Information on EC 3.1.26.11 - tRNase Z

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.26.11
-
RECOMMENDED NAME
GeneOntology No.
tRNase Z
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
CAS REGISTRY NUMBER
COMMENTARY hide
98148-84-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
Clostridium spp.
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
distribution of short and long forms of tRNase Z reviewed
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
distribution of short and long forms of tRNase Z reviewed
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
tRNase Z in prokaryotes and archae, overview
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
-
Schizosaccharomyces pombe contains two candidate tRNase ZLs encoded by the essential genes sptrz1+ and sptrz2+
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
415-nt CAT mRNA + H2O
?
show the reaction diagram
-
5'-half portion of the E. coli chloramphenicol acetyltransferase mRNA
-
-
?
bis(p-nitrophenyl)phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
GENV7-Env + H2O
?
show the reaction diagram
GT7H + H2O
?
show the reaction diagram
-
complex of SPH2 with 5' half tRNA
-
-
?
GT7HM10 + H2O
?
show the reaction diagram
-
complex of SPH2 with 5' half tRNA
-
-
?
GT7HM20 + H2O
?
show the reaction diagram
-
complex of SPH2 with 5' half tRNA
-
-
?
GT7HM22 + H2O
?
show the reaction diagram
-
complex of SPH2 with 5' half tRNA
-
-
?
IM0 + H2O
?
show the reaction diagram
IM1 + H2O
?
show the reaction diagram
IM2 + H2O
?
show the reaction diagram
IM3 + H2O
?
show the reaction diagram
IT1 + H2O
?
show the reaction diagram
IT2 + H2O
?
show the reaction diagram
IT3 + H2O
?
show the reaction diagram
ITO + H2O
?
show the reaction diagram
mt-pre-tRNAArg + H2O
mt-tRNAArg + 3'-leader of tRNA
show the reaction diagram
-
-
-
-
?
mt-pre-tRNAGlu + H2O
mt-tRNAGlu + 3'-leader of tRNA
show the reaction diagram
-
-
-
-
?
mt-pre-tRNAGly + H2O
mt-tRNAGly + 3'-leader of tRNA
show the reaction diagram
-
-
-
-
?
mt-pre-tRNALeu + H2O
mt-tRNALeu + 3'-leader of tRNA
show the reaction diagram
-
-
-
-
?
mt-pre-tRNALys + H2O
mt-tRNALys + 3'-leader of tRNA
show the reaction diagram
-
-
-
-
?
mt-pre-tRNAVal + H2O
mt-tRNAVal + 3'-leader of tRNA
show the reaction diagram
-
-
-
-
?
pre-tRNA + H2O
?
show the reaction diagram
pre-tRNA-CAG-trailer + H2O
?
show the reaction diagram
-
5'-, 3'-extended chloroplast pre-tRNAPhe
-
-
?
pre-tRNA-CCA-trailer + H2O
?
show the reaction diagram
pre-tRNA-CCAOH-trailer + H2O
?
show the reaction diagram
pre-tRNA9-AspATC + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAAla + H2O
?
show the reaction diagram
pre-tRNAarg + H2O
?
show the reaction diagram
pre-tRNAArg + H2O
tRNAArg + 3'-leader of tRNA
show the reaction diagram
-
-
-
-
?
pre-tRNAArg(+L) + H2O
?
show the reaction diagram
pre-tRNAArg(-L) + H2O
?
show the reaction diagram
pre-tRNAArg(74CCA76) + H2O
?
show the reaction diagram
-
reaction kinetics for in vitro 3'-processing using engineered enzyme variants, in presence of 10 mM Mg2+ or of 0.2 mM Mn2+
-
-
?
pre-tRNAArg(74GUG76) + H2O
?
show the reaction diagram
-
cleavage of pre-tRNAArg(74GUG76) by engineered variants about 7 to more than 16fold less efficient than that of pre-tRNAArg(74CCA76)
-
-
?
pre-tRNAArg(CCA) + H2O
?
