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5'-end-labeled 19 nucleotide single-stranded DNA + H2O
?
5'-end-labeled 29 nucleotide double-stranded DNA + H2O
?
double stranded DNA + H2O
?
double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
double-stranded DNA + H2O
?
double-stranded native DNA + H2O
5'-phosphooligonucleotides
-
endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1
-
-
ir
double-stranded nativeDNA + H2O
5'-phosphooligonucleotides
-
endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides
-
-
ir
plasmid containing AP sites + H2O
?
single-stranded DNA + H2O
5'-phosphooligonucleotides
single-stranded DNA + H2O
?
-
the enzyme cleaves apurinic/apyrimidinic sites in single-stranded DNA. DNA probe containing a tetrahydrofuranyl abasic site to mimic the natural apurinic/apyrimidinic site
-
-
?
additional information
?
-
21-mer C-Grec + H2O
?
-
-
-
-
?
21-mer C-Grec + H2O
?
-
-
-
-
?
5'-end-labeled 19 nucleotide single-stranded DNA + H2O
?
-
-
-
?
5'-end-labeled 19 nucleotide single-stranded DNA + H2O
?
-
-
-
?
5'-end-labeled 29 nucleotide double-stranded DNA + H2O
?
92% cleavage after 30 min
-
-
?
5'-end-labeled 29 nucleotide double-stranded DNA + H2O
?
92% cleavage after 30 min
-
-
?
double stranded DNA + H2O
?
-
the enzyme prefers to remove mismatched 3'-terminal nucleotides from 3'-recessed, nicked, and gapped double stranded DNA
-
-
?
double stranded DNA + H2O
?
the enzyme cleaves with good efficiency a DNA duplex containing the abasic site analogue tetrahydrofuran. The enzyme contains apurinic/apyrimidinic endonuclease activity and 3'-5' exonuclease activity
-
-
?
double stranded DNA + H2O
?
the enzyme cleaves with good efficiency a DNA duplex containing the abasic site analogue tetrahydrofuran. The enzyme contains apurinic/apyrimidinic endonuclease activity and 3'-5' exonuclease activity
-
-
?
double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
-
endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides
-
-
ir
double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
-
endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, hydrolysis of a phosphodiester bond near an alkylated base in native DNA without single breaks in the region, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1
-
-
ir
double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks
-
-
ir
double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
the enzyme cleaves sites of DNA with conserved sequence elements to both the left and the right of the cleaved bonds comprising 16 basepairs with some variability tolerated, nicking of a single and both strands, the latter in two nicking events, substrate specificity, overview, in vitro the enzyme produces mostly single-stranded nicks
-
-
ir
double-stranded DNA + H2O
?
-
Endo IV has considerably high cleaving activity to apurinic/apyrimidinic sites in ssDNA compared with that in double-stranded DNA
-
-
?
double-stranded DNA + H2O
?
-
the enzyme can cleave various apurinic/apyrimidinic site analogues in ssDNA and dsDNA. The apurinic/apyrimidinic endonuclease activity can incise DNA 5' to various apurinic/apyrimidinic site analogues, including the alkane chain Spacer and polyethylene glycol Spacer. The efficient cleavage at apurinic/apyrimidinic sites requires 2 or more normal nucleotides existing at the 5'-terminus. The enzyme also possesses 3'-exonuclease activity for removing 3'-blocking groups and normal nucleotides
-
-
?
plasmid containing AP sites + H2O
?
the enzyme has apurinic/apyrimidinic endonuclease activity, 3'-phosphatase activity, and 3'-repair diesterase activity for phosphoglycolates and trans-4-hydroxy-2-pentenal-5-phosphates
-
-
?
plasmid containing AP sites + H2O
?
the enzyme has apurinic/apyrimidinic endonuclease activity, 3'-phosphatase activity, and 3'-repair diesterase activity for phosphoglycolates and trans-4-hydroxy-2-pentenal-5-phosphates
-
-
?
