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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor
Synonyms
alkaline ribonuclease, rnase m5, ribonuclease m5,
more
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5S ribosomal maturation nuclease
5S ribosomal RNA maturase
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5S ribosomal RNA maturation endonuclease
alkaline ribonuclease
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nuclease, ribosomal ribonucleate maturation endoribo-
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ribosomal RNA maturation endonuclease
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5S ribosomal maturation nuclease
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5S ribosomal maturation nuclease
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5S ribosomal RNA maturation endonuclease
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5S ribosomal RNA maturation endonuclease
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RNase M5
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endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor
endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor
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endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor
reaction scheme
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hydrolysis of phosphoric ester
hydrolysis of phosphoric ester
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hydrolysis of phosphoric ester
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5S precursor rRNA + H2O
mature 5S rRNA
5S rRNA precursor + H2O
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
artificial 5S rRNA precursor + H2O
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artificial 5S rRNA precursor substrate in complex with BL16 subunit + H2O
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RNA in complex with BL16 subunit + H2O
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additional information
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5S precursor rRNA + H2O
mature 5S rRNA
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5S precursor rRNA + H2O
mature 5S rRNA
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enzyme is responsible for maturation of 5S rRNA
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5S precursor rRNA + H2O
mature 5S rRNA
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in contrast to the Escherichia coli enzyme, the Bacillus subtilis enzyme cleaves the 5S rRNA in a double-stranded region that extends from the L5 arm in one step
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5S precursor rRNA + H2O
mature 5S rRNA
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5S precursor rRNA + H2O
mature 5S rRNA
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5S rRNA maturation
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5S rRNA precursor + H2O
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responsible for terminal maturation of 5S rRNA
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5S rRNA precursor + H2O
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responsible for terminal maturation of 5S rRNA
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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symmetrically arranged sequences are not used in the RNase M5 interaction
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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electrostatic interactions contribute about 65% to the overall binding energy
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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recognition elements in potential RNase M5 substrates mainly reside in the mature moiety of the precursor. The enzyme recognizes the overall conformation of potential substrates
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme attacks a ribonucleoprotein substrate consisting of protein BL16 in complex with the 5S rRNA precursor
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme cleaves twice in double-helical region of a 179-nucleotide precursor of 5S rRNA to yield mature 5S rRNA of 116 nucleotides plus fragments of 21 and 42 nucleotides. The conformation, not sequence, is the important feature in the locale of the cleaved bonds
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the 5'-precursor-specific segment contains one or more components recognized by RNase M5 during its interaction with the p5A substrate
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5S rRNA precursor + H2O
mature 5S rRNA + oligonucleotides
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the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
the enzyme cleaves 21 and 42 nucleotides respectively from the 5'-terminus and the 3'-terminus to yield the mature 116 nucleotide 5S rRNA
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additional information
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the artificial substrate lacking the 5'-terminal precursor segment is cleaved very much more slowly than that lacking 3' segment
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additional information
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the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution
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additional information
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mRNAs are poor or no substrates
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5S precursor rRNA + H2O
mature 5S rRNA
5S rRNA precursor + H2O
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additional information
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the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution
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5S precursor rRNA + H2O
mature 5S rRNA
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enzyme is responsible for maturation of 5S rRNA
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5S precursor rRNA + H2O
mature 5S rRNA
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5S rRNA maturation
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5S rRNA precursor + H2O
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responsible for terminal maturation of 5S rRNA
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5S rRNA precursor + H2O
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responsible for terminal maturation of 5S rRNA
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Ca2+
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divalent cation, metal or polyamine is required
K+
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activates, inhibition above 0.1 M
Mg2+
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divalent cation, metal or polyamine is required
Mn2+
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divalent cation, metal or polyamine is required
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mature 5S RNA
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from Bacillus licheniformis, E. coli or Saccharomyces carlsbergensis
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Zn2+
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inhibits in presence of Mg2+
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NH4+
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activates, inhibition above 0.1 M
putrescine
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divalent cation, metal or polyamine is required, 0.01 M putrescine is as effective as Mg2+
spermidine
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divalent cation, metal or polyamine is required, 0.3 mM is as effective as Mg2+
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Alzheimer Disease
Ribonuclease activities and distribution in Alzheimer's and control brains.
Anencephaly
Amniotic fluid alkaline ribonuclease activity: an indicator of foetal growth retardation and anencephaly.