show the reaction diagram
pre-tRNAArg(CCG) + H2O
?
show the reaction diagram
pre-tRNAArg(CUG) + H2O
?
show the reaction diagram
pre-tRNAAsp + H2O
?
show the reaction diagram
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNACys + H2O
?
show the reaction diagram
pre-tRNAGlu + H2O
?
show the reaction diagram
pre-tRNAGlu-CCAN17 + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAGlu-CCUN17 + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAGlu-CUAN17 + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAGlu-UCAN17 + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAGlu-UUAN17 + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAGlu-UUUN17 + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAGly + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAHis + H2O
?
show the reaction diagram
pre-tRNAHis-AUG + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAHis-CCA + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNAIle + H2O
?
show the reaction diagram
pre-tRNALeu + H2O
?
show the reaction diagram
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNALeu(CUN) + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNALeu(UUR) + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNALys + H2O
?
show the reaction diagram
pre-tRNAMet + H2O
?
show the reaction diagram
pre-tRNAMet(CCA) + H2O
?
show the reaction diagram
pre-tRNAMet(UAG) + H2O
?
show the reaction diagram
pre-tRNAMetTrp + H2O
?
show the reaction diagram
pre-tRNAPhe + H2O
?
show the reaction diagram
pre-tRNAPhe(CCA) + H2O
?
show the reaction diagram
pre-tRNAPhe(CCG) + H2O
?
show the reaction diagram
-
tRNA from Thermotoga maritima, no cleavage
-
-
?
pre-tRNAPhe(CUA) + H2O
?
show the reaction diagram
pre-tRNAPhe(UCA) + H2O
?
show the reaction diagram
pre-tRNASer + H2O
?
show the reaction diagram
-
the enzyme catalyzes endonucleolytic tRNA 3' processing
-
-
?
pre-tRNASer(AGY) + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNASer(UCN) + H2O
?
show the reaction diagram
-
wild type
-
-
-
pre-tRNASer(UCN)7443C + H2O
?
show the reaction diagram
-
natural tRNA mutant
-
-
-
pre-tRNASer(UCN)7444U + H2O
?
show the reaction diagram
-
natural tRNA mutant
-
-
-
pre-tRNASer(UCN)7445C + H2O
?
show the reaction diagram
-
natural tRNA mutant
-
-
-
pre-tRNASer(UCN)7445G + H2O
?
show the reaction diagram
-
natural tRNA mutant
-
-
-
pre-tRNASer(UCN)7510G + H2O
?
show the reaction diagram
-
natural tRNA mutant
-
-
-
pre-tRNASer(UCN)7511G + H2O
?
show the reaction diagram
-
natural tRNA mutant
-
-
-
pre-tRNASer(UCN)7512G + H2O
?
show the reaction diagram
-
natural tRNA mutant
-
-
-
pre-tRNAThr + H2O
?
show the reaction diagram
-
-
-
-
?
pre-tRNATyr + H2O
?
show the reaction diagram
pre-tRNAVal + H2O
?
show the reaction diagram
precursor tRNASer(UGA)-M + H2O
mature tRNASer(UGA)-M
show the reaction diagram
precursor tRNASer(UGA)-M + H2O
mature tRNASer(UGA)-M + ?
show the reaction diagram
-
overexpression of untagged TRZ1, but not FLAG-tagged TRZ1, increases the abundance of mature tRNASer(UGA)-M in strain yYH1
-
-
?
precursor tRNATyr + H2O
tRNA + 3' trailer
show the reaction diagram
pretRNA35-MetCAT + H2O
?
show the reaction diagram
-
-
-
-
?
pTyrI + H2O
?
show the reaction diagram
-
pre-tRNATyr from Oenothera berteriana
-
-
?