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
preference for single-stranded DNA
about 150 nucleotides long
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
denatured lambda-DNA, breaks exlusively adjacent to cytosine residues
about 150 nucleotides long
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
no activity on denatured T4 DNA
about 150 nucleotides long
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
breakdown of host cell DNA occuring after T4 phage infection
-
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
preference for single-stranded DNA
about 150 nucleotides long
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
denatured lambda-DNA, breaks exlusively adjacent to cytosine residues
about 150 nucleotides long
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
no activity on denatured T4 DNA
about 150 nucleotides long
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
breakdown of host cell DNA occuring after T4 phage infection
-
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
the Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli
-
-
?
single-stranded DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
Endo IV catalyzes specific endonucleolytic cleavage of the 5' phosphodiester bond of dC in ssDNA with an efficiency dependent on the surrounding nucleotide sequence. The enzyme preferentially targets 5'-dTdCdA-3' but tolerates various combinations of individual nucleotides flanking this trinucleotide sequence. Endo IV preferentially recognizes short nucleotide sequences containing 5'-TdCdA-3', which likely accounts for the limited digestion of ssDNA by the enzyme and may be responsible in part for the indispensability of a deficiency in denB for stable synthesis of dC-substituted T4 genomic DNA
-
-
?
single-stranded DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
endonuclease IV cleaves single-stranded (ss) DNA in a dC-specific manner. The Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli
-
-
?
single-stranded DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
the enzyme cleaves single-stranded (ss) DNA in a dC-specific manner. Endo IV cleaves ssDNAs exclusively at the 5-proximal dC where a sequence comprises dC residues both at the 5 proximal and 3 proximal positions (a dCs tract-dependent cleavage). The dCs tract-dependent cleavage is efficient and occurs when a dCs tract has at least 6 bases. Some dCs tracts larger than 6 bases behave as that of 6 bases (an extended dCs tract), while some others do not. One decameric dCs tract is shown to be cleavable in a dCs tract-dependent manner, but that with 13 dCs is not. The dCs tract-dependent cleavage is enhanced by the presence of a third dC residue at least for a 6 or 7 dCs tract. In contrast to the dCs tract-dependent cleavage, a dCs tract-independent one is generally inefficient and if two modes are possible for a substrate DNA, a dCs tract-dependent mode prevails
-
-
?
additional information
?
-
-
the enzyme hydrolyzes the 3'-nucleotide in the following order: nick > 3'-blunt > 3'-recessed > single stranded DNA
-
-
?
additional information
?
-
-
DNA substrate specificity, overview
-
-
?
additional information
?
-
role for Endo IV to counteract DNA damage induced by the oxidative counterpart of UVB in Escherichia coli
-
-
?
additional information
?
-
-
role for Endo IV to counteract DNA damage induced by the oxidative counterpart of UVB in Escherichia coli
-
-
?
additional information
?
-
reaction proceeds through a synchronous bimolecular (ANDN) mechanism with reaction free energy and barrier of -3.5 and 20.6 kcal/mol.In the course of the reaction the trinuclear active site of endonuclease IV undergoes dramatic local conformational changes: shifts in the mode of coordination of both substrate and first-shell ligands
-
-
?
additional information
?
-
-
Nfo-catalyzed nucleotide incision repair and exonuclease activities can generate a single-strand gap at the 5' side of 5,6-dihydrouracil residue
-
-
?
additional information
?
-
-
Nfo-catalyzed nucleotide incision repair and exonuclease activities can generate a single-strand gap at the 5' side of 5,6-dihydrouracil residue
-
-
?
additional information
?
-
endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA
-
-
?
additional information
?
-
-
endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA
-
-
?
additional information
?
-
the enzyme also shows 3'->5' exonuclease activity
-
-
?
additional information
?
-
-
the enzyme also shows 3'->5' exonuclease activity
-
-
?
additional information
?
-
endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA
-
-
?
additional information
?
-
the enzyme also shows 3'->5' exonuclease activity
-
-
?
additional information
?