Anencephaly
[Diagnostic value of amniotic fluid alkaline ribonuclease activity in anencephaly and IUGR]
Arthritis
[Serum ribonuclease in rheumatic disease. I. Serum alkaline ribonuclease activities in rheumatic diseases, especially in malignant rheumatoid arthritis]
Arthritis, Rheumatoid
Serum alkaline ribonuclease derived from vascular endothelial cells is raised in patients with rheumatoid vasculitis.
Arthritis, Rheumatoid
[Serum ribonuclease in rheumatic disease. I. Serum alkaline ribonuclease activities in rheumatic diseases, especially in malignant rheumatoid arthritis]
Fetal Growth Retardation
[Diagnostic value of amniotic fluid alkaline ribonuclease activity in anencephaly and IUGR]
Hepatitis
[Alkaline ribonuclease activity in the serum of patients with viral hepatitis]
Hepatitis B
Effects of recombinant leukocyte interferon on ribonuclease activities in serum in chronic hepatitis B.
Hepatitis B, Chronic
Effects of recombinant leukocyte interferon on ribonuclease activities in serum in chronic hepatitis B.
Kidney Failure, Chronic
Effect of hemodialysis on acid leukocyte-type ribonuclease, alkaline ribonuclease and polymorphonuclear elastase serum levels in patients with end-stage renal disease.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
The properties of ribonucleases isolated from urine of patients with chronic granulocytic leukemia. I. Urinary alkaline ribonuclease.
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
[The properties of ribonucleases isolated from urine of patients with chronic granulocytic leukemia (CGL). I. Urinary alkaline ribonuclease (RNase)]
Liver Cirrhosis
[Serum alkaline ribonuclease activity in patients with liver cirrhosis and metastatic liver neoplasms]
Liver Neoplasms
[Serum alkaline ribonuclease activity in patients with liver cirrhosis and metastatic liver neoplasms]
Lymphoma
Ribonucleases in mouse ascitic tumor cells during ageing in vivo and in vitro.
Malnutrition
Effects of early undernutrition and subsequent refeeding on alkaline ribonuclease activity of rat cerebrum and liver.
Malnutrition
Evaluation of urinary alkaline ribonuclease activity in the assessment of subclinical malnutrition in weanling rats.
Malnutrition
Nutritional status in alcoholics with and without chronic skeletal muscle myopathy.
Neoplasms
Alkaline ribonuclease activity in lymphoid organs of mice after injection of tumor cells.
Neoplasms
Alkaline ribonuclease and ribonuclease inhibitor in mammary gland during the lactation cycle and in the R3230AC mammary tumour.
Neoplasms
De novo synthesis of alkaline ribonuclease in tumor cells induced by the aklylating agent Trenimon.
Neoplasms
Plasma membrane associated enzymes of mammary tumours as the biochemical indicators of metastasizing capacity. Analyses of enriched plasma membrane preparations.
Neoplasms
Serum enzymes in ovarian carcinoma.
Neural Tube Defects
Amniotic fluid alkaline ribonuclease activity: an indicator of foetal growth retardation and anencephaly.
Neural Tube Defects
[Diagnostic value of amniotic fluid alkaline ribonuclease activity in anencephaly and IUGR]
Pancreatitis
Determination of ribonuclease activity in serum of patients with pancreatic necrosis. An attempt of extending the enzyme diagnosis of acute pancreatitis.
Protein Deficiency
[Response of the rat pancreas to the effects of secretin and pancreozymin under conditions of different nutrition]
Protein-Energy Malnutrition
The activity of serum ribonuclease in protein-energy malnutrition.
Rheumatic Diseases
[Serum ribonuclease in rheumatic disease. I. Serum alkaline ribonuclease activities in rheumatic diseases, especially in malignant rheumatoid arthritis]
Rheumatic Diseases
[Serum ribonuclease in rheumatic disease. II. Possible origin of alkaline ribonuclease from the vessels]
Rheumatoid Vasculitis
Serum alkaline ribonuclease derived from vascular endothelial cells is raised in patients with rheumatoid vasculitis.
Vasculitis
Immune-complex vasculitis: role of complement and IgG-Fc receptor functions.
Vasculitis
Serum alkaline ribonuclease derived from vascular endothelial cells is raised in patients with rheumatoid vasculitis.