R-A1 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-ACA19 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-ACA3 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-ASA1 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with one base-pair addition after the fifth base-pair of the acceptor stem
-
-
-
R-ASA2 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with two base-pair additions after the fifth base-pair of the acceptor stem
-
-
-
R-ASA3 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with three base-pair additions after the fifth base-pair of the acceptor stem
-
-
-
R-ASD1 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with one base-pair deletion after the third base-pair of the acceptor stem
-
-
-
R-ASD2 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with two base-pair deletions after the third base-pair of the acceptor stem
-
-
-
R-AT12A + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with one additional base-pair in the acceptor stem and one deleted base-pair in the T stem
-
-
-
R-AT12B + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with one deleted base-pair in the acceptor stem and one additional base-pair in the T stem
-
-
-
R-AT14 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with one base-pair addition in both acceptor and T stems
-
-
-
R-AT16 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with two base-pair additions in both acceptor and T stems
-
-
-
R-ATM1 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM10 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM11 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM2 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM3 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM4 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM5 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM7 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM8 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATM9 + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
R-ATW + H2O
?
show the reaction diagram
-
small pre-tRNAArg
-
-
?
R-C1 + H2O
?
show the reaction diagram
R-CCA19 + H2O
?
show the reaction diagram
R-CCA6 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-CCA8 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-CCG + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-CUA + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-G1 + H2O
?
show the reaction diagram
R-G10 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-G13 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-G15 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-G19 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-G3 + H2O
?
show the reaction diagram
R-G8 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-GCA + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-L0 + H2o
?
show the reaction diagram
-
wild type pre-tRNAArg
-
-
?
R-L3 + H2o
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-L6 + H2o
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-TSA1 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with one base-pair addition in the T stem
-
-
-
R-TSA2 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with two base-pair additions in the T stem
-
-
-
R-TSD1 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with one base-pair deletion in the T stem
-
-
-
R-TSD2 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct with two base-pair deletions in the T stem
-
-
-
R-U1 + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-UCA + H2O
?
show the reaction diagram
-
pre-tRNAArg construct
-
-
?
R-UUU + H2O
?
show the reaction diagram
-
small pre-tRNAArg construct
-
-
?
Rib3 + H2O
?
show the reaction diagram
Rib4 + H2O
?
show the reaction diagram
S-D-lactoylglutathione + H2O
?
show the reaction diagram
-
is a glyoxalase II substrate, tRNase Z has a broad substrate spectrum, is able to process a substrate belonging to a different subclass of the MBL family
-
-
?
S5L + H2O
?
show the reaction diagram
S6L + H2O
?
show the reaction diagram
S7L + H2O
?
show the reaction diagram
S8L + H2O
?
show the reaction diagram
S9L + H2O
?
show the reaction diagram
t-RNAHis48 + H2O
?
show the reaction diagram
t-RNAHis48 3'C + H2O
?
show the reaction diagram
t-RNAHis48 3'CC + H2O
?
show the reaction diagram
T7-5 + H2O
?
show the reaction diagram
-
complex of SPH2 with shorter RNA
-
-
?
T7M7 + H2O
?
show the reaction diagram
-
complex of SPH2 with RNA heptamer
-
-
?
T7M7-T3H + H2O
?
show the reaction diagram
T7M7-T7HM1 + H2O
?
show the reaction diagram
T7M7-T7HM2 + H2O
?
show the reaction diagram
T7M71 + H2O
?
show the reaction diagram
-
complex of SPH2 with RNA heptamer
-
-
?
thymidine 5'-p-nitrophenyl phosphate + H2O
p-nitrophenol + TMP
show the reaction diagram
-
-
-
-
?
thymidine-5-p-nitrophenyl phosphate + H2O
p-nitrophenol + TMP
show the reaction diagram
-
TpNPP substrate, phosphodiesterase activities of tRNase Z on small chromogenic substrates mentioned, structural features of potential model substrates indicated
-
-
?
tRNA T3 + H2O
?
show the reaction diagram
tRNA T6 + H2O
?
show the reaction diagram
tRNATyr + H2O
?
show the reaction diagram
-
wild type tRNA precursor
-
-
?
trnB-THr + H2O
?