-
-
a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links
-
-
?
additional information
?
-
-
a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links
-
-
?
additional information
?
-
Tequatrovirus T4
-
endonuclease IV encoded by denB of bacteriophage T4 is implicated in restriction of deoxycytidine (dC)-containing DNA in the host Escherichia coli
-
-
?
additional information
?
-
Tequatrovirus T4
-
EndoII primarily catalyzes single-stranded nicking of DNA, 5 to 10fold less frequently double-stranded breaks are produced
-
-
?
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double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
double-stranded nativeDNA + H2O
5'-phosphooligonucleotides
-
endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides
-
-
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
additional information
?
-
double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
-
endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides
-
-
ir
double-stranded alkylated DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks
-
-
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
breakdown of host cell DNA occuring after T4 phage infection
-
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
-
breakdown of host cell DNA occuring after T4 phage infection
-
ir
single-stranded DNA + H2O
5'-phosphooligonucleotides
Tequatrovirus T4
-
the Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli
-
-
?
additional information
?
-
role for Endo IV to counteract DNA damage induced by the oxidative counterpart of UVB in Escherichia coli
-
-
?
additional information
?
-
-
role for Endo IV to counteract DNA damage induced by the oxidative counterpart of UVB in Escherichia coli
-
-
?
additional information
?
-
-
Nfo-catalyzed nucleotide incision repair and exonuclease activities can generate a single-strand gap at the 5' side of 5,6-dihydrouracil residue
-
-
?
additional information
?
-
-
Nfo-catalyzed nucleotide incision repair and exonuclease activities can generate a single-strand gap at the 5' side of 5,6-dihydrouracil residue
-
-
?
additional information
?
-
-
a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links
-
-
?
additional information
?
-
-
a His-tagged derivative of ScoA3McrA cleaves S-modified DNA and also Dcm-methylated DNA in vitro near the respective modification sites. Sco4631 cleaves DNA at multiple possible sites on either side of the S-modification. Double-strand cleavage occurs 16-28 nucleotides away from the phosphorothioate links
-
-
?
additional information
?
-
Tequatrovirus T4
-
endonuclease IV encoded by denB of bacteriophage T4 is implicated in restriction of deoxycytidine (dC)-containing DNA in the host Escherichia coli
-
-
?
additional information
?
-
Tequatrovirus T4
-
EndoII primarily catalyzes single-stranded nicking of DNA, 5 to 10fold less frequently double-stranded breaks are produced
-
-
?
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CaCl2
-
8% of the activity found compared to optimal Mg2+ concentration
CoCl2
-
can substitute for MgCl2, optimal concentration: 0.01 M, activity 27% higher than at optimal MgCl2 concentration
MgCl2
-
absolute requirement, no activity in absence, optimal concentration: 0.01 M
Ca2+
stimulates activity
Ca2+
the enzyme requires the presence of Mg2+ and, to lesser extent, Ca2+ and Mn2+ for the DNA repair activity
Co2+
the enzyme exhibits a non-linear dependence in the presence of Co2+. The AP site cleavage activity increases from 0.1 to 0.5 mM and then rapidly decreases from 0.5 to 10 mM CoCl2
Co2+
-
required for activity
KCl
the enzyme requires a low ionic strength
KCl
the enzyme requires high ionic strength, 200 mM KCl
Mg2+
-
stimulates
Mg2+
maximally stimulated in the presence of Mg2+
Mg2+
the enzyme requires the presence of Mg2+ and, to lesser extent, Ca2+ and Mn2+ for the DNA repair activity
Mg2+
