomycin or ATP
FEBS J.
272
673-684
2005
Canis lupus familiaris
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Gatto, C.; Arnett, K.L.; Milanick, M.A.
Divalent cation interactions with Na,K-ATPase cytoplasmic cation sites: implications for the para-nitrophenyl phosphatase reaction mechanism
J. Membr. Biol.
216
49-59
2007
Canis lupus familiaris
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Durfinova, M.; Brechtlova, M.; Liska, B.; Baroskova, Z.
K(+)-p-nitrophenylphosphatase activity in rat brain and liver
Physiol. Res.
58
121-126
2009
Rattus norvegicus
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Van Vleet, J.H.; Jeffries, T.W.; Olsson, L.
Deleting the para-nitrophenyl phosphatase (pNPPase), PHO13, in recombinant Saccharomyces cerevisiae improves growth and ethanol production on D-xylose
Metab. Eng.
10
360-369
2008
Saccharomyces cerevisiae
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Conto, M.B.; Venditti, M.A.
In vitro studies of the influence of glutamatergic agonists on the Na+,K+-ATPase and K+-p-nitrophenylphosphatase activities in the hippocampus and frontal cortex of rats
J. Negat. Results Biomed.
11
12
2012
Rattus norvegicus
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Guo, Z.; Wang, F.; Shen, T.; Huang, J.; Wang, Y.; Ji, C.
Crystal structure of thermostable p-nitrophenylphosphatase from Bacillus stearothermophilus (Bs-TpNPPase)
Protein Pept. Lett.
21
483-489
2014
Geobacillus stearothermophilus (Q8L1N9), Geobacillus stearothermophilus
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Sato, V.S.; Galdiano Junior, R.F.; Rodrigues, G.R.; Lemos, E.G.; Pizauro Junior, J.M.
Kinetic characterization of a novel acid ectophosphatase from Enterobacter asburiae
J. Microbiol.
54
106-113
2016
Enterobacter asburiae
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