show the reaction diagram
-
pre-tRNATHr with a 47 nucleotide 3' trailing sequence and CCA motif, no cleavage
-
-
?
trnI-Thr + H2O
?
show the reaction diagram
-
pre-tRNAThr with a 83 nucleotide 3' trailing sequence, cleavage at one or two bases downstream the discriminator base
-
-
?
trnI-Thr-CAAATG-trailer + H2O
?
show the reaction diagram
-
-
-
-
?
trnI-Thr-CCAATG-trailer + H2O
?
show the reaction diagram
-
-
-
-
?
trnI-Thr-TAAATG-trailer + H2O
?
show the reaction diagram
-
native trailer sequence
-
-
?
trnI-Thr-TCAATG-trailer + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin fusion ribosomal protein L40 mRNA + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin fusion ribosomal protein L401 mRNA + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin fusion ribosomal protein L402 mRNA + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin fusion ribosomal protein L403 mRNA + H2O
?
show the reaction diagram
-
-
-
-
?
usRNA1 + H2O
?
show the reaction diagram
usRNA10 + H2O
?
show the reaction diagram
usRNA2 + H2O
?
show the reaction diagram
usRNA3 + H2O
?
show the reaction diagram
usRNA4 + H2O
?
show the reaction diagram
usRNA5 + H2O
?
show the reaction diagram
usRNA6 + H2O
?
show the reaction diagram
usRNA7 + H2O
?
show the reaction diagram
usRNA8 + H2O
?
show the reaction diagram
usRNA9 + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ubiquitin fusion ribosomal protein L40 mRNA + H2O
?
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
with 0.2 mM, at 37C, pH 7.4, 28% relative activity when compared to Co2+
Fe2+
-
with 0.2 mM, at 37C, pH 7.4, 34% relative activity when compared to Co2+
MgCl2
-
most active at 0-0.2 mM, weak activity above 3 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bis(p-nitrophenyl)phosphate
MgCl2
-
>60 mM, in vitro
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
with 0.2 mM, at 37C, pH 7.4, 11% relative activity when compared to Co2+
additional information
-
is only active at low salt concentrations
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 22.2
bis(p-nitrophenyl)phosphate
0.000016
pre-tRNA(Arg)
-
-
-
0.0000043
pre-tRNA(Val)
-
-
-
0.000022 - 0.000132
pre-tRNAArg
-
0.00047
pre-tRNAGlu-CCUN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.00155
pre-tRNAGlu-CUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.00096
pre-tRNAGlu-UUAN17
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.000029
pre-tRNAGly
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37C
-
0.000018 - 0.000155
pre-tRNAHis
-
0.0000394
pre-tRNAHis-AUG
-
-
-
0.0000401
pre-tRNAHis-CCA
-
-
-
0.000048
pre-tRNAIle
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37C
-
0.000024
pre-tRNALeu(UUR)
-
25 mM K-MOPS, 3 mM CaCl2, at pH 6.8 and 37C
-
0.0000048
pre-tRNASer(UCN)
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000633
pre-tRNASer(UCN)7443C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000069
pre-tRNASer(UCN)7444U
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000197
pre-tRNASer(UCN)7445C
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000855
pre-tRNASer(UCN)7445G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000108
pre-tRNASer(UCN)7510G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.0000076
pre-tRNASer(UCN)7511G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.000004
pre-tRNASer(UCN)7512G
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.00044
R-ATM1
-
-
-
0.00065
R-ATM10
-
-
-
0.00068
R-ATM11
-
-
-
0.00032
R-ATM2
-
-
-
0.00059
R-ATM3
-
-
-
0.00066
R-ATM4
-
-
-
0.00057
R-ATM5
-
-
-
0.00058
R-ATM7
-
-
-
0.00052
R-ATM8
-
-
-
0.00063
R-ATM9
-
-
-
0.00041
R-ATW
-
-
-
0.00073
R-G10