the enzyme requires the presence of Mg2+ and, to lesser extent, Ca2+ and Mn2+ for the DNA repair activity. Under pH 7.6 optimal cation concentrations for MtbXthA-catalyzed activities are 2 mM MgCl2 and/or 0.5 mM MnCl2
Mg2+
Tequatrovirus T4
-
absolutely required for nicking activity
Mn2+
-
stimulates
Mn2+
-
0.01 M can replace Mg2+
Mn2+
the enzyme requires the presence of Mg2+ and, to lesser extent, Ca2+ and Mn2+ for the DNA repair activity. Depending on pH, the enzyme requires different concentrations of divalent cations: 0.5 mM MnCl2 at pH 7.6 and 10 mM at pH 6.5. It exhibits a non-linear dependence in the presence of Mn2+. The AP site cleavage activity increases from 0.1 to 0.5 mM and then rapidly decreases from 0.5 to 10 mM MnCl2. 10 mM MnCl2 is optimal
Mn2+
the enzyme requires the presence of Mg2+ and, to lesser extent, Ca2+ and Mn2+ for the DNA repair activity. Depending on pH, the enzyme requires different concentrations of divalent cations: 0.5 mM MnCl2 at pH 7.6 and 10 mM at pH 6.5. Under pH 7.6 optimal cation concentrations for MtbXthA-catalyzed activities are 2 mM MgCl2 and/or 0.5 mM MnCl2
Mn2+
-
required for activity
Mn2+
contains Mn2+, the enzyme with Mn2+ and Zn2+ is more active than with only Zn2+
Zn2+
-
dependent on
Zn2+
three zinc ions are identified at the active site
additional information
-
the enzyme shows no absolute requirement for divalent cations, no effect by Ca2+
additional information
not stimulated by Zn2+, Co2+
additional information
-
not stimulated by Zn2+, Co2+
additional information
the enzyme shows metal ion independent 3'->5' exonuclease activity
additional information
-
the enzyme shows metal ion independent 3'->5' exonuclease activity
additional information
the presence of Zn2+, Fe2+ and Ni2+ does not significantly stimulate AP endonuclease activity
additional information
the presence of Zn2+, Fe2+ and Ni2+ does not significantly stimulate AP endonuclease activity
additional information
-
the presence of Zn2+, Fe2+ and Ni2+ does not significantly stimulate AP endonuclease activity
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malfunction
-
phenotype with reduced DNA repair activity of enzyme-deficient mutant strain BW527, overview
malfunction
-
phenotype with reduced DNA repair activity of enzyme-deficient mutant strain BW527, overview
-
metabolism
the presence of this enzyme, which is part of the DNA base excision repair pathway, suggests that thermophiles use a mechanism similar to that used by mesophiles to deal with the large number of abasic sites that arise in their chromosomes due to the increased rates of DNA damage at elevated temperatures
metabolism
-
the presence of this enzyme, which is part of the DNA base excision repair pathway, suggests that thermophiles use a mechanism similar to that used by mesophiles to deal with the large number of abasic sites that arise in their chromosomes due to the increased rates of DNA damage at elevated temperatures
-
physiological function
-
endonuclease IV recognizes and cleaves DNA on the 5' side of various oxidatively damaged bases via nucleotide incision repair (NIR)
physiological function
-
endonuclease IV is the main base excision repair enzyme involved in DNA damage induced by UVA radiation and stannous chloride
physiological function
-
in the nucleotide incision repair pathway, an apurinic/apyrimidinic endonuclease incises duplex DNA 5' next to oxidatively damaged nucleotide. The multifunctional Escherichia coli endonuclease IV is involved in both base excision repair and nucleotide incision repair pathways, overview
physiological function
-
in vitro endonuclease activity of the Escherichia coli McrA homologue that specifically cleaves S-modified DNA
physiological function
the enzyme plays a crucial role in defense against oxidative stress
physiological function
key enzyme involved in the repair of abasic sites and DNA strand breaks
physiological function
-
in vitro endonuclease activity of the Escherichia coli McrA homologue that specifically cleaves S-modified DNA
-
physiological function