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0.00003
RNA in complex with BL16 subunit
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0.00667
artificial 5S rRNA precursor
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0.0567
artificial 5S rRNA precursor substrate in complex with BL16 subunit
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brenda
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brenda
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brenda
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brenda
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brenda
gene rnmV
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brenda
gene yabF, proposition to rename the gene rnmV
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brenda
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15000
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15000 Da is the MW of the beta component, SDS-PAGE
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additional information
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RNase M5 complex includes two proteins. One of these proteins is ribosomal protein BL16, the other, the alpha component is required for catalysis
additional information
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15000 Da is the MW of the beta component, SDS-PAGE
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D14A
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less than 10% of wild-type activity
D56A
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less than 10% of wild-type activity
D58A
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less than 10% of wild-type activity
E10A
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less than 10% of wild-type activity
G11A
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less than 10% of wild-type activity
G31A
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less than 10% of wild-type activity
G61A
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less than 10% of wild-type activity
I52A
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60% of wild-type activity
I64A
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as active as wild-type enzyme
L107A
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less than 10% of wild-type activity
L162A
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about 25% of wild-type activity
R65A
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about 10% of wild-type activity
Y152A
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as active as wild-type enzyme
additional information
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bacteria lacking the gene rnmV do not produce mature 5S RNA, but it is nonessential for cell viability and not necessary for ribosomal function
additional information
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construction of mutant strains which shows significantly altered expression of some genes, e.g. ctsR, due to mutated RNase M5 which does not process 5S pre-rRNA, the effect on odhAB and sucCD operons is indirect, overview
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native from strain SSB306 containing a modified L6 protein, recombinant from Escherichia coli
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purification of beta-component
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RNase M5 mutant enzymes
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gene yabF, overexpression in Escherichia coli
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Sogin, M.L.; Page, B.; Pace, N.R.
Partial purification and properties of a ribosomal RNA maturation endonuclease from Bacillus subtilis
J. Biol. Chem.
252
1350-1357
1977
Bacillus subtilis, Bacillus subtilis 168
brenda
Pace, B.; Stahl, D.A.; Pace, N.R.
The catalytic element of a ribosomal RNA-processing complex
J. Biol. Chem.
259
11454-11458
1984
Bacillus subtilis
brenda
Stiekema, W.J.; Raue, H.A.; Duin, M.M.C.; Planta, R.J.
Structural features of Bacillus precursor 5S RNA involved in the interaction with RNAase M5
Nucleic Acids Res.
8
5411-5421
1980
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Q
brenda
Stahl, A.A.; Meyhack, B.; Pace, N.R.
Recognition of local nucleotide conformation in contrast to sequence by a rRNA processing endonuclease
Proc. Natl. Acad. Sci. USA
77
5644-5648
1980
Bacillus subtilis
brenda
Meyhack, B.; Pace, N.R.
Involvement of the mature domain in the in vitro maturation of Bacillus subtilis precursor 5S ribosomal RNA
Biochemistry
17
5804-5810
1978
Bacillus subtilis
brenda
Meyhack, B.; Pace, B.; Uhlenbeck, O.C.; Pace, N.R.
Use of T4 RNA ligase to construct model substrates for a ribosomal RNA maturation endonuclease
Proc. Natl. Acad. Sci. USA
75
3045-3049
1978
Bacillus subtilis
brenda
Meyhack, B.; Pace, B.; Pace, N.R.
Involvement of precursor-specific segments in the in vitro maturation of Bacillus subtilis precursor 5S ribosomal RNA
Biochemistry
16
5009-5015
1977
Bacillus subtilis
brenda
Stahl, D.A.; Pace, B.; Marsh, T.; Pace, N.R.
The ribonucleoprotein substrate for a ribosomal RNA-processing nuclease
J. Biol. Chem.
259
11448-11453
1984
Bacillus subtilis
brenda
Condon, C.; Rourera, J.; Brechemier-Baey, D.; Putzer, H.
Ribonuclease M5 has few, if any, mRNA substrates in Bacillus subtilis
J. Bacteriol.
184
2845-2849
2002
Bacillus subtilis
brenda
Condon, C.; Brechemier-Baey, D.; Beltchev, B.; Grunberg-Manago, M.; Putzer, H.
Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function
RNA
7
242-253
2001
Bacillus subtilis
brenda
Allemand, F.; Mathy, N.; Brechemier-Baey, D.; Condon, C.
The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member
Nucleic Acids Res.
33
4368-4376
2005
Geobacillus stearothermophilus
brenda
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