-
-
-
0.0014
R-G13
-
-
-
0.001
R-G15
-
-
-
0.0012
R-G19
-
-
-
0.00085
R-G3
-
-
-
0.00078
R-G8
-
-
-
0.00083 - 0.00085
R-L0
-
0.00069
R-L3
-
-
-
0.0006
R-L6
-
-
-
0.0011
R-TASD2
-
-
-
0.0016
R-TSA2
-
-
-
0.0019
R-TSD1
-
-
-
0.00057
R-UUU
-
-
-
0.00002 - 0.00008
tRNAHis48
-
0.00009
tRNAHis48 3'+ C
-
C is added to the 3'-end of tRNA
0.00009
tRNAHis48 3'+ CC
-
CC is added to the 3'-end of tRNA
-
0.0022
trnI-Thr-CAAATG-trailer
-
the first cytosine is the primary anti-determinant for RNaseZ
-
0.0024
trnI-Thr-CCAATG-trailer
-
CCA motif inhibits RNaseZ cleavage
-
0.00097
trnI-Thr-TAAATG-trailer
-
native trailer sequence
-
0.00058
trnI-Thr-TCAATG-trailer
-
-
-
additional information
additional information
-
kinetics of tRNase Z processing in wild-type and variants shown, effects of substitutions in conserved regions on the C-side of motif II indicated, KM-values for tested variants similar to the mean wild-type KM value of 15 nM, residues involved with metal ion binding and catalysis not directly involved with substrate recognition and binding
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.67
pre-tRNA(Arg)
Homo sapiens
-
-
-
0.27
pre-tRNA(Val)
Homo sapiens
-
-
-
0.021 - 6.18
pre-tRNAArg
-
0.14
pre-tRNAGlu-CCUN17
Synechocystis sp.
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.28
pre-tRNAGlu-CUAN17
Synechocystis sp.
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
2.25
pre-tRNAGlu-UUAN17
Synechocystis sp.
-
40 mM Tris-HCl pH 7.5, 2 mM dithiothreitol
-
0.035
pre-tRNAGly
Homo sapiens
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37C
-
0.083 - 0.237
pre-tRNAHis
-
0.365
pre-tRNAHis-AUG
Drosophila melanogaster
-
-
-
0.0049
pre-tRNAHis-CCA
Drosophila melanogaster
-
-
-
0.018
pre-tRNAIle
Homo sapiens
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37C
-
0.25
pre-tRNALeu(UUR)
Homo sapiens
-
25 mM K-MOPS, 3 mM CaCl2, at pH 6.8 and 37C
-
0.07
pre-tRNASer(UCN)
Homo sapiens
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.23
pre-tRNASer(UCN)7443C
Homo sapiens
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.153
pre-tRNASer(UCN)7444U
Homo sapiens
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.012
pre-tRNASer(UCN)7445C
Homo sapiens
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.345
pre-tRNASer(UCN)7445G
Homo sapiens
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
0.05
pre-tRNASer(UCN)7510G, pre-tRNASer(UCN)7511G, pre-tRNASer(UCN)7512G
Homo sapiens
-
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin
-
additional information
additional information
Arabidopsis thaliana
-
turnover rate of AthTRZ1 eight-fold reduced if compared to tRNAse Z of Escherichia coli
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1233
pre-tRNAGly
Homo sapiens
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37C
197717
12670
pre-tRNAHis
Homo sapiens
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37C
13707
417
pre-tRNAIle
Homo sapiens
-
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37C
130630
15500
pre-tRNALeu(UUR)
Homo sapiens
-
25 mM K-MOPS, 3 mM CaCl2, at pH 6.8 and 37C
197716
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
assay at
6.5 - 8.5
-
-
7.9
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.3
-
long isoform, calculated from amino acid sequence
6.6
-
long isoform, calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
preferred expression
Manually annotated by BRENDA team
-
predominant expression during the third larval instar
Manually annotated by BRENDA team
-
preferred expression
Manually annotated by BRENDA team
-
preferred expression
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information