-
key enzyme involved in the repair of abasic sites and DNA strand breaks
-
physiological function
-
the enzyme plays a crucial role in defense against oxidative stress
-
physiological function
-
endonuclease IV recognizes and cleaves DNA on the 5' side of various oxidatively damaged bases via nucleotide incision repair (NIR)
-
physiological function
-
endonuclease IV is the main base excision repair enzyme involved in DNA damage induced by UVA radiation and stannous chloride
-
physiological function
-
in the nucleotide incision repair pathway, an apurinic/apyrimidinic endonuclease incises duplex DNA 5' next to oxidatively damaged nucleotide. The multifunctional Escherichia coli endonuclease IV is involved in both base excision repair and nucleotide incision repair pathways, overview
-
additional information
Tequatrovirus T4
-
a protruding loop containing a nuclease-associated modular domain 3 element is likely to be involved in substrate binding, as well as residues forming a separate nucleic acid binding surface adjacent to the active site. EndoII may bind its substrate inefficiently across the two sites in the dimer, offering a plausible explanation for the catalytic preponderance of single-strand nicks
additional information
-
Nfo catalyzed nucleotide incision and 3'-5' exonuclease activities are genetically coupled. modeling of nucleotide incision coupled to 3'-5' exonuclease activity preventing formation of lethal double-strand breaks when repairing bi-stranded clustered DNA damage, overview
additional information
-
Nfo catalyzed nucleotide incision and 3'-5' exonuclease activities are genetically coupled. modeling of nucleotide incision coupled to 3'-5' exonuclease activity preventing formation of lethal double-strand breaks when repairing bi-stranded clustered DNA damage, overview
-
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Sadowski, P.D.; Hurwitz, J.
Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli
J. Biol. Chem.
244
6192-6198
1969
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Friedberg, E.C.; Hadi, S.M.; Goldthwait, D.A.
Endonuclease II of Escherichia coli. II. Enzyme properties and studies on the degradation of alkylated and native deoxyribonucleic acid
J. Biol. Chem.
244
5879-5889
1969
Escherichia coli
brenda
Carlson, K.; Lagerback, P.; Nystrom, A.C.
Bacteriophage T4 endonuclease II: concerted single-strand nicks yield double-strand cleavage
Mol. Microbiol.
52
1403-1411
2004
Tequatrovirus T4
brenda
Souza, L.L.; Eduardo, I.R.; Padula, M.; Leitao, A.C.
Endonuclease IV and exonuclease III are involved in the repair and mutagenesis of DNA lesions induced by UVB in Escherichia coli
Mutagenesis
21
125-130
2006
Escherichia coli (P0A6C1), Escherichia coli
brenda
Hirano, N.; Ohshima, H.; Takahashi, H.
Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis
Nucleic Acids Res.
34
4743-4751
2006
Tequatrovirus T4
brenda
Ohshima, H.; Hirano, N.; Takahashi, H.
A hexanucleotide sequence (dC1-dC6 tract) restricts the dC-specific cleavage of single-stranded DNA by endonuclease IV of bacteriophage T4
Nucleic Acids Res.
35
6681-6689
2007
Tequatrovirus T4
brenda
Hirano, N.; Ohshima, H.; Sakashita, H.; Takahashi, H.
The Ser176 of T4 endonuclease IV is crucial for the restricted and polarized dC-specific cleavage of single-stranded DNA implicated in restriction of dC-containing DNA in host Escherichia coli
Nucleic Acids Res.
35
6692-6700
2007
Tequatrovirus T4
brenda
Ivanov, I.; Tainer, J.A.; McCammon, J.A.
Unraveling the three-metal-ion catalytic mechanism of the DNA repair enzyme endonuclease IV
Proc. Natl. Acad. Sci. USA
104
1465-1470
2007
Escherichia coli (P0A6C1)
brenda
Back, J.H.; Chung, J.H.; Park, J.H.; Han, Y.S.
A versatile endonuclease IV from Thermus thermophilus has uracil-excising and 3-5 exonuclease activity
Biochem. Biophys. Res. Commun.
346
889-895
2006
Thermus thermophilus (Q72KH8), Thermus thermophilus
brenda
Hughes, R.C.; Tomanicek, S.J.; Ng, J.D.; Coates, L.
