Information on EC 3.1.3.24 - sucrose-phosphate phosphatase

-

2.5% of the activity with sucrose 6-phosphate

-

?

D-fructose 6-phosphate + H2O

D-fructose + phosphate

-

1.9% of the activity with sucrose 6-phosphate

-

?

fructose 1,6-diphosphate + H2O

?

-

1.5% of the activity with sucrose 6-phosphate

-

?

p-nitrophenyl phosphate + H2O

4-nitrophenol + phosphate

phosphoenolpyruvate + H2O

pyruvate + phosphate

-

5.0% of the activity with sucrose 6-phosphate

-

?

sucrose 6-phosphate + H2O

sucrose + phosphate

13 entries

sucrose phosphate + H2O

sucrose + phosphate

-

114200

-

114200

?

UDP + H2O

?

-

1.7% of the activity with sucrose 6-phosphate

-

?

additional information

?

-

11 entries

sucrose 6-phosphate + H2O

sucrose + phosphate

additional information

?

-

Mg2+

13 entries

Mn2+

additional information

4-nitrophenyl-alpha-L-galactopyranoside

-

6-kestose

Ammonium molybdate

AMP

-

68% residual activity at 5 mM

Ca2+

cellobiose

-

D-Fructose 1-phosphate

-

62% residual activity at 5 mM

D-glucose

-

D-glucose 6-phosphate

-

67% residual activity at 5 mM

D-ribose 5-phosphate

-

9% residual activity at 5 mM

diphosphate

EDTA

F-

fluoride

5% inhibition at 2.5 mM

glucose

-

100 mM, 25% inhibition

maltose

melizitose

Mn2+

molybdate

12% inhibition at 2.5 mM

phosphate

sucrose

trehalose

-

turanose

vanadate

Zn2+

-

additional information

-

ATP

-

148% activity at 5 mM

D-fructose 1,6-bisphosphate

-

124% activity at 5 mM

0.065 - 3.5

sucrose 6-phosphate

8 entries

0.15

sucrose phosphate

-

114200

3.6 - 67.8

sucrose 6-phosphate

1 - 92.5

sucrose 6-phosphate

100

cellobiose

-

354

D-glucose

-

100

maltose

-

80 - 161

sucrose

26

trehalose

-

8

4-nitrophenyl-alpha-L-galactopyranoside

Synechocystis sp.

-

uncompetitive inhibition

0.37

Zea mays

Q9FQ11

recombinant enzyme in extract of expressing Escherichia coli

1.433

-

1250

-

purified enzyme

15.66

purified enzyme

additional information

6 - 6.5

-

114209

6.4 - 6.7

Saccharum spp.

-

6.5

6.8

-

7

-

5 - 8

-

pH 5.0: about 25% of maximal activity, pH 8.0: about 70% of maximal activity

114208

5.5 - 8

-

pH 5.5: about 50% of maximal activity, pH 8.0: about 65% of maximal activity

114209

6 - 8

Saccharum spp.

-

pH 6.0: about 20% of maximal activity, pH 8.0: about 45% of maximal activity

45

-

strain PCC 7120

SwissProt

Asclepias rotundifolia

-

-

-

-

Bryum sp.

-

-

-

-

-

-

cv. Caturra, CCCA02 and CCCA12

694409

-

Coffea canephora

var. robusta FRT64 and FRT05

694409

A7IZK6

SwissProt

-

114200, 114204, 114205

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

UniProt

-

-

Nephrolepis cordifolia

-

-

Nicotiana tabacum

no activity in Agaricus campestris

-

-

no activity in Asparagopsis taxiformis

-

-

no activity in Cystophora subfarcinata

-

-

no activity in Ecklonia radiata

-

-

no activity in Hormosira banksii

-

-

no activity in Saccharomyces cerevisiae

-

-

[Mannheimia] succiniciproducens

-

114208, 114210, 653641

-

-

-

-

-

-

-

Platycladus orientalis

-

-

-

-

-

-

-

-

-

-

Saccharum spp.

-

-

-

-

gene sppA

-

-

-

-

-

-

-

-

-

-

-

-

UniProt

-

-

-

-

germinating

Saccharum spp.

-

-

-

additional information

-

endoplasmic reticulum membrane

mitochondrion

Saccharum spp.

-

additional information

-

no activity detected in vacuole, chloroplast or mitochondria

-

114209

physiological function

Synechocystis sp. PCC 6803

9 entries

100000

-

gel filtration

115000

Pisum sativum

-

gel filtration

114202

120000

-

gel filtration

114208

26000 - 27000

native enzyme, gel filtration

28000

1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE

28034

1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE

32000

1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE

336000

-

gel filtration

46700

47213

x * 47213, DNA sequence calculation

50000

-

2 * 50000, SDS-PAGE

55000

Pisum sativum

-

2 * 55000, SDS-PAGE

114202

84000

-

4 * 84000, SDS-PAGE

90000

gel filtration

?

x * 47213, DNA sequence calculation

dimer

homotetramer

-

4 * 84000, SDS-PAGE

monomer

1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE

-

-

enzyme alone and in complex with various ligands, protein consists of a core domain containing the catalytic site and a smaller cap domain that contains a glucose binding site

-

666577

additional information

5.2

Saccharum spp.

-

20°C, 15 min, 97% loss of activity

114204

6

-

unstable below, bovine serum albumin reduces loss of activity

114200

100

Saccharum spp.

-

2 min, complete loss of activity

20

Saccharum spp.

-

pH 5.2, 15 min, 97% loss of activity

40

Saccharum spp.

-

10 min, complete loss of activity

-15�C, partially purified enzyme, 50% loss of activity after 7-8 days

-20°C, 10 mM HEPES buffer, pH 7, 50% glycerol, 1 mM dithiothreitol, stable

Pisum sativum

-

114202

-

25000fold, to homogeneity

-

partial

recombinant enzyme from Escherichia coli partially, and native for strain PCC 7120 3260fold

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)-pLysS by nickel affinity chromatography, ultrafiltration, anion exchange chromatography, and gel filtration

-

-

-

DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha as His-tagged enzyme

expression in Escherichia coli