Purification, crystallization and preliminary crystallographic analysis of a thermostable endonuclease IV from Thermotoga maritima
Acta Crystallogr. Sect. F
65
1317-1319
2009
Thermotoga maritima (Q9WYJ7), Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 (Q9WYJ7)
brenda
Motta, E.S.; Souza-Santos, P.T.; Cassiano, T.R.; Dantas, F.J.; Caldeira-de-Araujo, A.; De Mattos, J.C.
Endonuclease IV is the main base excision repair enzyme involved in DNA damage induced by UVA radiation and stannous chloride
J. Biomed. Biotechnol.
2010
376218
2010
Escherichia coli, Escherichia coli AB1157
brenda
Andersson, C.E.; Lagerbaeck, P.; Carlson, K.
Structure of bacteriophage T4 endonuclease II mutant E118A, a tetrameric GIY-YIG enzyme
J. Mol. Biol.
397
1003-1016
2010
Tequatrovirus T4
brenda
Golan, G.; Ishchenko, A.; Khassenov, B.; Shoham, G.; Saparbaev, M.
Coupling of the nucleotide incision and 3'-5' exonuclease activities in Escherichia coli endonuclease IV: Structural and genetic evidences
Mutat. Res.
685
70-79
2010
Escherichia coli, Escherichia coli AB1157
brenda
Liu, G.; Ou, H.; Wang, T.; Li, L.; Tan, H.; Zhou, X.; Rajakumar, K.; Deng, Z.; He, X.
Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA
PLoS Genet.
6
1-13
2010
Streptomyces coelicolor, Streptomyces coelicolor A(3)2
brenda
Xie, J.J.; Liu, X.P.; Han, Z.; Yuan, H.; Wang, Y.; Hou, J.L.; Liu, J.H.
Chlamydophila pneumoniae endonuclease IV prefers to remove mismatched 3 ribonucleotides: implication in proofreading mismatched 3-terminal nucleotides in short-patch repair synthesis
DNA Repair
12
140-147
2013
Chlamydia pneumoniae
brenda
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Thermotoga maritima (Q9WYJ7), Thermotoga maritima DSM 3109 (Q9WYJ7)
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PLoS ONE
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Mycobacterium tuberculosis (P9WQ13), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WQ13)
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Haas, B.; Sandigursky, M.; Tainer, J.; Franklin, W.; Cunningham, R.
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Thermotoga maritima (Q9WYJ7), Thermotoga maritima, Thermotoga maritima DSM 3109 (Q9WYJ7)
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Endonuclease IV cleaves apurinic/apyrimidinic sites in single-stranded DNA and its application for biosensing
Analyst
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Homo sapiens
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Zhang, W.; Xu, Y.; Yan, M.; Li, S.; Wang, H.; Yang, H.; Zhou, W.; Rao, Z.
Crystal structure of the apurinic/apyrimidinic endonuclease IV from Mycobacterium tuberculosis
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2018
Mycobacterium tuberculosis (P9WQ13), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WQ13)
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Abeldenov, S.; Talhaoui, I.; Zharkov, D.O.; Ishchenko, A.A.; Ramanculov, E.; Saparbaev, M.; Khassenov, B.
Characterization of DNA substrate specificities of apurinic/apyrimidinic endonucleases from Mycobacterium tuberculosis
DNA Repair
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1-16
2015
Mycobacterium tuberculosis (P96273), Mycobacterium tuberculosis (P9WQ13), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P96273), Mycobacterium tuberculosis H37Rv (P9WQ13)
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Wang, W.W.; Zhou, H.; Xie, J.J.; Yi, G.S.; He, J.H.; Wang, F.P.; Xiao, X.; Liu, X.P.
Thermococcus eurythermalis endonuclease IV can cleave various apurinic/apyrimidinic site analogues in ssDNA and dsDNA
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Thermococcus eurythermalis
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